Mutagenetix > Incidental Mutation

Incidental Mutation 'R0433:Cfap54'

38939

Institutional Source

Beutler Lab

Gene Symbol

Cfap54

Ensembl Gene

ENSMUSG00000020014

Gene Name

cilia and flagella associated protein 54

Synonyms

LOC380653, Gm872, 4930485B16Rik

MMRRC Submission

038635-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R0433 (G1)

Quality Score

186

Status

Validated

Chromosome

Chromosomal Location

92775619-93081618 bp(-) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 92979080 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000148636 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168110] [ENSMUST00000170065] [ENSMUST00000212902]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000168110

SMART Domains

Protein: ENSMUSP00000129517
Gene: ENSMUSG00000020014

Domain	Start	End	E-Value	Type
low complexity region	3	37	N/A	INTRINSIC
low complexity region	39	48	N/A	INTRINSIC
Pfam:DUF4486	104	642	1.1e-269	PFAM
low complexity region	842	851	N/A	INTRINSIC
low complexity region	902	915	N/A	INTRINSIC
Blast:FN3	916	1002	4e-48	BLAST
low complexity region	1409	1426	N/A	INTRINSIC
low complexity region	1974	1984	N/A	INTRINSIC
low complexity region	2354	2370	N/A	INTRINSIC
low complexity region	2500	2513	N/A	INTRINSIC
low complexity region	2605	2616	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000170065

Predicted Effect

probably benign
Transcript: ENSMUST00000212902

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 94.3%

Validation Efficiency

99% (108/109)

