Incidental Mutation 'R4984:Pde4d'
| ID |
385797 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Pde4d
|
| Ensembl Gene |
ENSMUSG00000021699 |
| Gene Name |
phosphodiesterase 4D, cAMP specific |
| Synonyms |
dunce, Dpde3, 9630011N22Rik |
| MMRRC Submission |
042578-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R4984 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
13 |
| Chromosomal Location |
108449948-109953461 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 109740464 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Leucine to Proline
at position 173
(L173P)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000112991
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000074103]
[ENSMUST00000079975]
[ENSMUST00000119507]
[ENSMUST00000120671]
[ENSMUST00000122041]
[ENSMUST00000135275]
[ENSMUST00000177907]
|
| AlphaFold |
Q01063 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000074103
AA Change: L48P
PolyPhen 2
Score 0.137 (Sensitivity: 0.92; Specificity: 0.86)
|
| SMART Domains |
Protein: ENSMUSP00000073742
Gene: ENSMUSG00000021699
AA Change: L48P
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
8 |
18 |
N/A |
INTRINSIC |
|
HDc
|
329 |
504 |
1.12e-2 |
SMART |
|
low complexity region
|
652 |
667 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000079975
AA Change: L68P
PolyPhen 2
Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000078891
Gene: ENSMUSG00000021699
AA Change: L68P
| Domain | Start | End | E-Value | Type |
|---|
|
HDc
|
349 |
524 |
1.12e-2 |
SMART |
|
low complexity region
|
672 |
687 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000119507
AA Change: L73P
PolyPhen 2
Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000114089
Gene: ENSMUSG00000021699
AA Change: L73P
| Domain | Start | End | E-Value | Type |
|---|
|
HDc
|
354 |
529 |
1.12e-2 |
SMART |
|
low complexity region
|
677 |
692 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000120671
AA Change: L173P
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000112991
Gene: ENSMUSG00000021699
AA Change: L173P
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
45 |
84 |
N/A |
INTRINSIC |
|
HDc
|
454 |
629 |
1.12e-2 |
SMART |
|
low complexity region
|
777 |
792 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000122041
AA Change: L117P
PolyPhen 2
Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000113488
Gene: ENSMUSG00000021699
AA Change: L117P
| Domain | Start | End | E-Value | Type |
|---|
|
HDc
|
398 |
573 |
1.12e-2 |
SMART |
|
low complexity region
|
721 |
736 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000134973
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000135275
AA Change: L70P
PolyPhen 2
Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000119583
Gene: ENSMUSG00000021699
AA Change: L70P
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
25 |
N/A |
INTRINSIC |
|
HDc
|
351 |
526 |
1.12e-2 |
SMART |
|
low complexity region
|
674 |
689 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000138938
|
| Predicted Effect |
unknown
Transcript: ENSMUST00000153234
AA Change: L123P
|
| SMART Domains |
Protein: ENSMUSP00000121592
Gene: ENSMUSG00000021699
AA Change: L123P
