Incidental Mutation 'R5099:Slfn3'
ID 388183
Institutional Source Beutler Lab
Gene Symbol Slfn3
Ensembl Gene ENSMUSG00000018986
Gene Name schlafen 3
Synonyms
MMRRC Submission 042688-MU
Accession Numbers

MGI: 1329005
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5099 (G1)
Quality Score 225
Status Validated
Chromosome 11
Chromosomal Location 83191330-83215154 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to T at 83214938 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Phenylalanine at position 587 (Y587F)
Ref Sequence ENSEMBL: ENSMUSP00000150425 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000019130] [ENSMUST00000214041]
AlphaFold A0A1L1STQ7
Predicted Effect probably damaging
Transcript: ENSMUST00000019130
AA Change: Y464F

PolyPhen 2 Score 0.961 (Sensitivity: 0.78; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000019130
Gene: ENSMUSG00000018986
AA Change: Y464F

DomainStartEndE-ValueType
Pfam:AlbA_2 165 303 5.5e-11 PFAM
low complexity region 394 412 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000214041
AA Change: Y587F

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000216599
Meta Mutation Damage Score 0.5653 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.2%
Validation Efficiency 98% (41/42)
MGI Phenotype PHENOTYPE: Mice homozygous for a targeted allele exhibit normal immune cell populations. [provided by MGI curators]
Allele List at MGI

