Mutagenetix > Incidental Mutation

Incidental Mutation 'R5090:Lama3'

387723

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

042679-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5090 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12333819-12583013 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 12542402 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 1015 (T1015A)

Ref Sequence

ENSEMBL: ENSMUSP00000140104 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070] [ENSMUST00000188815]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000092070
AA Change: T2621A

PolyPhen 2 Score 0.076 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: T2621A

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000188815
AA Change: T1015A

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000140104
Gene: ENSMUSG00000024421
AA Change: T1015A

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_Lam	78	122	2.66e-10	SMART
EGF_Lam	125	175	7.81e-8	SMART
Pfam:Laminin_I	230	496	1e-90	PFAM
low complexity region	579	594	N/A	INTRINSIC
coiled coil region	605	632	N/A	INTRINSIC
LamG	800	960	1.67e-2	SMART
LamG	1008	1136	1.72e-17	SMART
LamG	1179	1294	3.96e-17	SMART
LamG	1399	1527	1.12e-34	SMART
LamG	1569	1702	3.41e-30	SMART

Meta Mutation Damage Score

0.0633

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.2%

Validation Efficiency

99% (66/67)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	A	G	7: 120,385,199 (GRCm38)	S1168G	probably damaging	Het
Acsf2	A	T	11: 94,571,269 (GRCm38)		probably null	Het
AI481877	T	C	4: 59,111,108 (GRCm38)	T55A	unknown	Het
Ankk1	G	T	9: 49,421,763 (GRCm38)	S140R	probably damaging	Het
Ash1l	G	T	3: 89,052,877 (GRCm38)	K2305N	probably damaging	Het
Asnsd1	C	T	1: 53,352,404 (GRCm38)		probably benign	Het
Atxn7l1	T	C	12: 33,326,078 (GRCm38)	S51P	probably damaging	Het
Ccdc88c	A	G	12: 100,954,180 (GRCm38)	L394P	probably damaging	Het
Cd200r1	T	A	16: 44,789,561 (GRCm38)	S48T	possibly damaging	Het
Cemip	T	C	7: 83,942,135 (GRCm38)	E1243G	probably damaging	Het
Cep192	T	A	18: 67,860,546 (GRCm38)	H1977Q	possibly damaging	Het
Cep250	T	C	2: 155,976,404 (GRCm38)	L833P	probably damaging	Het
Chd9	C	A	8: 91,026,834 (GRCm38)	Y1818*	probably null	Het
Clca3a1	C	A	3: 144,737,872 (GRCm38)	V706L	probably benign	Het
Cntln	T	C	4: 84,947,593 (GRCm38)	V162A	probably damaging	Het
Col5a1	G	A	2: 28,018,602 (GRCm38)	W67*	probably null	Het
Cux1	T	A	5: 136,313,200 (GRCm38)	N446I	possibly damaging	Het
Dlg1	G	T	16: 31,838,084 (GRCm38)	G599W	probably damaging	Het
Dock7	C	T	4: 98,991,411 (GRCm38)	V969I	probably benign	Het
Ephb3	T	C	16: 21,214,487 (GRCm38)	C74R	probably damaging	Het
Fads6	T	A	11: 115,296,654 (GRCm38)	T72S	probably benign	Het
Fra10ac1	C	T	19: 38,214,425 (GRCm38)	R110Q	probably damaging	Het
Gdf3	T	A	6: 122,609,754 (GRCm38)	L71F	probably benign	Het
Gm1966	T	A	7: 106,600,902 (GRCm38)		noncoding transcript	Het
Gm6685	A	G	11: 28,339,253 (GRCm38)	Y188H	probably benign	Het
Gm6981	A	C	9: 52,002,842 (GRCm38)		noncoding transcript	Het
Gnpda1	T	C	18: 38,332,093 (GRCm38)	T157A	probably damaging	Het
Grap2	A	C	15: 80,638,482 (GRCm38)	N70H	possibly damaging	Het
Hdac5	G	A	11: 102,197,713 (GRCm38)	R887C	probably damaging	Het
Hectd3	T	A	4: 117,000,238 (GRCm38)		probably benign	Het
Hmgcr	T	C	13: 96,650,590 (GRCm38)	K162E	probably benign	Het
Inpp5e	A	T	2: 26,399,371 (GRCm38)		probably null	Het
Kifap3	A	G	1: 163,856,076 (GRCm38)	D442G	possibly damaging	Het
Lcp2	T	A	11: 34,089,725 (GRCm38)	Y508*	probably null	Het
Map1b	G	T	13: 99,430,026 (GRCm38)	Y2062*	probably null	Het
Mipep	A	G	14: 60,802,299 (GRCm38)	D259G	possibly damaging	Het
Mmp2	T	C	8: 92,852,574 (GRCm38)	F97S	probably damaging	Het
Mrpl18	A	C	17: 12,913,810 (GRCm38)	M144R	probably damaging	Het
Nek9	A	G	12: 85,329,842 (GRCm38)		probably null	Het
Nmral1	A	T	16: 4,714,531 (GRCm38)	Y139N	probably damaging	Het
Notch4	T	C	17: 34,580,920 (GRCm38)	C952R	probably damaging	Het
Nsf	T	C	11: 103,910,578 (GRCm38)	N204D	probably benign	Het
P2rx4	A	T	5: 122,725,055 (GRCm38)	D197V	probably damaging	Het
Pak7	A	T	2: 136,087,418 (GRCm38)	I615N	probably damaging	Het
Parp10	T	C	15: 76,241,725 (GRCm38)	D421G	probably damaging	Het
Pcdha6	A	T	18: 36,968,717 (GRCm38)	D321V	probably benign	Het
Pkhd1	T	A	1: 20,200,757 (GRCm38)	I3191F	probably damaging	Het
Psmd4	T	C	3: 95,035,248 (GRCm38)	N7S	possibly damaging	Het
Ptprq	A	G	10: 107,526,089 (GRCm38)	V2084A	probably damaging	Het
Rab3gap1	A	G	1: 127,915,678 (GRCm38)	E263G	probably benign	Het
Rbm5	A	G	9: 107,760,312 (GRCm38)		probably benign	Het
Sacs	T	C	14: 61,205,253 (GRCm38)	F1583L	probably damaging	Het
Sel1l3	G	T	5: 53,200,046 (GRCm38)	H201Q	probably benign	Het
Tdpoz3	T	A	3: 93,826,563 (GRCm38)	W182R	possibly damaging	Het
Trim55	A	G	3: 19,671,607 (GRCm38)	N313S	probably benign	Het
