Mutagenetix > Incidental Mutation

Incidental Mutation 'R5057:Lama3'

390989

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

042647-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5057 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

12466876-12716070 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 12665005 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 2275 (G2275S)

Ref Sequence

ENSEMBL: ENSMUSP00000089703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070] [ENSMUST00000188815]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000092070
AA Change: G2275S

PolyPhen 2 Score 0.819 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: G2275S

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000188815
AA Change: G669S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000140104
Gene: ENSMUSG00000024421
AA Change: G669S

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
EGF_Lam	78	122	2.66e-10	SMART
EGF_Lam	125	175	7.81e-8	SMART
Pfam:Laminin_I	230	496	1e-90	PFAM
low complexity region	579	594	N/A	INTRINSIC
coiled coil region	605	632	N/A	INTRINSIC
LamG	800	960	1.67e-2	SMART
LamG	1008	1136	1.72e-17	SMART
LamG	1179	1294	3.96e-17	SMART
LamG	1399	1527	1.12e-34	SMART
LamG	1569	1702	3.41e-30	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.0%

Validation Efficiency

97% (118/122)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 120 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	A	T	11: 72,057,581 (GRCm39)	H739Q	probably benign	Het
Acad12	T	A	5: 121,748,152 (GRCm39)	T89S	probably benign	Het
Adgb	A	G	10: 10,233,722 (GRCm39)	C1252R	probably benign	Het
Ankef1	A	G	2: 136,392,280 (GRCm39)		probably null	Het
Ankfy1	T	C	11: 72,650,745 (GRCm39)	L976P	probably damaging	Het
Ankib1	T	A	5: 3,784,011 (GRCm39)	I322F	possibly damaging	Het
Ankrd61	T	A	5: 143,831,613 (GRCm39)	T64S	probably benign	Het
Anxa6	T	G	11: 54,892,062 (GRCm39)	E298A	possibly damaging	Het
Apc2	A	G	10: 80,144,903 (GRCm39)	S605G	probably damaging	Het
Arih1	T	C	9: 59,393,515 (GRCm39)	N39S	unknown	Het
Bicc1	A	G	10: 70,783,713 (GRCm39)	S393P	possibly damaging	Het
Bptf	A	T	11: 106,973,354 (GRCm39)	F693L	probably damaging	Het
Cacng4	A	G	11: 107,685,197 (GRCm39)	Y32H	probably damaging	Het
Ccdc50	T	A	16: 27,257,092 (GRCm39)	D252E	probably benign	Het
Ccdc92b	C	A	11: 74,528,976 (GRCm39)	T160K	probably damaging	Het
Cd163	C	T	6: 124,302,247 (GRCm39)	T937I	probably damaging	Het
Cdh22	C	A	2: 164,958,063 (GRCm39)	V635F	probably damaging	Het
Cenpe	G	T	3: 134,926,074 (GRCm39)	A243S	probably benign	Het
Cep295	A	T	9: 15,233,979 (GRCm39)	H2272Q	probably benign	Het
Cgnl1	A	T	9: 71,632,076 (GRCm39)	V425D	probably damaging	Het
Clec4b2	A	T	6: 123,177,915 (GRCm39)	S77C	probably null	Het
Cntnap5a	C	T	1: 115,612,943 (GRCm39)	T26I	probably benign	Het
Col6a3	T	C	1: 90,743,852 (GRCm39)	K165R	possibly damaging	Het
Copb1	A	T	7: 113,825,997 (GRCm39)	N662K	probably benign	Het
Crybg1	T	A	10: 43,865,104 (GRCm39)	R1458*	probably null	Het
Cspp1	T	A	1: 10,145,186 (GRCm39)		probably benign	Het
Dhtkd1	A	G	2: 5,924,324 (GRCm39)	C430R	probably damaging	Het
Dlg5	T	C	14: 24,186,690 (GRCm39)	E1847G	probably damaging	Het
Dync2i1	A	T	12: 116,177,033 (GRCm39)	N856K	probably benign	Het
Eif1ad16	A	T	12: 87,985,481 (GRCm39)	S21T	unknown	Het
Ephb3	A	G	16: 21,039,197 (GRCm39)	D351G	probably damaging	Het
Exd2	T	A	12: 80,543,564 (GRCm39)	N582K	probably damaging	Het
Fam161a	T	A	11: 22,970,397 (GRCm39)	C192S	probably damaging	Het
Fbn1	A	G	2: 125,308,615 (GRCm39)	V149A	probably benign	Het
Fbxw16	A	G	9: 109,270,232 (GRCm39)	S170P	probably damaging	Het
Fndc1	T	A	17: 7,990,802 (GRCm39)	R965*	probably null	Het
Frem2	T	C	3: 53,442,617 (GRCm39)	D2640G	probably benign	Het
Gfm1	T	C	3: 67,380,877 (GRCm39)	V664A	probably damaging	Het
Gm10803	A	C	2: 93,394,517 (GRCm39)	L96F	probably damaging	Het
Gm14569	T	C	X: 35,694,470 (GRCm39)	D1413G	probably benign	Het
Gm44501	A	G	17: 40,889,563 (GRCm39)	T26A	probably benign	Het
Gpc4	G	A	X: 51,163,440 (GRCm39)	R148C	probably damaging	Het
Gpsm1	G	A	2: 26,215,369 (GRCm39)	R277Q	probably damaging	Het
Inmt	T	A	6: 55,151,883 (GRCm39)	H29L	probably benign	Het
Insrr	T	A	3: 87,722,572 (GRCm39)	C1265S	probably benign	Het
Kctd12	A	G	14: 103,219,045 (GRCm39)	F278L	possibly damaging	Het
Klk14	G	A	7: 43,341,501 (GRCm39)	C51Y	probably damaging	Het
Lag3	T	A	6: 124,882,318 (GRCm39)	I393F	possibly damaging	Het
Lama2	C	T	10: 27,040,982 (GRCm39)	C1447Y	probably damaging	Het
Lrrc17	A	T	5: 21,780,307 (GRCm39)	H427L	probably benign	Het
