Mutagenetix > Incidental Mutation

Incidental Mutation 'R5024:Fbxw25'

391242

Institutional Source

Beutler Lab

Gene Symbol

Fbxw25

Ensembl Gene

ENSMUSG00000094992

Gene Name

F-box and WD-40 domain protein 25

Synonyms

E330001B16Rik

MMRRC Submission

042615-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R5024 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109645122-109664652 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to T at 109663374 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000128652 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163839]

AlphaFold

F7C9P2

Predicted Effect

probably null
Transcript: ENSMUST00000163839

SMART Domains

Protein: ENSMUSP00000128652
Gene: ENSMUSG00000094992

Domain	Start	End	E-Value	Type
FBOX	5	45	5.44e-6	SMART
SCOP:d1gxra_	119	228	1e-6	SMART
Blast:WD40	137	176	6e-6	BLAST

Meta Mutation Damage Score

0.9343

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.4%

Validation Efficiency

99% (95/96)

Allele List at MGI

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrd1	A	G	5: 129,171,895	N575S	probably damaging	Het
Akap6	C	T	12: 53,142,562	T2253M	probably benign	Het
Arhgef37	A	C	18: 61,506,440	N289K	probably damaging	Het
Armc4	T	C	18: 7,088,555	M1005V	probably benign	Het
Atad2b	A	C	12: 4,937,534	T121P	probably benign	Het
Atp4a	A	C	7: 30,715,864	D303A	possibly damaging	Het
BC005561	A	G	5: 104,522,258	K1549E	possibly damaging	Het
Calu	A	T	6: 29,374,519		probably benign	Het
Ccdc141	A	C	2: 77,054,703	N531K	probably benign	Het
Ccdc146	T	C	5: 21,399,614		probably null	Het
Cd207	G	A	6: 83,674,319	T218I	probably damaging	Het
Cd2ap	A	C	17: 42,805,345		probably null	Het
Clip3	G	A	7: 30,292,219		probably benign	Het
Clstn1	G	A	4: 149,635,294	R432H	possibly damaging	Het
Csmd2	A	G	4: 128,321,348	Y521C	possibly damaging	Het
Dnah8	A	T	17: 30,736,096	E2033V	probably damaging	Het
Eng	T	G	2: 32,673,392	V319G	probably benign	Het
Erp44	C	T	4: 48,241,296	W57*	probably null	Het
Etv1	T	A	12: 38,854,234		probably null	Het
Eva1c	T	C	16: 90,876,193		probably null	Het
Fam196a	A	G	7: 134,918,478	S108P	probably damaging	Het
Fam221b	T	A	4: 43,659,674	N482I	probably damaging	Het
Fam83h	T	C	15: 76,005,142	H202R	probably damaging	Het
Fbxw13	T	C	9: 109,179,335	T449A	probably benign	Het
Frmd3	T	A	4: 74,098,144	S99T	probably benign	Het
Gm5155	A	G	7: 17,910,682	I575V	probably benign	Het
Gm5174	G	T	10: 86,656,587		noncoding transcript	Het
Gm6904	T	C	14: 59,258,483		probably null	Het
Gm815	C	T	19: 26,887,775	Q49*	probably null	Het
H2-DMa	A	T	17: 34,138,487	I245F	possibly damaging	Het
Herc1	A	T	9: 66,470,326	K3458M	possibly damaging	Het
Hirip3	A	G	7: 126,864,489		probably null	Het
Hjurp	A	T	1: 88,275,050	Y71N	possibly damaging	Het
Hmcn1	T	A	1: 150,680,688	E2449V	possibly damaging	Het
Igll1	G	T	16: 16,863,793	H33N	probably benign	Het
Il6	T	C	5: 30,019,514	L184P	probably damaging	Het
Impg2	T	A	16: 56,260,100	S756T	probably damaging	Het
Kank4	T	G	4: 98,785,661	D5A	probably damaging	Het
Kcna7	G	A	7: 45,406,591	R77H	probably damaging	Het
Kcns2	A	T	15: 34,839,537	T349S	probably benign	Het
Keap1	A	G	9: 21,237,226	Y162H	probably damaging	Het
Kif9	T	C	9: 110,483,093	F10L	possibly damaging	Het
Klhdc8b	ACACGCACGCACGCACGCACGCACGCACGCACGCACGCAC	ACACGCACGCACGCACGCACGCACGCACGCACGCACGCACGCAC	9: 108,448,985		probably benign	Het
Klk14	G	A	7: 43,692,077	C51Y	probably damaging	Het
Lpar6	A	G	14: 73,239,369	T257A	probably damaging	Het
Lpin1	A	T	12: 16,554,006	L608Q	probably benign	Het
Lyst	T	C	13: 13,634,404	S220P	probably benign	Het
M1ap	A	G	6: 83,028,358		probably benign	Het
Mbd6	C	T	10: 127,286,441	V173I	probably benign	Het
Myo5b	A	T	18: 74,716,034	T1115S	possibly damaging	Het
Mysm1	C	A	4: 94,951,016	V683F	possibly damaging	Het
Nlrp4g	T	A	9: 124,350,155		noncoding transcript	Het
Olfr1040	G	T	2: 86,146,533	A67E	probably damaging	Het
Olfr1062	C	T	2: 86,423,461	G72S	possibly damaging	Het
Olfr292	A	T	7: 86,694,881	M142L	probably benign	Het
Olfr318	T	A	11: 58,720,950	I33F	probably benign	Het
Otud6b	T	A	4: 14,826,293	Q34L	probably damaging	Het
Parp11	C	T	6: 127,471,636	T72I	probably damaging	Het
Pbx1	T	A	1: 168,183,589	D343V	possibly damaging	Het
Ppp1r12b	G	T	1: 134,955,733	A17E	probably benign	Het
Pramef20	C	A	4: 144,373,308	E296*	probably null	Het
Ranbp9	A	T	13: 43,434,855	I67N	probably damaging	Het
Rasgrp4	A	G	7: 29,148,407	E414G	probably damaging	Het
Rbbp5	A	G	1: 132,490,488	H15R	possibly damaging	Het
Scd2	A	G	19: 44,301,271	Y235C	probably benign	Het
Sdr16c5	C	T	4: 4,010,365	G170S	probably damaging	Het
Sh3bp4	G	T	1: 89,145,595	G722C	probably damaging	Het
Shmt1	A	T	11: 60,797,479		probably benign	Het
Slc12a1	A	G	2: 125,166,137	I206V	probably benign	Het
Slc26a3	A	G	12: 31,453,908	D304G	probably benign	Het
Slc26a7	T	A	4: 14,532,572	D434V	possibly damaging	Het
Slc6a16	G	T	7: 45,259,966	M185I	probably benign	Het
Stat4	A	G	1: 52,082,570	I363V	possibly damaging	Het
Tgfb1i1	G	T	7: 128,248,217	M1I	probably null	Het
Tmem225	T	C	9: 40,149,343	V66A	probably benign	Het
Tmtc4	T	C	14: 122,941,302		probably null	Het
Trpc4	T	A	3: 54,194,796	N38K	probably benign	Het
Ttll12	A	T	15: 83,587,113	Y218N	probably damaging	Het
Ttn	A	T	2: 76,948,425		probably null	Het
Tulp1	A	C	17: 28,351,995	Y178*	probably null	Het
Vmn2r58	A	G	7: 41,864,322	V299A	probably damaging	Het
Washc4	C	A	10: 83,583,336	Q911K	possibly damaging	Het
Wdr3	T	C	3: 100,154,936	D221G	probably benign	Het
Zan	A	T	5: 137,461,893	C1245*	probably null	Het
Zfyve9	A	C	4: 108,691,669	S773A	probably benign	Het

