Mutagenetix > Incidental Mutation

Incidental Mutation 'R4999:Vmn2r99'

389774

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

042593-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R4999 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to G at 19362135 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 1 (M1V)

Ref Sequence

ENSEMBL: ENSMUSP00000156067 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably null
Transcript: ENSMUST00000176107
AA Change: M1V

PolyPhen 2 Score 0.889 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: M1V

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000231989
AA Change: M1V

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000232310

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.4%
20x: 92.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930444P10Rik	A	G	1: 16,068,798 (GRCm38)		probably null	Het
Abca4	T	A	3: 122,105,370 (GRCm38)	V667D	probably damaging	Het
Aco1	A	G	4: 40,176,507 (GRCm38)	I224V	probably damaging	Het
Arel1	C	T	12: 84,931,767 (GRCm38)	V364M	probably damaging	Het
Arhgap42	A	G	9: 9,009,434 (GRCm38)	V484A	probably damaging	Het
Asap2	T	C	12: 21,252,765 (GRCm38)	F681L	probably benign	Het
Atrn	C	A	2: 130,975,954 (GRCm38)	D809E	probably damaging	Het
BC037034	T	A	5: 138,261,622 (GRCm38)	T391S	probably damaging	Het
Ccdc70	G	A	8: 21,973,250 (GRCm38)	V19M	possibly damaging	Het
Ccp110	G	T	7: 118,730,012 (GRCm38)	E73*	probably null	Het
Cfap57	A	C	4: 118,595,848 (GRCm38)	S553A	probably benign	Het
Cftr	A	G	6: 18,221,614 (GRCm38)	K212E	probably benign	Het
Chkb	A	T	15: 89,428,165 (GRCm38)	Y216N	probably damaging	Het
Cntnap2	G	T	6: 45,920,834 (GRCm38)	D149Y	probably damaging	Het
Cpd	A	G	11: 76,846,222 (GRCm38)		probably null	Het
Creb3l1	C	T	2: 91,983,226 (GRCm38)	D489N	probably benign	Het
Crhbp	A	G	13: 95,442,245 (GRCm38)	F123L	probably damaging	Het
Cryab	T	C	9: 50,754,609 (GRCm38)	V100A	possibly damaging	Het
Csmd2	T	C	4: 128,521,930 (GRCm38)	I2684T	probably benign	Het
Ctbp2	G	T	7: 133,014,649 (GRCm38)	P186T	possibly damaging	Het
Ctnna2	T	C	6: 76,915,762 (GRCm38)	N814S	possibly damaging	Het
Dntt	T	C	19: 41,039,856 (GRCm38)	V197A	probably damaging	Het
Fam135a	G	A	1: 24,020,677 (GRCm38)	A1187V	possibly damaging	Het
Filip1l	A	T	16: 57,570,415 (GRCm38)	Q455H	probably benign	Het
Grm2	T	C	9: 106,653,990 (GRCm38)	E100G	probably damaging	Het
Gtf2ird2	G	A	5: 134,217,464 (GRCm38)	V855M	probably damaging	Het
Heatr9	A	T	11: 83,518,792 (GRCm38)	I118N	possibly damaging	Het
Htra3	T	C	5: 35,671,125 (GRCm38)	H137R	probably benign	Het
Iars	A	G	13: 49,709,661 (GRCm38)	S530G	probably damaging	Het
Klhdc4	T	A	8: 121,796,603 (GRCm38)	M510L	probably benign	Het
Lipc	T	C	9: 70,816,731 (GRCm38)	T204A	probably benign	Het
Lrp1	A	G	10: 127,553,779 (GRCm38)	V3129A	probably damaging	Het
Macf1	G	A	4: 123,494,909 (GRCm38)	T1120I	probably benign	Het
Map4	T	C	9: 110,038,377 (GRCm38)		probably benign	Het
Mbtps1	A	T	8: 119,533,348 (GRCm38)	V420D	probably damaging	Het
Mta2	G	T	19: 8,950,383 (GRCm38)	D523Y	probably benign	Het
Muc4	A	G	16: 32,756,296 (GRCm38)		probably benign	Het
Mug1	C	T	6: 121,878,943 (GRCm38)	Q965*	probably null	Het
Myo1e	T	A	9: 70,353,312 (GRCm38)	I584N	probably damaging	Het
Nolc1	T	C	19: 46,078,920 (GRCm38)	V80A	probably damaging	Het
Nop56	G	T	2: 130,275,725 (GRCm38)	V91L	probably benign	Het
Olfr1451	A	T	19: 12,999,219 (GRCm38)	M78L	probably benign	Het
Olfr191	A	T	16: 59,086,402 (GRCm38)	L27Q	probably damaging	Het
Olfr523	T	C	7: 140,177,020 (GRCm38)	V300A	probably damaging	Het
Osbpl3	T	C	6: 50,336,297 (GRCm38)	E107G	probably damaging	Het
Pde3a	T	A	6: 141,250,025 (GRCm38)	C146S	probably benign	Het
Pfkm	A	G	15: 98,128,242 (GRCm38)	M573V	probably damaging	Het
Pkd1l2	C	T	8: 117,047,374 (GRCm38)		probably null	Het
Plekhg3	T	C	12: 76,565,247 (GRCm38)	I374T	possibly damaging	Het
Ppig	T	A	2: 69,741,486 (GRCm38)	V183D	unknown	Het
Prom1	T	G	5: 44,037,534 (GRCm38)	I290L	probably benign	Het
Rufy3	T	A	5: 88,637,226 (GRCm38)	M387K	probably damaging	Het
Selenoo	G	A	15: 89,094,184 (GRCm38)	R270H	probably damaging	Het
Sema3d	T	A	5: 12,508,087 (GRCm38)		probably null	Het
Sema6c	C	T	3: 95,168,363 (GRCm38)	T175I	probably damaging	Het
Serpina3k	T	C	12: 104,341,046 (GRCm38)	I179T	probably damaging	Het
Sf3b3	T	C	8: 110,841,203 (GRCm38)	T207A	probably benign	Het
Slc22a26	C	A	19: 7,802,181 (GRCm38)	R90L	probably damaging	Het
Slitrk5	T	C	14: 111,680,216 (GRCm38)	V424A	probably damaging	Het
Smarca2	G	T	19: 26,720,855 (GRCm38)	E89*	probably null	Het
Soga1	C	T	2: 157,022,856 (GRCm38)	G1144D	probably benign	Het
Stab2	T	A	10: 86,937,909 (GRCm38)	S853C	probably damaging	Het
Stk17b	A	T	1: 53,761,147 (GRCm38)		probably null	Het
Taar5	T	A	10: 23,971,547 (GRCm38)	I281N	possibly damaging	Het