Usp17lb	A	T	7: 104,841,083 (GRCm38)	D212E	probably benign	Het
Xirp2	A	G	2: 67,525,470 (GRCm38)	E3525G	possibly damaging	Het
Zfp236	T	C	18: 82,618,881 (GRCm38)	T1379A	probably benign	Het
Zfp553	A	T	7: 127,235,487 (GRCm38)	E71D	probably damaging	Het
Znrf1	T	G	8: 111,538,403 (GRCm38)	F21V	probably benign	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,580,292 (GRCm38)	missense	probably benign
IGL00272:Lama3	APN	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,449,588 (GRCm38)	splice site	probably benign
IGL00836:Lama3	APN	18	12,472,228 (GRCm38)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,441,143 (GRCm38)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,481,037 (GRCm38)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,531,926 (GRCm38)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,441,131 (GRCm38)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,453,880 (GRCm38)	splice site	probably benign
IGL01863:Lama3	APN	18	12,419,936 (GRCm38)	splice site	probably benign
IGL01869:Lama3	APN	18	12,524,763 (GRCm38)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,572,064 (GRCm38)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,516,513 (GRCm38)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,468,314 (GRCm38)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,581,783 (GRCm38)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,491,476 (GRCm38)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,556,750 (GRCm38)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,557,727 (GRCm38)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,537,858 (GRCm38)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,525,853 (GRCm38)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,578,127 (GRCm38)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,528,801 (GRCm38)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,468,368 (GRCm38)	splice site	probably benign
IGL03038:Lama3	APN	18	12,419,250 (GRCm38)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,439,349 (GRCm38)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,527,624 (GRCm38)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,481,038 (GRCm38)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,539,703 (GRCm38)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,553,283 (GRCm38)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,419,182 (GRCm38)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,553,231 (GRCm38)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,539,967 (GRCm38)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,497,881 (GRCm38)	splice site	probably benign
R0007:Lama3	UTSW	18	12,497,881 (GRCm38)	splice site	probably benign
R0050:Lama3	UTSW	18	12,404,103 (GRCm38)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,404,103 (GRCm38)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,528,705 (GRCm38)	splice site	probably benign
R0063:Lama3	UTSW	18	12,528,705 (GRCm38)	splice site	probably benign
R0106:Lama3	UTSW	18	12,403,982 (GRCm38)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,448,272 (GRCm38)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,524,810 (GRCm38)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,539,823 (GRCm38)	splice site	probably null
R0240:Lama3	UTSW	18	12,539,823 (GRCm38)	splice site	probably null
R0316:Lama3	UTSW	18	12,519,877 (GRCm38)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,482,126 (GRCm38)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,507,007 (GRCm38)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,407,563 (GRCm38)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,456,837 (GRCm38)	missense	probably benign
R0449:Lama3	UTSW	18	12,500,512 (GRCm38)	splice site	probably null
R0453:Lama3	UTSW	18	12,465,478 (GRCm38)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,450,424 (GRCm38)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,525,894 (GRCm38)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,561,701 (GRCm38)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,549,252 (GRCm38)	missense	probably benign
R0605:Lama3	UTSW	18	12,506,949 (GRCm38)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,419,258 (GRCm38)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,419,245 (GRCm38)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,477,590 (GRCm38)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,456,850 (GRCm38)	splice site	probably benign
R1216:Lama3	UTSW	18	12,421,134 (GRCm38)	splice site	probably benign
