Mutagenetix > Incidental Mutation

Incidental Mutation 'R5005:Vmn2r71'

390140

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r71

Ensembl Gene

ENSMUSG00000091205

Gene Name

vomeronasal 2, receptor 71

Synonyms

EG233445

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R5005 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85264670-85273755 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 85273352 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 722 (V722D)

Ref Sequence

ENSEMBL: ENSMUSP00000132337 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172338]

AlphaFold

L7N2D8

Predicted Effect

probably damaging
Transcript: ENSMUST00000172338
AA Change: V722D

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000132337
Gene: ENSMUSG00000091205
AA Change: V722D

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	468	2.1e-31	PFAM
Pfam:NCD3G	511	563	8.7e-20	PFAM
Pfam:7tm_3	593	831	2e-55	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000208273

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ap2b1	T	C	11: 83,230,218 (GRCm39)	V412A	probably damaging	Het
Asic2	T	C	11: 80,774,252 (GRCm39)	Y455C	probably damaging	Het
Bbx	T	C	16: 50,086,714 (GRCm39)	K61E	probably damaging	Het
Cd207	T	C	6: 83,651,367 (GRCm39)	E196G	possibly damaging	Het
Chn1	T	A	2: 73,490,130 (GRCm39)	Q49L	possibly damaging	Het
Cpd	A	G	11: 76,704,396 (GRCm39)	I406T	probably damaging	Het
D630003M21Rik	C	T	2: 158,053,563 (GRCm39)	V642I	possibly damaging	Het
Dnah7b	C	T	1: 46,281,188 (GRCm39)	L2750F	probably damaging	Het
Epyc	A	G	10: 97,510,562 (GRCm39)	T122A	probably benign	Het
Kcnt1	A	G	2: 25,791,358 (GRCm39)	H567R	probably damaging	Het
Magi2	A	G	5: 20,739,444 (GRCm39)	D729G	probably damaging	Het
Myh4	G	T	11: 67,144,241 (GRCm39)	V1204L	probably benign	Het
Noa1	T	C	5: 77,456,873 (GRCm39)	Y344C	probably damaging	Het
Or1j19	A	G	2: 36,677,370 (GRCm39)	M278V	probably benign	Het
Or4c102	A	G	2: 88,422,348 (GRCm39)	M67V	probably benign	Het
Pex1	T	C	5: 3,672,310 (GRCm39)	S718P	probably damaging	Het
Plxnb1	T	A	9: 108,935,647 (GRCm39)	V1061E	probably benign	Het
Rdh16f1	A	G	10: 127,624,546 (GRCm39)	Q128R	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Slc26a9	A	T	1: 131,693,625 (GRCm39)	Q705L	probably damaging	Het
Tas2r143	T	A	6: 42,377,658 (GRCm39)	C163S	probably benign	Het
Tigd2	A	G	6: 59,188,131 (GRCm39)	T333A	probably benign	Het
Urb2	T	C	8: 124,757,920 (GRCm39)	V1209A	probably damaging	Het