MGI Phenotype

PHENOTYPE: Homozygous inactivation of this gene causes background-dependent lethality and hydroencephaly, male sterility associated with defects in spermiogenesis, and impaired mucociliary clearance. Airway epithelial cilia show structural defects and a decrease in ciliary beat frequency and cilia-driven flow. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 102 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110032F04Rik	T	C	3: 68,870,303 (GRCm38)	V199A	possibly damaging	Het
2410089E03Rik	T	C	15: 8,216,562 (GRCm38)	S1473P	probably benign	Het
Abcb5	T	C	12: 118,877,810 (GRCm38)	M967V	probably benign	Het
Adcy10	T	A	1: 165,552,022 (GRCm38)	L951Q	probably damaging	Het
Amer2	A	T	14: 60,378,583 (GRCm38)	S76C	probably damaging	Het
Atad1	T	C	19: 32,698,477 (GRCm38)	I182M	probably benign	Het
Bpi	A	G	2: 158,258,419 (GRCm38)	D42G	probably damaging	Het
C7	G	T	15: 4,988,916 (GRCm38)	T815K	probably damaging	Het
Cacna1g	T	A	11: 94,459,207 (GRCm38)	D604V	probably benign	Het
Camk1g	A	G	1: 193,354,058 (GRCm38)	F165L	probably damaging	Het
Ccdc69	C	T	11: 55,052,890 (GRCm38)		probably null	Het
Ccser2	A	C	14: 36,918,529 (GRCm38)	F37L	probably damaging	Het
Cfap43	A	G	19: 47,825,771 (GRCm38)	F208S	probably benign	Het
Cfap69	A	C	5: 5,649,853 (GRCm38)	D62E	probably damaging	Het
Cnksr2	A	T	X: 157,888,558 (GRCm38)	M483K	probably benign	Het
Cnksr2	C	A	X: 157,888,557 (GRCm38)	M483I	probably benign	Het
Cog8	T	C	8: 107,056,478 (GRCm38)	S60G	possibly damaging	Het
Col4a3	C	T	1: 82,670,219 (GRCm38)	P484S	unknown	Het
Col6a4	C	T	9: 106,067,994 (GRCm38)	G974R	probably damaging	Het
Dbnl	T	G	11: 5,796,825 (GRCm38)		probably null	Het
Dhcr7	T	C	7: 143,840,463 (GRCm38)	C114R	possibly damaging	Het
Dnah2	C	A	11: 69,459,288 (GRCm38)	D2340Y	probably damaging	Het
Dusp10	T	A	1: 184,069,196 (GRCm38)	Y387N	probably damaging	Het
Eipr1	C	T	12: 28,859,331 (GRCm38)	T199I	possibly damaging	Het
Emc2	T	G	15: 43,497,124 (GRCm38)		probably null	Het
Enpp3	A	G	10: 24,820,597 (GRCm38)	S147P	probably benign	Het
Fam133b	T	A	5: 3,558,560 (GRCm38)		probably benign	Het
Fam205c	A	G	4: 42,874,013 (GRCm38)		probably benign	Het
Fat1	C	A	8: 45,024,649 (GRCm38)	T2244K	possibly damaging	Het
Fbn1	A	G	2: 125,348,215 (GRCm38)	S1453P	possibly damaging	Het
Fez2	A	T	17: 78,418,047 (GRCm38)	F13I	probably damaging	Het
Ggnbp2	T	C	11: 84,836,420 (GRCm38)	K530R	probably damaging	Het
Gm597	A	T	1: 28,777,342 (GRCm38)	Y536*	probably null	Het
Gpa33	T	C	1: 166,163,761 (GRCm38)		probably benign	Het
Gpr142	T	C	11: 114,805,997 (GRCm38)	I123T	probably damaging	Het
Il21	T	G	3: 37,232,535 (GRCm38)	I11L	possibly damaging	Het
Klhl7	A	G	5: 24,127,702 (GRCm38)	E86G	probably damaging	Het
Klk10	G	T	7: 43,781,565 (GRCm38)	A11S	possibly damaging	Het
Knl1	A	T	2: 119,104,061 (GRCm38)	D2115V	probably damaging	Het
Lonp2	A	G	8: 86,633,954 (GRCm38)	D185G	probably damaging	Het
Lrrc47	T	C	4: 154,018,365 (GRCm38)		probably benign	Het
Lrrcc1	A	G	3: 14,559,374 (GRCm38)	I698V	probably damaging	Het
Lzts2	T	C	19: 45,021,676 (GRCm38)	V83A	possibly damaging	Het
Melk	C	A	4: 44,340,614 (GRCm38)		probably benign	Het
Mical1	G	A	10: 41,479,490 (GRCm38)	V150I	probably benign	Het
Morn3	C	A	5: 123,039,333 (GRCm38)	M129I	probably benign	Het
Mroh2b	T	A	15: 4,941,634 (GRCm38)	D1040E	probably benign	Het
