Mutagenetix > Incidental Mutation

Incidental Mutation 'R5018:Vmn2r3'

389043

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r3

Ensembl Gene

ENSMUSG00000091572

Gene Name

vomeronasal 2, receptor 3

Synonyms

EG637004

MMRRC Submission

042609-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5018 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64166225-64197130 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 64178774 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 497 (E497G)

Ref Sequence

ENSEMBL: ENSMUSP00000134891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170244] [ENSMUST00000176328]

AlphaFold

H3BJ88

Predicted Effect

probably benign
Transcript: ENSMUST00000170244
AA Change: E469G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000126165
Gene: ENSMUSG00000091572
AA Change: E469G

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	64	479	4e-64	PFAM
Pfam:NCD3G	521	574	1.1e-17	PFAM
Pfam:7tm_3	605	842	2.9e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000176328
AA Change: E497G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000134891
Gene: ENSMUSG00000091572
AA Change: E497G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	92	507	9.5e-66	PFAM
Pfam:NCD3G	549	602	8.8e-17	PFAM
Pfam:7tm_3	635	869	8.5e-48	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.8%

Validation Efficiency

98% (49/50)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamdec1	A	T	14: 68,809,228 (GRCm39)	F245I	probably damaging	Het
Agbl5	G	A	5: 31,060,403 (GRCm39)	R141Q	probably damaging	Het
Alx4	G	T	2: 93,507,764 (GRCm39)	G353V	probably damaging	Het
Apol7b	A	G	15: 77,308,916 (GRCm39)	F61L	probably benign	Het
Aunip	T	A	4: 134,250,928 (GRCm39)		probably null	Het
Bfsp1	C	A	2: 143,704,802 (GRCm39)	R17L	possibly damaging	Het
Birc6	T	A	17: 74,947,054 (GRCm39)	D2926E	probably damaging	Het
Dmwd	A	G	7: 18,812,044 (GRCm39)	D166G	probably damaging	Het
Dnah10	T	C	5: 124,839,260 (GRCm39)	S1233P	possibly damaging	Het
Dnah11	G	T	12: 118,094,463 (GRCm39)	N868K	probably benign	Het
Eea1	T	C	10: 95,846,899 (GRCm39)	V393A	probably benign	Het
Fchsd1	C	T	18: 38,092,926 (GRCm39)		probably benign	Het
Flacc1	A	G	1: 58,730,109 (GRCm39)	V67A	probably benign	Het
Fyttd1	A	G	16: 32,722,787 (GRCm39)		probably null	Het
Hal	T	C	10: 93,343,413 (GRCm39)		probably null	Het
Hhat	A	T	1: 192,277,346 (GRCm39)	L371Q	probably damaging	Het
Hpse2	A	G	19: 43,373,263 (GRCm39)	F122S	possibly damaging	Het
Kif21b	A	G	1: 136,099,972 (GRCm39)	I1509V	probably benign	Het
Klhl20	T	C	1: 160,929,156 (GRCm39)	D334G	probably damaging	Het
Macf1	C	T	4: 123,279,392 (GRCm39)	D3870N	probably damaging	Het
Nlrc5	C	T	8: 95,252,080 (GRCm39)	A1867V	probably damaging	Het
Nr1h5	A	G	3: 102,855,111 (GRCm39)	L330P	probably damaging	Het
Or10g3b	A	C	14: 52,586,736 (GRCm39)	C256G	possibly damaging	Het
Pcdh15	T	G	10: 74,479,607 (GRCm39)	S573A	possibly damaging	Het
Polr1c	G	T	17: 46,558,635 (GRCm39)		probably benign	Het
Scd4	T	A	19: 44,326,048 (GRCm39)	M134K	probably benign	Het
Sh3gl2	A	G	4: 85,309,291 (GRCm39)		probably benign	Het
Sin3a	T	A	9: 57,018,175 (GRCm39)	S865T	probably benign	Het
Slitrk3	G	A	3: 72,957,845 (GRCm39)	T309I	probably benign	Het
Sspo	G	A	6: 48,432,634 (GRCm39)	E837K	probably damaging	Het
Stag1	A	G	9: 100,833,672 (GRCm39)	D1095G	probably benign	Het
Trat1	A	T	16: 48,555,168 (GRCm39)	L188*	probably null	Het
Ubn1	A	G	16: 4,881,589 (GRCm39)	D207G	probably damaging	Het
Ugp2	A	G	11: 21,281,052 (GRCm39)	Y219H	probably damaging	Het
Ugt2b1	G	T	5: 87,073,821 (GRCm39)	Y179*	probably null	Het
Vmn2r27	T	A	6: 124,201,141 (GRCm39)	D272V	probably benign	Het
Vmn2r91	G	T	17: 18,356,700 (GRCm39)	C789F	probably damaging	Het
Zfp276	A	G	8: 123,991,716 (GRCm39)		probably benign	Het

