Mutagenetix > Incidental Mutation

Incidental Mutation 'R5059:Micall1'

386532

Institutional Source

Beutler Lab

Gene Symbol

Micall1

Ensembl Gene

ENSMUSG00000033039

Gene Name

microtubule associated monooxygenase, calponin and LIM domain containing -like 1

Synonyms

Mus EST 820961, D15Mit260, D15N2e

MMRRC Submission

042649-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R5059 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

78993098-79021100 bp(+) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

A to T at 79007034 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000154886 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040320] [ENSMUST00000229031]

AlphaFold

Q8BGT6

Predicted Effect

unknown
Transcript: ENSMUST00000040320
AA Change: Q287L

SMART Domains

Protein: ENSMUSP00000042053
Gene: ENSMUSG00000033039
AA Change: Q287L

Domain	Start	End	E-Value	Type
CH	4	103	5.64e-19	SMART
low complexity region	113	135	N/A	INTRINSIC
LIM	164	219	1.15e-5	SMART
low complexity region	241	250	N/A	INTRINSIC
low complexity region	375	394	N/A	INTRINSIC
low complexity region	414	467	N/A	INTRINSIC
low complexity region	477	497	N/A	INTRINSIC
low complexity region	515	530	N/A	INTRINSIC
low complexity region	571	586	N/A	INTRINSIC
DUF3585	685	825	5.07e-68	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000229004

Predicted Effect

probably benign
Transcript: ENSMUST00000229031

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000229113

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000229694

Meta Mutation Damage Score

0.0645

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 93.9%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam26b	T	A	8: 43,973,637 (GRCm39)	N455I	probably damaging	Het
Alg12	A	G	15: 88,695,659 (GRCm39)	F279S	probably damaging	Het
Angpt4	A	G	2: 151,776,360 (GRCm39)	D296G	probably damaging	Het
Ank1	T	A	8: 23,586,204 (GRCm39)	S437T	probably damaging	Het
Apol7a	G	A	15: 77,274,012 (GRCm39)		probably benign	Het
Arhgef38	T	A	3: 132,843,175 (GRCm39)	Y465F	probably damaging	Het
Atp8a2	T	G	14: 59,928,986 (GRCm39)	T1023P	probably benign	Het
Cdh20	C	A	1: 109,993,430 (GRCm39)	A295E	probably damaging	Het
Cela3b	T	C	4: 137,152,181 (GRCm39)	E92G	probably benign	Het
Cntnap5a	T	C	1: 116,356,224 (GRCm39)	S752P	probably benign	Het
Dlx2	T	C	2: 71,376,585 (GRCm39)	H51R	probably damaging	Het
Dnm2	T	C	9: 21,415,874 (GRCm39)	I736T	probably damaging	Het
Dst	A	G	1: 34,202,427 (GRCm39)	T252A	possibly damaging	Het
Fbxo40	T	C	16: 36,790,658 (GRCm39)	R151G	possibly damaging	Het
Gapdhs	C	T	7: 30,431,410 (GRCm39)	A357T	probably benign	Het
Gon4l	T	A	3: 88,807,319 (GRCm39)	M1671K	probably benign	Het
Gpi1	T	C	7: 33,907,113 (GRCm39)	Y327C	probably damaging	Het
Gucy2g	T	C	19: 55,214,503 (GRCm39)	R542G	probably benign	Het
Hectd3	A	G	4: 116,854,361 (GRCm39)	K308E	possibly damaging	Het
Hip1	A	G	5: 135,478,675 (GRCm39)	F178S	probably damaging	Het
Hydin	A	G	8: 111,232,401 (GRCm39)	D1640G	probably damaging	Het
Il18r1	G	A	1: 40,520,227 (GRCm39)		probably null	Het
Kdm3b	T	C	18: 34,910,250 (GRCm39)	S23P	possibly damaging	Het
Lactb	T	C	9: 66,882,426 (GRCm39)	E83G	probably benign	Het
Larp4	A	T	15: 99,903,171 (GRCm39)	D414V	probably damaging	Het
Map2k5	T	C	9: 63,164,296 (GRCm39)	H336R	probably benign	Het
Mast4	T	C	13: 102,887,071 (GRCm39)	E1006G	probably damaging	Het
Mbd5	T	C	2: 49,146,467 (GRCm39)	S226P	probably damaging	Het
Mtpap	T	A	18: 4,375,827 (GRCm39)		probably benign	Het
Or52ae7	A	T	7: 103,119,488 (GRCm39)	K81*	probably null	Het
Or5p58	A	G	7: 107,694,522 (GRCm39)	L85P	probably damaging	Het
Pnrc1	G	A	4: 33,246,072 (GRCm39)	Q296*	probably null	Het
Poc1a	G	T	9: 106,227,012 (GRCm39)		probably benign	Het
Pramel28	A	G	4: 143,691,565 (GRCm39)	V386A	probably damaging	Het
Prkcsh	T	A	9: 21,924,046 (GRCm39)	L439Q	probably damaging	Het
Prkdc	T	C	16: 15,655,882 (GRCm39)	L3970P	probably damaging	Het
Ptcd1	G	A	5: 145,089,034 (GRCm39)	P542L	probably benign	Het
Rps6ka5	G	A	12: 100,520,634 (GRCm39)	T631I	probably damaging	Het
Rxfp1	T	C	3: 79,570,619 (GRCm39)	N271S	probably benign	Het
Taf6	A	G	5: 138,177,709 (GRCm39)	M541T	probably benign	Het
Tek	T	A	4: 94,692,551 (GRCm39)	C169S	probably benign	Het
Tgfbr3l	T	A	8: 4,299,343 (GRCm39)		probably null	Het
Trpv3	A	G	11: 73,186,149 (GRCm39)	N647D	probably benign	Het
Ube3c	A	G	5: 29,836,293 (GRCm39)	K638R	probably null	Het
Vmn1r191	G	A	13: 22,363,163 (GRCm39)	A197V	probably damaging	Het
Vmn2r6	T	C	3: 64,445,044 (GRCm39)	T894A	possibly damaging	Het
Vstm4	T	G	14: 32,585,687 (GRCm39)	Y85D	probably damaging	Het
Wnt7a	A	G	6: 91,371,482 (GRCm39)	I160T	probably benign	Het
Zc3h7a	TAGAGAG	TAGAGAGAG	16: 10,978,985 (GRCm39)		probably null	Het
Zfp988	A	T	4: 147,416,372 (GRCm39)	K269*	probably null	Het