Mroh5	T	C	15: 73,790,028 (GRCm38)	N438S	probably benign	Het
Mroh5	T	A	15: 73,790,808 (GRCm38)	Q387L	probably damaging	Het
Myh15	A	G	16: 49,145,236 (GRCm38)	D1168G	probably damaging	Het
Nek10	A	G	14: 14,860,927 (GRCm38)	E493G	probably benign	Het
Nipsnap3a	A	G	4: 53,000,316 (GRCm38)	Y227C	probably damaging	Het
Nlrp9c	T	A	7: 26,385,819 (GRCm38)	T112S	probably benign	Het
Nphp4	T	C	4: 152,518,172 (GRCm38)	V401A	probably benign	Het
Nr1h2	A	G	7: 44,549,987 (GRCm38)	*365Q	probably null	Het
Olfr1339	T	C	4: 118,735,090 (GRCm38)	V187A	probably benign	Het
Olfr474	T	C	7: 107,955,262 (GRCm38)	I207T	probably damaging	Het
Pacs2	T	A	12: 113,056,844 (GRCm38)	V279D	possibly damaging	Het
Pdcd2	C	T	17: 15,526,384 (GRCm38)	C171Y	probably benign	Het
Pde11a	T	A	2: 76,337,706 (GRCm38)	D301V	possibly damaging	Het
Pfpl	T	G	19: 12,429,475 (GRCm38)	N363K	probably damaging	Het
Phf14	T	A	6: 11,933,743 (GRCm38)	S201R	probably damaging	Het
Pip4k2c	G	A	10: 127,208,946 (GRCm38)	P66S	probably benign	Het
Pou2f3	G	T	9: 43,127,398 (GRCm38)	H392N	probably benign	Het
Pou3f1	G	T	4: 124,658,904 (GRCm38)	G400C	probably damaging	Het
Ptprg	T	C	14: 12,220,620 (GRCm38)	I1219T	probably damaging	Het
Rfx6	A	G	10: 51,720,028 (GRCm38)	D435G	probably damaging	Het
Rhpn2	T	A	7: 35,385,474 (GRCm38)	S598T	probably benign	Het
Sdccag8	C	A	1: 176,844,821 (GRCm38)		probably null	Het
Sec16b	C	A	1: 157,534,709 (GRCm38)	Y43*	probably null	Het
Sele	T	C	1: 164,049,244 (GRCm38)	Y30H	possibly damaging	Het
Sgsm2	C	T	11: 74,858,190 (GRCm38)		probably null	Het
Slc45a2	T	C	15: 11,025,745 (GRCm38)	Y394H	probably benign	Het
Slc4a10	T	G	2: 62,289,983 (GRCm38)	I788S	probably benign	Het
Slmap	A	T	14: 26,453,594 (GRCm38)	L161*	probably null	Het
Slx4	A	T	16: 3,986,018 (GRCm38)	D977E	probably benign	Het
Spen	A	T	4: 141,483,758 (GRCm38)	M608K	unknown	Het
St8sia4	G	C	1: 95,591,704 (GRCm38)	T353R	probably damaging	Het
Stab2	G	T	10: 86,843,491 (GRCm38)		probably benign	Het
Stx12	C	T	4: 132,858,430 (GRCm38)	G213D	probably damaging	Het
Synj2	A	T	17: 6,033,848 (GRCm38)	N270Y	probably damaging	Het
Tdrd9	C	T	12: 112,025,581 (GRCm38)	R438*	probably null	Het
Tert	T	C	13: 73,627,081 (GRCm38)	Y18H	probably damaging	Het
Tph1	A	T	7: 46,653,821 (GRCm38)	F244L	probably damaging	Het
Triobp	T	C	15: 78,968,201 (GRCm38)	F852L	possibly damaging	Het
Trpv1	T	C	11: 73,253,008 (GRCm38)		probably benign	Het
Uggt2	A	T	14: 119,075,329 (GRCm38)		probably null	Het
Ulk4	A	G	9: 121,044,819 (GRCm38)	I1182T	probably benign	Het
Uqcc1	A	G	2: 155,910,368 (GRCm38)	Y98H	probably damaging	Het
Usp25	A	G	16: 77,109,217 (GRCm38)	I854V	probably benign	Het
Usp50	T	C	2: 126,761,544 (GRCm38)	S361G	probably damaging	Het
Uspl1	C	A	5: 149,214,815 (GRCm38)	Q743K	probably damaging	Het
Vmn2r3	A	G	3: 64,275,633 (GRCm38)	V215A	possibly damaging	Het
Vmn2r61	A	T	7: 42,265,911 (GRCm38)	H94L	probably benign	Het
Vps37c	T	C	19: 10,713,029 (GRCm38)	V285A	probably benign	Het
Vwa8	T	C	14: 79,062,676 (GRCm38)	V983A	probably damaging	Het
Wdr78	T	C	4: 103,103,253 (GRCm38)	N67D	probably benign	Het
Zcchc9	C	T	13: 91,805,962 (GRCm38)	R58H	probably benign	Het
Zdbf2	T	C	1: 63,306,143 (GRCm38)	V1227A	possibly damaging	Het
Zfp292	T	C	4: 34,839,959 (GRCm38)	K64E	probably damaging	Het
Zfp948	A	G	17: 21,587,502 (GRCm38)	T319A	probably benign	Het
Zp3r	T	G	1: 130,577,133 (GRCm38)		probably benign	Het

