Mutagenetix > Incidental Mutation

Incidental Mutation 'R4989:Smg1'

386040

Institutional Source

Beutler Lab

Gene Symbol

Smg1

Ensembl Gene

ENSMUSG00000030655

Gene Name

SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)

Synonyms

2610207I05Rik, 5430435M13Rik, C130002K18Rik

MMRRC Submission

042583-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4989 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

118131308-118243670 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 118208051 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 168 (A168T)

Ref Sequence

ENSEMBL: ENSMUSP00000137172 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032891] [ENSMUST00000179047]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000032891
AA Change: A192T

SMART Domains

Protein: ENSMUSP00000032891
Gene: ENSMUSG00000030655
AA Change: A192T

Domain	Start	End	E-Value	Type
low complexity region	42	55	N/A	INTRINSIC
SCOP:d1gw5a_	147	621	7e-7	SMART
Pfam:SMG1	629	1240	9.8e-249	PFAM
low complexity region	1540	1551	N/A	INTRINSIC
SCOP:d1gw5a_	1680	1942	8e-3	SMART
low complexity region	2125	2141	N/A	INTRINSIC
PI3Kc	2149	2493	7.93e-50	SMART
low complexity region	2759	2770	N/A	INTRINSIC
low complexity region	3425	3442	N/A	INTRINSIC
FATC	3626	3658	8.66e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000179047
AA Change: A168T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000137172
Gene: ENSMUSG00000030655
AA Change: A168T

Domain	Start	End	E-Value	Type
low complexity region	18	31	N/A	INTRINSIC
SCOP:d1gw5a_	123	282	7e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000179331

SMART Domains

Protein: ENSMUSP00000137592
Gene: ENSMUSG00000030655

Domain	Start	End	E-Value	Type
low complexity region	18	31	N/A	INTRINSIC
SCOP:d1gw5a_	71	545	1e-6	SMART
low complexity region	602	612	N/A	INTRINSIC
low complexity region	631	646	N/A	INTRINSIC
low complexity region	698	718	N/A	INTRINSIC
low complexity region	898	915	N/A	INTRINSIC
low complexity region	1135	1147	N/A	INTRINSIC
low complexity region	1464	1475	N/A	INTRINSIC
low complexity region	2049	2065	N/A	INTRINSIC
PI3Kc	2073	2417	7.93e-50	SMART
low complexity region	2683	2694	N/A	INTRINSIC
low complexity region	3349	3366	N/A	INTRINSIC
FATC	3550	3582	8.66e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000179847