Other mutations in Vmn2r3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r3	APN	3	64,167,304 (GRCm39)	missense	probably damaging	1.00
IGL01468:Vmn2r3	APN	3	64,182,382 (GRCm39)	missense	possibly damaging	0.57
IGL02032:Vmn2r3	APN	3	64,182,476 (GRCm39)	missense	possibly damaging	0.95
IGL02405:Vmn2r3	APN	3	64,178,620 (GRCm39)	splice site	probably benign
IGL02640:Vmn2r3	APN	3	64,194,816 (GRCm39)	missense	probably benign
IGL02719:Vmn2r3	APN	3	64,183,031 (GRCm39)	missense	probably damaging	1.00
IGL02746:Vmn2r3	APN	3	64,167,239 (GRCm39)	missense	possibly damaging	0.74
IGL02952:Vmn2r3	APN	3	64,186,256 (GRCm39)	missense	probably damaging	1.00
IGL03390:Vmn2r3	APN	3	64,182,767 (GRCm39)	missense	possibly damaging	0.55
G1citation:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R0023:Vmn2r3	UTSW	3	64,182,787 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r3	UTSW	3	64,183,054 (GRCm39)	missense	possibly damaging	0.51
R0647:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R1071:Vmn2r3	UTSW	3	64,182,697 (GRCm39)	missense	possibly damaging	0.79
R1536:Vmn2r3	UTSW	3	64,182,538 (GRCm39)	missense	probably damaging	1.00
R1806:Vmn2r3	UTSW	3	64,194,810 (GRCm39)	missense	possibly damaging	0.73
R1806:Vmn2r3	UTSW	3	64,182,893 (GRCm39)	missense	probably benign	0.03
R1852:Vmn2r3	UTSW	3	64,166,815 (GRCm39)	missense	probably damaging	1.00
R1868:Vmn2r3	UTSW	3	64,166,537 (GRCm39)	missense	probably damaging	1.00
R2072:Vmn2r3	UTSW	3	64,182,493 (GRCm39)	missense	possibly damaging	0.87
R2240:Vmn2r3	UTSW	3	64,166,483 (GRCm39)	missense	probably benign	0.44
R2446:Vmn2r3	UTSW	3	64,182,733 (GRCm39)	missense	probably damaging	0.98
R4133:Vmn2r3	UTSW	3	64,183,138 (GRCm39)	missense	probably damaging	0.99
R4159:Vmn2r3	UTSW	3	64,194,850 (GRCm39)	nonsense	probably null
R4494:Vmn2r3	UTSW	3	64,182,692 (GRCm39)	missense	probably damaging	1.00
R4860:Vmn2r3	UTSW	3	64,183,022 (GRCm39)	missense	probably benign	0.00
R4895:Vmn2r3	UTSW	3	64,167,182 (GRCm39)	missense	probably benign	0.00
R4912:Vmn2r3	UTSW	3	64,166,618 (GRCm39)	missense	probably damaging	1.00
R5033:Vmn2r3	UTSW	3	64,167,220 (GRCm39)	missense	probably benign	0.09
R5126:Vmn2r3	UTSW	3	64,166,740 (GRCm39)	missense	probably damaging	1.00
R5148:Vmn2r3	UTSW	3	64,186,247 (GRCm39)	missense	probably damaging	1.00
R5414:Vmn2r3	UTSW	3	64,166,978 (GRCm39)	nonsense	probably null
R5785:Vmn2r3	UTSW	3	64,166,444 (GRCm39)	missense	possibly damaging	0.89
R5905:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R5992:Vmn2r3	UTSW	3	64,167,068 (GRCm39)	missense	probably damaging	1.00
