Incidental Mutation 'R0426:Galr2'
ID 38670
Institutional Source Beutler Lab
Gene Symbol Galr2
Ensembl Gene ENSMUSG00000020793
Gene Name galanin receptor 2
Synonyms mGalR, GalR2
MMRRC Submission 038628-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.077) question?
Stock # R0426 (G1)
Quality Score 168
Status Validated
Chromosome 11
Chromosomal Location 116280939-116283938 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) C to A at 116281691 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Alanine to Aspartic acid at position 69 (A69D)
Ref Sequence ENSEMBL: ENSMUSP00000054062 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000055872]
AlphaFold O88854
Predicted Effect probably damaging
Transcript: ENSMUST00000055872
AA Change: A69D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000054062
Gene: ENSMUSG00000020793
AA Change: A69D

Pfam:7TM_GPCR_Srsx 35 306 4.1e-12 PFAM
Pfam:7tm_1 41 291 6.4e-52 PFAM
Pfam:7TM_GPCR_Srv 62 307 1.2e-7 PFAM
Pfam:7TM_GPCR_Srw 184 308 4.7e-8 PFAM
low complexity region 347 358 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000154277
Meta Mutation Damage Score 0.7092 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 93.9%
Validation Efficiency 96% (86/90)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene display a reduction in exploratory activity. There is also a modest shift in the distribution of different lymphocyte cell types. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 87 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A830010M20Rik C T 5: 107,510,373 (GRCm38) T1603I probably damaging Het
Abca8b G T 11: 109,955,027 (GRCm38) probably benign Het
Acadl A T 1: 66,841,646 (GRCm38) F320L probably damaging Het
Acsbg1 T C 9: 54,622,746 (GRCm38) D222G probably benign Het
Anapc15 A G 7: 101,898,033 (GRCm38) T39A probably benign Het
Ano3 A T 2: 110,661,174 (GRCm38) V919E probably damaging Het
Arhgef12 T C 9: 42,970,990 (GRCm38) probably null Het
Atad5 T A 11: 80,112,832 (GRCm38) I1091N probably benign Het
Atf1 A T 15: 100,232,827 (GRCm38) H26L possibly damaging Het
Atp10a T C 7: 58,784,734 (GRCm38) M252T probably benign Het
Cd55 C T 1: 130,448,372 (GRCm38) R347H probably benign Het
Cdc27 A C 11: 104,513,027 (GRCm38) probably null Het
Cdh9 G A 15: 16,823,454 (GRCm38) probably null Het
Cdk11b T C 4: 155,642,512 (GRCm38) probably benign Het
Cep70 A G 9: 99,297,684 (GRCm38) D567G probably benign Het
Cep78 A T 19: 15,970,970 (GRCm38) Y382* probably null Het
Col9a2 T C 4: 121,044,660 (GRCm38) probably benign Het
Cyp2d12 G A 15: 82,558,963 (GRCm38) D409N probably benign Het
Ddx39 A G 8: 83,721,769 (GRCm38) T217A probably benign Het
Dennd1b T A 1: 139,170,196 (GRCm38) D733E probably benign Het
Dicer1 A G 12: 104,702,542 (GRCm38) S1294P probably damaging Het
Dnah3 T C 7: 119,943,572 (GRCm38) E3539G probably benign Het
Dnmbp A G 19: 43,852,436 (GRCm38) probably benign Het
Dysf T C 6: 84,149,757 (GRCm38) L1332P probably damaging Het
F5 A G 1: 164,182,840 (GRCm38) D380G probably damaging Het
Fam160a2 A C 7: 105,389,473 (GRCm38) C186W probably damaging Het
Fam171a1 T C 2: 3,225,396 (GRCm38) V522A probably benign Het
Grk2 T C 19: 4,290,600 (GRCm38) probably null Het
Gtf3c1 A T 7: 125,663,016 (GRCm38) Y1119* probably null Het
Hgd A T 16: 37,588,685 (GRCm38) probably benign Het
Ildr2 G T 1: 166,308,899 (GRCm38) V436L probably benign Het
Intu G A 3: 40,675,305 (GRCm38) C355Y probably damaging Het
Irf2bpl G T 12: 86,883,096 (GRCm38) P268T probably benign Het
Jarid2 T C 13: 44,840,882 (GRCm38) probably null Het
Jup A T 11: 100,372,401 (GRCm38) M716K probably benign Het
Kank1 G A 19: 25,411,473 (GRCm38) V809I probably damaging Het
Kdm1b T A 13: 47,064,244 (GRCm38) probably benign Het
Kdm3a C T 6: 71,600,755 (GRCm38) C687Y probably damaging Het
Kdm5d T A Y: 942,437 (GRCm38) probably benign Het
Kifap3 T A 1: 163,865,552 (GRCm38) probably benign Het
Macf1 T A 4: 123,483,660 (GRCm38) K1400* probably null Het
Majin A G 19: 6,212,117 (GRCm38) probably benign Het
Mb21d1 G A 9: 78,435,738 (GRCm38) probably benign Het