Other mutations in Cfap54

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01329:Cfap54	APN	10	93,081,523 (GRCm38)	missense	unknown
IGL02034:Cfap54	APN	10	93,061,485 (GRCm38)	missense	probably damaging	0.99
IGL02082:Cfap54	APN	10	93,081,458 (GRCm38)	missense	unknown
IGL02434:Cfap54	APN	10	93,066,754 (GRCm38)	missense	probably benign	0.20
R0011:Cfap54	UTSW	10	93,065,225 (GRCm38)	missense	probably damaging	0.97
R0011:Cfap54	UTSW	10	93,065,225 (GRCm38)	missense	probably damaging	0.97
R0032:Cfap54	UTSW	10	92,932,697 (GRCm38)	missense	probably benign	0.04
R0032:Cfap54	UTSW	10	92,932,697 (GRCm38)	missense	probably benign	0.04
R0040:Cfap54	UTSW	10	92,977,039 (GRCm38)	missense	probably benign	0.33
R0044:Cfap54	UTSW	10	93,035,433 (GRCm38)	missense	probably null	0.46
R0086:Cfap54	UTSW	10	93,028,594 (GRCm38)	missense	possibly damaging	0.86
R0104:Cfap54	UTSW	10	93,028,652 (GRCm38)	missense	probably damaging	1.00
R0194:Cfap54	UTSW	10	93,034,662 (GRCm38)	unclassified	probably benign
R0234:Cfap54	UTSW	10	92,899,160 (GRCm38)	nonsense	probably null
R0308:Cfap54	UTSW	10	92,885,364 (GRCm38)	missense	unknown
R0332:Cfap54	UTSW	10	93,035,457 (GRCm38)	missense	probably damaging	1.00
R0409:Cfap54	UTSW	10	92,776,213 (GRCm38)	missense	probably benign	0.00
R0436:Cfap54	UTSW	10	93,038,975 (GRCm38)	missense	possibly damaging	0.95
R0463:Cfap54	UTSW	10	92,874,943 (GRCm38)	critical splice donor site	probably null
R0523:Cfap54	UTSW	10	92,908,883 (GRCm38)	utr 3 prime	probably benign
R0551:Cfap54	UTSW	10	93,025,122 (GRCm38)	missense	probably benign	0.35
R0595:Cfap54	UTSW	10	92,884,736 (GRCm38)	missense	unknown
R0617:Cfap54	UTSW	10	92,829,650 (GRCm38)	splice site	probably benign
R0632:Cfap54	UTSW	10	92,885,096 (GRCm38)	missense	unknown
R0730:Cfap54	UTSW	10	93,034,737 (GRCm38)	missense	probably benign	0.05
R0786:Cfap54	UTSW	10	92,967,535 (GRCm38)	missense	possibly damaging	0.72
R0883:Cfap54	UTSW	10	92,870,669 (GRCm38)	missense	unknown
R1004:Cfap54	UTSW	10	93,066,696 (GRCm38)	splice site	probably benign
R1033:Cfap54	UTSW	10	92,839,449 (GRCm38)	missense	probably benign	0.07
R1168:Cfap54	UTSW	10	92,937,920 (GRCm38)	missense	probably damaging	0.99
R1186:Cfap54	UTSW	10	92,875,994 (GRCm38)	missense	unknown
R1429:Cfap54	UTSW	10	92,821,038 (GRCm38)	missense	probably benign	0.01
R1443:Cfap54	UTSW	10	92,932,721 (GRCm38)	missense	probably damaging	1.00
R1467:Cfap54	UTSW	10	92,969,763 (GRCm38)	missense	probably benign	0.01
R1557:Cfap54	UTSW	10	92,984,227 (GRCm38)	missense	possibly damaging	0.68
R1687:Cfap54	UTSW	10	92,932,640 (GRCm38)	missense	probably damaging	1.00
R1690:Cfap54	UTSW	10	93,035,442 (GRCm38)	missense	possibly damaging	0.95