Tarsl2	A	T	7: 65,658,935 (GRCm38)	E284D	probably damaging	Het
Tbx15	C	A	3: 99,316,333 (GRCm38)	T279K	probably damaging	Het
Tfr2	G	A	5: 137,586,925 (GRCm38)	V740I	probably benign	Het
Tlr12	T	C	4: 128,617,680 (GRCm38)	E259G	probably benign	Het
Tmem145	A	G	7: 25,309,034 (GRCm38)	T302A	probably benign	Het
Tmem57	A	G	4: 134,828,133 (GRCm38)	I343T	probably benign	Het
Trpa1	G	T	1: 14,875,861 (GRCm38)	H1015Q	probably benign	Het
Tspan8	A	G	10: 115,817,629 (GRCm38)	Y10C	possibly damaging	Het
Ttll7	A	G	3: 146,894,469 (GRCm38)	N44S	probably damaging	Het
Uba7	T	C	9: 107,979,839 (GRCm38)		probably null	Het
Ube2j2	G	A	4: 155,946,384 (GRCm38)	M1I	probably null	Het
Ubr5	C	A	15: 38,009,668 (GRCm38)	A1022S	probably benign	Het
Usf1	T	G	1: 171,415,763 (GRCm38)	I36S	probably damaging	Het
Vmn1r181	A	T	7: 23,984,365 (GRCm38)	D85V	probably damaging	Het
Vmn1r3	A	T	4: 3,185,009 (GRCm38)	Y99*	probably null	Het
Vmn1r72	T	C	7: 11,670,373 (GRCm38)	I49M	possibly damaging	Het
Vsig10	T	A	5: 117,343,975 (GRCm38)	V410E	probably damaging	Het
Zc3h7b	A	G	15: 81,779,133 (GRCm38)	Y442C	probably damaging	Het
Zfyve26	G	T	12: 79,280,385 (GRCm38)	Y730*	probably null	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,378,854 (GRCm38)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,394,256 (GRCm38)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,382,623 (GRCm38)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,393,658 (GRCm38)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,380,115 (GRCm38)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,380,232 (GRCm38)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,378,690 (GRCm38)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,378,223 (GRCm38)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,394,343 (GRCm38)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,394,573 (GRCm38)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,379,043 (GRCm38)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,362,259 (GRCm38)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,380,060 (GRCm38)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,362,252 (GRCm38)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,377,945 (GRCm38)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,362,153 (GRCm38)	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19,378,815 (GRCm38)	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19,377,991 (GRCm38)	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19,378,629 (GRCm38)	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19,378,708 (GRCm38)	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19,394,373 (GRCm38)	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,378,570 (GRCm38)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,379,260 (GRCm38)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,393,662 (GRCm38)	missense	probably damaging	1.00
R5206:Vmn2r99	UTSW	17	19,378,606 (GRCm38)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,379,339 (GRCm38)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,379,269 (GRCm38)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,394,146 (GRCm38)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,377,948 (GRCm38)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,378,980 (GRCm38)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,382,605 (GRCm38)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,380,031 (GRCm38)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,380,034 (GRCm38)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,380,195 (GRCm38)	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19,378,110 (GRCm38)	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19,394,564 (GRCm38)	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19,393,710 (GRCm38)	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19,379,311 (GRCm38)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,379,145 (GRCm38)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,393,817 (GRCm38)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,380,040 (GRCm38)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,393,758 (GRCm38)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,394,181 (GRCm38)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,393,660 (GRCm38)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,378,126 (GRCm38)	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19,378,627 (GRCm38)	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19,362,301 (GRCm38)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,379,301 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- TCAGCAGCAGAGTCCATGTG -3'
(R):5'- CACAGTTGAAGTTTCCATTCCC -3'

Sequencing Primer
(F):5'- AGTGTGACAGACTGAGTC -3'
(R):5'- TCCCCCTGAGTGAAGAAAATAATCTG -3'

Posted On

2016-06-06