Mutagenetix > Incidental Mutation

Incidental Mutation 'R5084:Zfp786'

387299

Institutional Source

Beutler Lab

Gene Symbol

Zfp786

Ensembl Gene

ENSMUSG00000051499

Gene Name

zinc finger protein 786

Synonyms

A730012O14Rik

MMRRC Submission

042673-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R5084 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

47796200-47807801 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 47796953 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 662 (M662V)

Ref Sequence

ENSEMBL: ENSMUSP00000057495 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058844]

AlphaFold

Q8BV42

Predicted Effect

probably benign
Transcript: ENSMUST00000058844
AA Change: M662V

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000057495
Gene: ENSMUSG00000051499
AA Change: M662V

Domain	Start	End	E-Value	Type
KRAB	9	69	2.6e-22	SMART
low complexity region	156	164	N/A	INTRINSIC
ZnF_C2H2	240	262	5.34e-1	SMART
ZnF_C2H2	268	295	1.41e2	SMART
ZnF_C2H2	420	440	1.21e2	SMART
ZnF_C2H2	448	470	3.52e-1	SMART
ZnF_C2H2	476	498	1.89e-1	SMART
ZnF_C2H2	504	526	1.69e-3	SMART
ZnF_C2H2	532	554	5.59e-4	SMART
ZnF_C2H2	560	582	4.79e-3	SMART
ZnF_C2H2	588	610	3.69e-4	SMART
ZnF_C2H2	616	638	3.63e-3	SMART
ZnF_C2H2	644	665	5.92e0	SMART
ZnF_C2H2	671	693	2.57e-3	SMART
ZnF_C2H2	699	721	1.38e-3	SMART
ZnF_C2H2	727	749	1.45e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000080603

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143979

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.3%
20x: 92.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acp3	T	A	9: 104,204,116 (GRCm39)	E59D	probably damaging	Het
Akap7	T	A	10: 25,155,640 (GRCm39)		probably benign	Het
Anpep	C	A	7: 79,476,618 (GRCm39)		probably null	Het
Aste1	T	A	9: 105,274,886 (GRCm39)	Y314*	probably null	Het
Ccdc183	G	A	2: 25,498,802 (GRCm39)	T497I	probably damaging	Het
Cd4	A	T	6: 124,847,402 (GRCm39)	I254N	probably damaging	Het
Crot	G	T	5: 9,019,994 (GRCm39)	H449Q	probably damaging	Het
Dact2	A	T	17: 14,418,214 (GRCm39)	W164R	possibly damaging	Het
Dapk3	C	A	10: 81,026,152 (GRCm39)		probably null	Het
Dnase1l1	C	T	X: 73,320,644 (GRCm39)		probably null	Het
Fbrsl1	G	A	5: 110,527,272 (GRCm39)		probably benign	Het
Flg	T	C	3: 93,184,922 (GRCm39)	F15L	probably damaging	Het
Fscn2	A	G	11: 120,252,686 (GRCm39)	D51G	probably damaging	Het
H2-Ob	G	T	17: 34,460,102 (GRCm39)	G71V	probably damaging	Het
Hmgcs1	T	C	13: 120,161,520 (GRCm39)	V104A	possibly damaging	Het
Hs3st4	G	T	7: 123,996,518 (GRCm39)	D395Y	probably damaging	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Ldlrad3	G	A	2: 101,900,329 (GRCm39)	R58C	probably damaging	Het
Lpin1	A	G	12: 16,626,983 (GRCm39)	S188P	probably damaging	Het
Mamdc2	G	T	19: 23,336,516 (GRCm39)	T331K	possibly damaging	Het
Matr3	G	A	18: 35,715,135 (GRCm39)	S195N	probably damaging	Het
Nktr	T	C	9: 121,577,176 (GRCm39)	Y390H	possibly damaging	Het
Notch3	C	T	17: 32,376,864 (GRCm39)		probably null	Het
Olfm3	T	C	3: 114,698,202 (GRCm39)		probably null	Het
Or10ak11	A	G	4: 118,686,767 (GRCm39)	V290A	probably damaging	Het
Or2a20	T	C	6: 43,194,594 (GRCm39)	L249P	probably damaging	Het
Or5h25	C	T	16: 58,930,436 (GRCm39)	C179Y	possibly damaging	Het
Or7e173	T	A	9: 19,938,551 (GRCm39)	I228F	probably damaging	Het
Or7g17	T	A	9: 18,768,632 (GRCm39)	M237K	probably benign	Het
Pask	A	G	1: 93,249,819 (GRCm39)	V527A	probably benign	Het
Pcdha6	A	T	18: 37,102,016 (GRCm39)	N403I	probably damaging	Het
Peg3	T	C	7: 6,710,848 (GRCm39)	E1458G	probably damaging	Het
Pip4k2b	G	A	11: 97,610,569 (GRCm39)	T386M	probably damaging	Het
Pkd1l1	T	C	11: 8,892,004 (GRCm39)	M272V	probably benign	Het
Plcb4	A	G	2: 135,844,571 (GRCm39)	E163G	probably damaging	Het
Plk5	C	A	10: 80,194,723 (GRCm39)	R149S	possibly damaging	Het
Pou4f3	A	T	18: 42,528,933 (GRCm39)	Y292F	probably damaging	Het
Rasgef1c	A	G	11: 49,860,332 (GRCm39)	K272E	probably damaging	Het
Rbl2	C	T	8: 91,841,759 (GRCm39)	T942M	probably benign	Het
Sipa1l3	G	A	7: 29,048,000 (GRCm39)	S247F	probably damaging	Het
Smarca4	T	C	9: 21,572,059 (GRCm39)	L777P	probably damaging	Het
Smpd1	T	C	7: 105,206,185 (GRCm39)	Y438H	probably damaging	Het
Sorl1	T	C	9: 41,887,673 (GRCm39)	K2052R	probably benign	Het
Spata31d1c	C	A	13: 65,182,944 (GRCm39)	P162Q	probably damaging	Het
Spmip9	A	G	6: 70,892,688 (GRCm39)	S20P	possibly damaging	Het
Ston1	A	T	17: 88,944,002 (GRCm39)	E469D	probably benign	Het
Syde2	AGTTC	AGTTCGTTC	3: 145,707,164 (GRCm39)		probably null	Het
Syde2	CAGTT	CAGTTAGTT	3: 145,707,163 (GRCm39)		probably null	Het
Taar5	T	A	10: 23,846,836 (GRCm39)	L78Q	probably damaging	Het
Tas2r114	A	T	6: 131,666,251 (GRCm39)	L259*	probably null	Het
Topaz1	T	A	9: 122,577,883 (GRCm39)	H264Q	probably benign	Het
Vcl	G	T	14: 21,059,027 (GRCm39)	V548L	possibly damaging	Het
Vmn2r57	C	A	7: 41,075,974 (GRCm39)		probably null	Het
Vmn2r92	A	G	17: 18,405,439 (GRCm39)	*861W	probably null	Het
Ylpm1	T	G	12: 85,076,095 (GRCm39)	V940G	probably damaging	Het
Zfp236	A	G	18: 82,627,556 (GRCm39)	S1500P	probably damaging	Het
Zfp384	G	T	6: 125,000,642 (GRCm39)		probably benign	Het