R6028:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R6331:Vmn2r3	UTSW	3	64,186,182 (GRCm39)	missense	probably damaging	1.00
R6378:Vmn2r3	UTSW	3	64,182,517 (GRCm39)	missense	probably damaging	1.00
R6775:Vmn2r3	UTSW	3	64,183,039 (GRCm39)	missense	possibly damaging	0.88
R6822:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R6826:Vmn2r3	UTSW	3	64,182,327 (GRCm39)	nonsense	probably null
R6886:Vmn2r3	UTSW	3	64,166,927 (GRCm39)	missense	probably damaging	1.00
R6971:Vmn2r3	UTSW	3	64,166,668 (GRCm39)	missense	probably damaging	0.99
R7154:Vmn2r3	UTSW	3	64,194,732 (GRCm39)	missense	probably benign	0.02
R7192:Vmn2r3	UTSW	3	64,167,364 (GRCm39)	missense	probably benign	0.24
R7282:Vmn2r3	UTSW	3	64,168,825 (GRCm39)	missense	possibly damaging	0.90
R7472:Vmn2r3	UTSW	3	64,182,953 (GRCm39)	missense	probably benign	0.00
R7563:Vmn2r3	UTSW	3	64,182,770 (GRCm39)	missense	possibly damaging	0.60
R7726:Vmn2r3	UTSW	3	64,182,939 (GRCm39)	nonsense	probably null
R7966:Vmn2r3	UTSW	3	64,186,235 (GRCm39)	missense	probably damaging	0.99
R8025:Vmn2r3	UTSW	3	64,182,871 (GRCm39)	missense	possibly damaging	0.91
R8050:Vmn2r3	UTSW	3	64,178,714 (GRCm39)	missense	probably damaging	0.99
R8300:Vmn2r3	UTSW	3	64,182,347 (GRCm39)	missense	probably benign	0.00
R8402:Vmn2r3	UTSW	3	64,178,617 (GRCm39)	splice site	probably benign
R8486:Vmn2r3	UTSW	3	64,186,370 (GRCm39)	missense	probably damaging	1.00
R8523:Vmn2r3	UTSW	3	64,182,311 (GRCm39)	missense	probably benign	0.03
R8678:Vmn2r3	UTSW	3	64,166,896 (GRCm39)	missense	possibly damaging	0.76
R8885:Vmn2r3	UTSW	3	64,182,383 (GRCm39)	missense	probably benign	0.00
R8886:Vmn2r3	UTSW	3	64,194,892 (GRCm39)	missense	possibly damaging	0.47
R8905:Vmn2r3	UTSW	3	64,166,695 (GRCm39)	missense	probably damaging	0.99
R8937:Vmn2r3	UTSW	3	64,166,673 (GRCm39)	missense	probably damaging	1.00
R8955:Vmn2r3	UTSW	3	64,168,803 (GRCm39)	missense	possibly damaging	0.56
R9172:Vmn2r3	UTSW	3	64,186,403 (GRCm39)	missense	possibly damaging	0.79
R9485:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R9575:Vmn2r3	UTSW	3	64,178,735 (GRCm39)	missense	probably benign	0.01
R9618:Vmn2r3	UTSW	3	64,178,724 (GRCm39)	missense	probably damaging	0.98
X0022:Vmn2r3	UTSW	3	64,182,389 (GRCm39)	missense	probably damaging	1.00
X0022:Vmn2r3	UTSW	3	64,178,669 (GRCm39)	missense	possibly damaging	0.62

Predicted Primers

PCR Primer
(F):5'- TGTCAGACCAACTAACCCTTG -3'
(R):5'- AGTGGCTTCAGGATATCAGAGC -3'

Sequencing Primer
(F):5'- AACCCTTGAAGATTCAGTGTTCC -3'
(R):5'- ATATCAGAGCTGGTAATAAAAAGAGC -3'

Posted On

2016-06-06