Mutagenetix > Incidental Mutation

Incidental Mutation 'R5033:Vmn2r3'

389338

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r3

Ensembl Gene

ENSMUSG00000091572

Gene Name

vomeronasal 2, receptor 3

Synonyms

EG637004

MMRRC Submission

042624-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5033 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

64258961-64287495 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 64259799 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 637 (V637A)

Ref Sequence

ENSEMBL: ENSMUSP00000134891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170244] [ENSMUST00000176328]

AlphaFold

H3BJ88

Predicted Effect

probably benign
Transcript: ENSMUST00000170244
AA Change: V609A

PolyPhen 2 Score 0.090 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000126165
Gene: ENSMUSG00000091572
AA Change: V609A

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	64	479	4e-64	PFAM
Pfam:NCD3G	521	574	1.1e-17	PFAM
Pfam:7tm_3	605	842	2.9e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000176328
AA Change: V637A

PolyPhen 2 Score 0.090 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000134891
Gene: ENSMUSG00000091572
AA Change: V637A

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	92	507	9.5e-66	PFAM
Pfam:NCD3G	549	602	8.8e-17	PFAM
Pfam:7tm_3	635	869	8.5e-48	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1300017J02Rik	A	G	9: 103,279,414	V149A	probably benign	Het
1700012B07Rik	G	T	11: 109,794,154	C172*	probably null	Het
4931406P16Rik	C	T	7: 34,245,812	G336S	probably benign	Het
9930111J21Rik1	G	A	11: 48,947,706	R685W	probably damaging	Het
Arhgap10	A	T	8: 77,382,757	L399H	probably damaging	Het
Arih2	AGCCG	AG	9: 108,611,660		probably benign	Het
Atp13a2	A	G	4: 141,000,821	H575R	possibly damaging	Het
Bbof1	G	A	12: 84,411,270		probably null	Het
Catsperg2	A	C	7: 29,710,134	Y545D	possibly damaging	Het
Cdc42bpa	T	A	1: 180,065,015	I248N	probably damaging	Het
Cdk11b	A	T	4: 155,648,825		probably benign	Het
Cfap46	C	A	7: 139,603,860	L90F	probably benign	Het
Cndp2	A	G	18: 84,670,829	L276P	possibly damaging	Het
Col3a1	A	C	1: 45,346,110	D129A	probably damaging	Het
Daam1	C	A	12: 71,946,520	H354Q	unknown	Het
Dnah5	T	A	15: 28,421,678	F3892Y	probably damaging	Het
Dnajb3	A	G	1: 88,205,023	V219A	possibly damaging	Het
Drd5	A	G	5: 38,320,201	H179R	probably damaging	Het
Dym	A	G	18: 75,119,161	N333D	possibly damaging	Het
Eif2b3	A	G	4: 117,052,736	D156G	probably damaging	Het
Emc1	A	G	4: 139,371,696	D830G	probably damaging	Het
F13a1	A	C	13: 36,988,856	I183M	probably damaging	Het
Fam136b-ps	T	A	15: 31,276,897		probably benign	Het
Fdft1	C	A	14: 63,163,404	K93N	probably damaging	Het
Gcc2	A	G	10: 58,278,806	D959G	probably damaging	Het
Gm14085	T	A	2: 122,522,914		probably null	Het
Gm853	C	T	4: 130,221,615		probably null	Het
Gp2	A	G	7: 119,454,291	V149A	probably damaging	Het
Gpc1	G	A	1: 92,857,029	G308D	probably damaging	Het
Hdac9	A	T	12: 34,373,907	C587S	probably benign	Het
