Incidental Mutation 'R5020:Mettl25'
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ID389156
Institutional Source Beutler Lab
Gene Symbol Mettl25
Ensembl Gene ENSMUSG00000036009
Gene Namemethyltransferase like 25
SynonymsBC067068
MMRRC Submission 042611-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.104) question?
Stock #R5020 (G1)
Quality Score225
Status Validated
Chromosome10
Chromosomal Location105763189-105841380 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 105826207 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Histidine at position 301 (Y301H)
Ref Sequence ENSEMBL: ENSMUSP00000038665 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000046638] [ENSMUST00000176040] [ENSMUST00000176924]
Predicted Effect possibly damaging
Transcript: ENSMUST00000046638
AA Change: Y301H

PolyPhen 2 Score 0.869 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000038665
Gene: ENSMUSG00000036009
AA Change: Y301H

DomainStartEndE-ValueType
Pfam:Methyltransf_32 149 413 1.9e-30 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000176040
Predicted Effect probably benign
Transcript: ENSMUST00000176924
Meta Mutation Damage Score 0.1795 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.1%
Validation Efficiency 99% (72/73)
Allele List at MGI
Other mutations in this stock
Total: 63 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1300017J02Rik T C 9: 103,282,502 D70G probably benign Het
Acadsb A T 7: 131,441,200 probably null Het
Actl6a A G 3: 32,720,507 I340V possibly damaging Het
Alppl2 C T 1: 87,089,709 V19I probably benign Het
Ankrd34c T C 9: 89,729,706 K194R probably benign Het
Anpep T A 7: 79,833,727 M672L probably benign Het
Arhgap31 T C 16: 38,603,076 D876G probably damaging Het
Arid2 T A 15: 96,371,988 N1327K probably damaging Het
Atp5d C A 10: 80,145,429 P101Q probably benign Het
Bcl7b G A 5: 135,171,163 probably null Het
Brca2 A T 5: 150,560,436 S2907C probably damaging Het
Ccser2 T C 14: 36,940,177 E350G probably benign Het
Cdh23 G T 10: 60,308,032 D2929E probably damaging Het
Clec2i T A 6: 128,893,658 V78E probably benign Het
Cpxm1 A T 2: 130,395,977 probably null Het
Crybg3 C T 16: 59,554,796 V2032I possibly damaging Het
Eif5b A T 1: 38,019,069 K151* probably null Het
Gemin7 T A 7: 19,565,423 E82D possibly damaging Het
Gm14496 A G 2: 181,991,359 E45G possibly damaging Het
Gm20767 T C 13: 120,155,116 F164L possibly damaging Het
Gm21915 T C 9: 40,670,670 S20P probably damaging Het
Gm8126 A C 14: 43,261,569 E165A probably damaging Het
Grin2b T C 6: 135,733,407 D1047G probably benign Het
Igkv4-80 A C 6: 69,016,665 S81A probably benign Het
Ikbke T A 1: 131,273,660 Y150F probably damaging Het
Itih5 T A 2: 10,240,504 probably null Het
Lrig2 T C 3: 104,457,901 Q645R possibly damaging Het
Map4 A G 9: 110,068,800 M712V probably benign Het
Mecom A G 3: 29,961,106 S823P probably damaging Het
Mga A T 2: 119,951,173 K2136* probably null Het
Mmp24 A G 2: 155,810,284 E304G probably benign Het
Mmp3 T A 9: 7,445,984 D29E probably benign Het
Nbas T A 12: 13,374,712 I984N probably damaging Het
Nedd9 A G 13: 41,315,794 Y628H probably damaging Het
Nlrp2 C T 7: 5,328,077 C440Y probably damaging Het
Olfr1279 T C 2: 111,306,292 V29A probably benign Het
Olfr414 T G 1: 174,430,671 L81R probably damaging Het
P4ha2 T C 11: 54,131,190 V513A probably damaging Het
Pcsk4 T C 10: 80,326,035 N124S probably benign Het
Phf12 T C 11: 78,023,796 F139S probably damaging Het
Phlpp2 T A 8: 109,940,082 L1081H probably damaging Het
Phospho2 T A 2: 69,795,979 F160I probably damaging Het
