Mutagenetix > Incidental Mutation

Incidental Mutation 'R4995:Muc4'

385212

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

042589-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R4995 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32735886-32782391 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 32754214 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 1363 (S1363T)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106] [ENSMUST00000132475]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000096106
AA Change: S1363T

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: S1363T

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000132475

SMART Domains

Protein: ENSMUSP00000119029
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	38	45	N/A	INTRINSIC
low complexity region	72	84	N/A	INTRINSIC
low complexity region	99	127	N/A	INTRINSIC
low complexity region	148	173	N/A	INTRINSIC
low complexity region	192	224	N/A	INTRINSIC
low complexity region	283	293	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135753

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142355

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.6%

Validation Efficiency

99% (86/87)

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921504E06Rik	G	A	2: 19,494,184 (GRCm38)	Q333*	probably null	Het
4930505A04Rik	C	T	11: 30,426,349 (GRCm38)	V173M	probably damaging	Het
Acvrl1	G	A	15: 101,135,860 (GRCm38)	R141H	probably benign	Het
Adprm	A	G	11: 67,041,610 (GRCm38)	F158L	possibly damaging	Het
Aldoart2	C	T	12: 55,566,253 (GRCm38)	T321M	probably benign	Het
Ap3m2	A	G	8: 22,803,776 (GRCm38)	V86A	probably benign	Het
Arhgef19	T	A	4: 141,247,515 (GRCm38)		probably null	Het
Armc4	G	A	18: 7,223,663 (GRCm38)	T460M	probably damaging	Het
Bcl2l12	T	G	7: 44,994,191 (GRCm38)		probably null	Het
Bptf	T	C	11: 107,054,565 (GRCm38)	Q2501R	probably damaging	Het
C230029F24Rik	A	T	1: 49,338,136 (GRCm38)		noncoding transcript	Het
C7	C	T	15: 5,049,592 (GRCm38)	G78D	probably damaging	Het
Caly	T	C	7: 140,070,625 (GRCm38)	T135A	probably benign	Het
Cbl	A	G	9: 44,153,811 (GRCm38)	M740T	possibly damaging	Het
Cbx4	A	G	11: 119,081,211 (GRCm38)	V446A	probably benign	Het
Celsr1	C	A	15: 85,937,911 (GRCm38)	R1735L	probably damaging	Het
Cep250	A	G	2: 155,988,316 (GRCm38)	D135G	probably damaging	Het
Cgn	T	C	3: 94,779,936 (GRCm38)	T19A	probably damaging	Het
Chic2	A	T	5: 75,044,204 (GRCm38)	V32D	probably damaging	Het
Cntln	A	G	4: 85,049,883 (GRCm38)	K780E	probably benign	Het
Col8a2	A	T	4: 126,310,788 (GRCm38)	D197V	probably damaging	Het
Crot	A	T	5: 8,974,000 (GRCm38)	V372E	probably damaging	Het
Cyb561d2	C	T	9: 107,541,548 (GRCm38)	V26M	probably damaging	Het
Ddx5	G	A	11: 106,785,236 (GRCm38)	T237I	probably damaging	Het
Dmxl2	G	T	9: 54,501,441 (GRCm38)		probably benign	Het
Dock8	T	A	19: 25,158,383 (GRCm38)	S1188R	probably benign	Het
Ehbp1	T	A	11: 22,101,073 (GRCm38)	H493L	probably damaging	Het
Eif5b	T	A	1: 38,051,711 (GRCm38)	*1217K	probably null	Het
Eprs	A	G	1: 185,410,139 (GRCm38)		probably benign	Het
Etfdh	T	C	3: 79,605,788 (GRCm38)	D376G	probably benign	Het
Fam186a	T	C	15: 99,945,099 (GRCm38)	Q1088R	probably benign	Het
Fbxw16	G	T	9: 109,441,250 (GRCm38)	T141N	probably damaging	Het
Fgf11	G	A	11: 69,798,759 (GRCm38)	H138Y	probably damaging	Het
Gm1673	G	A	5: 33,984,926 (GRCm38)	R79H	probably damaging	Het
Htra3	T	C	5: 35,671,074 (GRCm38)	E154G	probably damaging	Het
Hydin	T	A	8: 110,569,642 (GRCm38)	V3601D	probably damaging	Het
Jup	G	T	11: 100,379,541 (GRCm38)	S380*	probably null	Het
Klrg1	T	A	6: 122,278,275 (GRCm38)	D66V	probably benign	Het
Llgl1	C	T	11: 60,709,724 (GRCm38)	A633V	probably benign	Het
Lmln	T	A	16: 33,074,097 (GRCm38)	Y203*	probably null	Het
Lrrc58	T	G	16: 37,877,056 (GRCm38)	C165G	probably benign	Het
Lss	T	C	10: 76,547,537 (GRCm38)	V557A	probably benign	Het
Mast4	T	C	13: 102,905,754 (GRCm38)		probably benign	Het
Med13l	C	A	5: 118,730,949 (GRCm38)	P754Q	possibly damaging	Het
Mga	C	T	2: 119,932,582 (GRCm38)	R1240*	probably null	Het
Mgat5b	T	A	11: 116,974,199 (GRCm38)		probably null	Het
Mtor	A	G	4: 148,525,752 (GRCm38)	D1572G	probably damaging	Het
Myo18b	A	G	5: 112,760,392 (GRCm38)	V2005A	probably damaging	Het
Myo1e	G	A	9: 70,353,272 (GRCm38)	D571N	probably benign	Het
Mypn	T	C	10: 63,119,968 (GRCm38)		probably null	Het
Ndufb10	T	C	17: 24,722,757 (GRCm38)		probably null	Het
Nelfb	G	T	2: 25,206,196 (GRCm38)	D300E	probably benign	Het
Olfr1389	T	A	11: 49,430,655 (GRCm38)	Y60N	probably damaging	Het
Olfr1507	A	T	14: 52,490,531 (GRCm38)	C61*	probably null	Het
Olfr625-ps1	T	A	7: 103,683,367 (GRCm38)	D206E	probably damaging	Het
Olfr668	T	A	7: 104,925,735 (GRCm38)	T10S	probably benign	Het
Pcdha11	C	T	18: 37,011,027 (GRCm38)	T57M	probably benign	Het
Pkp1	A	T	1: 135,880,855 (GRCm38)	I458N	possibly damaging	Het
Prr12	T	A	7: 45,051,229 (GRCm38)		probably benign	Het
Prrc2c	A	T	1: 162,705,310 (GRCm38)		probably benign	Het
Psd4	T	C	2: 24,397,247 (GRCm38)	F397S	probably benign	Het