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180009

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000208209

Meta Mutation Damage Score

0.0789

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.4%

Validation Efficiency

100% (115/115)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]
PHENOTYPE: Mice homozygous for a gene trap allele exhibit early embryonic lethality. Mice heteroygous for a gene trap allele exhibit abnormal tooth development, chronic inflammation, increased body weight, increased incidence of tumor formation and premature death. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 113 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	A	T	12: 71,164,547 (GRCm38)	E685V	possibly damaging	Het
2700049A03Rik	G	T	12: 71,164,546 (GRCm38)	E685*	probably null	Het
4931406P16Rik	A	C	7: 34,245,800 (GRCm38)	Y552D	probably damaging	Het
Acot6	A	G	12: 84,109,015 (GRCm38)	K246E	probably benign	Het
Actl11	T	A	9: 107,931,416 (GRCm38)	H979Q	probably damaging	Het
Adal	T	A	2: 121,155,549 (GRCm38)		probably benign	Het
Add2	G	A	6: 86,110,858 (GRCm38)	V596I	probably benign	Het
Adrb3	A	C	8: 27,227,770 (GRCm38)	M217R	probably damaging	Het
Akap2	T	A	4: 57,856,552 (GRCm38)	V668E	probably benign	Het
Ank1	T	C	8: 23,141,118 (GRCm38)		probably benign	Het
Ank2	A	T	3: 126,963,445 (GRCm38)	N1054K	possibly damaging	Het
Ankib1	T	C	5: 3,713,217 (GRCm38)	Y504C	probably damaging	Het
Ankrd29	T	C	18: 12,262,185 (GRCm38)	K217R	probably damaging	Het
Ankrd45	T	C	1: 161,155,306 (GRCm38)	V129A	probably damaging	Het
Aox4	A	T	1: 58,236,676 (GRCm38)	D389V	probably benign	Het
Aph1a	G	T	3: 95,895,531 (GRCm38)	G148W	probably damaging	Het
Arhgef26	G	A	3: 62,340,385 (GRCm38)	D297N	possibly damaging	Het
Atxn7l3b	C	A	10: 112,928,744 (GRCm38)		probably benign	Het
Auh	A	G	13: 52,841,029 (GRCm38)	S167P	probably damaging	Het
Bach1	A	T	16: 87,719,000 (GRCm38)	K143I	possibly damaging	Het
Bbx	T	A	16: 50,224,738 (GRCm38)	T487S	probably damaging	Het
Bche	T	G	3: 73,701,844 (GRCm38)	D83A	probably benign	Het
Bri3bp	G	T	5: 125,441,696 (GRCm38)		probably benign	Het
Cd3d	A	T	9: 44,984,998 (GRCm38)	E28D	probably damaging	Het
Cdc42bpa	A	G	1: 180,137,801 (GRCm38)	T1028A	probably damaging	Het
Celsr2	T	C	3: 108,412,629 (GRCm38)	I956V	possibly damaging	Het
Cep95	A	T	11: 106,816,654 (GRCm38)		probably null	Het
Cic	G	T	7: 25,287,110 (GRCm38)	G1289C	probably damaging	Het
Cndp1	A	G	18: 84,631,900 (GRCm38)	Y223H	probably damaging	Het
Cops8	A	G	1: 90,611,002 (GRCm38)	D51G	probably damaging	Het
