Mutagenetix > Incidental Mutation

Incidental Mutation 'R5087:Or2g25'

387539

Institutional Source

Beutler Lab

Gene Symbol

Or2g25

Ensembl Gene

ENSMUSG00000095286

Gene Name

olfactory receptor family 2 subfamily G member 25

Synonyms

MOR256-33, GA_x6K02T2PSCP-2119438-2118485, Olfr117

MMRRC Submission

042676-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R5087 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

37970269-37971222 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 37970612 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 204 (V204E)

Ref Sequence

ENSEMBL: ENSMUSP00000150204 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073636] [ENSMUST00000213638] [ENSMUST00000215414]

AlphaFold

L7N1Z1

Predicted Effect

probably damaging
Transcript: ENSMUST00000073636
AA Change: V204E

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000073320
Gene: ENSMUSG00000095286
AA Change: V204E

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	307	2.7e-44	PFAM
Pfam:7TM_GPCR_Srsx	34	222	2e-5	PFAM
Pfam:7tm_1	40	289	2.9e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213638
AA Change: V204E

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215414
AA Change: V204E

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.1%

Validation Efficiency

99% (66/67)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adhfe1	A	G	1: 9,631,851 (GRCm39)		probably benign	Het
Anln	T	C	9: 22,286,340 (GRCm39)	T322A	possibly damaging	Het
Best3	A	T	10: 116,844,907 (GRCm39)	Y354F	probably benign	Het
Cd44	C	T	2: 102,661,699 (GRCm39)	V509I	possibly damaging	Het
Cdk5rap1	T	C	2: 154,184,315 (GRCm39)	N529S	probably damaging	Het
Cnst	A	G	1: 179,450,378 (GRCm39)	D614G	possibly damaging	Het
Cpq	T	C	15: 33,213,008 (GRCm39)	I9T	probably benign	Het
Cyp2j6	T	C	4: 96,419,936 (GRCm39)	H265R	probably damaging	Het
Dctn1	T	A	6: 83,168,621 (GRCm39)	M458K	probably damaging	Het
Disc1	A	C	8: 125,858,899 (GRCm39)	I454L	probably benign	Het
Dnaja4	A	G	9: 54,607,023 (GRCm39)	E4G	probably damaging	Het
Dnase1l1	C	T	X: 73,320,644 (GRCm39)		probably null	Het
Eipr1	C	A	12: 28,878,855 (GRCm39)	N136K	probably benign	Het
Fut11	A	G	14: 20,746,229 (GRCm39)	N357S	probably damaging	Het
Gbp2b	C	T	3: 142,304,015 (GRCm39)	P37S	probably damaging	Het
Ghr	T	G	15: 3,349,622 (GRCm39)	K519Q	probably damaging	Het
Gipr	C	A	7: 18,893,689 (GRCm39)	C328F	probably damaging	Het
Gm27013	A	T	6: 130,654,633 (GRCm39)	N276K	probably damaging	Het
Got2	C	A	8: 96,598,951 (GRCm39)	K150N	probably benign	Het
Gucy2c	A	G	6: 136,744,033 (GRCm39)	S189P	possibly damaging	Het
H2-T5	C	A	17: 36,476,308 (GRCm39)	G347*	probably null	Het
Hells	T	C	19: 38,932,189 (GRCm39)	V153A	probably benign	Het
Ighd	G	A	12: 113,378,047 (GRCm39)		probably benign	Het
Inhca	C	T	9: 103,143,420 (GRCm39)	V361M	probably damaging	Het
Itga4	A	C	2: 79,145,973 (GRCm39)	D725A	possibly damaging	Het
Itgbl1	T	A	14: 124,204,151 (GRCm39)	L414Q	possibly damaging	Het
Kat2b-ps	A	G	5: 93,539,585 (GRCm39)		noncoding transcript	Het
Lrrc1	A	T	9: 77,364,740 (GRCm39)	S192T	probably benign	Het
Mc2r	A	T	18: 68,541,274 (GRCm39)	N6K	probably benign	Het
Moxd2	A	C	6: 40,856,270 (GRCm39)	L534W	probably damaging	Het
Myh4	A	T	11: 67,146,235 (GRCm39)	D1431V	probably damaging	Het
Naa15	T	A	3: 51,364,706 (GRCm39)		probably null	Het
Naa16	G	A	14: 79,614,855 (GRCm39)	T215I	possibly damaging	Het
Ntng1	A	T	3: 110,042,645 (GRCm39)	Y60*	probably null	Het
Or1e29	T	C	11: 73,668,084 (GRCm39)	Y23C	possibly damaging	Het
Pccb	T	C	9: 100,867,296 (GRCm39)		probably benign	Het
Pcnx1	C	A	12: 82,041,713 (GRCm39)	P2115Q	probably damaging	Het
Pfkfb3	G	A	2: 11,488,825 (GRCm39)	T313I	probably damaging	Het
Pld1	A	G	3: 28,178,731 (GRCm39)	N875S	probably damaging	Het
Ptpn20	A	T	14: 33,336,398 (GRCm39)	D79V	possibly damaging	Het
Rabgap1l	C	A	1: 160,549,809 (GRCm39)	V266L	probably damaging	Het
Reep6	A	G	10: 80,171,009 (GRCm39)	D295G	probably damaging	Het
Sass6	T	C	3: 116,403,947 (GRCm39)	L195P	probably damaging	Het
Slc25a27	A	G	17: 43,977,821 (GRCm39)	S25P	probably damaging	Het
Smok2b	T	A	17: 13,454,011 (GRCm39)	M57K	probably benign	Het
Sp110	C	G	1: 85,516,839 (GRCm39)	E219D	probably damaging	Het
Sspo	T	C	6: 48,465,405 (GRCm39)	V71A	possibly damaging	Het
Stab1	G	A	14: 30,881,261 (GRCm39)	L590F	probably damaging	Het
Stard9	T	A	2: 120,527,500 (GRCm39)	Y1252*	probably null	Het
Syde2	T	A	3: 145,712,881 (GRCm39)	D1006E	probably damaging	Het
Syde2	CAGTT	CAGTTAGTT	3: 145,707,163 (GRCm39)		probably null	Het
Tmc5	G	A	7: 118,244,609 (GRCm39)	A496T	possibly damaging	Het
Ttn	A	G	2: 76,566,704 (GRCm39)	V28063A	probably damaging	Het
Usp25	A	C	16: 76,874,007 (GRCm39)	I528L	probably benign	Het
Vmn1r58	C	T	7: 5,413,666 (GRCm39)	S188N	probably benign	Het
Wfikkn2	A	T	11: 94,129,173 (GRCm39)	C323S	probably damaging	Het
Zfy1	A	C	Y: 732,964 (GRCm39)	S290A	unknown	Het

