Incidental Mutation 'R5069:Thoc2'
ID388597
Institutional Source Beutler Lab
Gene Symbol Thoc2
Ensembl Gene ENSMUSG00000037475
Gene NameTHO complex 2
SynonymsLOC382210, 6330441O12Rik, LOC386493, D130005M13Rik
MMRRC Submission 042659-MU
Accession Numbers
Is this an essential gene? Not available question?
Stock #R5069 (G1)
Quality Score222
Status Not validated
ChromosomeX
Chromosomal Location41794991-41920674 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to T at 41806693 bp
ZygosityHeterozygous
Amino Acid Change Glutamic Acid to Lysine at position 1491 (E1491K)
Ref Sequence ENSEMBL: ENSMUSP00000044677 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000047037] [ENSMUST00000124458] [ENSMUST00000155369]
Predicted Effect probably damaging
Transcript: ENSMUST00000047037
AA Change: E1491K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000044677
Gene: ENSMUSG00000037475
AA Change: E1491K

DomainStartEndE-ValueType
Pfam:THOC2_N 8 422 5.6e-66 PFAM
Pfam:THOC2_N 415 566 2.1e-31 PFAM
Pfam:Thoc2 568 643 1.3e-39 PFAM
low complexity region 729 747 N/A INTRINSIC
Pfam:Tho2 873 1173 3.3e-109 PFAM
low complexity region 1203 1219 N/A INTRINSIC
low complexity region 1251 1284 N/A INTRINSIC
low complexity region 1300 1313 N/A INTRINSIC
low complexity region 1323 1343 N/A INTRINSIC
low complexity region 1356 1367 N/A INTRINSIC
coiled coil region 1464 1491 N/A INTRINSIC
low complexity region 1525 1553 N/A INTRINSIC
low complexity region 1566 1574 N/A INTRINSIC
low complexity region 1577 1590 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000124458
AA Change: E129K

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000119460
Gene: ENSMUSG00000037475
AA Change: E129K

DomainStartEndE-ValueType
coiled coil region 102 129 N/A INTRINSIC
low complexity region 163 191 N/A INTRINSIC
low complexity region 204 212 N/A INTRINSIC
low complexity region 215 228 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000134084
Predicted Effect unknown
Transcript: ENSMUST00000143557
AA Change: E13K
SMART Domains Protein: ENSMUSP00000118106
Gene: ENSMUSG00000037475
AA Change: E13K

DomainStartEndE-ValueType
low complexity region 48 76 N/A INTRINSIC
low complexity region 89 97 N/A INTRINSIC
low complexity region 100 113 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000145586
SMART Domains Protein: ENSMUSP00000118960
Gene: ENSMUSG00000037475

DomainStartEndE-ValueType
low complexity region 19 47 N/A INTRINSIC
low complexity region 60 68 N/A INTRINSIC
low complexity region 70 84 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000151430
SMART Domains Protein: ENSMUSP00000115815
Gene: ENSMUSG00000037475

DomainStartEndE-ValueType
low complexity region 3 20 N/A INTRINSIC
low complexity region 36 49 N/A INTRINSIC
low complexity region 59 79 N/A INTRINSIC
low complexity region 92 103 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000155369
AA Change: E8K

PolyPhen 2 Score 0.659 (Sensitivity: 0.86; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000120342
Gene: ENSMUSG00000037475
AA Change: E8K