Other mutations in Micall1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Micall1	APN	15	78,999,221 (GRCm39)	splice site	probably benign
IGL00496:Micall1	APN	15	78,999,221 (GRCm39)	splice site	probably benign
IGL00508:Micall1	APN	15	79,014,768 (GRCm39)	missense	probably damaging	0.99
IGL01626:Micall1	APN	15	79,014,712 (GRCm39)	missense	possibly damaging	0.46
IGL01868:Micall1	APN	15	78,999,260 (GRCm39)	missense	probably benign	0.41
IGL03062:Micall1	APN	15	78,998,881 (GRCm39)	missense	probably damaging	1.00
R0086:Micall1	UTSW	15	79,009,689 (GRCm39)	utr 3 prime	probably benign
R0099:Micall1	UTSW	15	79,016,101 (GRCm39)	splice site	probably benign
R0282:Micall1	UTSW	15	79,016,101 (GRCm39)	splice site	probably benign
R0727:Micall1	UTSW	15	79,004,978 (GRCm39)	missense	probably benign	0.00
R1859:Micall1	UTSW	15	79,007,145 (GRCm39)	intron	probably benign
R2142:Micall1	UTSW	15	79,014,995 (GRCm39)	missense	probably damaging	0.98
R2228:Micall1	UTSW	15	79,014,036 (GRCm39)	missense	probably damaging	1.00
R3508:Micall1	UTSW	15	79,006,965 (GRCm39)	missense	probably damaging	1.00
R4858:Micall1	UTSW	15	79,007,146 (GRCm39)	intron	probably benign
R4888:Micall1	UTSW	15	79,016,048 (GRCm39)	nonsense	probably null
R5097:Micall1	UTSW	15	79,014,078 (GRCm39)	missense	probably benign	0.17
R5451:Micall1	UTSW	15	79,011,104 (GRCm39)	splice site	probably null
R5710:Micall1	UTSW	15	79,011,290 (GRCm39)	missense	probably damaging	1.00
R5727:Micall1	UTSW	15	79,014,678 (GRCm39)	missense	possibly damaging	0.64
R7135:Micall1	UTSW	15	78,993,624 (GRCm39)	missense	unknown
R7186:Micall1	UTSW	15	79,009,575 (GRCm39)	missense	unknown
R7297:Micall1	UTSW	15	79,005,097 (GRCm39)	missense	unknown
R7472:Micall1	UTSW	15	79,006,760 (GRCm39)	missense	unknown
R8494:Micall1	UTSW	15	79,005,080 (GRCm39)	missense	probably damaging	1.00
R8714:Micall1	UTSW	15	79,011,510 (GRCm39)	missense	probably benign	0.03
R8937:Micall1	UTSW	15	79,011,198 (GRCm39)	missense	probably damaging	1.00
R9440:Micall1	UTSW	15	79,011,159 (GRCm39)	missense
R9760:Micall1	UTSW	15	79,005,032 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CAGGAACTAGGCTCCTTTCAC -3'
(R):5'- GGCTATTTACATGCCAACGAG -3'

Sequencing Primer
(F):5'- TAGGCTCCTTTCACAGCAAAGG -3'
(R):5'- CTATTTACATGCCAACGAGAGAGGAC -3'

Posted On

2016-06-06