Mutagenetix > Incidental Mutation

Incidental Mutation 'R5071:Fryl'

388758

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

2510002A14Rik, 2310004H21Rik, 9030227G01Rik

MMRRC Submission

042661-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.758) question?

Stock #

R5071 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

73019987-73256619 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 73074767 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 1550 (P1550L)

Ref Sequence

ENSEMBL: ENSMUSP00000098687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000094700
AA Change: P1550L

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: P1550L

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101127
AA Change: P1550L

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: P1550L

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156661

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000175890

Predicted Effect

probably benign
Transcript: ENSMUST00000201277

Predicted Effect

unknown
Transcript: ENSMUST00000202381
AA Change: P419L

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000202413

Predicted Effect

probably benign
Transcript: ENSMUST00000202697

Meta Mutation Damage Score

0.1496

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.0%
20x: 94.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 95 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy8	A	T	15: 64,787,358 (GRCm38)	W528R	probably damaging	Het
Agbl1	A	G	7: 76,421,917 (GRCm38)	E329G	probably damaging	Het
Agbl5	G	A	5: 30,903,059 (GRCm38)	R141Q	probably damaging	Het
AI182371	A	T	2: 35,085,215 (GRCm38)	I334N	probably benign	Het
Aldh1b1	A	G	4: 45,803,383 (GRCm38)		probably null	Het
Armc9	G	A	1: 86,186,116 (GRCm38)	M298I	probably benign	Het
Atp8a1	T	A	5: 67,815,723 (GRCm38)	K27N	probably benign	Het
BC004004	G	A	17: 29,294,415 (GRCm38)		probably null	Het
Ccdc83	A	T	7: 90,250,529 (GRCm38)	F45Y	probably damaging	Het
Cct8l1	G	A	5: 25,516,883 (GRCm38)	V199I	probably benign	Het
Cdc23	C	A	18: 34,651,689 (GRCm38)	V7L	unknown	Het
Cdh17	G	T	4: 11,810,325 (GRCm38)	G672V	probably damaging	Het
Cdh5	C	A	8: 104,140,702 (GRCm38)	T537K	probably damaging	Het
Chd1	T	A	17: 15,762,405 (GRCm38)	S1377R	probably benign	Het
Chd2	T	C	7: 73,429,689 (GRCm38)	N1821S	probably benign	Het
Cnot3	A	G	7: 3,650,861 (GRCm38)	K4R	probably damaging	Het
Colq	T	A	14: 31,528,832 (GRCm38)	I339F	possibly damaging	Het
Coq2	A	T	5: 100,667,950 (GRCm38)	L121Q	probably damaging	Het
Csnk1e	G	A	15: 79,420,872 (GRCm38)	R342*	probably null	Het
Cstl1	A	T	2: 148,750,927 (GRCm38)	M1L	probably benign	Het
Cwh43	A	G	5: 73,423,913 (GRCm38)		probably null	Het
Cyp2a22	A	T	7: 26,932,481 (GRCm38)	F450Y	probably benign	Het
Cyp2d10	C	T	15: 82,403,753 (GRCm38)	R383H	probably benign	Het
Cyp4a29	A	G	4: 115,247,663 (GRCm38)	T123A	probably benign	Het
Dgkh	T	C	14: 78,604,532 (GRCm38)	K417E	probably damaging	Het
Dnah11	A	T	12: 118,082,453 (GRCm38)	L1750*	probably null	Het
Edn1	A	G	13: 42,303,677 (GRCm38)	E62G	probably damaging	Het
Eef1akmt1	A	G	14: 57,566,007 (GRCm38)	L30P	probably damaging	Het
Fbxo42	T	C	4: 141,198,945 (GRCm38)	W313R	probably damaging	Het
Fign	A	T	2: 63,979,693 (GRCm38)	L411*	probably null	Het
Fitm1	T	A	14: 55,575,773 (GRCm38)	V31E	possibly damaging	Het
Gabrd	A	C	4: 155,387,162 (GRCm38)	F228C	probably damaging	Het
Gm5916	A	C	9: 36,128,668 (GRCm38)	L8R	probably benign	Het
Gm9573	G	A	17: 35,620,552 (GRCm38)		probably benign	Het
Gpr108	A	G	17: 57,235,335 (GRCm38)	S557P	probably damaging	Het
Gpr75	C	A	11: 30,892,380 (GRCm38)	N428K	probably damaging	Het
H2-M5	T	G	17: 36,987,184 (GRCm38)		probably null	Het
Hdac6	A	G	X: 7,944,797 (GRCm38)	F104L	probably damaging	Homo
Homer3	T	C	8: 70,291,355 (GRCm38)	V243A	probably benign	Het
Hspg2	T	C	4: 137,540,230 (GRCm38)	S2050P	probably damaging	Het
Hydin	A	G	8: 110,538,473 (GRCm38)	N2763D	probably benign	Het
Ifi203	T	C	1: 173,935,110 (GRCm38)	Q152R	possibly damaging	Het
Ifi44	T	C	3: 151,749,632 (GRCm38)		probably benign	Het
Ifi47	A	G	11: 49,095,534 (GRCm38)	T43A	probably benign	Het
Ighv1-24	A	G	12: 114,773,128 (GRCm38)	Y51H	probably benign	Het
Inf2	A	T	12: 112,612,039 (GRCm38)		probably null	Het
Insl6	T	C	19: 29,325,255 (GRCm38)	R21G	probably benign	Het
Kif19a	G	A	11: 114,767,227 (GRCm38)	M37I	probably benign	Het
Ltbp3	T	C	19: 5,756,823 (GRCm38)	C1043R	probably damaging	Het
Mkl1	A	G	15: 81,022,426 (GRCm38)	V91A	probably damaging	Het
Mpeg1	T	A	19: 12,461,181 (GRCm38)	M1K	probably null	Het
Ncald	A	T	15: 37,397,234 (GRCm38)	H67Q	probably damaging	Het
Nek9	C	A	12: 85,327,459 (GRCm38)	R268L	possibly damaging	Het
Nudt5	T	A	2: 5,864,387 (GRCm38)	H141Q	probably benign	Het