| Domain | Start | End | E-Value | Type |
|---|
|
PDB:1E9K|A
|
22 |
59 |
9e-18 |
PDB |
|
low complexity region
|
69 |
85 |
N/A |
INTRINSIC |
|
HDc
|
405 |
580 |
1.12e-2 |
SMART |
|
low complexity region
|
728 |
743 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000177907
AA Change: L117P
PolyPhen 2
Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000136485
Gene: ENSMUSG00000021699
AA Change: L117P
| Domain | Start | End | E-Value | Type |
|---|
|
HDc
|
398 |
573 |
1.12e-2 |
SMART |
|
low complexity region
|
721 |
736 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.8170 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 96.7%
- 20x: 93.4%
|
| Validation Efficiency |
98% (41/42) |
| MGI Phenotype |
PHENOTYPE: Homozygotes for targeted null mutations exhibit delayed growth, female infertility associated with impaired ovulation, and reduced postnatal viability. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 36 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adra2a |
T |
C |
19: 54,046,639 |
I142T |
probably damaging |
Het |
| B020004J07Rik |
G |
C |
4: 101,835,599 |
N401K |
possibly damaging |
Het |
| Cd109 |
T |
C |
9: 78,634,677 |
|
probably null |
Het |
| Cdc42bpg |
T |
A |
19: 6,316,223 |
I812N |
possibly damaging |
Het |
| Cftr |
A |
G |
6: 18,235,199 |
E479G |
possibly damaging |
Het |
| Cpb1 |
GTTT |
GTT |
3: 20,270,352 |
|
probably null |
Het |
| Crocc |
T |
C |
4: 141,034,452 |
E873G |
probably damaging |
Het |
| Dnah3 |
C |
T |
7: 119,928,779 |
V3827I |
probably benign |
Het |
| Dync1h1 |
C |
A |
12: 110,658,126 |
T3700N |
probably damaging |
Het |
| E230001N04Rik |
T |
C |
17: 28,523,806 |
|
noncoding transcript |
Het |
| Gpr89 |
T |
A |
3: 96,905,196 |
M10L |
probably benign |
Het |
| Kbtbd7 |
T |
C |
14: 79,427,162 |
Y145H |
probably damaging |
Het |
| Kcnh8 |
T |
A |
17: 52,877,967 |
Y426N |
probably damaging |
Het |
| Mipep |
A |
G |
14: 60,788,182 |
D129G |
possibly damaging |
Het |
| Muc15 |
C |
T |
2: 110,731,573 |
P118L |
probably damaging |
Het |
| Mug1 |
T |
C |
6: 121,838,617 |
|
probably benign |
Het |
| Nubp1 |
T |
A |
16: 10,421,244 |
D208E |
probably damaging |
Het |
| Olfr1192-ps1 |
A |
G |
2: 88,412,577 |
L27P |
probably damaging |
Het |
| Olfr1256 |
A |
T |
2: 89,835,813 |
V44D |
probably damaging |
Het |
| Olfr1286 |
A |
T |
2: 111,420,847 |
Y35N |
probably damaging |
Het |
| Olfr229 |
A |
G |
9: 39,910,610 |
D269G |
probably benign |
Het |
| Olfr651 |
T |
G |
7: 104,553,021 |
I34S |
probably benign |
Het |
| Pdcd5 |
A |
C |
7: 35,642,685 |
V166G |
probably damaging |
Het |
| Phldb2 |
T |
C |
16: 45,825,633 |
Y195C |
probably damaging |
Het |
| Pifo |
T |
A |
3: 106,001,494 |
|
probably benign |
Het |
| Pou4f1 |
T |
C |
14: 104,466,183 |
E271G |
unknown |
Het |
| Ppl |
T |
C |
16: 5,087,641 |
I1597V |
probably benign |
Het |
| Sorl1 |
C |
A |
9: 41,991,342 |
D1640Y |
probably damaging |
Het |
| Taf4b |
T |
C |
18: 14,835,816 |
L670P |
probably damaging |
Het |
| Tox3 |
TCTGCTGCTGCTGCTGCTG |
TCTGCTGCTGCTGCTG |
8: 90,248,642 |
|
probably benign |
Het |
| Ttc39b |
A |
T |
4: 83,242,209 |
I377K |
probably benign |
Het |
| Ttll3 |
A |
G |
6: 113,412,940 |
D762G |
probably benign |
Het |