Csgalnact1	T	C	8: 68,460,971 (GRCm38)	E194G	probably benign	Het
Ctsm	A	G	13: 61,538,962 (GRCm38)	Y39H	probably damaging	Het
Dhrs2	T	C	14: 55,237,265 (GRCm38)	V119A	probably damaging	Het
Drosha	T	A	15: 12,935,007 (GRCm38)	M1336K	probably benign	Het
Dsp	G	A	13: 38,197,702 (GRCm38)	D2808N	possibly damaging	Het
Ehmt1	G	T	2: 24,877,497 (GRCm38)	P135T	probably damaging	Het
Epas1	A	G	17: 86,809,454 (GRCm38)	N184S	probably damaging	Het
Erich3	C	T	3: 154,748,388 (GRCm38)	T597I	possibly damaging	Het
F10	T	C	8: 13,055,698 (GRCm38)	V421A	probably damaging	Het
Fabp3	C	T	4: 130,312,387 (GRCm38)	T57I	probably benign	Het
Fam227b	G	A	2: 126,116,123 (GRCm38)	P241S	probably damaging	Het
Fcgrt	C	T	7: 45,101,948 (GRCm38)	G192D	probably benign	Het
Fras1	A	G	5: 96,650,682 (GRCm38)	E1184G	possibly damaging	Het
Gm5155	A	G	7: 17,905,218 (GRCm38)		probably null	Het
Gm8989	A	T	7: 106,329,457 (GRCm38)		noncoding transcript	Het
Grn	C	A	11: 102,430,554 (GRCm38)		probably benign	Het
Hadh	A	T	3: 131,235,548 (GRCm38)	L274*	probably null	Het
Hus1	T	C	11: 9,006,027 (GRCm38)	S169G	probably damaging	Het
Hydin	A	T	8: 110,563,922 (GRCm38)	I3338F	possibly damaging	Het
Ighv11-1	T	G	12: 113,982,148 (GRCm38)	E28D	probably benign	Het
Kctd9	C	T	14: 67,729,356 (GRCm38)	T106I	probably damaging	Het
Krit1	A	T	5: 3,822,238 (GRCm38)	N421I	probably damaging	Het
Lamb1	A	G	12: 31,326,678 (GRCm38)	D1619G	probably damaging	Het
Lpar6	G	A	14: 73,238,707 (GRCm38)	C36Y	probably damaging	Het
Lrrc37a	G	C	11: 103,456,739 (GRCm38)	F3043L	unknown	Het
Lrrc46	A	T	11: 97,040,939 (GRCm38)	V19D	probably damaging	Het
Mapkap1	G	T	2: 34,581,291 (GRCm38)	S197I	probably damaging	Het
Mrps35	A	C	6: 147,060,147 (GRCm38)	K173N	possibly damaging	Het
Mtf2	A	G	5: 108,073,028 (GRCm38)		probably benign	Het
Mum1	T	C	10: 80,232,868 (GRCm38)	L282P	probably benign	Het
Ncf1	A	C	5: 134,223,413 (GRCm38)	D261E	probably damaging	Het
Notch1	C	T	2: 26,481,181 (GRCm38)	E298K	probably damaging	Het
Nrxn1	A	T	17: 90,620,846 (GRCm38)		probably benign	Het
Olfr110	G	T	17: 37,499,126 (GRCm38)	L158F	probably benign	Het
Olfr1274-ps	T	A	2: 90,400,763 (GRCm38)	M34K	probably benign	Het
Olfr195	T	C	16: 59,149,618 (GRCm38)	L256P	probably damaging	Het
Olfr46	C	A	7: 140,610,391 (GRCm38)	A75E	possibly damaging	Het
Olfr622	A	G	7: 103,640,101 (GRCm38)	F13S	probably damaging	Het
Otud4	G	A	8: 79,655,689 (GRCm38)	V176I	probably damaging	Het
Papolg	T	C	11: 23,873,919 (GRCm38)		probably null	Het
Pgk2	A	G	17: 40,207,511 (GRCm38)	V342A	probably damaging	Het