Numbl	G	A	7: 27,280,990 (GRCm38)	D466N	probably damaging	Het
Olfr1100	A	T	2: 86,978,322 (GRCm38)	V158D	possibly damaging	Het
Olfr1186	T	C	2: 88,526,041 (GRCm38)	S153P	probably damaging	Het
Olfr167	A	G	16: 19,515,027 (GRCm38)	I203T	probably benign	Het
Olfr310	A	G	7: 86,269,591 (GRCm38)	I66T	probably damaging	Het
Olfr606	A	T	7: 103,451,410 (GRCm38)	E24D	probably benign	Het
Olfr668	A	C	7: 104,925,493 (GRCm38)	N90K	probably benign	Het
Olfr986	T	C	9: 40,187,664 (GRCm38)	L183S	probably damaging	Het
Pik3c2g	T	A	6: 139,720,147 (GRCm38)	C65S	probably null	Het
Plagl1	A	G	10: 13,127,261 (GRCm38)	Y91C	probably damaging	Het
Polr1a	A	G	6: 71,931,709 (GRCm38)	N506S	possibly damaging	Het
Pomt2	A	C	12: 87,133,460 (GRCm38)	C256G	probably damaging	Het
Psg29	T	A	7: 17,211,838 (GRCm38)	D444E	probably damaging	Het
Ptgs1	A	T	2: 36,251,260 (GRCm38)	N573I	probably damaging	Het
Ptprf	C	A	4: 118,211,999 (GRCm38)	G1624W	probably damaging	Het
Rbm38	C	T	2: 173,022,082 (GRCm38)	P15S	probably benign	Het
Rc3h1	A	G	1: 160,959,477 (GRCm38)	D814G	possibly damaging	Het
Rnf40	T	C	7: 127,597,286 (GRCm38)	L802P	probably damaging	Het
Rusc2	A	G	4: 43,415,240 (GRCm38)	D182G	probably benign	Het
Setd1b	A	G	5: 123,160,914 (GRCm38)		probably benign	Het
Slc4a2	G	A	5: 24,438,762 (GRCm38)	S855N	probably benign	Het
Slc8a2	T	C	7: 16,150,583 (GRCm38)	L626P	possibly damaging	Het
Slirp	A	G	12: 87,444,014 (GRCm38)	T29A	probably damaging	Het
Smarcc2	A	G	10: 128,463,940 (GRCm38)	D158G	probably damaging	Het
Sptbn1	A	G	11: 30,113,854 (GRCm38)		probably null	Het
St3gal5	T	C	6: 72,132,053 (GRCm38)	C42R	probably damaging	Het
Syt7	T	C	19: 10,443,428 (GRCm38)	S397P	possibly damaging	Het
Tenm2	T	A	11: 36,068,381 (GRCm38)	T1114S	probably damaging	Het
Thada	T	A	17: 84,386,532 (GRCm38)	D1263V	probably damaging	Het
Tmco3	G	T	8: 13,292,860 (GRCm38)	E199*	probably null	Het
Tpcn1	A	T	5: 120,548,269 (GRCm38)		probably null	Het
Tppp2	G	A	14: 51,920,455 (GRCm38)	R119Q	probably benign	Het
Trim30d	A	C	7: 104,487,958 (GRCm38)	V13G	probably damaging	Het
Ubxn2b	A	T	4: 6,214,746 (GRCm38)	Q260L	probably damaging	Het
Ufl1	A	G	4: 25,254,780 (GRCm38)	Y559H	probably benign	Het
Usp5	A	G	6: 124,826,379 (GRCm38)	F7L	probably benign	Het
Utrn	A	T	10: 12,384,204 (GRCm38)		probably null	Het
Vmn1r28	A	T	6: 58,265,909 (GRCm38)	I246F	probably benign	Het
Vmn2r2	C	A	3: 64,116,900 (GRCm38)	M753I	probably benign	Het
Zbtb26	T	C	2: 37,435,929 (GRCm38)	H365R	probably benign	Het
Zfp976	A	T	7: 42,612,930 (GRCm38)	Y494*	probably null	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,148,108 (GRCm38)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,086,962 (GRCm38)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,054,597 (GRCm38)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,022,501 (GRCm38)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,122,364 (GRCm38)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,032,791 (GRCm38)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,124,769 (GRCm38)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,075,959 (GRCm38)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,110,176 (GRCm38)	splice site	probably benign
IGL02431:Fryl	APN	5	73,098,308 (GRCm38)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,065,293 (GRCm38)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,098,393 (GRCm38)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,069,877 (GRCm38)	intron	probably benign
IGL02642:Fryl	APN	5	73,095,466 (GRCm38)	missense	probably benign
IGL02657:Fryl	APN	5	73,054,860 (GRCm38)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,093,163 (GRCm38)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,059,383 (GRCm38)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,101,455 (GRCm38)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03183:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03275:Fryl	APN	5	73,148,033 (GRCm38)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,136,316 (GRCm38)	splice site	probably benign
IGL03341:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03343:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03350:Fryl	APN	5	73,133,306 (GRCm38)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,054,059 (GRCm38)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,110,281 (GRCm38)	splice site	probably benign
IGL03375:Fryl	APN	5	73,088,449 (GRCm38)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
Besotted	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,041,604 (GRCm38)	splice site	probably benign