R1711:Cfap54	UTSW	10	93,011,020 (GRCm38)	missense	probably damaging	1.00
R1756:Cfap54	UTSW	10	93,048,061 (GRCm38)	missense	probably damaging	1.00
R1769:Cfap54	UTSW	10	92,904,263 (GRCm38)	critical splice donor site	probably null
R1835:Cfap54	UTSW	10	92,962,375 (GRCm38)	missense	probably benign	0.35
R1889:Cfap54	UTSW	10	93,034,710 (GRCm38)	missense	possibly damaging	0.94
R1915:Cfap54	UTSW	10	92,884,702 (GRCm38)	missense	unknown
R1958:Cfap54	UTSW	10	92,997,342 (GRCm38)	missense	probably benign	0.18
R2005:Cfap54	UTSW	10	92,884,768 (GRCm38)	missense	unknown
R2018:Cfap54	UTSW	10	93,016,604 (GRCm38)	missense	probably benign	0.00
R2045:Cfap54	UTSW	10	93,038,809 (GRCm38)	splice site	probably null
R2059:Cfap54	UTSW	10	92,942,979 (GRCm38)	unclassified	probably benign
R2100:Cfap54	UTSW	10	93,001,937 (GRCm38)	missense	possibly damaging	0.84
R2110:Cfap54	UTSW	10	92,886,367 (GRCm38)	missense	unknown
R2392:Cfap54	UTSW	10	93,025,011 (GRCm38)	critical splice donor site	probably null
R2508:Cfap54	UTSW	10	92,997,374 (GRCm38)	missense	possibly damaging	0.72
R2852:Cfap54	UTSW	10	92,940,155 (GRCm38)	missense	probably damaging	1.00
R2857:Cfap54	UTSW	10	93,045,282 (GRCm38)	missense	probably damaging	0.99
R2871:Cfap54	UTSW	10	92,921,419 (GRCm38)	missense	possibly damaging	0.86
R2871:Cfap54	UTSW	10	92,921,419 (GRCm38)	missense	possibly damaging	0.86
R3107:Cfap54	UTSW	10	92,994,683 (GRCm38)	missense	probably benign	0.04
R3108:Cfap54	UTSW	10	92,994,683 (GRCm38)	missense	probably benign	0.04
R3157:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3158:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3159:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3161:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3162:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3162:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3508:Cfap54	UTSW	10	92,885,424 (GRCm38)	missense	unknown
R3730:Cfap54	UTSW	10	93,011,473 (GRCm38)	nonsense	probably null
R3770:Cfap54	UTSW	10	92,878,536 (GRCm38)	missense	unknown
R3776:Cfap54	UTSW	10	93,045,100 (GRCm38)	missense	probably damaging	1.00
R3778:Cfap54	UTSW	10	92,904,344 (GRCm38)	utr 3 prime	probably benign
R3795:Cfap54	UTSW	10	92,942,873 (GRCm38)	unclassified	probably benign
R3834:Cfap54	UTSW	10	92,801,123 (GRCm38)	splice site	probably benign
R3891:Cfap54	UTSW	10	93,038,846 (GRCm38)	missense	possibly damaging	0.87
R3932:Cfap54	UTSW	10	92,829,757 (GRCm38)	missense	probably benign	0.03
R3973:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3974:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3976:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3978:Cfap54	UTSW	10	92,962,412 (GRCm38)	missense	probably benign	0.01