Phf2	G	A	13: 48,807,844 (GRCm38)	A790V	unknown	Het
Prkdc	T	C	16: 15,673,997 (GRCm38)	I602T	possibly damaging	Het
Prl3d3	T	C	13: 27,159,089 (GRCm38)	I86T	possibly damaging	Het
Prss8	G	T	7: 127,926,463 (GRCm38)	Q295K	probably benign	Het
Ptpre	C	T	7: 135,669,132 (GRCm38)	H346Y	probably benign	Het
Ptpro	T	A	6: 137,443,594 (GRCm38)	V1007D	probably damaging	Het
Qrfpr	A	G	3: 36,222,136 (GRCm38)	V35A	probably damaging	Het
Rpf1	A	G	3: 146,506,538 (GRCm38)	L349S	probably damaging	Het
Rpl41	G	T	10: 128,548,783 (GRCm38)		probably null	Het
Rprd2	G	C	3: 95,765,320 (GRCm38)	R924G	probably benign	Het
Serpini1	T	A	3: 75,614,488 (GRCm38)	N95K	probably benign	Het
Sfxn1	A	T	13: 54,088,914 (GRCm38)	T64S	probably benign	Het
Siae	T	G	9: 37,646,520 (GRCm38)	I541S	possibly damaging	Het
Slc25a4	T	A	8: 46,207,472 (GRCm38)	K296N	probably benign	Het
Slc37a1	A	G	17: 31,322,146 (GRCm38)	N204S	probably damaging	Het
Slc9c1	A	G	16: 45,593,437 (GRCm38)	N976S	probably benign	Het
Smarcal1	T	C	1: 72,632,860 (GRCm38)	S847P	possibly damaging	Het
Sptlc1	A	G	13: 53,351,656 (GRCm38)	I242T	probably damaging	Het
St8sia2	T	C	7: 73,966,961 (GRCm38)	I89V	possibly damaging	Het
Supt20	A	T	3: 54,695,134 (GRCm38)		probably benign	Het
Tep1	T	C	14: 50,839,000 (GRCm38)	D1659G	probably benign	Het
Tex10	C	A	4: 48,458,525 (GRCm38)		probably benign	Het
Thbs3	CAGAAG	CAG	3: 89,223,102 (GRCm38)		probably benign	Het
Tmbim6	C	A	15: 99,402,069 (GRCm38)	S22*	probably null	Het
Tmc2	A	T	2: 130,202,041 (GRCm38)	K65M	possibly damaging	Het
Tmod1	A	G	4: 46,090,872 (GRCm38)	S142G	probably damaging	Het
Tns2	C	T	15: 102,108,934 (GRCm38)	R281C	probably damaging	Het
Trmt6	T	C	2: 132,808,271 (GRCm38)	R349G	possibly damaging	Het
Ttn	C	T	2: 76,774,778 (GRCm38)	V16555I	probably benign	Het
Ttn	T	A	2: 76,707,242 (GRCm38)	T26454S	possibly damaging	Het
Ubr3	T	C	2: 70,020,446 (GRCm38)		probably benign	Het
Vmn1r17	A	C	6: 57,360,475 (GRCm38)	F253V	possibly damaging	Het
Vmn1r201	G	T	13: 22,475,452 (GRCm38)	A279S	possibly damaging	Het
Vmn2r17	A	T	5: 109,427,873 (GRCm38)	R203S	probably benign	Het
Vwa5a	A	G	9: 38,722,630 (GRCm38)	E43G	probably benign	Het
Wdr86	C	T	5: 24,712,845 (GRCm38)		probably null	Het
Wdsub1	A	T	2: 59,870,414 (GRCm38)		probably benign	Het
Zcchc11	T	A	4: 108,526,845 (GRCm38)		probably benign	Het
Zcchc7	T	C	4: 44,931,039 (GRCm38)	L76P	probably damaging	Het
Zfp108	G	T	7: 24,260,738 (GRCm38)	K251N	probably benign	Het
Zfp263	T	C	16: 3,749,128 (GRCm38)	C148R	probably damaging	Het
Zfp687	A	C	3: 95,010,386 (GRCm38)	F692V	probably damaging	Het