DomainStartEndE-ValueType
low complexity region 42 70 N/A INTRINSIC
low complexity region 83 91 N/A INTRINSIC
low complexity region 93 107 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 93.5%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The TREX multiprotein complex binds specifically to spliced mRNAs to facilitate mRNA export. The protein encoded by this gene is a member of the THO complex, a subset of the TREX complex. The encoded protein interacts with the THOC1 protein.[provided by RefSeq, Jun 2010]
Allele List at MGI
Other mutations in this stock
Total: 77 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3425401B19Rik A G 14: 32,661,792 S739P possibly damaging Het
9330182L06Rik T A 5: 9,440,897 C636S probably damaging Het
Actn2 A G 13: 12,288,522 I464T possibly damaging Het
Adcy7 T C 8: 88,327,697 L1060P probably damaging Het
Aff3 A G 1: 38,181,613 probably null Het
Ankar T C 1: 72,680,210 probably null Het
Ankrd27 A T 7: 35,628,435 K793N probably damaging Het
Arhgef28 T C 13: 98,075,206 T90A probably damaging Het
Armc9 A T 1: 86,257,237 H670L probably benign Het
Arvcf A G 16: 18,398,986 Y412C probably damaging Het
Ass1 C T 2: 31,510,173 T301M probably damaging Het
Baiap3 A T 17: 25,249,108 C283S probably damaging Het
BC055324 T C 1: 163,987,674 T93A possibly damaging Het
Birc6 C T 17: 74,565,972 R409C probably damaging Het
Calcoco1 A G 15: 102,711,092 L354P probably damaging Het
Cdh3 A G 8: 106,536,826 N126S probably benign Het
Cfap54 A C 10: 92,937,774 F135L probably benign Het
Dlg1 G T 16: 31,684,295 probably null Het
Dnah3 T C 7: 120,032,790 H1314R probably benign Het
Dsp A C 13: 38,197,123 T2615P possibly damaging Het
Enpp2 T C 15: 54,864,054 Y513C probably damaging Het
Ercc6 T C 14: 32,570,063 V1128A probably benign Het
Gipc2 A G 3: 152,094,248 F282L probably benign Het
Gm1043 T G 5: 37,187,236 L231R probably damaging Het
Gm13178 T A 4: 144,703,867 D184V probably damaging Het
Gm14085 A T 2: 122,494,373 N142I possibly damaging Het
Gm14685 T C X: 73,127,971 I323T probably damaging Het
Gpr153 T A 4: 152,279,883 M132K probably damaging Het
Hbs1l T A 10: 21,354,647 S496T probably damaging Het
Inpp5f A G 7: 128,676,727 probably null Het
Kat6a G A 8: 22,903,133 C209Y probably damaging Het
Kcna2 T A 3: 107,104,637 V178D probably damaging Het
Krt75 A G 15: 101,566,238 probably null Het
Letm2 G A 8: 25,593,964 Q84* probably null Het
Mib1 A G 18: 10,793,002 E646G probably damaging Het
Mmp14 T A 14: 54,439,113 Y372N probably damaging Het
Muc6 A G 7: 141,651,299 C218R probably damaging Het
Myof A T 19: 37,905,325 I1130N possibly damaging Het
Neil3 A G 8: 53,601,041 S318P possibly damaging Het
Nhlh1 A G 1: 172,053,900 V133A probably benign Het
Nup153 A C 13: 46,709,792 S331A probably benign Het
Nup98 T C 7: 102,145,655 T882A probably benign Het
Olfr1413 T A 1: 92,573,413 S81T probably damaging Het
Olfr599 A T 7: 103,338,022 probably null Het
Olfr744 T A 14: 50,618,740 C173S probably damaging Het
Olfr749 C A 14: 50,737,074 L29F probably benign Het
Otx1 C A 11: 21,997,037 A91S probably damaging Het
Pald1 A T 10: 61,341,246 M675K possibly damaging Het
Pex5 A G 6: 124,413,596 S97P probably benign Het
Pitpnm1 C T 19: 4,111,140 A897V probably benign Het
Plxnd1 A T 6: 115,965,901 V1274E probably damaging Het
Polr2a T C 11: 69,736,735 probably null Het
Ppfia1 G A 7: 144,514,473 Q446* probably null Het
Psma2 G A 13: 14,616,028 V20I probably benign Het
Rhbdl2 T A 4: 123,817,917 L149* probably null Het
Rnf17 T C 14: 56,505,928 V1317A probably damaging Het
Rtel1 G A 2: 181,355,492 V1042M probably benign Het
Sidt2 A T 9: 45,939,461 probably null Het
Slc11a1 G A 1: 74,385,184 A434T probably damaging Het
Slc4a10 G A 2: 62,267,571 R508H probably benign Het
Slc5a8 A T 10: 88,886,598 I98F possibly damaging Het
Slf2 T A 19: 44,935,253 S169T possibly damaging Het
Snx33 A T 9: 56,926,191 I198N probably damaging Het
Spock3 A G 8: 63,355,265 T396A probably benign Het
Sva A T 6: 42,038,417 probably benign Het
Syt7 A G 19: 10,439,237 N261S probably benign Het
Taar7b T G 10: 24,000,461 S175A probably benign Het
Tlr1 T C 5: 64,926,400 Y278C probably benign Het
Tph2 T A 10: 115,151,174 Y237F probably benign Het
Trim35 C T 14: 66,308,972 probably benign Het
Trpm4 T G 7: 45,310,469 Y667S probably damaging Het
Ttc27 T A 17: 74,799,342 H541Q probably damaging Het
Ube4a T C 9: 44,940,089 H709R probably damaging Het
Vwa7 G A 17: 35,024,190 V615I probably benign Het
Wars2 A G 3: 99,187,533 H48R probably damaging Het
Xlr4c T A X: 73,238,684 K121M probably damaging Het
Zfc3h1 T A 10: 115,418,783 C1427* probably null Het
Other mutations in Thoc2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00481:Thoc2 APN X 41879891 missense possibly damaging 0.86
IGL01313:Thoc2 APN X 41827346 missense probably benign 0.05
IGL02377:Thoc2 APN X 41825478 missense possibly damaging 0.73
R0079:Thoc2 UTSW X 41864108 missense probably benign 0.19
Predicted Primers PCR Primer
(F):5'- AGACTCACATGGACTCTCACTTTTG -3'
(R):5'- CAGCAGTCTGTTTCAAGTTTTAGCTG -3'

Sequencing Primer
(F):5'- TTTGTGTTTTGAAACCCCCAC -3'
(R):5'- AGAACTTCAGTGTAGTAATCATGCC -3'
Posted On2016-06-06