Pygm	T	C	19: 6,398,139 (GRCm38)	I737T	probably damaging	Het
Rfx1	T	A	8: 84,080,114 (GRCm38)		probably null	Het
Rsl1	A	G	13: 67,182,249 (GRCm38)	T254A	possibly damaging	Het
Sh3rf3	A	G	10: 59,086,824 (GRCm38)	Q574R	probably benign	Het
Spire1	T	C	18: 67,552,779 (GRCm38)		probably null	Het
St6galnac4	G	A	2: 32,594,063 (GRCm38)	G91D	probably damaging	Het
Sytl2	T	C	7: 90,382,257 (GRCm38)		probably benign	Het
Tbpl2	T	A	2: 24,093,860 (GRCm38)	K188N	possibly damaging	Het
Tenm3	A	T	8: 48,229,137 (GRCm38)	M2486K	possibly damaging	Het
Tgoln1	A	C	6: 72,616,140 (GRCm38)	V119G	possibly damaging	Het
Tpgs1	A	T	10: 79,669,491 (GRCm38)	N28Y	probably benign	Het
U2surp	A	C	9: 95,462,794 (GRCm38)		probably benign	Het
Vmn2r103	A	G	17: 19,773,511 (GRCm38)	H50R	probably benign	Het
Vmn2r19	A	G	6: 123,329,910 (GRCm38)	N459S	probably benign	Het
Vmn2r72	T	C	7: 85,738,485 (GRCm38)	S624G	probably damaging	Het
Vps13c	A	G	9: 67,919,321 (GRCm38)	T1415A	probably benign	Het
Vwa5b1	G	A	4: 138,608,843 (GRCm38)	P147S	probably damaging	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,778,812 (GRCm38)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,753,185 (GRCm38)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,750,910 (GRCm38)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,754,347 (GRCm38)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,849 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,848 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,823 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,755,805 (GRCm38)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,753,955 (GRCm38)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,754,071 (GRCm38)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,763,966 (GRCm38)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,750,655 (GRCm38)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,756,627 (GRCm38)	missense	unknown
IGL01625:Muc4	APN	16	32,755,544 (GRCm38)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,736,402 (GRCm38)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,761,348 (GRCm38)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,753,196 (GRCm38)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,751,426 (GRCm38)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,754,265 (GRCm38)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,777,649 (GRCm38)	splice site	probably benign
IGL02210:Muc4	APN	16	32,752,254 (GRCm38)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,750,850 (GRCm38)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,752,076 (GRCm38)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,756,129 (GRCm38)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,775,819 (GRCm38)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,755,282 (GRCm38)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,751,648 (GRCm38)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,750,394 (GRCm38)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,752,048 (GRCm38)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,751,948 (GRCm38)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,751,439 (GRCm38)	nonsense	probably null
IGL03335:Muc4	APN	16	32,753,021 (GRCm38)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,754,318 (GRCm38)	missense	probably benign	0.01
multisplendored	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,770,394 (GRCm38)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,760,251 (GRCm38)	splice site	probably benign
BB004:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
BB014:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
IGL02835:Muc4	UTSW	16	32,763,945 (GRCm38)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,760,248 (GRCm38)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,754,796 (GRCm38)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,756,017 (GRCm38)	missense	unknown
R0119:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,771,604 (GRCm38)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,765,746 (GRCm38)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,750,195 (GRCm38)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,752,905 (GRCm38)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,762,536 (GRCm38)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,751,069 (GRCm38)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,751,313 (GRCm38)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,756,746 (GRCm38)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,752,107 (GRCm38)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,751,670 (GRCm38)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,769,827 (GRCm38)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,769,847 (GRCm38)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,778,955 (GRCm38)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,752,002 (GRCm38)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,756,196 (GRCm38)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,750,525 (GRCm38)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,753,020 (GRCm38)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,752,233 (GRCm38)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,750,349 (GRCm38)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,753,595 (GRCm38)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,755,501 (GRCm38)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,750,986 (GRCm38)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,756,902 (GRCm38)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,755,933 (GRCm38)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,756,251 (GRCm38)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,756,341 (GRCm38)	missense	unknown
