Incidental Mutation 'R5023:Klf11'
ID 391156
Institutional Source Beutler Lab
Gene Symbol Klf11
Ensembl Gene ENSMUSG00000020653
Gene Name Kruppel-like factor 11
Synonyms Tieg2b, D12Ertd427e, Tieg3, Tieg2
MMRRC Submission 042614-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5023 (G1)
Quality Score 225
Status Validated
Chromosome 12
Chromosomal Location 24651274-24662789 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 24655359 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Serine to Threonine at position 271 (S271T)
Ref Sequence ENSEMBL: ENSMUSP00000020982 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020982] [ENSMUST00000139940] [ENSMUST00000144046] [ENSMUST00000146894]
AlphaFold Q8K1S5
Predicted Effect probably benign
Transcript: ENSMUST00000020982
AA Change: S271T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000020982
Gene: ENSMUSG00000020653
AA Change: S271T

low complexity region 267 275 N/A INTRINSIC
low complexity region 356 367 N/A INTRINSIC
ZnF_C2H2 384 408 5.9e-3 SMART
ZnF_C2H2 414 438 9.22e-5 SMART
ZnF_C2H2 444 466 1.38e-3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000139940
Predicted Effect probably benign
Transcript: ENSMUST00000144046
Predicted Effect probably benign
Transcript: ENSMUST00000146894
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.2%
Validation Efficiency 98% (127/129)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele are viable and fertile, show normal hematopoiesis, growth and development, and display no evidence of increased tumor formation following gamma-irradiation. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 124 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933427D14Rik A T 11: 72,166,755 (GRCm38) H739Q probably benign Het
Abca3 C T 17: 24,374,300 (GRCm38) R224C probably damaging Het
Abca8b A G 11: 109,974,988 (GRCm38) probably null Het
Adam1b C T 5: 121,501,159 (GRCm38) V608M probably damaging Het
Ank2 A G 3: 126,941,871 (GRCm38) probably benign Het
Ankrd17 A C 5: 90,282,868 (GRCm38) L1019R probably damaging Het
Arih1 T C 9: 59,486,232 (GRCm38) N39S unknown Het
Best3 G A 10: 116,988,742 (GRCm38) V38I probably benign Het
Bicc1 A G 10: 70,947,883 (GRCm38) S393P possibly damaging Het
C1d T A 11: 17,266,674 (GRCm38) N135K probably benign Het
Cage1 G T 13: 38,011,411 (GRCm38) S778* probably null Het
Capn7 T A 14: 31,352,426 (GRCm38) V262E probably damaging Het
Ccdc190 A G 1: 169,933,087 (GRCm38) R95G probably damaging Het
Cd163 C T 6: 124,325,288 (GRCm38) T937I probably damaging Het
Cdh18 G A 15: 23,259,666 (GRCm38) S194N probably damaging Het
Ceacam23 A T 7: 17,902,706 (GRCm38) D236V probably damaging Het
Clec4b2 A T 6: 123,200,956 (GRCm38) S77C probably null Het
Ctsw T A 19: 5,466,049 (GRCm38) D237V probably damaging Het
Cyp2c68 G A 19: 39,712,507 (GRCm38) T289I probably benign Het
Cyp4f39 A G 17: 32,481,104 (GRCm38) Y133C probably damaging Het
Dlg5 T C 14: 24,136,622 (GRCm38) E1847G probably damaging Het
Dnah7a C A 1: 53,647,248 (GRCm38) E248* probably null Het
Eef2kmt T A 16: 5,247,599 (GRCm38) D248V probably damaging Het
Ewsr1 T C 11: 5,088,054 (GRCm38) T113A possibly damaging Het
Fbxo4 A G 15: 3,977,756 (GRCm38) probably null Het
Fbxo43 A T 15: 36,162,929 (GRCm38) M44K probably benign Het
Fchsd1 A G 18: 37,964,810 (GRCm38) I340T possibly damaging Het
Fgf5 C A 5: 98,262,015 (GRCm38) A141E probably damaging Het
Gfm1 T C 3: 67,473,544 (GRCm38) V664A probably damaging Het
Glp2r T C 11: 67,741,032 (GRCm38) T121A possibly damaging Het
Gm10803 A C 2: 93,564,172 (GRCm38) L96F probably damaging Het
Gm14569 T C X: 36,430,817 (GRCm38) D1413G probably benign Het
Gm15455 T C 1: 33,837,351 (GRCm38) noncoding transcript Het
Gm1818 G C 12: 48,555,535 (GRCm38) noncoding transcript Het
