Incidental Mutation 'R5083:Gm14409'
ID387232
Institutional Source Beutler Lab
Gene Symbol Gm14409
Ensembl Gene ENSMUSG00000078869
Gene Namepredicted gene 14409
Synonyms
MMRRC Submission 042672-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.061) question?
Stock #R5083 (G1)
Quality Score180
Status Not validated
Chromosome2
Chromosomal Location177264654-177267036 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 177265571 bp
ZygosityHeterozygous
Amino Acid Change Phenylalanine to Leucine at position 45 (F45L)
Ref Sequence ENSEMBL: ENSMUSP00000104591 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000108963]
Predicted Effect probably damaging
Transcript: ENSMUST00000108963
AA Change: F45L

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000104591
Gene: ENSMUSG00000078869
AA Change: F45L

DomainStartEndE-ValueType
Blast:KRAB 1 34 3e-14 BLAST
ZnF_C2H2 46 65 1.31e2 SMART
ZnF_C2H2 71 93 8.47e-4 SMART
ZnF_C2H2 99 121 5.99e-4 SMART
ZnF_C2H2 127 149 5.59e-4 SMART
ZnF_C2H2 155 177 2.53e-2 SMART
ZnF_C2H2 183 205 1.72e-4 SMART
ZnF_C2H2 211 233 2.12e-4 SMART
ZnF_C2H2 239 261 1.58e-3 SMART
ZnF_C2H2 267 289 1.03e-2 SMART
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 95.0%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A530064D06Rik C T 17: 48,166,390 V120M possibly damaging Het
Abca6 T C 11: 110,218,967 D646G probably damaging Het
Agbl5 G A 5: 30,903,059 R141Q probably damaging Het
Arid1b A G 17: 5,314,018 T554A possibly damaging Het
Atp2a3 G T 11: 72,982,826 V824L probably null Het
Bet1 T C 6: 4,077,895 I115V possibly damaging Het
Cdc23 C A 18: 34,651,689 V7L unknown Het
Cfap65 A G 1: 74,906,441 S1373P probably damaging Het
Chd9 T C 8: 90,984,374 L353P probably damaging Het
Chil3 C A 3: 106,164,089 probably null Het
Comp C T 8: 70,381,300 T655M probably damaging Het
Dctd T C 8: 48,111,716 Y18H probably damaging Het
Ddx39b G A 17: 35,253,029 G348D possibly damaging Het
Dhx36 A T 3: 62,471,999 S889R probably benign Het
Dtx2 T C 5: 136,012,190 Y150H probably damaging Het
Epx A G 11: 87,872,680 F238S probably damaging Het
Ergic2 T C 6: 148,196,014 T154A probably benign Het
Esco1 A G 18: 10,594,734 I184T probably benign Het
Esf1 G T 2: 140,157,071 A495E possibly damaging Het
Esf1 T C 2: 140,158,579 Y429C possibly damaging Het
Fcho1 C A 8: 71,717,176 R101L probably benign Het
Foxn4 T A 5: 114,256,927 D313V probably damaging Het
Gm11568 T C 11: 99,857,972 M1T probably null Het
Gphn T A 12: 78,623,289 probably null Het
Grid2 C T 6: 64,320,152 Q500* probably null Het
Igsf10 A G 3: 59,326,273 S1680P probably damaging Het
Ints12 T C 3: 133,100,777 M155T possibly damaging Het
Invs A T 4: 48,396,307 M327L possibly damaging Het
Kdm3a T C 6: 71,621,362 E180G probably damaging Het
Mgat3 G A 15: 80,211,298 V109M possibly damaging Het
Mrgprb3 A G 7: 48,643,014 V263A probably benign Het
Mroh7 A T 4: 106,690,318 V1109D probably benign Het
Myo15b A T 11: 115,866,656 T1111S probably benign Het
Myo19 G T 11: 84,903,211 A654S possibly damaging Het
Mypn A T 10: 63,118,528 V1224D probably damaging Het
Nalcn A G 14: 123,323,294 probably null Het
Olfr250 G A 9: 38,368,062 C172Y possibly damaging Het
Olfr437 G T 6: 43,167,339 A94S probably benign Het
Olfr676 A T 7: 105,035,411 Y71F probably damaging Het
Pdcd2 A G 17: 15,522,822 I247T possibly damaging Het
Pik3c2a A T 7: 116,342,401 N1571K probably damaging Het
Plagl2 T C 2: 153,236,044 T6A probably benign Het
Ros1 T A 10: 52,163,941 Y318F possibly damaging Het
Sdccag8 C A 1: 176,824,892 H70N probably damaging Het
Skint1 A G 4: 112,029,433 R359G probably benign Het
Slc44a5 A T 3: 154,247,787 I269L probably benign Het
Slfn10-ps T A 11: 83,030,515 noncoding transcript Het
Suclg1 C A 6: 73,263,980 T164K probably benign Het
Tgds C A 14: 118,116,079 probably null Het
Ttn T C 2: 76,813,533 D13117G probably damaging Het
Ttn T C 2: 76,870,737 probably benign Het
Vmn2r28 A T 7: 5,480,672 I843N possibly damaging Het
Vmn2r52 A T 7: 10,159,465 Y582* probably null Het
Vps33b G T 7: 80,274,641 K65N probably damaging Het
Other mutations in Gm14409
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1549:Gm14409 UTSW 2 177265585 missense probably damaging 1.00
R2125:Gm14409 UTSW 2 177265402 missense probably damaging 1.00
R3833:Gm14409 UTSW 2 177265205 missense possibly damaging 0.64
R4438:Gm14409 UTSW 2 177265096 missense possibly damaging 0.90
R4906:Gm14409 UTSW 2 177265430 missense possibly damaging 0.90
R5759:Gm14409 UTSW 2 177264972 nonsense probably null
R5802:Gm14409 UTSW 2 177265256 missense possibly damaging 0.95
R7026:Gm14409 UTSW 2 177265568 missense probably benign 0.02
Predicted Primers PCR Primer
(F):5'- TACATTCATAGGGTTTCTCTCCTGT -3'
(R):5'- GTGAGCCAGATAATCAGTACTGT -3'

Sequencing Primer
(F):5'- GTGCAAAGGCTTTACCACATTG -3'
(R):5'- CAGATAATCAGTACTGTTCCCATTTC -3'
Posted On2016-06-06