R1356:Lama3	UTSW	18	12,500,577 (GRCm38)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,477,370 (GRCm38)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,519,991 (GRCm38)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,481,098 (GRCm38)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,549,227 (GRCm38)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,441,107 (GRCm38)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,441,107 (GRCm38)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,482,045 (GRCm38)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,513,731 (GRCm38)	splice site	probably benign
R1571:Lama3	UTSW	18	12,539,717 (GRCm38)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,450,400 (GRCm38)	nonsense	probably null
R1631:Lama3	UTSW	18	12,407,494 (GRCm38)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,532,199 (GRCm38)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,532,199 (GRCm38)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,479,872 (GRCm38)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,465,499 (GRCm38)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,402,062 (GRCm38)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,513,705 (GRCm38)	missense	possibly damaging	0.75
R1856:Lama3	UTSW	18	12,537,781 (GRCm38)	nonsense	probably null
R1909:Lama3	UTSW	18	12,581,798 (GRCm38)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,495,279 (GRCm38)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,453,863 (GRCm38)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,524,721 (GRCm38)	splice site	probably benign
R2059:Lama3	UTSW	18	12,528,333 (GRCm38)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,528,726 (GRCm38)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,524,830 (GRCm38)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,402,849 (GRCm38)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,525,079 (GRCm38)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,413,858 (GRCm38)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,448,288 (GRCm38)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,439,475 (GRCm38)	splice site	probably benign
R3752:Lama3	UTSW	18	12,507,029 (GRCm38)	missense	probably damaging	1.00
R3967:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,504,308 (GRCm38)	nonsense	probably null
R4118:Lama3	UTSW	18	12,450,431 (GRCm38)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,513,690 (GRCm38)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,582,531 (GRCm38)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,519,872 (GRCm38)	nonsense	probably null
R4483:Lama3	UTSW	18	12,549,253 (GRCm38)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,481,088 (GRCm38)	missense	probably benign
R4516:Lama3	UTSW	18	12,495,358 (GRCm38)	missense	probably damaging	1.00
R4556:Lama3	UTSW	18	12,479,759 (GRCm38)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,504,397 (GRCm38)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,578,029 (GRCm38)	nonsense	probably null
R4704:Lama3	UTSW	18	12,553,223 (GRCm38)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,504,359 (GRCm38)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,482,084 (GRCm38)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,500,563 (GRCm38)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,413,771 (GRCm38)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,411,570 (GRCm38)	nonsense	probably null
R4784:Lama3	UTSW	18	12,449,544 (GRCm38)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,477,604 (GRCm38)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,441,131 (GRCm38)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,411,542 (GRCm38)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,498,678 (GRCm38)	intron	probably benign
R4863:Lama3	UTSW	18	12,539,793 (GRCm38)	missense	probably damaging	0.99
R4953:Lama3	UTSW	18	12,448,305 (GRCm38)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,552,826 (GRCm38)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,518,743 (GRCm38)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,582,611 (GRCm38)	missense	probably benign	0.19
R5057:Lama3	UTSW	18	12,531,948 (GRCm38)	missense	probably null	0.82
R5122:Lama3	UTSW	18	12,539,766 (GRCm38)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,577,900 (GRCm38)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,419,893 (GRCm38)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,465,508 (GRCm38)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,552,855 (GRCm38)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,453,746 (GRCm38)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,572,066 (GRCm38)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,456,764 (GRCm38)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,553,210 (GRCm38)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,472,220 (GRCm38)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,439,348 (GRCm38)	missense	probably benign
