|Institutional Source||Beutler Lab|
|Gene Name||free fatty acid receptor 4|
|Synonyms||Gpr129, O3far1, Pgr4, Gpr120|
|Is this an essential gene?||Probably non essential (E-score: 0.082)|
|Stock #||R5091 (G1)|
|Chromosomal Location||38097079-38114263 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 38097179 bp|
|Amino Acid Change||Aspartic acid to Glycine at position 18 (D18G)|
|Ref Sequence||ENSEMBL: ENSMUSP00000063660 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000067098]|
|Predicted Effect||probably benign
AA Change: D18G
PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
AA Change: D18G
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||96% (67/70)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
PHENOTYPE: Homozygotes for a null allele show altered taste responses to fatty acids. Homozygotes for another null allele develop obesity, liver steatosis, and impaired glucose metabolism, adipogenesis and lipogenesis on a high-fat diet. Homozygotes for a third allele show altered islet somatostatin secretion. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ffar4||
(F):5'- ACTTAGACTGCCCAAGGGTCTG -3'
(R):5'- AATCCGCGCAGAAGAGGTTG -3'
(F):5'- AGTGTTGCCAGGCACTTC -3'
(R):5'- CGCAGAAGAGGTTGAGCAC -3'