Hspg2	C	T	4: 137,518,940	R1010C	probably damaging	Het
Iars2	A	G	1: 185,317,928	V453A	possibly damaging	Het
Il1r1	T	G	1: 40,293,524	C121W	probably damaging	Het
Jazf1	A	G	6: 52,777,540	Y184H	probably damaging	Het
Kel	A	T	6: 41,699,055	Y234N	probably damaging	Het
Khk	G	A	5: 30,929,660	G186D	probably damaging	Het
Klf4	A	T	4: 55,530,301	M270K	probably benign	Het
Kmt2c	G	A	5: 25,314,708	L2135F	probably benign	Het
Krtap15	A	G	16: 88,829,156	Y37C	probably damaging	Het
Lars	A	G	18: 42,214,776	F994S	possibly damaging	Het
Lrp8	T	C	4: 107,834,755		probably null	Het
Lrrc2	A	T	9: 110,980,919	D341V	probably damaging	Het
Map4k4	A	G	1: 40,007,502	K646R	probably damaging	Het
Mrps9	T	A	1: 42,895,331		probably null	Het
Olfr1130	T	A	2: 87,607,711	C108S	probably damaging	Het
Olfr745	T	A	14: 50,643,162	Y288N	probably damaging	Het
Olfr898	C	T	9: 38,349,628	H176Y	probably damaging	Het
Pcnt	A	T	10: 76,399,945	C1401S	possibly damaging	Het
Pdgfrb	A	T	18: 61,077,668	K749M	probably damaging	Het
Pigo	A	T	4: 43,019,412	W969R	probably null	Het
Pigr	A	T	1: 130,844,699	Y218F	probably damaging	Het
Pih1d1	A	G	7: 45,154,854		probably benign	Het
Pik3cg	A	T	12: 32,199,196		probably null	Het
Pik3r6	A	T	11: 68,533,468	K300*	probably null	Het
Pip5k1c	A	G	10: 81,305,250	D64G	probably damaging	Het
Piwil2	A	T	14: 70,421,593	I161K	possibly damaging	Het
Pou3f1	A	G	4: 124,658,656	E317G	probably damaging	Het
Prdm8	C	T	5: 98,185,212	Q213*	probably null	Het
Prpf6	T	A	2: 181,650,106	C739S	possibly damaging	Het
Psmc3	T	A	2: 91,054,608	S40T	probably benign	Het
Psmd3	A	T	11: 98,682,824	D32V	probably damaging	Het
Ptpro	T	A	6: 137,443,594	V1007D	probably damaging	Het
Rapgef6	A	T	11: 54,691,381	H1373L	possibly damaging	Het
Rnf207	A	C	4: 152,313,209	S328A	probably benign	Het
Ros1	C	T	10: 52,128,416		probably null	Het
Ryr2	T	A	13: 11,587,254	I4581F	possibly damaging	Het
Sacm1l	C	T	9: 123,586,399	R480W	probably damaging	Het
Sec16a	A	G	2: 26,419,649	V664A	probably benign	Het
Selenoo	T	A	15: 89,092,766	M207K	probably damaging	Het
Sh3tc2	G	A	18: 62,014,891		probably null	Het
Slco2b1	G	T	7: 99,660,049	Q692K	probably benign	Het
Slfn4	A	T	11: 83,186,797	D137V	probably damaging	Het
Srrm2	G	A	17: 23,820,618	A2175T	probably damaging	Het
Stard9	T	A	2: 120,693,399	C692S	probably benign	Het
Sulf2	T	A	2: 166,081,622	D559V	probably benign	Het
Tbpl2	T	C	2: 24,087,158	I283V	probably benign	Het
Tmem19	A	G	10: 115,359,761	F46S	probably benign	Het
Trp63	T	A	16: 25,763,306	Y35N	probably damaging	Het
Ttn	T	A	2: 76,886,442		probably benign	Het
Ube2z	A	T	11: 96,050,322	H331Q	probably benign	Het
Ubr1	C	T	2: 120,911,997		probably null	Het
Uhrf1bp1	A	G	17: 27,886,864	E788G	probably damaging	Het
Vmn1r235	A	T	17: 21,262,288	I292F	possibly damaging	Het
Vmn2r1	T	A	3: 64,105,080	D787E	probably damaging	Het
Xpnpep1	C	A	19: 53,006,175	V341L	probably benign	Het
Yme1l1	A	G	2: 23,194,747	D619G	probably damaging	Het
Zfp597	G	A	16: 3,866,638	P85S	probably damaging	Het
Zscan20	A	G	4: 128,586,128	S857P	probably benign	Het