Other mutations in Fbxw25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03330:Fbxw25	APN	9	109645239	missense	probably benign	0.00
doughnuts	UTSW	9	109650064	missense
goodtimes	UTSW	9	109663374	critical splice donor site	probably null
shakeys	UTSW	9	109654583	missense
R0158:Fbxw25	UTSW	9	109654652	missense	possibly damaging	0.74
R0850:Fbxw25	UTSW	9	109649617	missense	probably benign
R1109:Fbxw25	UTSW	9	109650060	missense	probably benign	0.00
R1386:Fbxw25	UTSW	9	109654641	missense	possibly damaging	0.77
R1609:Fbxw25	UTSW	9	109663510	missense	probably benign	0.11
R1750:Fbxw25	UTSW	9	109650073	missense	probably benign	0.23
R1977:Fbxw25	UTSW	9	109652856	missense	possibly damaging	0.72
R2427:Fbxw25	UTSW	9	109652860	missense	probably benign	0.09
R3841:Fbxw25	UTSW	9	109662134	nonsense	probably null
R4356:Fbxw25	UTSW	9	109662085	missense	probably damaging	1.00
R4934:Fbxw25	UTSW	9	109651637	missense	possibly damaging	0.63
R5175:Fbxw25	UTSW	9	109664563	missense	probably damaging	1.00
R5323:Fbxw25	UTSW	9	109663505	missense	probably benign	0.04
R5389:Fbxw25	UTSW	9	109652886	missense	possibly damaging	0.95
R5493:Fbxw25	UTSW	9	109652916	missense	probably benign	0.01
R6268:Fbxw25	UTSW	9	109654650	missense	probably damaging	1.00
R6739:Fbxw25	UTSW	9	109651631	missense	probably benign	0.29
R7275:Fbxw25	UTSW	9	109654592	missense
R7492:Fbxw25	UTSW	9	109664530	critical splice donor site	probably null
R7623:Fbxw25	UTSW	9	109654583	missense
R7784:Fbxw25	UTSW	9	109650119	missense
R7861:Fbxw25	UTSW	9	109664557	nonsense	probably null
R7887:Fbxw25	UTSW	9	109649594	critical splice donor site	probably null
R8973:Fbxw25	UTSW	9	109650064	missense
R9517:Fbxw25	UTSW	9	109651824	missense
R9563:Fbxw25	UTSW	9	109654608	missense
R9565:Fbxw25	UTSW	9	109654608	missense
X0023:Fbxw25	UTSW	9	109651775	missense	possibly damaging	0.94
Z1176:Fbxw25	UTSW	9	109651738	missense

Predicted Primers

PCR Primer
(F):5'- ACAATGTTGAGCAACCACTCAG -3'
(R):5'- AACCATGGCTCTAAGGTGAGG -3'

Sequencing Primer
(F):5'- GCAGATCACAGATTGACTCCATGG -3'
(R):5'- CAACTGTGCATTGCAGGAAATTGTG -3'

Posted On

2016-06-06