R0312:Fryl	UTSW	5	73,072,888 (GRCm38)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,098,414 (GRCm38)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,086,972 (GRCm38)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,097,417 (GRCm38)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,064,497 (GRCm38)	splice site	probably benign
R0567:Fryl	UTSW	5	73,065,391 (GRCm38)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,124,734 (GRCm38)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,068,731 (GRCm38)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,065,359 (GRCm38)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,071,126 (GRCm38)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,089,196 (GRCm38)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,041,332 (GRCm38)	splice site	probably benign
R1076:Fryl	UTSW	5	73,124,673 (GRCm38)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,110,271 (GRCm38)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,064,925 (GRCm38)	splice site	probably benign
R1394:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,074,751 (GRCm38)	nonsense	probably null
R1664:Fryl	UTSW	5	73,059,435 (GRCm38)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,032,861 (GRCm38)	splice site	probably benign
R1937:Fryl	UTSW	5	73,133,367 (GRCm38)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,022,122 (GRCm38)	nonsense	probably null
R2036:Fryl	UTSW	5	73,107,962 (GRCm38)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,022,544 (GRCm38)	missense	probably benign
R2088:Fryl	UTSW	5	73,065,461 (GRCm38)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,122,299 (GRCm38)	missense	probably benign
R2106:Fryl	UTSW	5	73,098,331 (GRCm38)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,064,975 (GRCm38)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,108,547 (GRCm38)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,050,456 (GRCm38)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,082,850 (GRCm38)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,108,074 (GRCm38)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3787:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3837:Fryl	UTSW	5	73,071,265 (GRCm38)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,112,423 (GRCm38)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,086,560 (GRCm38)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,088,397 (GRCm38)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,081,053 (GRCm38)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,090,679 (GRCm38)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,100,293 (GRCm38)	missense	probably benign
R4700:Fryl	UTSW	5	73,065,538 (GRCm38)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,080,972 (GRCm38)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,041,362 (GRCm38)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,068,782 (GRCm38)	frame shift	probably null
R4948:Fryl	UTSW	5	73,089,130 (GRCm38)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,035,058 (GRCm38)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,075,893 (GRCm38)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,057,755 (GRCm38)	missense	probably benign	0.13
R5072:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,090,718 (GRCm38)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,101,673 (GRCm38)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,086,600 (GRCm38)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,065,136 (GRCm38)	nonsense	probably null
R5275:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,104,774 (GRCm38)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,073,904 (GRCm38)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,100,465 (GRCm38)	missense	probably benign
R5778:Fryl	UTSW	5	73,072,778 (GRCm38)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,090,755 (GRCm38)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,090,717 (GRCm38)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,097,372 (GRCm38)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,083,295 (GRCm38)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,099,997 (GRCm38)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,118,551 (GRCm38)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,112,788 (GRCm38)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,065,481 (GRCm38)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,191,759 (GRCm38)	intron	probably benign
R6310:Fryl	UTSW	5	73,191,761 (GRCm38)	intron	probably benign