Med13l	T	A	5: 118,856,558 (GRCm39)	S164T	probably damaging	Het
Milr1	A	G	11: 106,657,791 (GRCm39)	D131G	possibly damaging	Het
Myo5c	A	T	9: 75,208,155 (GRCm39)	T1630S	probably damaging	Het
Nbeal2	A	T	9: 110,460,073 (GRCm39)	N1848K	probably damaging	Het
Ncor2	A	T	5: 125,125,130 (GRCm39)	V307E	possibly damaging	Het
Ndufs3	C	A	2: 90,729,004 (GRCm39)	A161S	probably benign	Het
Neo1	T	C	9: 58,897,554 (GRCm39)	D134G	probably damaging	Het
Nexmif	A	T	X: 103,130,956 (GRCm39)	N320K	probably damaging	Het
Nipal3	T	A	4: 135,194,167 (GRCm39)	I289F	probably damaging	Het
Nrg4	A	G	9: 55,189,880 (GRCm39)		probably benign	Het
Nrxn3	A	T	12: 89,221,804 (GRCm39)	I528F	probably damaging	Het
Or11h23	A	C	14: 50,948,669 (GRCm39)	N294T	probably damaging	Het
Or3a10	T	A	11: 73,935,881 (GRCm39)	D73V	probably damaging	Het
Pate2	T	C	9: 35,597,407 (GRCm39)		probably benign	Het
Pcnx2	T	C	8: 126,581,930 (GRCm39)	I935M	possibly damaging	Het
Pik3c2a	G	T	7: 115,975,518 (GRCm39)	T683K	possibly damaging	Het
Pip5k1c	G	A	10: 81,146,723 (GRCm39)		probably null	Het
Pkd1l2	G	A	8: 117,781,747 (GRCm39)	T766I	probably benign	Het
Polr3c	C	A	3: 96,619,373 (GRCm39)	L508F	probably damaging	Het
Por	A	G	5: 135,759,756 (GRCm39)	D189G	probably damaging	Het
Potefam1	A	T	2: 111,055,766 (GRCm39)	F207I	probably benign	Het
Prim2	A	T	1: 33,669,441 (GRCm39)	L178*	probably null	Het
Prima1	C	A	12: 103,168,864 (GRCm39)		probably null	Het
Ptpn21	G	A	12: 98,645,666 (GRCm39)	R1091C	probably damaging	Het
Ptpn23	G	A	9: 110,217,624 (GRCm39)	T744I	probably benign	Het
Rad21	A	T	15: 51,830,102 (GRCm39)	I503K	probably benign	Het
Rbm12	A	T	2: 155,938,806 (GRCm39)	C489S	probably benign	Het
Rpl10a	A	T	17: 28,549,607 (GRCm39)	H140L	probably benign	Het
Rpl7-ps8	A	T	15: 59,083,004 (GRCm39)		noncoding transcript	Het
Rtbdn	T	C	8: 85,681,638 (GRCm39)	F143S	probably damaging	Het
Scamp3	T	A	3: 89,089,600 (GRCm39)		probably benign	Het
Setd5	A	G	6: 113,114,922 (GRCm39)	S848G	probably damaging	Het
Sez6	T	C	11: 77,863,979 (GRCm39)	V487A	probably damaging	Het
Shc2	G	A	10: 79,459,706 (GRCm39)	P413S	probably benign	Het
Sipa1l3	T	C	7: 29,070,618 (GRCm39)	N966S	probably damaging	Het
Slc39a8	A	G	3: 135,554,790 (GRCm39)	E78G	probably benign	Het
Slc8a3	A	G	12: 81,246,332 (GRCm39)	V900A	probably damaging	Het
Slitrk3	T	C	3: 72,957,981 (GRCm39)	T264A	probably benign	Het
Spire2	G	A	8: 124,084,940 (GRCm39)	R260H	probably damaging	Het
Stk39	T	A	2: 68,051,292 (GRCm39)	I518F	probably damaging	Het
Tardbp	T	A	4: 148,703,813 (GRCm39)		probably null	Het
Tep1	A	G	14: 51,066,456 (GRCm39)	Y2335H	probably benign	Het
Tesl1	G	A	X: 23,773,480 (GRCm39)	G327E	probably damaging	Het
Tll2	A	G	19: 41,105,705 (GRCm39)	V358A	probably benign	Het
Tmem268	C	G	4: 63,486,777 (GRCm39)	S100C	probably damaging	Het
Tnfsf9	A	G	17: 57,412,444 (GRCm39)	T5A	probably benign	Het
Trappc10	G	T	10: 78,052,994 (GRCm39)	F260L	possibly damaging	Het
Trim5	A	G	7: 103,914,630 (GRCm39)	Y480H	probably damaging	Het
Trmt112	T	C	19: 6,888,121 (GRCm39)	V91A	probably benign	Het
Ttn	T	A	2: 76,577,379 (GRCm39)	K22759*	probably null	Het
Ttn	C	A	2: 76,748,988 (GRCm39)	L4020F	probably benign	Het
Ubash3b	A	G	9: 40,948,755 (GRCm39)	C187R	possibly damaging	Het
Ube3b	T	C	5: 114,544,318 (GRCm39)	F572L	probably benign	Het
Ubr5	A	G	15: 38,004,353 (GRCm39)	V1332A	probably damaging	Het
Ubtd2	C	A	11: 32,466,320 (GRCm39)	R180S	probably damaging	Het
Ubtf	A	G	11: 102,197,913 (GRCm39)	S673P	probably damaging	Het
Ubtfl1	A	T	9: 18,320,487 (GRCm39)	D5V	possibly damaging	Het
Usp17la	G	A	7: 104,510,330 (GRCm39)	V312I	possibly damaging	Het
Usp17ld	G	T	7: 102,899,655 (GRCm39)	H426N	probably benign	Het
Usp34	A	G	11: 23,408,086 (GRCm39)		probably benign	Het
Vmn1r199	A	T	13: 22,567,575 (GRCm39)	T290S	possibly damaging	Het
Vmn1r33	T	C	6: 66,589,089 (GRCm39)	N155S	probably benign	Het
Vmn2r28	T	A	7: 5,489,463 (GRCm39)	I459L	probably benign	Het
Vmn2r6	T	A	3: 64,445,207 (GRCm39)	K839N	probably damaging	Het
Vmn2r65	A	G	7: 84,589,819 (GRCm39)	I699T	probably damaging	Het
Vps16	A	G	2: 130,281,372 (GRCm39)	S235G	probably benign	Het
Vwde	T	C	6: 13,192,641 (GRCm39)	I421V	possibly damaging	Het
Xiap	T	C	X: 41,183,342 (GRCm39)	F23L	probably benign	Het
Xkr7	A	G	2: 152,896,300 (GRCm39)	T385A	probably benign	Het
Znfx1	T	C	2: 166,881,746 (GRCm39)	Y217C	probably damaging	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,713,349 (GRCm39)	missense	probably benign
IGL00272:Lama3	APN	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,582,645 (GRCm39)	splice site	probably benign
