Mutagenetix > Incidental Mutation

Incidental Mutation 'R5006:Ciao2a'

390169

Institutional Source

Beutler Lab

Gene Symbol

Ciao2a

Ensembl Gene

ENSMUSG00000032381

Gene Name

cytosolic iron-sulfur assembly component 2A

Synonyms

Fam96a, 5730536A07Rik

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5006 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66033893-66046237 bp(+) (GRCm39)

Type of Mutation

splice site (5 bp from exon)

DNA Base Change (assembly)

G to A at 66043634 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000034945 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034945]

AlphaFold

Q9DCL2

Predicted Effect

probably null
Transcript: ENSMUST00000034945

SMART Domains

Protein: ENSMUSP00000034945
Gene: ENSMUSG00000032381

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
Pfam:DUF59	38	115	4.4e-12	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.7%
20x: 93.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 21 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf2	C	A	5: 24,781,535 (GRCm39)	E51*	probably null	Het
Bcl11a	T	A	11: 24,114,989 (GRCm39)	Y777*	probably null	Het
C2cd4c	T	A	10: 79,448,341 (GRCm39)	T269S	probably benign	Het
Efcab3	T	A	11: 104,620,503 (GRCm39)		probably null	Het
Foxp1	A	C	6: 99,139,819 (GRCm39)	V54G	probably damaging	Het
Il2ra	T	G	2: 11,679,157 (GRCm39)	L39R	possibly damaging	Het
Klk1b9	A	T	7: 43,628,711 (GRCm39)	K72*	probably null	Het
Lmo7	A	G	14: 102,163,673 (GRCm39)		probably benign	Het
Mfng	C	T	15: 78,648,588 (GRCm39)	R163H	probably benign	Het
Nebl	T	A	2: 17,393,582 (GRCm39)		probably null	Het
Or6b6	C	T	7: 106,570,808 (GRCm39)	V248I	probably damaging	Het
Or7e169	G	T	9: 19,757,567 (GRCm39)	A116E	probably benign	Het
Or7g30	A	G	9: 19,352,545 (GRCm39)	N112S	probably benign	Het
Ralgapa1	A	T	12: 55,764,899 (GRCm39)	C918S	probably benign	Het
Rasd2	G	T	8: 75,945,234 (GRCm39)	R21L	probably damaging	Het
Sema4a	A	G	3: 88,344,091 (GRCm39)	M720T	probably benign	Het
Susd3	A	G	13: 49,392,181 (GRCm39)		probably benign	Het
Vmn1r235	T	C	17: 21,482,467 (GRCm39)	M264T	probably benign	Het
Wls	A	G	3: 159,617,428 (GRCm39)	I368V	possibly damaging	Het
Ypel4	A	G	2: 84,567,182 (GRCm39)	D5G	probably benign	Het
Zbtb38	C	T	9: 96,567,704 (GRCm39)	G1127S	probably damaging	Het

Other mutations in Ciao2a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00565:Ciao2a	APN	9	66,039,898 (GRCm39)	missense	probably benign	0.00
R1905:Ciao2a	UTSW	9	66,039,929 (GRCm39)	missense	probably benign
R6471:Ciao2a	UTSW	9	66,034,139 (GRCm39)	missense	possibly damaging	0.91
R7947:Ciao2a	UTSW	9	66,045,684 (GRCm39)	missense	probably benign	0.01
R8773:Ciao2a	UTSW	9	66,045,667 (GRCm39)	missense	probably damaging	1.00
R8858:Ciao2a	UTSW	9	66,039,824 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TACAGAAATCCAAGGGCACG -3'
(R):5'- TATGTAGCCTGCCATCAGAACC -3'

Sequencing Primer
(F):5'- CAGGTCAGTTGGTGGAAATGC -3'
(R):5'- TAGCCTGCCATCAGAACCAGTTTAC -3'

Posted On

2016-06-06