All alleles(3) : Targeted(3)
Other mutations in this stock
Total: 37 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700017D01Rik T C 19: 11,112,461 probably null Het
Arsa A G 15: 89,475,339 L80P probably damaging Het
Bdkrb1 A G 12: 105,604,274 D33G probably benign Het
Ccdc178 A T 18: 22,105,591 V323E probably benign Het
Ceacam5 T C 7: 17,745,588 V210A probably damaging Het
Dcdc2a A G 13: 25,107,698 E222G probably benign Het
Gbp9 A T 5: 105,094,513 L120Q probably damaging Het
Gm4787 G C 12: 81,377,830 T518S probably benign Het
Gsr T A 8: 33,671,528 I121N probably damaging Het
Ift140 T A 17: 25,090,700 M1068K probably damaging Het
Jakmip2 A G 18: 43,568,108 I414T probably benign Het
Lpin2 G A 17: 71,243,970 W708* probably null Het
Mecom A G 3: 29,985,316 probably benign Het
Mff C T 1: 82,750,471 probably benign Het
Neb C T 2: 52,195,448 C1545Y probably damaging Het
Olfr1083-ps A T 2: 86,607,216 N118K unknown Het
Olfr206 C T 16: 59,344,903 G266D probably benign Het
Olfr467 A G 7: 107,814,602 H6R probably benign Het
Ppm1n A T 7: 19,277,978 L392Q possibly damaging Het
Prr22 T C 17: 56,771,467 F207L probably benign Het
Ptprv T C 1: 135,118,854 noncoding transcript Het
Rin2 G A 2: 145,878,901 C718Y probably damaging Het
Rpgrip1l G A 8: 91,248,722 T1089I probably benign Het
Scn1a A G 2: 66,277,801 V1510A probably damaging Het
Sp2 T C 11: 96,961,349 K250E probably damaging Het
Ssu2 A G 6: 112,359,624 S333P probably benign Het
Strbp T C 2: 37,603,018 T419A probably damaging Het
Tada2b A T 5: 36,476,400 M203K probably benign Het
Tcrg-V5 G T 13: 19,192,716 C111F probably damaging Het
Tmem176b T C 6: 48,834,529 Y62C probably benign Het
Tox G T 4: 6,688,958 Q469K probably benign Het
Tyw5 T C 1: 57,388,705 N243D probably damaging Het
Ube2q2 T A 9: 55,206,023 probably benign Het
Ufl1 C T 4: 25,275,914 R83Q probably damaging Het
Unk A G 11: 116,059,110 Q701R probably benign Het
Vmn1r60 A T 7: 5,544,817 C95S probably damaging Het
Vmn1r81 A G 7: 12,260,321 I120T possibly damaging Het
Other mutations in Slfn3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00843:Slfn3 APN 11 83213431 missense probably damaging 1.00
IGL01405:Slfn3 APN 11 83214716 missense possibly damaging 0.90
IGL01631:Slfn3 APN 11 83213535 missense probably damaging 0.99
IGL01944:Slfn3 APN 11 83213148 missense possibly damaging 0.59
IGL02354:Slfn3 APN 11 83213242 missense possibly damaging 0.95
IGL02361:Slfn3 APN 11 83213242 missense possibly damaging 0.95
IGL02512:Slfn3 APN 11 83213025 missense possibly damaging 0.55
IGL02875:Slfn3 APN 11 83213427 missense probably damaging 0.98
IGL02944:Slfn3 APN 11 83213011 missense probably damaging 0.99
IGL03402:Slfn3 APN 11 83213431 missense probably damaging 1.00
R0452:Slfn3 UTSW 11 83213128 missense possibly damaging 0.87
R0506:Slfn3 UTSW 11 83213160 missense probably damaging 0.99
R0560:Slfn3 UTSW 11 83213152 missense probably damaging 0.99
R0788:Slfn3 UTSW 11 83212836 missense possibly damaging 0.47
R1602:Slfn3 UTSW 11 83212715 missense probably damaging 1.00
R1713:Slfn3 UTSW 11 83213314 missense probably damaging 0.98
R1881:Slfn3 UTSW 11 83213376 missense possibly damaging 0.80
R2264:Slfn3 UTSW 11 83212972 missense probably benign 0.00
R2441:Slfn3 UTSW 11 83212683 missense probably benign 0.00
R2921:Slfn3 UTSW 11 83215045 missense probably benign 0.01
R4163:Slfn3 UTSW 11 83212770 missense probably damaging 1.00
R5448:Slfn3 UTSW 11 83214605 missense probably damaging 0.99
R6441:Slfn3 UTSW 11 83214914 missense probably benign 0.00
R6527:Slfn3 UTSW 11 83213106 missense probably benign 0.01
R6785:Slfn3 UTSW 11 83214601 missense possibly damaging 0.73
R7128:Slfn3 UTSW 11 83214895 missense probably benign 0.00
R7344:Slfn3 UTSW 11 83212822 missense probably benign 0.28
R7528:Slfn3 UTSW 11 83214905 missense probably benign 0.01
R7763:Slfn3 UTSW 11 83214788 missense possibly damaging 0.95
R8155:Slfn3 UTSW 11 83212785 missense probably damaging 1.00
R8178:Slfn3 UTSW 11 83214679 missense probably benign 0.33
R8210:Slfn3 UTSW 11 83214506 missense possibly damaging 0.48
R8347:Slfn3 UTSW 11 83213589 missense possibly damaging 0.95
R8671:Slfn3 UTSW 11 83212999 missense probably benign 0.00
R9093:Slfn3 UTSW 11 83213122 missense probably damaging 0.99
R9106:Slfn3 UTSW 11 83212632 missense probably benign 0.00
R9293:Slfn3 UTSW 11 83214790 missense possibly damaging 0.85
R9362:Slfn3 UTSW 11 83212981 missense probably benign
R9521:Slfn3 UTSW 11 83212999 missense probably benign
R9522:Slfn3 UTSW 11 83212999 missense probably benign
R9644:Slfn3 UTSW 11 83214902 missense probably damaging 1.00
Z1176:Slfn3 UTSW 11 83213409 missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- AACTAGGCGCCGAGTTAAAG -3'
(R):5'- ACTGTGAAACATCATTGTGCC -3'

Sequencing Primer
(F):5'- CGCCGAGTTAAAGGGCTATTATAAAC -3'
(R):5'- GTGAAACATCATTGTGCCATACTCC -3'
Posted On 2016-06-06