IGL00836:Lama3	APN	18	12,605,285 (GRCm39)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,574,200 (GRCm39)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,614,094 (GRCm39)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,664,983 (GRCm39)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,574,188 (GRCm39)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,586,937 (GRCm39)	splice site	probably benign
IGL01863:Lama3	APN	18	12,552,993 (GRCm39)	splice site	probably benign
IGL01869:Lama3	APN	18	12,657,820 (GRCm39)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,705,121 (GRCm39)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,649,570 (GRCm39)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,601,371 (GRCm39)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,714,840 (GRCm39)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,624,533 (GRCm39)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,689,807 (GRCm39)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,690,784 (GRCm39)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,670,915 (GRCm39)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,658,910 (GRCm39)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,711,184 (GRCm39)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,661,858 (GRCm39)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,601,425 (GRCm39)	splice site	probably benign
IGL03038:Lama3	APN	18	12,552,307 (GRCm39)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,572,406 (GRCm39)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,660,681 (GRCm39)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,614,095 (GRCm39)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,672,760 (GRCm39)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,686,340 (GRCm39)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,552,239 (GRCm39)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,686,288 (GRCm39)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,673,024 (GRCm39)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0007:Lama3	UTSW	18	12,630,938 (GRCm39)	splice site	probably benign
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,537,160 (GRCm39)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0063:Lama3	UTSW	18	12,661,762 (GRCm39)	splice site	probably benign
R0106:Lama3	UTSW	18	12,537,039 (GRCm39)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,581,329 (GRCm39)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,657,867 (GRCm39)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0240:Lama3	UTSW	18	12,672,880 (GRCm39)	splice site	probably null
R0316:Lama3	UTSW	18	12,652,934 (GRCm39)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,615,183 (GRCm39)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,640,064 (GRCm39)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,540,620 (GRCm39)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,589,894 (GRCm39)	missense	probably benign
R0449:Lama3	UTSW	18	12,633,569 (GRCm39)	splice site	probably null
R0453:Lama3	UTSW	18	12,598,535 (GRCm39)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,583,481 (GRCm39)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,658,951 (GRCm39)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,694,758 (GRCm39)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,682,309 (GRCm39)	missense	probably benign
R0605:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,552,315 (GRCm39)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,552,302 (GRCm39)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,610,647 (GRCm39)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,589,907 (GRCm39)	splice site	probably benign
R1216:Lama3	UTSW	18	12,554,191 (GRCm39)	splice site	probably benign
R1356:Lama3	UTSW	18	12,633,634 (GRCm39)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,610,427 (GRCm39)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,653,048 (GRCm39)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,614,155 (GRCm39)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,682,284 (GRCm39)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,574,164 (GRCm39)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,615,102 (GRCm39)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,646,788 (GRCm39)	splice site	probably benign
R1571:Lama3	UTSW	18	12,672,774 (GRCm39)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,583,457 (GRCm39)	nonsense	probably null