R4190:Cfap54	UTSW	10	92,885,023 (GRCm38)	missense	unknown
R4389:Cfap54	UTSW	10	92,967,500 (GRCm38)	missense	probably benign	0.37
R4542:Cfap54	UTSW	10	93,025,129 (GRCm38)	missense	probably benign	0.12
R4564:Cfap54	UTSW	10	92,839,540 (GRCm38)	unclassified	probably benign
R4576:Cfap54	UTSW	10	93,043,228 (GRCm38)	critical splice donor site	probably null
R4620:Cfap54	UTSW	10	92,969,757 (GRCm38)	missense	probably benign	0.01
R4714:Cfap54	UTSW	10	92,815,918 (GRCm38)	missense	probably benign	0.01
R4762:Cfap54	UTSW	10	93,061,453 (GRCm38)	splice site	probably null
R4776:Cfap54	UTSW	10	92,972,694 (GRCm38)	missense	possibly damaging	0.96
R4819:Cfap54	UTSW	10	92,836,477 (GRCm38)	nonsense	probably null
R4827:Cfap54	UTSW	10	92,902,075 (GRCm38)	utr 3 prime	probably benign
R4832:Cfap54	UTSW	10	92,967,528 (GRCm38)	missense	probably benign	0.01
R4965:Cfap54	UTSW	10	93,066,799 (GRCm38)	missense	probably benign	0.23
R5001:Cfap54	UTSW	10	92,964,534 (GRCm38)	missense	probably benign	0.01
R5060:Cfap54	UTSW	10	93,039,151 (GRCm38)	missense	probably damaging	1.00
R5067:Cfap54	UTSW	10	93,066,766 (GRCm38)	missense	probably benign	0.17
R5069:Cfap54	UTSW	10	92,937,774 (GRCm38)	missense	probably benign
R5094:Cfap54	UTSW	10	92,898,999 (GRCm38)	utr 3 prime	probably benign
R5109:Cfap54	UTSW	10	92,937,891 (GRCm38)	missense	probably benign	0.03
R5127:Cfap54	UTSW	10	92,886,387 (GRCm38)	splice site	probably null
R5143:Cfap54	UTSW	10	93,029,158 (GRCm38)	missense	possibly damaging	0.73
R5147:Cfap54	UTSW	10	92,937,838 (GRCm38)	missense	probably benign	0.00
R5158:Cfap54	UTSW	10	93,065,197 (GRCm38)	missense	probably damaging	1.00
R5256:Cfap54	UTSW	10	93,045,023 (GRCm38)	splice site	probably null
R5256:Cfap54	UTSW	10	92,935,091 (GRCm38)	nonsense	probably null
R5266:Cfap54	UTSW	10	92,815,902 (GRCm38)	missense	probably benign	0.16
R5304:Cfap54	UTSW	10	92,821,106 (GRCm38)	missense	probably damaging	0.97
R5369:Cfap54	UTSW	10	93,061,257 (GRCm38)	intron	probably benign
R5406:Cfap54	UTSW	10	93,001,858 (GRCm38)	missense	probably benign	0.33
R5471:Cfap54	UTSW	10	93,028,660 (GRCm38)	missense	probably damaging	1.00
R5485:Cfap54	UTSW	10	93,029,117 (GRCm38)	missense	probably damaging	1.00
R5540:Cfap54	UTSW	10	92,972,608 (GRCm38)	missense	possibly damaging	0.85
R5586:Cfap54	UTSW	10	92,972,611 (GRCm38)	nonsense	probably null
R5614:Cfap54	UTSW	10	93,045,049 (GRCm38)	missense	probably damaging	1.00
R5634:Cfap54	UTSW	10	92,904,263 (GRCm38)	critical splice donor site	probably benign
R5680:Cfap54	UTSW	10	92,979,017 (GRCm38)	nonsense	probably null
R5797:Cfap54	UTSW	10	92,967,576 (GRCm38)	missense	probably benign	0.11