R1928:Muc4	UTSW	16	32,750,532 (GRCm38)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,750,642 (GRCm38)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,751,303 (GRCm38)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,752,254 (GRCm38)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,752,220 (GRCm38)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,756,409 (GRCm38)	missense	unknown
R2121:Muc4	UTSW	16	32,760,238 (GRCm38)	missense	unknown
R2139:Muc4	UTSW	16	32,761,225 (GRCm38)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,750,476 (GRCm38)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,766,942 (GRCm38)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,755,891 (GRCm38)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,757,472 (GRCm38)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,755,273 (GRCm38)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,751,051 (GRCm38)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,755,826 (GRCm38)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,770,292 (GRCm38)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,756,701 (GRCm38)	missense	unknown
R4523:Muc4	UTSW	16	32,736,336 (GRCm38)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,755,843 (GRCm38)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,757,058 (GRCm38)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,769,277 (GRCm38)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,755,846 (GRCm38)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,751,214 (GRCm38)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,775,903 (GRCm38)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,753,625 (GRCm38)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,751,747 (GRCm38)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,756,098 (GRCm38)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,754,319 (GRCm38)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,041 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,756,296 (GRCm38)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,757,058 (GRCm38)	nonsense	probably null
R5161:Muc4	UTSW	16	32,762,521 (GRCm38)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,751,738 (GRCm38)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,751,666 (GRCm38)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,761,261 (GRCm38)	missense	unknown
R5567:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5570:Muc4	UTSW	16	32,777,692 (GRCm38)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,750,782 (GRCm38)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,753,720 (GRCm38)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,776,807 (GRCm38)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,736,241 (GRCm38)	nonsense	probably null
R5708:Muc4	UTSW	16	32,754,769 (GRCm38)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,751,916 (GRCm38)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,774,839 (GRCm38)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,751,295 (GRCm38)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,751,705 (GRCm38)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,753,052 (GRCm38)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,756,278 (GRCm38)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,759,308 (GRCm38)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,775,783 (GRCm38)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,756,795 (GRCm38)	missense	unknown
R6144:Muc4	UTSW	16	32,766,924 (GRCm38)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,736,140 (GRCm38)	start gained	probably benign
R6268:Muc4	UTSW	16	32,768,767 (GRCm38)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,752,035 (GRCm38)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,753,946 (GRCm38)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,754,358 (GRCm38)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,767,351 (GRCm38)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,736,243 (GRCm38)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,778,946 (GRCm38)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,751,789 (GRCm38)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,759,320 (GRCm38)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,782,008 (GRCm38)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,753,964 (GRCm38)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,766,938 (GRCm38)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,775,803 (GRCm38)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,762,518 (GRCm38)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,751,999 (GRCm38)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,751,810 (GRCm38)	nonsense	probably null