| Ubp1 |
T |
A |
9: 113,959,392 |
I279K |
probably damaging |
Het |
| Vmn2r93 |
T |
A |
17: 18,313,127 |
|
probably null |
Het |
| Wdfy3 |
C |
T |
5: 101,943,119 |
D532N |
probably benign |
Het |
| Zfp644 |
A |
T |
5: 106,636,917 |
L588Q |
possibly damaging |
Het |
|
| Other mutations in Pde4d |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00465:Pde4d
|
APN |
13 |
109936687 |
missense |
possibly damaging |
0.69 |
|
IGL00792:Pde4d
|
APN |
13 |
109935395 |
missense |
possibly damaging |
0.85 |
|
IGL01014:Pde4d
|
APN |
13 |
109949502 |
missense |
probably damaging |
1.00 |
|
IGL01660:Pde4d
|
APN |
13 |
109938072 |
missense |
probably damaging |
1.00 |
|
IGL02233:Pde4d
|
APN |
13 |
109740550 |
missense |
probably damaging |
1.00 |
|
IGL02405:Pde4d
|
APN |
13 |
108860209 |
critical splice donor site |
probably null |
|
|
IGL02544:Pde4d
|
APN |
13 |
109740523 |
missense |
probably damaging |
1.00 |
|
IGL02885:Pde4d
|
APN |
13 |
109948261 |
missense |
probably damaging |
1.00 |
|
IGL03286:Pde4d
|
APN |
13 |
109954506 |
unclassified |
probably benign |
|
|
IGL03406:Pde4d
|
APN |
13 |
109954591 |
unclassified |
probably benign |
|
|
Heliosphere
|
UTSW |
13 |
109116942 |
missense |
probably benign |
|
|
Stubbs
|
UTSW |
13 |
109772722 |
intron |
probably benign |
|
|
IGL03055:Pde4d
|
UTSW |
13 |
109935345 |
missense |
probably damaging |
1.00 |
|
R0020:Pde4d
|
UTSW |
13 |
109954570 |
missense |
possibly damaging |
0.66 |
|
R0020:Pde4d
|
UTSW |
13 |
109954570 |
missense |
possibly damaging |
0.66 |
|
R0054:Pde4d
|
UTSW |
13 |
109740421 |
missense |
probably benign |
0.23 |
|
R0054:Pde4d
|
UTSW |
13 |
109740421 |
missense |
probably benign |
0.23 |
|
R0357:Pde4d
|
UTSW |
13 |
109951268 |
missense |
possibly damaging |
0.46 |
|
R0482:Pde4d
|
UTSW |
13 |
109936710 |
missense |
probably benign |
0.00 |
|
R0689:Pde4d
|
UTSW |
13 |
109740544 |
missense |
possibly damaging |
0.78 |
|
R0884:Pde4d
|
UTSW |
13 |
109950940 |
missense |
probably damaging |
0.99 |
|
R1169:Pde4d
|
UTSW |
13 |
109950928 |
splice site |
probably null |
|
|
R1225:Pde4d
|
UTSW |
13 |
109950221 |
missense |
probably benign |
0.04 |
|
R1246:Pde4d
|
UTSW |
13 |
109950973 |
missense |
probably damaging |
1.00 |
|
R1344:Pde4d
|
UTSW |
13 |
109950387 |
nonsense |
probably null |
|
|
R1351:Pde4d
|
UTSW |
13 |
109951275 |
missense |
possibly damaging |
0.46 |
|
R1371:Pde4d
|
UTSW |
13 |
109117061 |
missense |
probably benign |
0.00 |
|
R1418:Pde4d
|
UTSW |
13 |
109950387 |
nonsense |
probably null |
|
|
R2197:Pde4d
|
UTSW |
13 |
109948390 |
missense |
probably damaging |
1.00 |
|
R2440:Pde4d
|
UTSW |
13 |
109927197 |
intron |
probably benign |
|
|
R3114:Pde4d
|
UTSW |
13 |
109948258 |
missense |
probably damaging |
1.00 |
|
R3115:Pde4d
|
UTSW |
13 |
109948258 |
missense |
probably damaging |
1.00 |
|
R3722:Pde4d
|
UTSW |
13 |
109951332 |
nonsense |
probably null |
|
|
R3742:Pde4d
|
UTSW |
13 |
109740479 |
missense |
probably benign |
0.42 |
|
R3797:Pde4d
|
UTSW |
13 |
109632897 |
missense |
probably benign |
0.29 |
|
R3983:Pde4d
|
UTSW |
13 |
109740406 |
missense |
probably benign |
0.23 |
|
R4618:Pde4d
|
UTSW |
13 |
109933877 |
missense |
probably benign |
0.13 |
|
R4768:Pde4d
|