R5859:Cfap54	UTSW	10	93,016,524 (GRCm38)	nonsense	probably null
R5878:Cfap54	UTSW	10	92,964,561 (GRCm38)	missense	probably benign	0.01
R5910:Cfap54	UTSW	10	93,065,181 (GRCm38)	missense	probably damaging	0.99
R5936:Cfap54	UTSW	10	92,962,412 (GRCm38)	missense	probably benign	0.01
R5994:Cfap54	UTSW	10	93,039,081 (GRCm38)	missense	probably damaging	0.99
R6080:Cfap54	UTSW	10	93,045,335 (GRCm38)	missense	possibly damaging	0.64
R6268:Cfap54	UTSW	10	93,038,909 (GRCm38)	missense	probably damaging	1.00
R6296:Cfap54	UTSW	10	93,066,846 (GRCm38)	missense	probably damaging	1.00
R6409:Cfap54	UTSW	10	92,967,492 (GRCm38)	missense	probably benign	0.04
R6545:Cfap54	UTSW	10	92,836,457 (GRCm38)	missense	probably benign	0.31
R6570:Cfap54	UTSW	10	92,815,958 (GRCm38)	missense	unknown
R6597:Cfap54	UTSW	10	92,999,040 (GRCm38)	missense	possibly damaging	0.85
R6702:Cfap54	UTSW	10	92,868,734 (GRCm38)	missense	unknown
R6703:Cfap54	UTSW	10	92,868,734 (GRCm38)	missense	unknown
R6720:Cfap54	UTSW	10	92,821,119 (GRCm38)	missense	probably benign	0.07
R6841:Cfap54	UTSW	10	92,875,015 (GRCm38)	missense	unknown
R6910:Cfap54	UTSW	10	92,836,512 (GRCm38)	missense	probably benign	0.29
R6953:Cfap54	UTSW	10	92,994,678 (GRCm38)	missense	probably benign	0.19
R7009:Cfap54	UTSW	10	92,875,019 (GRCm38)	missense	unknown
R7129:Cfap54	UTSW	10	93,016,571 (GRCm38)	missense	probably benign	0.06
R7131:Cfap54	UTSW	10	92,821,104 (GRCm38)	missense	probably benign	0.03
R7171:Cfap54	UTSW	10	92,776,210 (GRCm38)	missense	probably damaging	0.99
R7189:Cfap54	UTSW	10	92,937,728 (GRCm38)	missense	unknown
R7225:Cfap54	UTSW	10	92,904,374 (GRCm38)	missense	unknown
R7270:Cfap54	UTSW	10	92,839,458 (GRCm38)	missense	probably benign	0.03
R7323:Cfap54	UTSW	10	92,801,138 (GRCm38)	missense	probably benign	0.00
R7380:Cfap54	UTSW	10	93,047,978 (GRCm38)	missense	probably damaging	1.00
R7395:Cfap54	UTSW	10	92,884,703 (GRCm38)	missense	unknown
R7411:Cfap54	UTSW	10	92,868,755 (GRCm38)	missense	unknown
R7503:Cfap54	UTSW	10	92,887,436 (GRCm38)	splice site	probably null
R7622:Cfap54	UTSW	10	92,956,944 (GRCm38)	missense	unknown
R7679:Cfap54	UTSW	10	92,967,512 (GRCm38)	missense	probably benign	0.01
R7776:Cfap54	UTSW	10	92,868,741 (GRCm38)	missense	unknown
R7844:Cfap54	UTSW	10	92,902,058 (GRCm38)	missense	unknown
R7980:Cfap54	UTSW	10	92,982,060 (GRCm38)	missense	possibly damaging	0.95
R7988:Cfap54	UTSW	10	92,902,079 (GRCm38)	missense	unknown
R8101:Cfap54	UTSW	10	92,884,796 (GRCm38)	missense	unknown
R8119:Cfap54	UTSW	10	92,868,810 (GRCm38)	missense	unknown
R8134:Cfap54	UTSW	10	92,878,516 (GRCm38)	missense	unknown
R8168:Cfap54	UTSW	10	92,908,877 (GRCm38)	missense	unknown