Mctp1 A G 13: 77,020,821 (GRCm38) I846V probably benign Het
Mrgpra2b T A 7: 47,464,127 (GRCm38) I286F possibly damaging Het
Neil3 T G 8: 53,609,396 (GRCm38) probably benign Het
Nox3 G T 17: 3,695,563 (GRCm38) N23K probably damaging Het
Nt5c3 T C 6: 56,883,812 (GRCm38) K219E probably benign Het
Olfr1458 G A 19: 13,103,278 (GRCm38) R3C possibly damaging Het
Olfr213 A T 6: 116,540,485 (GRCm38) N11Y probably damaging Het
Olfr389 T A 11: 73,776,437 (GRCm38) M297L probably benign Het
Olfr524 A C 7: 140,202,116 (GRCm38) F218C possibly damaging Het
Olfr548-ps1 T A 7: 102,542,686 (GRCm38) I250N probably damaging Het
Olfr954 T C 9: 39,461,593 (GRCm38) L54P probably damaging Het
Pacsin2 A G 15: 83,379,795 (GRCm38) V347A possibly damaging Het
Pcdhb7 A T 18: 37,342,804 (GRCm38) E331V probably damaging Het
Pcid2 A C 8: 13,081,262 (GRCm38) probably null Het
Pcsk9 T C 4: 106,450,077 (GRCm38) D323G possibly damaging Het
Pdhb T C 14: 8,169,801 (GRCm38) E203G probably damaging Het
Phlpp2 A G 8: 109,928,463 (GRCm38) Y630C probably benign Het
Pidd1 C T 7: 141,439,133 (GRCm38) A812T probably damaging Het
Plau G A 14: 20,842,314 (GRCm38) R389H probably benign Het
Plekhg6 G A 6: 125,364,629 (GRCm38) probably null Het
Ppox T C 1: 171,277,749 (GRCm38) Y321C probably damaging Het
Pxdn A G 12: 29,987,066 (GRCm38) N281S possibly damaging Het
Pycrl A T 15: 75,918,388 (GRCm38) M138K probably benign Het
Radil T C 5: 142,497,873 (GRCm38) Y526C probably damaging Het
Ranbp3 C A 17: 56,707,169 (GRCm38) D233E probably benign Het
Rhpn1 A G 15: 75,711,872 (GRCm38) Q402R possibly damaging Het
Sec23b T A 2: 144,568,612 (GRCm38) probably benign Het
Sel1l2 A T 2: 140,240,912 (GRCm38) L602* probably null Het
Sema5b G A 16: 35,646,355 (GRCm38) G209D probably damaging Het
Svep1 T C 4: 58,073,333 (GRCm38) Y1992C possibly damaging Het
Syncrip T A 9: 88,456,259 (GRCm38) probably benign Het
Synj1 G T 16: 90,967,354 (GRCm38) A65E probably damaging Het
Taar2 G A 10: 23,941,495 (GRCm38) R311H probably benign Het
Tecrl T C 5: 83,354,763 (GRCm38) probably benign Het
Tenm4 G T 7: 96,777,851 (GRCm38) G698C probably damaging Het
Tmem209 G A 6: 30,491,182 (GRCm38) L259F probably damaging Het
Tmem247 G A 17: 86,918,503 (GRCm38) E124K possibly damaging Het
Tnks2 C A 19: 36,852,821 (GRCm38) A218E probably damaging Het
Tppp T A 13: 74,021,311 (GRCm38) F57I probably damaging Het
Trim36 A G 18: 46,172,525 (GRCm38) W452R probably damaging Het
Vars2 A T 17: 35,664,584 (GRCm38) V262E probably damaging Het
Vmn2r92 C T 17: 18,167,957 (GRCm38) A408V probably damaging Het
Zfp516 G T 18: 82,955,772 (GRCm38) A32S probably benign Het
Zfy2 G T Y: 2,107,348 (GRCm38) L429I possibly damaging Het
Other mutations in Galr2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01012:Galr2 APN 11 116,283,170 (GRCm38) missense probably damaging 1.00
PIT4418001:Galr2 UTSW 11 116,283,258 (GRCm38) missense probably benign 0.35
PIT4445001:Galr2 UTSW 11 116,281,648 (GRCm38) missense probably benign 0.13
R1869:Galr2 UTSW 11 116,283,243 (GRCm38) missense possibly damaging 0.87
R2059:Galr2 UTSW 11 116,282,939 (GRCm38) missense probably damaging 1.00
R4579:Galr2 UTSW 11 116,281,499 (GRCm38) missense probably benign
R4666:Galr2 UTSW 11 116,283,629 (GRCm38) missense probably benign
R5832:Galr2 UTSW 11 116,281,631 (GRCm38) missense probably damaging 1.00
R5974:Galr2 UTSW 11 116,283,026 (GRCm38) missense possibly damaging 0.62
R7081:Galr2 UTSW 11 116,283,048 (GRCm38) missense probably damaging 0.99
R7155:Galr2 UTSW 11 116,283,582 (GRCm38) missense possibly damaging 0.94
R7696:Galr2 UTSW 11 116,283,167 (GRCm38) missense probably damaging 1.00
R7810:Galr2 UTSW 11 116,283,120 (GRCm38) missense probably benign 0.23
R8921:Galr2 UTSW 11 116,283,147 (GRCm38) missense probably damaging 1.00
R9231:Galr2 UTSW 11 116,283,509 (GRCm38) missense probably benign
R9514:Galr2 UTSW 11 116,283,626 (GRCm38) missense probably benign
X0009:Galr2 UTSW 11 116,283,323 (GRCm38) missense probably benign 0.14
X0026:Galr2 UTSW 11 116,281,751 (GRCm38) missense probably benign
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2013-05-23