Plch2 T G 4: 155,007,083 D115A probably damaging Het
Plekho1 A G 3: 95,989,539 F215S probably damaging Het
Psma4 C A 9: 54,952,772 A47E probably damaging Het
Rasa4 A C 5: 136,101,299 Q303P probably damaging Het
Rbmxl2 C T 7: 107,210,207 P233L probably damaging Het
Reln A T 5: 22,034,638 L877H probably damaging Het
Rtel1 A G 2: 181,322,514 probably null Het
Sept12 T C 16: 4,993,756 D116G probably damaging Het
Son T A 16: 91,656,375 V670E probably damaging Het
Spag9 T A 11: 94,097,786 I544N probably benign Het
Spsb3 T G 17: 24,887,062 probably benign Het
Tgfbr3 T C 5: 107,214,970 T59A probably damaging Het
Tsc1 T A 2: 28,676,519 I649K probably damaging Het
Txndc9 T C 1: 37,995,712 D37G probably benign Het
Uba7 C T 9: 107,978,914 A496V probably benign Het
Usf3 T A 16: 44,215,526 I123N probably damaging Het
Vmn1r184 T A 7: 26,267,530 S234T possibly damaging Het
Vsig10l C A 7: 43,465,317 S314* probably null Het
Wdfy4 A G 14: 33,079,935 F1922S probably damaging Het
Zfp219 C T 14: 52,009,655 R5H probably damaging Het
Zfp946 G T 17: 22,455,603 C446F probably benign Het
Other mutations in Mettl25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00310:Mettl25 APN 10 105826434 missense probably benign
IGL00698:Mettl25 APN 10 105793340 missense probably null 0.31
IGL00766:Mettl25 APN 10 105779582 splice site probably benign
IGL01360:Mettl25 APN 10 105823197 missense probably damaging 1.00
IGL01954:Mettl25 APN 10 105823207 missense probably damaging 1.00
IGL02088:Mettl25 APN 10 105823250 missense probably damaging 1.00
IGL02623:Mettl25 APN 10 105826324 missense probably damaging 0.96
IGL03245:Mettl25 APN 10 105826497 missense possibly damaging 0.86
IGL03134:Mettl25 UTSW 10 105826027 nonsense probably null
R0238:Mettl25 UTSW 10 105826525 missense probably damaging 1.00
R0238:Mettl25 UTSW 10 105826525 missense probably damaging 1.00
R0239:Mettl25 UTSW 10 105826525 missense probably damaging 1.00
R0239:Mettl25 UTSW 10 105826525 missense probably damaging 1.00
R1297:Mettl25 UTSW 10 105823265 missense probably benign 0.11
R1482:Mettl25 UTSW 10 105826590 missense possibly damaging 0.61
R1526:Mettl25 UTSW 10 105832983 missense possibly damaging 0.56
R1542:Mettl25 UTSW 10 105826120 missense probably benign 0.04
R1589:Mettl25 UTSW 10 105779632 missense probably damaging 1.00
R1901:Mettl25 UTSW 10 105826087 missense probably damaging 0.96
R1902:Mettl25 UTSW 10 105826087 missense probably damaging 0.96
R2016:Mettl25 UTSW 10 105797306 missense probably benign 0.00
R2355:Mettl25 UTSW 10 105763455 missense probably benign 0.00
R2920:Mettl25 UTSW 10 105765177 intron probably null
R3149:Mettl25 UTSW 10 105826353 missense probably benign 0.00
R4547:Mettl25 UTSW 10 105826017 missense probably damaging 1.00
R4646:Mettl25 UTSW 10 105826555 missense probably damaging 1.00
R4652:Mettl25 UTSW 10 105779648 nonsense probably null
R5435:Mettl25 UTSW 10 105779586 critical splice donor site probably null
R6947:Mettl25 UTSW 10 105826192 missense probably benign 0.00
R7075:Mettl25 UTSW 10 105829924 missense possibly damaging 0.68
R7729:Mettl25 UTSW 10 105766010 missense probably benign 0.03
R8074:Mettl25 UTSW 10 105826080 missense probably benign 0.02
R8108:Mettl25 UTSW 10 105823179 missense possibly damaging 0.53
RF009:Mettl25 UTSW 10 105833239 intron probably benign
Z1176:Mettl25 UTSW 10 105826098 missense possibly damaging 0.95
Predicted Primers PCR Primer
(F):5'- GTCGAATCACCTCCAGATCTTC -3'
(R):5'- GCCCAGAGAGTTGAAAGTGC -3'

Sequencing Primer
(F):5'- GTCATGCAATTGTGAATCAGCAGTG -3'
(R):5'- CCCAGAGAGTTGAAAGTGCCAAAG -3'
Posted On2016-06-06