R1631:Lama3	UTSW	18	12,540,551 (GRCm39)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,665,256 (GRCm39)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,612,929 (GRCm39)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,598,556 (GRCm39)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,535,119 (GRCm39)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,646,762 (GRCm39)	missense	possibly damaging	0.75
R1856:Lama3	UTSW	18	12,670,838 (GRCm39)	nonsense	probably null
R1909:Lama3	UTSW	18	12,714,855 (GRCm39)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,628,336 (GRCm39)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,586,920 (GRCm39)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,657,778 (GRCm39)	splice site	probably benign
R2059:Lama3	UTSW	18	12,661,390 (GRCm39)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,661,783 (GRCm39)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,657,887 (GRCm39)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,535,906 (GRCm39)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,658,136 (GRCm39)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,586,807 (GRCm39)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,546,915 (GRCm39)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,581,345 (GRCm39)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,572,532 (GRCm39)	splice site	probably benign
R3752:Lama3	UTSW	18	12,640,086 (GRCm39)	missense	probably damaging	1.00
R3967:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,713,398 (GRCm39)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,637,365 (GRCm39)	nonsense	probably null
R4118:Lama3	UTSW	18	12,583,488 (GRCm39)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,646,747 (GRCm39)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,715,588 (GRCm39)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,652,929 (GRCm39)	nonsense	probably null
R4483:Lama3	UTSW	18	12,682,310 (GRCm39)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,614,145 (GRCm39)	missense	probably benign
R4516:Lama3	UTSW	18	12,628,415 (GRCm39)	missense	probably damaging	1.00
R4556:Lama3	UTSW	18	12,612,816 (GRCm39)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,637,454 (GRCm39)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,711,086 (GRCm39)	nonsense	probably null
R4704:Lama3	UTSW	18	12,686,280 (GRCm39)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,637,416 (GRCm39)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,615,141 (GRCm39)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,633,620 (GRCm39)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,546,828 (GRCm39)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,544,627 (GRCm39)	nonsense	probably null
R4784:Lama3	UTSW	18	12,582,601 (GRCm39)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,610,661 (GRCm39)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,574,188 (GRCm39)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,544,599 (GRCm39)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,631,735 (GRCm39)	intron	probably benign
R4863:Lama3	UTSW	18	12,672,850 (GRCm39)	missense	probably damaging	0.99
R4953:Lama3	UTSW	18	12,581,362 (GRCm39)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,685,883 (GRCm39)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,651,800 (GRCm39)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,715,668 (GRCm39)	missense	probably benign	0.19
R5090:Lama3	UTSW	18	12,675,459 (GRCm39)	missense	possibly damaging	0.94
R5122:Lama3	UTSW	18	12,672,823 (GRCm39)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,710,957 (GRCm39)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,552,950 (GRCm39)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,598,565 (GRCm39)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,685,912 (GRCm39)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,586,803 (GRCm39)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,705,123 (GRCm39)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,589,821 (GRCm39)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,686,267 (GRCm39)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,605,277 (GRCm39)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,572,405 (GRCm39)	missense	probably benign
R5617:Lama3	UTSW	18	12,631,993 (GRCm39)	intron	probably benign