Other mutations in Zfp786

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Zfp786	APN	6	47,796,605 (GRCm39)	makesense	probably null
IGL02442:Zfp786	APN	6	47,798,301 (GRCm39)	missense	probably benign	0.00
IGL02674:Zfp786	APN	6	47,797,427 (GRCm39)	missense	probably benign	0.22
IGL02814:Zfp786	APN	6	47,796,775 (GRCm39)	missense	probably damaging	1.00
IGL03072:Zfp786	APN	6	47,798,177 (GRCm39)	missense	probably benign	0.01
IGL03294:Zfp786	APN	6	47,798,258 (GRCm39)	nonsense	probably null
IGL03393:Zfp786	APN	6	47,798,458 (GRCm39)	missense	possibly damaging	0.88
3-1:Zfp786	UTSW	6	47,797,379 (GRCm39)	missense	probably damaging	1.00
PIT4581001:Zfp786	UTSW	6	47,796,756 (GRCm39)	missense	probably damaging	1.00
R1071:Zfp786	UTSW	6	47,798,239 (GRCm39)	missense	possibly damaging	0.91
R1426:Zfp786	UTSW	6	47,802,013 (GRCm39)	missense	probably benign	0.17
R1976:Zfp786	UTSW	6	47,796,691 (GRCm39)	missense	probably damaging	1.00
R2114:Zfp786	UTSW	6	47,803,931 (GRCm39)	missense	probably damaging	1.00
R2115:Zfp786	UTSW	6	47,803,931 (GRCm39)	missense	probably damaging	1.00
R2117:Zfp786	UTSW	6	47,803,931 (GRCm39)	missense	probably damaging	1.00
R2176:Zfp786	UTSW	6	47,797,905 (GRCm39)	missense	possibly damaging	0.65
R2304:Zfp786	UTSW	6	47,797,633 (GRCm39)	missense	probably damaging	1.00
R3110:Zfp786	UTSW	6	47,797,160 (GRCm39)	missense	probably damaging	1.00
R3112:Zfp786	UTSW	6	47,797,160 (GRCm39)	missense	probably damaging	1.00
R3623:Zfp786	UTSW	6	47,798,357 (GRCm39)	missense	probably benign	0.00
R4654:Zfp786	UTSW	6	47,797,868 (GRCm39)	missense	probably benign	0.01
R4741:Zfp786	UTSW	6	47,797,625 (GRCm39)	missense	probably damaging	1.00
R4927:Zfp786	UTSW	6	47,797,087 (GRCm39)	missense	probably benign	0.43
R4936:Zfp786	UTSW	6	47,798,202 (GRCm39)	nonsense	probably null
R5445:Zfp786	UTSW	6	47,796,619 (GRCm39)	missense	probably damaging	1.00
R6662:Zfp786	UTSW	6	47,803,920 (GRCm39)	missense	probably damaging	1.00
R7290:Zfp786	UTSW	6	47,796,929 (GRCm39)	missense	probably damaging	1.00
R7561:Zfp786	UTSW	6	47,796,667 (GRCm39)	missense	probably benign	0.02
R8038:Zfp786	UTSW	6	47,798,188 (GRCm39)	missense	probably benign	0.01
R8090:Zfp786	UTSW	6	47,796,943 (GRCm39)	missense	probably damaging	1.00
R8250:Zfp786	UTSW	6	47,797,729 (GRCm39)	missense	possibly damaging	0.85
R8438:Zfp786	UTSW	6	47,796,934 (GRCm39)	missense	probably damaging	1.00
R8516:Zfp786	UTSW	6	47,797,477 (GRCm39)	missense	probably damaging	1.00
R8868:Zfp786	UTSW	6	47,802,015 (GRCm39)	missense	probably damaging	0.99
R9596:Zfp786	UTSW	6	47,797,406 (GRCm39)	nonsense	probably null
R9788:Zfp786	UTSW	6	47,797,816 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GAAGCCCTTTCCACAGTCAC -3'
(R):5'- TAAAGGGGCAGCTGCTTAG -3'

Sequencing Primer
(F):5'- TTTCCACAGTCACCGCAG -3'
(R):5'- AGCTGCTTAGCCATCAGC -3'

Posted On

2016-06-06