Other mutations in Vmn2r3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r3	APN	3	64259883	missense	probably damaging	1.00
IGL01468:Vmn2r3	APN	3	64274961	missense	possibly damaging	0.57
IGL02032:Vmn2r3	APN	3	64275055	missense	possibly damaging	0.95
IGL02405:Vmn2r3	APN	3	64271199	splice site	probably benign
IGL02640:Vmn2r3	APN	3	64287395	missense	probably benign
IGL02719:Vmn2r3	APN	3	64275610	missense	probably damaging	1.00
IGL02746:Vmn2r3	APN	3	64259818	missense	possibly damaging	0.74
IGL02952:Vmn2r3	APN	3	64278835	missense	probably damaging	1.00
IGL03390:Vmn2r3	APN	3	64275346	missense	possibly damaging	0.55
G1citation:Vmn2r3	UTSW	3	64287455	missense	probably benign	0.30
R0023:Vmn2r3	UTSW	3	64275366	missense	probably damaging	0.99
R0433:Vmn2r3	UTSW	3	64275633	missense	possibly damaging	0.51
R0647:Vmn2r3	UTSW	3	64275625	missense	probably damaging	1.00
R1071:Vmn2r3	UTSW	3	64275276	missense	possibly damaging	0.79
R1536:Vmn2r3	UTSW	3	64275117	missense	probably damaging	1.00
R1806:Vmn2r3	UTSW	3	64275472	missense	probably benign	0.03
R1806:Vmn2r3	UTSW	3	64287389	missense	possibly damaging	0.73
R1852:Vmn2r3	UTSW	3	64259394	missense	probably damaging	1.00
R1868:Vmn2r3	UTSW	3	64259116	missense	probably damaging	1.00
R2072:Vmn2r3	UTSW	3	64275072	missense	possibly damaging	0.87
R2240:Vmn2r3	UTSW	3	64259062	missense	probably benign	0.44
R2446:Vmn2r3	UTSW	3	64275312	missense	probably damaging	0.98
R4133:Vmn2r3	UTSW	3	64275717	missense	probably damaging	0.99
R4159:Vmn2r3	UTSW	3	64287429	nonsense	probably null
R4494:Vmn2r3	UTSW	3	64275271	missense	probably damaging	1.00
R4860:Vmn2r3	UTSW	3	64275601	missense	probably benign	0.00
R4895:Vmn2r3	UTSW	3	64259761	missense	probably benign	0.00
R4912:Vmn2r3	UTSW	3	64259197	missense	probably damaging	1.00
R5018:Vmn2r3	UTSW	3	64271353	missense	probably benign
R5126:Vmn2r3	UTSW	3	64259319	missense	probably damaging	1.00
R5148:Vmn2r3	UTSW	3	64278826	missense	probably damaging	1.00
R5414:Vmn2r3	UTSW	3	64259557	nonsense	probably null
R5785:Vmn2r3	UTSW	3	64259023	missense	possibly damaging	0.89
R5905:Vmn2r3	UTSW	3	64275277	missense	probably benign	0.19
R5992:Vmn2r3	UTSW	3	64259647	missense	probably damaging	1.00
R6028:Vmn2r3	UTSW	3	64275277	missense	probably benign	0.19
R6331:Vmn2r3	UTSW	3	64278761	missense	probably damaging	1.00
R6378:Vmn2r3	UTSW	3	64275096	missense	probably damaging	1.00
R6775:Vmn2r3	UTSW	3	64275618	missense	possibly damaging	0.88
R6822:Vmn2r3	UTSW	3	64287455	missense	probably benign	0.30
R6826:Vmn2r3	UTSW	3	64274906	nonsense	probably null
R6886:Vmn2r3	UTSW	3	64259506	missense	probably damaging	1.00
R6971:Vmn2r3	UTSW	3	64259247	missense	probably damaging	0.99
R7154:Vmn2r3	UTSW	3	64287311	missense	probably benign	0.02
R7192:Vmn2r3	UTSW	3	64259943	missense	probably benign	0.24
R7282:Vmn2r3	UTSW	3	64261404	missense	possibly damaging	0.90
R7472:Vmn2r3	UTSW	3	64275532	missense	probably benign	0.00
R7563:Vmn2r3	UTSW	3	64275349	missense	possibly damaging	0.60
R7726:Vmn2r3	UTSW	3	64275518	nonsense	probably null
R7966:Vmn2r3	UTSW	3	64278814	missense	probably damaging	0.99
R8025:Vmn2r3	UTSW	3	64275450	missense	possibly damaging	0.91
R8050:Vmn2r3	UTSW	3	64271293	missense	probably damaging	0.99
R8300:Vmn2r3	UTSW	3	64274926	missense	probably benign	0.00
R8402:Vmn2r3	UTSW	3	64271196	splice site	probably benign
R8486:Vmn2r3	UTSW	3	64278949	missense	probably damaging	1.00
R8523:Vmn2r3	UTSW	3	64274890	missense	probably benign	0.03
R8678:Vmn2r3	UTSW	3	64259475	missense	possibly damaging	0.76
R8885:Vmn2r3	UTSW	3	64274962	missense	probably benign	0.00
R8886:Vmn2r3	UTSW	3	64287471	missense	possibly damaging	0.47
R8905:Vmn2r3	UTSW	3	64259274	missense	probably damaging	0.99
R8937:Vmn2r3	UTSW	3	64259252	missense	probably damaging	1.00
R8955:Vmn2r3	UTSW	3	64261382	missense	possibly damaging	0.56
R9172:Vmn2r3	UTSW	3	64278982	missense	possibly damaging	0.79
R9485:Vmn2r3	UTSW	3	64275625	missense	probably damaging	1.00
R9575:Vmn2r3	UTSW	3	64271314	missense	probably benign	0.01
R9618:Vmn2r3	UTSW	3	64271303	missense	probably damaging	0.98
X0022:Vmn2r3	UTSW	3	64271248	missense	possibly damaging	0.62
X0022:Vmn2r3	UTSW	3	64274968	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TACGAAAACTGGGGTGCATG -3'
(R):5'- ACTGCCTCAGACTGTTCATAG -3'

Sequencing Primer
(F):5'- CAGTGCTAGAGTGACCTGGC -3'
(R):5'- GCCTCAGACTGTTCATAGTTAAATGG -3'

Posted On

2016-06-06