Other mutations in Smg1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Smg1	APN	7	118,198,271 (GRCm38)	utr 3 prime	probably benign
IGL00481:Smg1	APN	7	118,210,794 (GRCm38)	missense	possibly damaging	0.67
IGL00503:Smg1	APN	7	118,185,483 (GRCm38)	utr 3 prime	probably benign
IGL00927:Smg1	APN	7	118,140,632 (GRCm38)	missense	probably damaging	1.00
IGL01333:Smg1	APN	7	118,163,378 (GRCm38)	splice site	probably benign
IGL01344:Smg1	APN	7	118,190,836 (GRCm38)	utr 3 prime	probably benign
IGL01397:Smg1	APN	7	118,163,221 (GRCm38)	utr 3 prime	probably benign
IGL01403:Smg1	APN	7	118,158,132 (GRCm38)	utr 3 prime	probably benign
IGL01573:Smg1	APN	7	118,167,962 (GRCm38)	utr 3 prime	probably benign
IGL01872:Smg1	APN	7	118,148,944 (GRCm38)	utr 3 prime	probably benign
IGL02010:Smg1	APN	7	118,186,146 (GRCm38)	utr 3 prime	probably benign
IGL02158:Smg1	APN	7	118,212,946 (GRCm38)	missense	possibly damaging	0.77
IGL02268:Smg1	APN	7	118,182,541 (GRCm38)	missense	probably benign	0.19
IGL02314:Smg1	APN	7	118,154,709 (GRCm38)	utr 3 prime	probably benign
IGL02552:Smg1	APN	7	118,195,894 (GRCm38)	utr 3 prime	probably benign
IGL02577:Smg1	APN	7	118,203,122 (GRCm38)	missense	probably damaging	0.99
IGL02859:Smg1	APN	7	118,148,933 (GRCm38)	utr 3 prime	probably benign
IGL02890:Smg1	APN	7	118,185,501 (GRCm38)	utr 3 prime	probably benign
IGL02892:Smg1	APN	7	118,167,955 (GRCm38)	utr 3 prime	probably benign
IGL03119:Smg1	APN	7	118,195,113 (GRCm38)	utr 3 prime	probably benign
IGL03123:Smg1	APN	7	118,157,181 (GRCm38)	utr 3 prime	probably benign
IGL03128:Smg1	APN	7	118,203,059 (GRCm38)	missense	probably benign	0.03
IGL03184:Smg1	APN	7	118,180,380 (GRCm38)	missense	possibly damaging	0.86
PIT4508001:Smg1	UTSW	7	118,185,541 (GRCm38)	missense	unknown
R0010:Smg1	UTSW	7	118,171,859 (GRCm38)	utr 3 prime	probably benign
R0010:Smg1	UTSW	7	118,171,859 (GRCm38)	utr 3 prime	probably benign
R0025:Smg1	UTSW	7	118,212,443 (GRCm38)	missense	possibly damaging	0.92
R0025:Smg1	UTSW	7	118,212,443 (GRCm38)	missense	possibly damaging	0.92
R0098:Smg1	UTSW	7	118,145,467 (GRCm38)	missense	probably benign	0.02
R0139:Smg1	UTSW	7	118,152,675 (GRCm38)	critical splice donor site	probably null
R0371:Smg1	UTSW	7	118,168,300 (GRCm38)	utr 3 prime	probably benign
R0415:Smg1	UTSW	7	118,182,468 (GRCm38)	missense	probably benign	0.34
R0416:Smg1	UTSW	7	118,184,461 (GRCm38)	splice site	probably benign
R0423:Smg1	UTSW	7	118,176,880 (GRCm38)	missense	possibly damaging	0.53
R0600:Smg1	UTSW	7	118,160,383 (GRCm38)	utr 3 prime	probably benign
R0626:Smg1	UTSW	7	118,182,383 (GRCm38)	missense	possibly damaging	0.82
R0627:Smg1	UTSW	7	118,167,861 (GRCm38)	utr 3 prime	probably benign
R0727:Smg1	UTSW	7	118,166,422 (GRCm38)	utr 3 prime	probably benign
R0729:Smg1	UTSW	7	118,146,289 (GRCm38)	utr 3 prime	probably benign