R7033:Muc4	UTSW	16	32,756,324 (GRCm38)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,757,091 (GRCm38)	missense
R7123:Muc4	UTSW	16	32,750,691 (GRCm38)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,762,488 (GRCm38)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,756,461 (GRCm38)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,755,336 (GRCm38)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,755,366 (GRCm38)	missense	unknown
R7381:Muc4	UTSW	16	32,780,915 (GRCm38)	missense
R7411:Muc4	UTSW	16	32,751,322 (GRCm38)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,766,950 (GRCm38)	missense
R7539:Muc4	UTSW	16	32,756,396 (GRCm38)	missense
R7544:Muc4	UTSW	16	32,736,198 (GRCm38)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,765,702 (GRCm38)	missense
R7596:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,752,361 (GRCm38)	nonsense	probably null
R7639:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,760,105 (GRCm38)	missense
R7651:Muc4	UTSW	16	32,756,575 (GRCm38)	missense
R7688:Muc4	UTSW	16	32,751,460 (GRCm38)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,753,011 (GRCm38)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,768,734 (GRCm38)	missense
R7763:Muc4	UTSW	16	32,753,311 (GRCm38)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,756,194 (GRCm38)	missense	unknown
R7787:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,751,321 (GRCm38)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,771,637 (GRCm38)	missense
R7930:Muc4	UTSW	16	32,754,078 (GRCm38)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,756,749 (GRCm38)	missense
R8096:Muc4	UTSW	16	32,755,390 (GRCm38)	missense	unknown
R8115:Muc4	UTSW	16	32,755,304 (GRCm38)	missense	unknown
R8162:Muc4	UTSW	16	32,750,379 (GRCm38)	missense	unknown
R8220:Muc4	UTSW	16	32,755,327 (GRCm38)	missense	unknown
R8260:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,754,316 (GRCm38)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,755,897 (GRCm38)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,752,401 (GRCm38)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,753,508 (GRCm38)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,752,993 (GRCm38)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,755,255 (GRCm38)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,753,930 (GRCm38)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,781,986 (GRCm38)	missense
R8845:Muc4	UTSW	16	32,756,515 (GRCm38)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,750,643 (GRCm38)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,751,462 (GRCm38)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,753,994 (GRCm38)	missense	probably benign	0.00
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8990:Muc4	UTSW	16	32,752,209 (GRCm38)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,762,536 (GRCm38)	missense
R9034:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,754,076 (GRCm38)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,757,468 (GRCm38)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,768,728 (GRCm38)	missense
R9336:Muc4	UTSW	16	32,750,938 (GRCm38)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,751,153 (GRCm38)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,756,618 (GRCm38)	missense
R9429:Muc4	UTSW	16	32,755,724 (GRCm38)	missense	unknown
R9570:Muc4	UTSW	16	32,750,877 (GRCm38)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,756,860 (GRCm38)	missense
R9696:Muc4	UTSW	16	32,753,284 (GRCm38)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,770,270 (GRCm38)	missense
R9743:Muc4	UTSW	16	32,780,824 (GRCm38)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,751,858 (GRCm38)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,755,247 (GRCm38)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,751,825 (GRCm38)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,755,481 (GRCm38)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,759,319 (GRCm38)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,756,076 (GRCm38)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,768,730 (GRCm38)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,767,393 (GRCm38)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,774,862 (GRCm38)	missense
Z1177:Muc4	UTSW	16	32,767,394 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- AGCAGCTCAGCTCCAAGTAC -3'
(R):5'- TGTGGTCACCTCTGCATTGG -3'

Sequencing Primer
(F):5'- ATGTTATCCACAACATCCTCCACTC -3'
(R):5'- AGGATTGGTGCTGGTCCCC -3'

Posted On

2016-05-10