R5617:Lama3	UTSW	18	12,498,936 (GRCm38)	intron	probably benign
R5709:Lama3	UTSW	18	12,539,799 (GRCm38)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,429,887 (GRCm38)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,574,254 (GRCm38)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,469,928 (GRCm38)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,482,099 (GRCm38)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,513,645 (GRCm38)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,524,737 (GRCm38)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,506,949 (GRCm38)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,482,137 (GRCm38)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,479,756 (GRCm38)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,482,148 (GRCm38)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,495,348 (GRCm38)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,537,766 (GRCm38)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,577,840 (GRCm38)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,419,257 (GRCm38)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,513,678 (GRCm38)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,549,226 (GRCm38)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,528,418 (GRCm38)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,516,548 (GRCm38)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,582,545 (GRCm38)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,582,644 (GRCm38)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,552,813 (GRCm38)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,531,879 (GRCm38)	missense	probably benign	0.00
R7121:Lama3	UTSW	18	12,462,782 (GRCm38)	missense	probably benign	0.06
R7133:Lama3	UTSW	18	12,539,786 (GRCm38)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,468,289 (GRCm38)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,456,812 (GRCm38)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,404,076 (GRCm38)	missense	probably benign	0.26
R7216:Lama3	UTSW	18	12,430,000 (GRCm38)	missense	probably damaging	1.00
R7223:Lama3	UTSW	18	12,582,608 (GRCm38)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,419,845 (GRCm38)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,439,392 (GRCm38)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,507,040 (GRCm38)	splice site	probably null
R7442:Lama3	UTSW	18	12,472,181 (GRCm38)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,419,237 (GRCm38)	missense	probably benign
R7604:Lama3	UTSW	18	12,500,493 (GRCm38)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,531,834 (GRCm38)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,537,838 (GRCm38)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,462,807 (GRCm38)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,537,739 (GRCm38)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,534,063 (GRCm38)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,506,942 (GRCm38)	missense	probably null
R8219:Lama3	UTSW	18	12,439,360 (GRCm38)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,407,551 (GRCm38)	missense	probably benign
R8229:Lama3	UTSW	18	12,407,551 (GRCm38)	missense	probably benign
R8298:Lama3	UTSW	18	12,525,853 (GRCm38)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,540,613 (GRCm38)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,528,347 (GRCm38)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,449,839 (GRCm38)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,411,631 (GRCm38)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,421,155 (GRCm38)	missense	probably benign
R8799:Lama3	UTSW	18	12,490,943 (GRCm38)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,449,586 (GRCm38)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,556,705 (GRCm38)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,532,039 (GRCm38)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,481,063 (GRCm38)	nonsense	probably null
R9126:Lama3	UTSW	18	12,450,470 (GRCm38)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,472,240 (GRCm38)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,462,812 (GRCm38)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,577,902 (GRCm38)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,450,484 (GRCm38)	nonsense	probably null
R9553:Lama3	UTSW	18	12,429,962 (GRCm38)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,549,263 (GRCm38)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,582,574 (GRCm38)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,429,879 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- TCTCTAAAGCGGGGTTTAAGAAG -3'
(R):5'- ATATGACTGGCAGCGTCACC -3'

Sequencing Primer
(F):5'- GGCTAAACATGTTAACAGTTTCCCC -3'
(R):5'- ACCCTGCGGCAGCTTTC -3'

Posted On

2016-06-06