R8179:Cfap54	UTSW	10	92,997,316 (GRCm38)	missense	possibly damaging	0.68
R8392:Cfap54	UTSW	10	92,962,417 (GRCm38)	missense	unknown
R8436:Cfap54	UTSW	10	92,964,536 (GRCm38)	missense	unknown
R8505:Cfap54	UTSW	10	92,978,993 (GRCm38)	missense	probably benign	0.03
R8671:Cfap54	UTSW	10	92,955,072 (GRCm38)	missense	unknown
R8716:Cfap54	UTSW	10	92,964,632 (GRCm38)	missense	probably benign	0.00
R8816:Cfap54	UTSW	10	92,878,592 (GRCm38)	missense	unknown
R8822:Cfap54	UTSW	10	93,039,141 (GRCm38)	missense	probably benign	0.09
R8827:Cfap54	UTSW	10	92,938,248 (GRCm38)	missense	unknown
R8920:Cfap54	UTSW	10	92,940,337 (GRCm38)	critical splice acceptor site	probably null
R8924:Cfap54	UTSW	10	93,001,823 (GRCm38)	missense	probably damaging	0.99
R8954:Cfap54	UTSW	10	93,043,393 (GRCm38)	missense	probably damaging	1.00
R8963:Cfap54	UTSW	10	93,028,700 (GRCm38)	nonsense	probably null
R9010:Cfap54	UTSW	10	92,899,059 (GRCm38)	missense	unknown
R9017:Cfap54	UTSW	10	92,816,021 (GRCm38)	missense	probably benign	0.07
R9093:Cfap54	UTSW	10	92,815,908 (GRCm38)	missense	probably benign	0.03
R9095:Cfap54	UTSW	10	93,011,020 (GRCm38)	missense	probably damaging	1.00
R9142:Cfap54	UTSW	10	92,984,235 (GRCm38)	missense	possibly damaging	0.87
R9178:Cfap54	UTSW	10	92,994,717 (GRCm38)	missense	probably benign	0.10
R9196:Cfap54	UTSW	10	93,037,891 (GRCm38)	missense	probably benign	0.22
R9203:Cfap54	UTSW	10	93,045,128 (GRCm38)	missense	probably benign	0.30
R9258:Cfap54	UTSW	10	92,935,098 (GRCm38)	missense	unknown
R9275:Cfap54	UTSW	10	93,039,186 (GRCm38)	missense	possibly damaging	0.86
R9287:Cfap54	UTSW	10	92,969,703 (GRCm38)	missense	possibly damaging	0.50
R9289:Cfap54	UTSW	10	92,821,074 (GRCm38)	missense	possibly damaging	0.83
R9310:Cfap54	UTSW	10	92,962,315 (GRCm38)	missense	unknown
R9397:Cfap54	UTSW	10	92,997,285 (GRCm38)	missense	probably damaging	0.96
R9462:Cfap54	UTSW	10	92,902,058 (GRCm38)	missense	unknown
R9697:Cfap54	UTSW	10	92,956,989 (GRCm38)	missense	unknown
R9746:Cfap54	UTSW	10	92,801,219 (GRCm38)	missense	probably benign	0.03
R9755:Cfap54	UTSW	10	92,921,368 (GRCm38)	missense	unknown
X0022:Cfap54	UTSW	10	92,932,614 (GRCm38)	missense	probably damaging	1.00
X0022:Cfap54	UTSW	10	92,878,603 (GRCm38)	missense	unknown
X0027:Cfap54	UTSW	10	93,001,888 (GRCm38)	missense	possibly damaging	0.86
X0027:Cfap54	UTSW	10	92,878,538 (GRCm38)	missense	unknown
Z1177:Cfap54	UTSW	10	92,979,026 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCCTTCTTCTCAGTGGATGGCAATG -3'
(R):5'- CAGCTAGAGCTTGTTAGTTGCCCC -3'

Sequencing Primer
(F):5'- ATGGCAATGGCGAGCAC -3'
(R):5'- GCCCCTTAAATTTTATGGGGAAG -3'

Posted On

2013-05-23