R6429:Fryl	UTSW	5	73,090,751 (GRCm38)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,059,516 (GRCm38)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,133,312 (GRCm38)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,132,481 (GRCm38)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,054,781 (GRCm38)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,022,232 (GRCm38)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,065,094 (GRCm38)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,065,217 (GRCm38)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,065,032 (GRCm38)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,068,803 (GRCm38)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,022,142 (GRCm38)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,022,211 (GRCm38)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,073,929 (GRCm38)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,050,430 (GRCm38)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,055,608 (GRCm38)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,090,756 (GRCm38)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,073,908 (GRCm38)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,122,310 (GRCm38)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,065,095 (GRCm38)	missense	probably benign
R7236:Fryl	UTSW	5	73,108,478 (GRCm38)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,047,496 (GRCm38)	splice site	probably null
R7425:Fryl	UTSW	5	73,104,748 (GRCm38)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,023,988 (GRCm38)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,097,561 (GRCm38)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,098,196 (GRCm38)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,022,676 (GRCm38)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,081,039 (GRCm38)	missense	probably benign
R7548:Fryl	UTSW	5	73,191,762 (GRCm38)	missense	unknown
R7565:Fryl	UTSW	5	73,033,720 (GRCm38)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,088,396 (GRCm38)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,022,500 (GRCm38)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,110,245 (GRCm38)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,083,384 (GRCm38)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,071,298 (GRCm38)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,054,532 (GRCm38)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,101,807 (GRCm38)	splice site	probably null
R8110:Fryl	UTSW	5	73,133,277 (GRCm38)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,071,184 (GRCm38)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,050,339 (GRCm38)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,100,500 (GRCm38)	splice site	probably null
R8263:Fryl	UTSW	5	73,081,005 (GRCm38)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8359:Fryl	UTSW	5	73,075,933 (GRCm38)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,136,320 (GRCm38)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,118,447 (GRCm38)	makesense	probably null
R8450:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8514:Fryl	UTSW	5	73,085,356 (GRCm38)	missense	probably benign
R8536:Fryl	UTSW	5	73,100,353 (GRCm38)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,090,654 (GRCm38)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,132,562 (GRCm38)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,068,842 (GRCm38)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,024,775 (GRCm38)	missense
R9063:Fryl	UTSW	5	73,081,003 (GRCm38)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,108,577 (GRCm38)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,191,519 (GRCm38)	intron	probably benign
R9345:Fryl	UTSW	5	73,050,411 (GRCm38)	missense	probably benign
R9381:Fryl	UTSW	5	73,083,294 (GRCm38)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,191,809 (GRCm38)	missense	unknown
R9401:Fryl	UTSW	5	73,065,220 (GRCm38)	nonsense	probably null
R9497:Fryl	UTSW	5	73,057,791 (GRCm38)	missense
R9514:Fryl	UTSW	5	73,104,772 (GRCm38)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,022,155 (GRCm38)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,118,458 (GRCm38)	missense	probably benign
R9665:Fryl	UTSW	5	73,064,956 (GRCm38)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,059,536 (GRCm38)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,035,059 (GRCm38)	missense	probably benign
Z1088:Fryl	UTSW	5	73,090,738 (GRCm38)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,090,709 (GRCm38)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,072,837 (GRCm38)	missense	probably benign
Z1177:Fryl	UTSW	5	73,041,595 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- TACACTGTGCGGTGTCAGAC -3'
(R):5'- GTGTTAATAACCTTTCTGCCTTGG -3'

Sequencing Primer
(F):5'- TGTCAGACAGTGTCTAGGAAGCTG -3'
(R):5'- GCCTTGGTCTCCCTAGAAAATGTG -3'

Posted On

2016-06-06