Other mutations in Vmn2r71

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00922:Vmn2r71	APN	7	85,267,901 (GRCm39)	missense	probably benign
IGL00960:Vmn2r71	APN	7	85,273,582 (GRCm39)	missense	probably damaging	1.00
IGL01372:Vmn2r71	APN	7	85,270,022 (GRCm39)	splice site	probably benign
IGL01690:Vmn2r71	APN	7	85,264,782 (GRCm39)	missense	probably damaging	1.00
IGL01909:Vmn2r71	APN	7	85,270,001 (GRCm39)	missense	probably benign	0.00
IGL01950:Vmn2r71	APN	7	85,264,827 (GRCm39)	missense	probably damaging	0.98
IGL02570:Vmn2r71	APN	7	85,264,748 (GRCm39)	missense	possibly damaging	0.95
IGL02650:Vmn2r71	APN	7	85,273,535 (GRCm39)	missense	probably damaging	1.00
IGL02901:Vmn2r71	APN	7	85,268,470 (GRCm39)	missense	probably benign	0.00
IGL03128:Vmn2r71	APN	7	85,268,795 (GRCm39)	missense	probably damaging	1.00
IGL03328:Vmn2r71	APN	7	85,273,499 (GRCm39)	missense	probably damaging	1.00
R0533:Vmn2r71	UTSW	7	85,268,426 (GRCm39)	frame shift	probably null
R0707:Vmn2r71	UTSW	7	85,268,640 (GRCm39)	missense	probably benign
R0841:Vmn2r71	UTSW	7	85,267,749 (GRCm39)	missense	possibly damaging	0.62
R0865:Vmn2r71	UTSW	7	85,268,516 (GRCm39)	missense	probably benign	0.01
R0883:Vmn2r71	UTSW	7	85,272,842 (GRCm39)	missense	probably benign	0.19
R0939:Vmn2r71	UTSW	7	85,272,889 (GRCm39)	missense	possibly damaging	0.70
R1597:Vmn2r71	UTSW	7	85,273,352 (GRCm39)	missense	possibly damaging	0.46
R1646:Vmn2r71	UTSW	7	85,270,476 (GRCm39)	missense	probably damaging	0.99
R1719:Vmn2r71	UTSW	7	85,270,435 (GRCm39)	missense	probably damaging	1.00
R1860:Vmn2r71	UTSW	7	85,264,782 (GRCm39)	missense	probably damaging	1.00
R2013:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2014:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2015:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2050:Vmn2r71	UTSW	7	85,273,681 (GRCm39)	missense	probably damaging	1.00
R2084:Vmn2r71	UTSW	7	85,267,945 (GRCm39)	missense	probably benign	0.03
R2221:Vmn2r71	UTSW	7	85,273,301 (GRCm39)	missense	probably benign	0.40
R2223:Vmn2r71	UTSW	7	85,273,301 (GRCm39)	missense	probably benign	0.40
R2245:Vmn2r71	UTSW	7	85,273,388 (GRCm39)	missense	probably damaging	1.00
R3115:Vmn2r71	UTSW	7	85,272,866 (GRCm39)	missense	probably damaging	0.97
R3122:Vmn2r71	UTSW	7	85,264,828 (GRCm39)	nonsense	probably null
R3609:Vmn2r71	UTSW	7	85,268,870 (GRCm39)	missense	probably damaging	1.00
R4093:Vmn2r71	UTSW	7	85,270,442 (GRCm39)	missense	probably benign	0.00
R4305:Vmn2r71	UTSW	7	85,273,360 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r71	UTSW	7	85,273,360 (GRCm39)	missense	probably damaging	1.00
R4334:Vmn2r71	UTSW	7	85,269,042 (GRCm39)	missense	probably benign	0.01
R4569:Vmn2r71	UTSW	7	85,273,402 (GRCm39)	missense	possibly damaging	0.66
R4622:Vmn2r71	UTSW	7	85,269,817 (GRCm39)	missense	probably benign	0.00
R4915:Vmn2r71	UTSW	7	85,270,476 (GRCm39)	missense	probably damaging	0.99
R4956:Vmn2r71	UTSW	7	85,268,436 (GRCm39)	missense	probably benign	0.19
R5045:Vmn2r71	UTSW	7	85,273,597 (GRCm39)	missense	probably benign	0.00
R5153:Vmn2r71	UTSW	7	85,268,430 (GRCm39)	missense	possibly damaging	0.94
R5236:Vmn2r71	UTSW	7	85,272,877 (GRCm39)	missense	probably damaging	1.00
R5373:Vmn2r71	UTSW	7	85,267,750 (GRCm39)	missense	possibly damaging	0.79
R5405:Vmn2r71	UTSW	7	85,268,622 (GRCm39)	missense	probably benign
R5831:Vmn2r71	UTSW	7	85,272,922 (GRCm39)	missense	probably benign	0.16
R6061:Vmn2r71	UTSW	7	85,268,482 (GRCm39)	missense	probably benign
R6518:Vmn2r71	UTSW	7	85,270,436 (GRCm39)	missense	probably damaging	1.00
R6751:Vmn2r71	UTSW	7	85,269,095 (GRCm39)	critical splice donor site	probably null
R6920:Vmn2r71	UTSW	7	85,273,108 (GRCm39)	missense	probably damaging	1.00
R7358:Vmn2r71	UTSW	7	85,273,468 (GRCm39)	missense	possibly damaging	0.81
R7453:Vmn2r71	UTSW	7	85,273,297 (GRCm39)	missense	probably benign	0.21
R7560:Vmn2r71	UTSW	7	85,273,115 (GRCm39)	missense	probably benign	0.06
R7871:Vmn2r71	UTSW	7	85,272,869 (GRCm39)	missense	possibly damaging	0.81
R8267:Vmn2r71	UTSW	7	85,264,704 (GRCm39)	missense	probably benign	0.02
R8377:Vmn2r71	UTSW	7	85,264,707 (GRCm39)	missense	probably benign
R9278:Vmn2r71	UTSW	7	85,269,788 (GRCm39)	missense	probably benign	0.19
R9319:Vmn2r71	UTSW	7	85,273,694 (GRCm39)	missense	probably damaging	1.00
R9329:Vmn2r71	UTSW	7	85,267,950 (GRCm39)	missense	probably benign	0.00
R9368:Vmn2r71	UTSW	7	85,273,442 (GRCm39)	missense	probably damaging	1.00
R9636:Vmn2r71	UTSW	7	85,268,388 (GRCm39)	missense	possibly damaging	0.80
R9756:Vmn2r71	UTSW	7	85,268,573 (GRCm39)	nonsense	probably null
X0025:Vmn2r71	UTSW	7	85,267,873 (GRCm39)	missense	probably benign
Z1186:Vmn2r71	UTSW	7	85,273,094 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAGATCACATTTGCCATTGG -3'
(R):5'- ACACCAGCATGCTGAATGTC -3'

Sequencing Primer
(F):5'- AGATCACATTTGCCATTGGGTTCAC -3'
(R):5'- CATGCTGAATGTCAAGAGCTTGGC -3'

Posted On

2016-06-06