Gm7030 A G 17: 36,109,415 (GRCm38) probably benign Het
Gnb2 G A 5: 137,529,940 (GRCm38) probably null Het
Gpc4 G A X: 52,074,563 (GRCm38) R148C probably damaging Het
Gpx5 C T 13: 21,288,745 (GRCm38) V140I probably damaging Het
Gtpbp1 A T 15: 79,719,221 (GRCm38) Q637L possibly damaging Het
Herc1 A T 9: 66,470,326 (GRCm38) K3458M possibly damaging Het
Hhat A T 1: 192,727,339 (GRCm38) L138Q probably damaging Het
Hipk1 G T 3: 103,777,507 (GRCm38) T264N probably damaging Het
Hjurp A T 1: 88,275,050 (GRCm38) Y71N possibly damaging Het
Hnf4g G T 3: 3,644,587 (GRCm38) A144S probably damaging Het
Hook3 TAGAG TAG 8: 26,032,019 (GRCm38) probably null Het
Ifi204 G A 1: 173,751,740 (GRCm38) T513I possibly damaging Het
Ino80b G T 6: 83,125,042 (GRCm38) S26R probably damaging Het
Ints9 T A 14: 64,980,228 (GRCm38) L68H probably damaging Het
Isx C T 8: 74,892,714 (GRCm38) T178I probably benign Het
Kel A G 6: 41,688,111 (GRCm38) L255P probably damaging Het
Klhl20 A C 1: 161,109,220 (GRCm38) probably null Het
Lama2 G A 10: 27,190,504 (GRCm38) T1127I probably damaging Het
Larp1b T A 3: 41,033,985 (GRCm38) N81K possibly damaging Het
Lsm11 T C 11: 45,944,839 (GRCm38) D25G probably damaging Het
Map3k20 T A 2: 72,402,345 (GRCm38) probably benign Het
Mbd6 C T 10: 127,286,441 (GRCm38) V173I probably benign Het
Milr1 A G 11: 106,766,965 (GRCm38) D131G possibly damaging Het
Mybpc1 T G 10: 88,543,774 (GRCm38) D635A probably damaging Het
Myh7b G C 2: 155,632,373 (GRCm38) R1669S possibly damaging Het
Ndufa7 A G 17: 33,824,603 (GRCm38) probably benign Het
Ndufs3 C A 2: 90,898,660 (GRCm38) A161S probably benign Het
Neo1 T C 9: 58,990,271 (GRCm38) D134G probably damaging Het
Nme4 G A 17: 26,093,668 (GRCm38) T129I probably benign Het
Npffr2 A T 5: 89,582,687 (GRCm38) T159S probably benign Het
Nup153 A T 13: 46,681,109 (GRCm38) probably benign Het
Or3a10 T A 11: 74,045,055 (GRCm38) D73V probably damaging Het
Or51ac3 A C 7: 103,565,171 (GRCm38) M36R possibly damaging Het
Pate2 T C 9: 35,686,111 (GRCm38) probably benign Het
Pcca A G 14: 122,790,398 (GRCm38) N73D probably damaging Het
Pip5k1c G A 10: 81,310,889 (GRCm38) probably null Het
Pkhd1l1 A T 15: 44,528,191 (GRCm38) H1551L probably benign Het
Pkhd1l1 G A 15: 44,582,227 (GRCm38) M3768I probably benign Het
Ppa2 A G 3: 133,370,434 (GRCm38) M275V probably benign Het
Ptpn23 G A 9: 110,388,556 (GRCm38) T744I probably benign Het
Ptprv A T 1: 135,124,506 (GRCm38) noncoding transcript Het
Rad17 A T 13: 100,645,063 (GRCm38) H75Q possibly damaging Het
Rbm44 T C 1: 91,169,098 (GRCm38) probably null Het
Rbpj C T 5: 53,649,415 (GRCm38) R201W probably damaging Het
Rbpjl C A 2: 164,410,289 (GRCm38) L215I probably damaging Het
Ror1 A G 4: 100,425,932 (GRCm38) E398G probably benign Het
Scamp3 T A 3: 89,182,293 (GRCm38) probably benign Het
Selenot CATGTATG CATGTATGTATG 3: 58,588,453 (GRCm38) probably null Het
Siglec15 A G 18: 78,048,675 (GRCm38) C104R probably damaging Het
Sis T C 3: 72,934,122 (GRCm38) I787V probably benign Het
Slc17a8 T C 10: 89,576,560 (GRCm38) D521G probably benign Het
Slc7a10 T A 7: 35,197,355 (GRCm38) M172K possibly damaging Het
Slco4c1 G A 1: 96,841,228 (GRCm38) P303L probably damaging Het
Slitrk3 T C 3: 73,050,648 (GRCm38) T264A probably benign Het
Smg8 A C 11: 87,086,137 (GRCm38) V206G probably damaging Het
Smg9 A G 7: 24,405,872 (GRCm38) K137R possibly damaging Het
Srrm2 C A 17: 23,819,317 (GRCm38) probably benign Het
Sult2a2 T A 7: 13,734,860 (GRCm38) Y84N possibly damaging Het
Syngr2 A T 11: 117,812,510 (GRCm38) I34F probably benign Het
Tank G A 2: 61,578,635 (GRCm38) probably benign Het
Tead4 T C 6: 128,294,171 (GRCm38) probably benign Het
Tesl1 G A X: 23,907,241 (GRCm38) G327E probably damaging Het
