Mutagenetix > Incidental Mutation

Incidental Mutation 'R5078:Or5d20-ps1'

386949

Institutional Source

Beutler Lab

Gene Symbol

Or5d20-ps1

Ensembl Gene

ENSMUSG00000046590

Gene Name

olfactory receptor family 5 subfamily D member 20, pseudogene 1

Synonyms

MOR174-7, Olfr1165-ps, GA_x6K02T2Q125-49593880-49592930

MMRRC Submission

042667-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R5078 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

87931380-87932329 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 87931879 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 151 (I151L)

Ref Sequence

ENSEMBL: ENSMUSP00000149054 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000117860]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000117860
AA Change: I151L

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy9	T	C	16: 4,141,771 (GRCm39)	S345G	probably benign	Het
Adgrf2	A	T	17: 43,021,877 (GRCm39)	F316I	probably damaging	Het
Ank2	A	G	3: 126,736,002 (GRCm39)		probably benign	Het
Auts2	T	C	5: 132,287,786 (GRCm39)	K66E	possibly damaging	Het
Bicra	T	C	7: 15,709,382 (GRCm39)	D1144G	probably damaging	Het
Cabin1	A	G	10: 75,557,312 (GRCm39)	S1109P	probably damaging	Het
Camkv	T	C	9: 107,822,572 (GRCm39)	V29A	probably damaging	Het
Ccdc178	A	T	18: 22,200,685 (GRCm39)		probably null	Het
Ccser1	G	A	6: 61,288,350 (GRCm39)	R171H	probably damaging	Het
Cdk19	A	G	10: 40,312,150 (GRCm39)	Y133C	probably damaging	Het
Cerkl	T	A	2: 79,223,352 (GRCm39)	D123V	probably benign	Het
Chd1	T	C	17: 15,946,616 (GRCm39)	S121P	possibly damaging	Het
Col4a2	T	C	8: 11,493,936 (GRCm39)	V1459A	probably benign	Het
Dlg1	T	A	16: 31,675,287 (GRCm39)	Y704*	probably null	Het
Dsg2	T	C	18: 20,729,140 (GRCm39)		probably null	Het
Egln3	T	C	12: 54,228,453 (GRCm39)	R218G	probably damaging	Het
Gm11568	T	C	11: 99,749,181 (GRCm39)	C129R	unknown	Het
Gm13991	T	C	2: 116,358,355 (GRCm39)		noncoding transcript	Het
Gmnc	C	A	16: 26,784,332 (GRCm39)	V58L	probably benign	Het
Gpx5	A	G	13: 21,472,881 (GRCm39)	F151S	probably damaging	Het
Helz	G	A	11: 107,546,922 (GRCm39)	G1079R	probably damaging	Het
Ice1	T	C	13: 70,752,969 (GRCm39)	E1039G	probably benign	Het
Kif18a	T	C	2: 109,125,487 (GRCm39)		probably benign	Het
Klhl29	T	C	12: 5,143,530 (GRCm39)	T500A	possibly damaging	Het
Lrfn5	A	C	12: 61,890,660 (GRCm39)	K650Q	possibly damaging	Het
Lrp2	T	C	2: 69,331,874 (GRCm39)	D1627G	possibly damaging	Het
Nxn	T	C	11: 76,152,433 (GRCm39)	K354E	probably damaging	Het
Pcnx2	T	C	8: 126,478,895 (GRCm39)	T2118A	probably benign	Het
Phldb2	A	G	16: 45,598,105 (GRCm39)	F861L	possibly damaging	Het
Piezo2	A	G	18: 63,157,607 (GRCm39)	Y2368H	probably damaging	Het
Pnp	T	A	14: 51,188,963 (GRCm39)	L252*	probably null	Het
Prom2	T	C	2: 127,373,757 (GRCm39)	Q641R	probably benign	Het
Prpf31	T	C	7: 3,637,702 (GRCm39)	S180P	possibly damaging	Het
Prss27	C	A	17: 24,263,414 (GRCm39)	Y142*	probably null	Het
Ripk1	T	G	13: 34,201,082 (GRCm39)	M265R	probably damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Ropn1	T	A	16: 34,487,161 (GRCm39)	D32E	probably damaging	Het
Rps23	T	C	13: 91,071,822 (GRCm39)	F41L	probably benign	Het
Sec24d	A	G	3: 123,084,201 (GRCm39)	I127V	probably benign	Het
Selenot	C	T	3: 58,492,692 (GRCm39)	R60W	probably damaging	Het
Tll1	T	C	8: 64,546,921 (GRCm39)	K342E	probably damaging	Het
Tlx1	A	T	19: 45,144,460 (GRCm39)	N61Y	probably damaging	Het
Vmn2r117	T	A	17: 23,679,122 (GRCm39)	I701F	probably damaging	Het
Zmynd12	A	G	4: 119,302,047 (GRCm39)	K229E	probably damaging	Het

Other mutations in Or5d20-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R4378:Or5d20-ps1	UTSW	2	87,931,829 (GRCm39)	missense	unknown
R6373:Or5d20-ps1	UTSW	2	87,932,189 (GRCm39)	missense	possibly damaging	0.73
R6698:Or5d20-ps1	UTSW	2	87,931,561 (GRCm39)	missense	unknown
R6736:Or5d20-ps1	UTSW	2	87,931,947 (GRCm39)	missense	probably benign	0.03
R7164:Or5d20-ps1	UTSW	2	87,932,176 (GRCm39)	missense	probably damaging	0.97
R7285:Or5d20-ps1	UTSW	2	87,932,049 (GRCm39)	missense	probably damaging	1.00
R7807:Or5d20-ps1	UTSW	2	87,931,909 (GRCm39)	missense	probably benign	0.00
R8040:Or5d20-ps1	UTSW	2	87,931,703 (GRCm39)	missense	unknown
R8509:Or5d20-ps1	UTSW	2	87,931,866 (GRCm39)	missense	probably benign	0.16
R9047:Or5d20-ps1	UTSW	2	87,931,416 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGGAGTCCACCCTTAGAAGG -3'
(R):5'- GGAGGACAGGACAATCTCTTTC -3'

Sequencing Primer
(F):5'- GTCCACCCTTAGAAGGCATCTTAATG -3'
(R):5'- CAGGACAATCTCTTTCAAAGGATGC -3'

Posted On

2016-06-06