R5709:Lama3	UTSW	18	12,672,856 (GRCm39)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,562,944 (GRCm39)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,707,311 (GRCm39)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,602,985 (GRCm39)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,615,156 (GRCm39)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,646,702 (GRCm39)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,657,794 (GRCm39)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,640,006 (GRCm39)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,615,194 (GRCm39)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,612,813 (GRCm39)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,615,205 (GRCm39)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,628,405 (GRCm39)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,670,823 (GRCm39)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,710,897 (GRCm39)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,552,314 (GRCm39)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,646,735 (GRCm39)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,682,283 (GRCm39)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,624,605 (GRCm39)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,661,475 (GRCm39)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,649,605 (GRCm39)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,715,602 (GRCm39)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,715,701 (GRCm39)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,685,870 (GRCm39)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,664,936 (GRCm39)	missense	probably benign	0.00
R7121:Lama3	UTSW	18	12,595,839 (GRCm39)	missense	probably benign	0.06
R7133:Lama3	UTSW	18	12,672,843 (GRCm39)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,601,346 (GRCm39)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,589,869 (GRCm39)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,537,133 (GRCm39)	missense	probably benign	0.26
R7216:Lama3	UTSW	18	12,563,057 (GRCm39)	missense	probably damaging	1.00
R7223:Lama3	UTSW	18	12,715,665 (GRCm39)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,552,902 (GRCm39)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,572,449 (GRCm39)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,640,097 (GRCm39)	splice site	probably null
R7442:Lama3	UTSW	18	12,605,238 (GRCm39)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,552,294 (GRCm39)	missense	probably benign
R7604:Lama3	UTSW	18	12,633,550 (GRCm39)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,664,891 (GRCm39)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,670,895 (GRCm39)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,595,864 (GRCm39)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,670,796 (GRCm39)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,667,120 (GRCm39)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,639,999 (GRCm39)	missense	probably null
R8219:Lama3	UTSW	18	12,572,417 (GRCm39)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8229:Lama3	UTSW	18	12,540,608 (GRCm39)	missense	probably benign
R8298:Lama3	UTSW	18	12,658,910 (GRCm39)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,673,670 (GRCm39)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,661,404 (GRCm39)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,582,896 (GRCm39)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,544,688 (GRCm39)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,554,212 (GRCm39)	missense	probably benign
R8799:Lama3	UTSW	18	12,624,000 (GRCm39)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,582,643 (GRCm39)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,689,762 (GRCm39)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,665,096 (GRCm39)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,614,120 (GRCm39)	nonsense	probably null
R9126:Lama3	UTSW	18	12,583,527 (GRCm39)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,605,297 (GRCm39)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,595,869 (GRCm39)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,710,959 (GRCm39)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,583,541 (GRCm39)	nonsense	probably null
R9553:Lama3	UTSW	18	12,563,019 (GRCm39)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,583,460 (GRCm39)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,682,320 (GRCm39)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,715,631 (GRCm39)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,562,936 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- CGCATGAAGTAATTGCTTCTTGG -3'
(R):5'- CCCAAATCTGTCTGGATGGG -3'

Sequencing Primer
(F):5'- GAAGTAATTGCTTCTTGGTGTCTCCC -3'
(R):5'- CAAATCTGTCTGGATGGGGTTGAG -3'

Posted On

2016-06-06