Other mutations in Or2g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01862:Or2g25	APN	17	37,970,368 (GRCm39)	missense	probably damaging	1.00
IGL01949:Or2g25	APN	17	37,970,357 (GRCm39)	missense	probably damaging	1.00
IGL02085:Or2g25	APN	17	37,970,579 (GRCm39)	missense	probably benign	0.11
IGL02481:Or2g25	APN	17	37,970,363 (GRCm39)	missense	probably damaging	1.00
IGL02483:Or2g25	APN	17	37,970,363 (GRCm39)	missense	probably damaging	1.00
IGL03274:Or2g25	APN	17	37,970,646 (GRCm39)	missense	probably benign	0.35
R0234:Or2g25	UTSW	17	37,970,997 (GRCm39)	missense	probably damaging	0.99
R0234:Or2g25	UTSW	17	37,970,997 (GRCm39)	missense	probably damaging	0.99
R1522:Or2g25	UTSW	17	37,970,661 (GRCm39)	missense	probably damaging	1.00
R1712:Or2g25	UTSW	17	37,970,799 (GRCm39)	missense	probably benign	0.42
R1750:Or2g25	UTSW	17	37,970,564 (GRCm39)	missense	probably damaging	1.00
R1865:Or2g25	UTSW	17	37,970,754 (GRCm39)	missense	possibly damaging	0.78
R2371:Or2g25	UTSW	17	37,971,044 (GRCm39)	missense	probably damaging	1.00
R2382:Or2g25	UTSW	17	37,970,822 (GRCm39)	missense	probably benign	0.00
R3798:Or2g25	UTSW	17	37,970,997 (GRCm39)	missense	probably damaging	0.99
R4831:Or2g25	UTSW	17	37,970,969 (GRCm39)	missense	probably benign	0.03
R5365:Or2g25	UTSW	17	37,970,586 (GRCm39)	missense	probably damaging	1.00
R5812:Or2g25	UTSW	17	37,970,630 (GRCm39)	missense	probably damaging	1.00
R5822:Or2g25	UTSW	17	37,971,122 (GRCm39)	missense	probably damaging	1.00
R6405:Or2g25	UTSW	17	37,971,014 (GRCm39)	missense	possibly damaging	0.58
R6945:Or2g25	UTSW	17	37,970,405 (GRCm39)	missense	possibly damaging	0.95
R7121:Or2g25	UTSW	17	37,970,699 (GRCm39)	missense	probably damaging	0.98
R7312:Or2g25	UTSW	17	37,970,403 (GRCm39)	missense	possibly damaging	0.78
R7502:Or2g25	UTSW	17	37,971,122 (GRCm39)	missense	probably damaging	1.00
R8116:Or2g25	UTSW	17	37,970,631 (GRCm39)	missense	probably damaging	1.00
R8425:Or2g25	UTSW	17	37,970,975 (GRCm39)	missense	probably damaging	1.00
R8960:Or2g25	UTSW	17	37,970,760 (GRCm39)	missense	probably benign	0.02
R9168:Or2g25	UTSW	17	37,971,047 (GRCm39)	missense	probably damaging	1.00
RF017:Or2g25	UTSW	17	37,970,672 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACTTTCCCTGACTCTGTGCATAG -3'
(R):5'- TCTGCCAATCTCTTGCAGGAG -3'

Sequencing Primer
(F):5'- CCTGACTCTGTGCATAGGAGCTG -3'
(R):5'- CAATCTCTTGCAGGAGTGGCG -3'

Posted On

2016-06-06