R0841:Smg1	UTSW	7	118,143,301 (GRCm38)	missense	possibly damaging	0.96
R1114:Smg1	UTSW	7	118,159,790 (GRCm38)	utr 3 prime	probably benign
R1256:Smg1	UTSW	7	118,203,087 (GRCm38)	missense	probably damaging	1.00
R1298:Smg1	UTSW	7	118,168,211 (GRCm38)	utr 3 prime	probably benign
R1370:Smg1	UTSW	7	118,159,752 (GRCm38)	utr 3 prime	probably benign
R1591:Smg1	UTSW	7	118,156,919 (GRCm38)	utr 3 prime	probably benign
R1736:Smg1	UTSW	7	118,165,967 (GRCm38)	splice site	probably null
R1755:Smg1	UTSW	7	118,203,064 (GRCm38)	nonsense	probably null
R1765:Smg1	UTSW	7	118,139,715 (GRCm38)	missense	probably benign	0.03
R1789:Smg1	UTSW	7	118,145,798 (GRCm38)	missense	possibly damaging	0.73
R1845:Smg1	UTSW	7	118,154,622 (GRCm38)	utr 3 prime	probably benign
R1908:Smg1	UTSW	7	118,154,199 (GRCm38)	utr 3 prime	probably benign
R1909:Smg1	UTSW	7	118,154,199 (GRCm38)	utr 3 prime	probably benign
R1942:Smg1	UTSW	7	118,158,103 (GRCm38)	utr 3 prime	probably benign
R2064:Smg1	UTSW	7	118,156,867 (GRCm38)	utr 3 prime	probably benign
R2072:Smg1	UTSW	7	118,163,166 (GRCm38)	utr 3 prime	probably benign
R2154:Smg1	UTSW	7	118,158,076 (GRCm38)	utr 3 prime	probably benign
R2895:Smg1	UTSW	7	118,189,143 (GRCm38)	utr 3 prime	probably benign
R2915:Smg1	UTSW	7	118,210,879 (GRCm38)	splice site	probably benign
R3416:Smg1	UTSW	7	118,148,853 (GRCm38)	utr 3 prime	probably benign
R3417:Smg1	UTSW	7	118,148,853 (GRCm38)	utr 3 prime	probably benign
R3873:Smg1	UTSW	7	118,154,662 (GRCm38)	utr 3 prime	probably benign
R4082:Smg1	UTSW	7	118,160,246 (GRCm38)	utr 3 prime	probably benign
R4230:Smg1	UTSW	7	118,148,733 (GRCm38)	critical splice donor site	probably null
R4304:Smg1	UTSW	7	118,139,518 (GRCm38)	missense	probably benign	0.03
R4549:Smg1	UTSW	7	118,159,683 (GRCm38)	utr 3 prime	probably benign
R4571:Smg1	UTSW	7	118,139,465 (GRCm38)	missense	possibly damaging	0.72
R4638:Smg1	UTSW	7	118,195,926 (GRCm38)	utr 3 prime	probably benign
R4642:Smg1	UTSW	7	118,154,264 (GRCm38)	utr 3 prime	probably benign
R4656:Smg1	UTSW	7	118,212,951 (GRCm38)	missense	probably benign	0.00
R4754:Smg1	UTSW	7	118,156,731 (GRCm38)	utr 3 prime	probably benign
R4798:Smg1	UTSW	7	118,180,474 (GRCm38)	missense	probably benign	0.32
R4906:Smg1	UTSW	7	118,152,408 (GRCm38)	utr 3 prime	probably benign
R4978:Smg1	UTSW	7	118,154,247 (GRCm38)	utr 3 prime	probably benign
R4989:Smg1	UTSW	7	118,158,100 (GRCm38)	utr 3 prime	probably benign
R5026:Smg1	UTSW	7	118,193,545 (GRCm38)	utr 3 prime	probably benign
R5124:Smg1	UTSW	7	118,213,012 (GRCm38)	missense	probably benign	0.00
R5318:Smg1	UTSW	7	118,160,204 (GRCm38)	utr 3 prime	probably benign
R5356:Smg1	UTSW	7	118,195,133 (GRCm38)	utr 3 prime	probably benign
R5404:Smg1	UTSW	7	118,206,908 (GRCm38)	missense	probably damaging	1.00
R5423:Smg1	UTSW	7	118,146,071 (GRCm38)	missense	possibly damaging	0.70