Tex36 A T 7: 133,595,290 (GRCm38) C33S probably benign Het
Timd5 T A 11: 46,528,532 (GRCm38) D58E probably damaging Het
Timm21 C A 18: 84,949,414 (GRCm38) V112L possibly damaging Het
Tmem240 T A 4: 155,739,674 (GRCm38) L92Q probably damaging Het
Tmem268 C G 4: 63,568,540 (GRCm38) S100C probably damaging Het
Tmod3 G A 9: 75,511,206 (GRCm38) P183S probably damaging Het
Trappc10 G T 10: 78,217,160 (GRCm38) F260L possibly damaging Het
Trim16 A T 11: 62,836,812 (GRCm38) Y233F probably benign Het
Trmt112 T C 19: 6,910,753 (GRCm38) V91A probably benign Het
Ttc3 A G 16: 94,429,359 (GRCm38) E450G probably benign Het
Ttk A G 9: 83,863,541 (GRCm38) D647G probably damaging Het
Ubash3b A G 9: 41,037,459 (GRCm38) C187R possibly damaging Het
Vmn1r29 T A 6: 58,308,067 (GRCm38) Y257* probably null Het
Vmn1r33 T C 6: 66,612,105 (GRCm38) N155S probably benign Het
Vmn2r28 T A 7: 5,486,464 (GRCm38) I459L probably benign Het
Vps16 A G 2: 130,439,452 (GRCm38) S235G probably benign Het
Vwde T C 6: 13,192,642 (GRCm38) I421V possibly damaging Het
Xiap T C X: 42,094,465 (GRCm38) F23L probably benign Het
Xkr7 A G 2: 153,054,380 (GRCm38) T385A probably benign Het
Zbtb11 A G 16: 56,006,065 (GRCm38) Y819C probably damaging Het
Zfp112 A G 7: 24,126,484 (GRCm38) T624A probably damaging Het
Zfp592 A G 7: 81,024,347 (GRCm38) D353G probably damaging Het
Zfp62 T A 11: 49,215,729 (GRCm38) S216T probably damaging Het
Zfp677 A T 17: 21,397,794 (GRCm38) H371L probably damaging Het
Zfp780b T C 7: 27,963,448 (GRCm38) K561E possibly damaging Het
Zfp936 G A 7: 43,187,257 (GRCm38) D31N probably damaging Het
Znfx1 T C 2: 167,039,826 (GRCm38) Y217C probably damaging Het
Zpbp C T 11: 11,415,248 (GRCm38) E200K probably benign Het
Other mutations in Klf11
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01630:Klf11 APN 12 24,660,369 (GRCm38) missense probably benign 0.01
IGL02202:Klf11 APN 12 24,653,632 (GRCm38) missense probably benign 0.37
IGL02527:Klf11 APN 12 24,655,323 (GRCm38) missense probably benign 0.31
IGL02964:Klf11 APN 12 24,655,627 (GRCm38) missense probably damaging 1.00
R0254:Klf11 UTSW 12 24,653,583 (GRCm38) missense probably damaging 1.00
R0553:Klf11 UTSW 12 24,655,090 (GRCm38) missense probably benign 0.12
R0739:Klf11 UTSW 12 24,660,248 (GRCm38) missense probably damaging 1.00
R1584:Klf11 UTSW 12 24,655,305 (GRCm38) missense probably damaging 1.00
R1592:Klf11 UTSW 12 24,653,738 (GRCm38) missense probably damaging 1.00
R2356:Klf11 UTSW 12 24,653,583 (GRCm38) missense probably damaging 1.00
R3085:Klf11 UTSW 12 24,655,491 (GRCm38) missense probably benign
R4690:Klf11 UTSW 12 24,655,072 (GRCm38) missense probably damaging 0.97
R5483:Klf11 UTSW 12 24,655,411 (GRCm38) nonsense probably null
R5528:Klf11 UTSW 12 24,654,930 (GRCm38) missense probably benign 0.00
R6148:Klf11 UTSW 12 24,651,568 (GRCm38) critical splice donor site probably null
R6698:Klf11 UTSW 12 24,653,619 (GRCm38) missense probably damaging 1.00
R6799:Klf11 UTSW 12 24,655,639 (GRCm38) missense possibly damaging 0.59
R7317:Klf11 UTSW 12 24,655,519 (GRCm38) missense possibly damaging 0.59
R7384:Klf11 UTSW 12 24,653,743 (GRCm38) missense probably damaging 0.97
R7440:Klf11 UTSW 12 24,655,491 (GRCm38) missense probably benign
R7473:Klf11 UTSW 12 24,655,142 (GRCm38) splice site probably null
R7477:Klf11 UTSW 12 24,653,563 (GRCm38) missense probably benign 0.01
R7658:Klf11 UTSW 12 24,653,671 (GRCm38) missense probably damaging 1.00
R9378:Klf11 UTSW 12 24,655,044 (GRCm38) missense probably benign 0.01
R9479:Klf11 UTSW 12 24,655,030 (GRCm38) missense probably benign 0.10
R9663:Klf11 UTSW 12 24,655,732 (GRCm38) missense probably damaging 1.00
R9721:Klf11 UTSW 12 24,660,241 (GRCm38) missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2016-06-06