UTSW |
13 |
109933874 |
missense |
probably damaging |
1.00 |
|
R4795:Pde4d
|
UTSW |
13 |
109938171 |
intron |
probably benign |
|
|
R4824:Pde4d
|
UTSW |
13 |
109116866 |
missense |
probably benign |
0.00 |
|
R4942:Pde4d
|
UTSW |
13 |
108860199 |
missense |
probably benign |
0.00 |
|
R5180:Pde4d
|
UTSW |
13 |
109740473 |
missense |
probably benign |
0.13 |
|
R5267:Pde4d
|
UTSW |
13 |
109260809 |
intron |
probably benign |
|
|
R5311:Pde4d
|
UTSW |
13 |
109632864 |
missense |
probably benign |
0.02 |
|
R5311:Pde4d
|
UTSW |
13 |
109632865 |
missense |
probably benign |
|
|
R5376:Pde4d
|
UTSW |
13 |
109772644 |
missense |
probably benign |
0.00 |
|
R5551:Pde4d
|
UTSW |
13 |
109948396 |
critical splice donor site |
probably null |
|
|
R5753:Pde4d
|
UTSW |
13 |
109772722 |
intron |
probably benign |
|
|
R5754:Pde4d
|
UTSW |
13 |
109938013 |
missense |
probably damaging |
0.98 |
|
R5838:Pde4d
|
UTSW |
13 |
109740442 |
missense |
probably damaging |
0.99 |
|
R5864:Pde4d
|
UTSW |
13 |
109938048 |
missense |
probably benign |
0.00 |
|
R6039:Pde4d
|
UTSW |
13 |
109948342 |
missense |
probably damaging |
1.00 |
|
R6039:Pde4d
|
UTSW |
13 |
109948342 |
missense |
probably damaging |
1.00 |
|
R6049:Pde4d
|
UTSW |
13 |
109032585 |
nonsense |
probably null |
|
|
R6214:Pde4d
|
UTSW |
13 |
109949433 |
missense |
probably damaging |
1.00 |
|
R6215:Pde4d
|
UTSW |
13 |
109949433 |
missense |
probably damaging |
1.00 |
|
R6273:Pde4d
|
UTSW |
13 |
109950221 |
missense |
possibly damaging |
0.94 |
|
R6431:Pde4d
|
UTSW |
13 |
109601786 |
splice site |
probably null |
|
|
R6501:Pde4d
|
UTSW |
13 |
109116942 |
missense |
probably benign |
|
|
R6534:Pde4d
|
UTSW |
13 |
109632901 |
missense |
probably benign |
0.05 |
|
R6709:Pde4d
|
UTSW |
13 |
109948279 |
missense |
probably damaging |
1.00 |
|
R6722:Pde4d
|
UTSW |
13 |
109632898 |
nonsense |
probably null |
|
|
R7164:Pde4d
|
UTSW |
13 |
109032688 |
missense |
probably benign |
|
|
R7222:Pde4d
|
UTSW |
13 |
109757579 |
missense |
probably damaging |
1.00 |
|
R7417:Pde4d
|
UTSW |
13 |
109632788 |
splice site |
probably null |
|
|
R7489:Pde4d
|
UTSW |
13 |
109116767 |
missense |
unknown |
|
|
R7563:Pde4d
|
UTSW |
13 |
109951007 |
missense |
probably benign |
0.37 |
|
R7861:Pde4d
|
UTSW |
13 |
109935324 |
missense |
probably damaging |
0.99 |
|
R8167:Pde4d
|
UTSW |
13 |
109442321 |
missense |
probably benign |
0.00 |
|
R8197:Pde4d
|
UTSW |
13 |
109948336 |
missense |
probably damaging |
1.00 |
|
R8469:Pde4d
|
UTSW |
13 |
108860188 |
missense |
probably benign |
|
|
R8715:Pde4d
|
UTSW |
13 |
109935342 |
missense |
probably benign |
0.29 |
|
R8926:Pde4d
|
UTSW |
13 |
109938091 |
missense |
probably benign |
0.00 |
|
R9054:Pde4d
|
UTSW |
13 |
109935390 |
missense |
probably damaging |
0.96 |
|
R9406:Pde4d
|
UTSW |
13 |
109740530 |
missense |
probably damaging |
0.99 |
|
R9516:Pde4d
|
UTSW |
13 |
109260662 |
missense |
|
|
|
R9526:Pde4d
|
UTSW |
13 |
109935381 |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CACTACATCTCTAGTTCAACGTTG -3'
(R):5'- CCAGAGATGAATCTGCTCAGG -3'
Sequencing Primer
(F):5'- GCAAGAATCAGATGTAATATCTTCCC -3'
(R):5'- CAGAGATGAATCTGCTCAGGTTTTTC -3'
|
| Posted On |
2016-05-10 |
Incidental Mutation