R5441:Smg1	UTSW	7	118,195,081 (GRCm38)	utr 3 prime	probably benign
R5490:Smg1	UTSW	7	118,139,436 (GRCm38)	missense	possibly damaging	0.86
R5541:Smg1	UTSW	7	118,157,163 (GRCm38)	utr 3 prime	probably benign
R5564:Smg1	UTSW	7	118,189,819 (GRCm38)	utr 3 prime	probably benign
R5580:Smg1	UTSW	7	118,148,902 (GRCm38)	utr 3 prime	probably benign
R5600:Smg1	UTSW	7	118,167,884 (GRCm38)	utr 3 prime	probably benign
R5628:Smg1	UTSW	7	118,154,701 (GRCm38)	utr 3 prime	probably benign
R5646:Smg1	UTSW	7	118,212,559 (GRCm38)	missense	probably benign	0.42
R5656:Smg1	UTSW	7	118,154,664 (GRCm38)	utr 3 prime	probably benign
R5660:Smg1	UTSW	7	118,143,347 (GRCm38)	missense	probably benign	0.33
R5706:Smg1	UTSW	7	118,145,590 (GRCm38)	missense	possibly damaging	0.86
R5786:Smg1	UTSW	7	118,212,897 (GRCm38)	missense	probably benign	0.12
R5890:Smg1	UTSW	7	118,190,586 (GRCm38)	utr 3 prime	probably benign
R5912:Smg1	UTSW	7	118,154,586 (GRCm38)	utr 3 prime	probably benign
R5977:Smg1	UTSW	7	118,141,357 (GRCm38)	utr 3 prime	probably benign
R5993:Smg1	UTSW	7	118,140,509 (GRCm38)	missense	probably benign	0.33
R6161:Smg1	UTSW	7	118,163,330 (GRCm38)	utr 3 prime	probably benign
R6187:Smg1	UTSW	7	118,189,163 (GRCm38)	utr 3 prime	probably benign
R6264:Smg1	UTSW	7	118,166,087 (GRCm38)	utr 3 prime	probably benign
R6331:Smg1	UTSW	7	118,154,277 (GRCm38)	utr 3 prime	probably benign
R6561:Smg1	UTSW	7	118,166,077 (GRCm38)	utr 3 prime	probably benign
R6571:Smg1	UTSW	7	118,184,514 (GRCm38)	utr 3 prime	probably benign
R6736:Smg1	UTSW	7	118,157,166 (GRCm38)	utr 3 prime	probably benign
R6752:Smg1	UTSW	7	118,163,316 (GRCm38)	utr 3 prime	probably benign
R6777:Smg1	UTSW	7	118,189,117 (GRCm38)	utr 3 prime	probably benign
R6788:Smg1	UTSW	7	118,184,571 (GRCm38)	utr 3 prime	probably benign
R6883:Smg1	UTSW	7	118,168,180 (GRCm38)	utr 3 prime	probably benign
R6991:Smg1	UTSW	7	118,167,868 (GRCm38)	utr 3 prime	probably benign
R7056:Smg1	UTSW	7	118,146,400 (GRCm38)	splice site	probably benign
R7058:Smg1	UTSW	7	118,198,279 (GRCm38)	utr 3 prime	probably benign
R7100:Smg1	UTSW	7	118,184,520 (GRCm38)	missense	unknown
R7133:Smg1	UTSW	7	118,152,908 (GRCm38)	missense	unknown
R7221:Smg1	UTSW	7	118,182,797 (GRCm38)	missense	possibly damaging	0.86
R7229:Smg1	UTSW	7	118,176,955 (GRCm38)	missense	probably benign	0.03
R7293:Smg1	UTSW	7	118,166,099 (GRCm38)	missense	unknown
R7361:Smg1	UTSW	7	118,184,977 (GRCm38)	missense	unknown
R7438:Smg1	UTSW	7	118,195,893 (GRCm38)	missense	unknown
R7686:Smg1	UTSW	7	118,167,858 (GRCm38)	missense	unknown
R7798:Smg1	UTSW	7	118,171,939 (GRCm38)	missense	possibly damaging	0.73
R7908:Smg1	UTSW	7	118,186,134 (GRCm38)	missense	unknown
R7923:Smg1	UTSW	7	118,143,322 (GRCm38)	missense	possibly damaging	0.96
R7978:Smg1	UTSW	7	118,193,655 (GRCm38)	missense	unknown
R7997:Smg1	UTSW	7	118,173,142 (GRCm38)	missense	unknown
R7997:Smg1	UTSW	7	118,173,141 (GRCm38)	missense	unknown
R8025:Smg1	UTSW	7	118,206,989 (GRCm38)	nonsense	probably null
R8056:Smg1	UTSW	7	118,160,366 (GRCm38)	missense	unknown
R8061:Smg1	UTSW	7	118,152,387 (GRCm38)	missense	unknown
R8095:Smg1	UTSW	7	118,173,062 (GRCm38)	missense	unknown
R8198:Smg1	UTSW	7	118,145,606 (GRCm38)	missense	probably benign	0.03
R8399:Smg1	UTSW	7	118,190,571 (GRCm38)	missense	unknown
R8445:Smg1	UTSW	7	118,136,977 (GRCm38)	missense	possibly damaging	0.72
R8519:Smg1	UTSW	7	118,171,759 (GRCm38)	utr 3 prime	probably benign
R8817:Smg1	UTSW	7	118,159,664 (GRCm38)	missense	unknown
R8832:Smg1	UTSW	7	118,139,783 (GRCm38)	missense	probably benign	0.33
R8855:Smg1	UTSW	7	118,206,899 (GRCm38)	missense	unknown
R8866:Smg1	UTSW	7	118,206,899 (GRCm38)	missense	unknown
R8946:Smg1	UTSW	7	118,152,677 (GRCm38)	missense	probably null
R8954:Smg1	UTSW	7	118,206,992 (GRCm38)	missense	probably damaging	1.00
R8967:Smg1	UTSW	7	118,166,516 (GRCm38)	missense	unknown
R9072:Smg1	UTSW	7	118,183,809 (GRCm38)	missense	unknown
R9090:Smg1	UTSW	7	118,212,563 (GRCm38)	missense	unknown
R9156:Smg1	UTSW	7	118,154,661 (GRCm38)	missense	unknown
R9198:Smg1	UTSW	7	118,195,956 (GRCm38)	missense	unknown
R9240:Smg1	UTSW	7	118,139,808 (GRCm38)	missense	probably benign	0.18
R9271:Smg1	UTSW	7	118,212,563 (GRCm38)	missense	unknown
R9289:Smg1	UTSW	7	118,145,416 (GRCm38)	missense	possibly damaging	0.53
R9378:Smg1	UTSW	7	118,178,775 (GRCm38)	nonsense	probably null
R9396:Smg1	UTSW	7	118,208,080 (GRCm38)	missense	unknown
R9469:Smg1	UTSW	7	118,140,551 (GRCm38)	missense	possibly damaging	0.72
R9539:Smg1	UTSW	7	118,145,753 (GRCm38)	missense	probably benign	0.03
R9549:Smg1	UTSW	7	118,196,031 (GRCm38)	missense	unknown
R9563:Smg1	UTSW	7	118,212,985 (GRCm38)	missense	unknown
R9564:Smg1	UTSW	7	118,212,985 (GRCm38)	missense	unknown
R9597:Smg1	UTSW	7	118,213,047 (GRCm38)	missense	unknown
R9643:Smg1	UTSW	7	118,156,710 (GRCm38)	missense	unknown
R9703:Smg1	UTSW	7	118,140,521 (GRCm38)	missense	possibly damaging	0.73
R9730:Smg1	UTSW	7	118,183,781 (GRCm38)	missense	unknown
Z1088:Smg1	UTSW	7	118,178,399 (GRCm38)	missense	possibly damaging	0.96
Z1088:Smg1	UTSW	7	118,168,661 (GRCm38)	nonsense	probably null
Z1088:Smg1	UTSW	7	118,154,635 (GRCm38)	utr 3 prime	probably benign
Z1176:Smg1	UTSW	7	118,206,907 (GRCm38)	missense	unknown
Z1176:Smg1	UTSW	7	118,206,887 (GRCm38)	missense	unknown
Z1177:Smg1	UTSW	7	118,213,033 (GRCm38)	missense	unknown
Z1177:Smg1	UTSW	7	118,168,608 (GRCm38)	missense	probably null

Predicted Primers

PCR Primer
(F):5'- TCTAGGAGACAAGAGCCTCTTTTG -3'
(R):5'- CCTAAACATTGGCTTCTGGAAAGG -3'

Sequencing Primer
(F):5'- CAAGAGCCTCTTTTGAGTAGATTCC -3'
(R):5'- GAGCTGATGCGCTATGAATG -3'

Posted On

2016-05-10