Mutagenetix > Incidental Mutation

Incidental Mutation 'R5024:Lyst'

391256

Institutional Source

Beutler Lab

Gene Symbol

Lyst

Ensembl Gene

ENSMUSG00000019726

Gene Name

lysosomal trafficking regulator

Synonyms

D13Sfk13

MMRRC Submission

042615-MU

Accession Numbers

Ncbi RefSeq: NM_010748.2; MGI:107448

Essential gene?

Possibly non essential (E-score: 0.298) question?

Stock #

R5024 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

13590397-13778803 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 13634404 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 220 (S220P)

Ref Sequence

ENSEMBL: ENSMUSP00000106188 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110559]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000110559
AA Change: S220P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000106188
Gene: ENSMUSG00000019726
AA Change: S220P

Domain	Start	End	E-Value	Type
low complexity region	26	36	N/A	INTRINSIC
low complexity region	72	82	N/A	INTRINSIC
low complexity region	399	412	N/A	INTRINSIC
low complexity region	1333	1344	N/A	INTRINSIC
low complexity region	2295	2307	N/A	INTRINSIC
low complexity region	2427	2445	N/A	INTRINSIC
low complexity region	2534	2546	N/A	INTRINSIC
Pfam:PH_BEACH	3006	3101	5.8e-25	PFAM
Beach	3118	3408	1.25e-193	SMART
Blast:Beach	3441	3478	9e-13	BLAST
WD40	3539	3579	5.75e-1	SMART
WD40	3591	3630	2.89e-5	SMART
WD40	3633	3676	1.38e0	SMART
WD40	3724	3765	1.27e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000220937

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000223053

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.4%

Validation Efficiency

99% (95/96)

MGI Phenotype

Strain: 1855968
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that regulates intracellular protein trafficking in endosomes, and may be involved in pigmentation. Mutations in this gene are associated with Chediak-Higashi syndrome, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants, though the full-length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2013]
PHENOTYPE: Homozygous mice have a phenotype similar to human Chediak-Higashi syndrome patients, exhibiting lysosomal dysfunction with resultant protein storage; diluted coat color; abnormal melanogenesis; immune cell dysfunction resulting in increased susceptibility to bacterial, viral, and parasitic infections and decreased cytotoxic activity against tumor cells. [provided by MGI curators]

Allele List at MGI

All alleles(52) : Targeted(3) Gene trapped(34) Spontaneous(8) Chemically induced(6) Radiation induced(1)

Other mutations in this stock

Total: 85 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrd1	A	G	5: 129,171,895 (GRCm38)	N575S	probably damaging	Het
Akap6	C	T	12: 53,142,562 (GRCm38)	T2253M	probably benign	Het
Arhgef37	A	C	18: 61,506,440 (GRCm38)	N289K	probably damaging	Het
Armc4	T	C	18: 7,088,555 (GRCm38)	M1005V	probably benign	Het
Atad2b	A	C	12: 4,937,534 (GRCm38)	T121P	probably benign	Het
Atp4a	A	C	7: 30,715,864 (GRCm38)	D303A	possibly damaging	Het
BC005561	A	G	5: 104,522,258 (GRCm38)	K1549E	possibly damaging	Het
Calu	A	T	6: 29,374,519 (GRCm38)		probably benign	Het
Ccdc141	A	C	2: 77,054,703 (GRCm38)	N531K	probably benign	Het
Ccdc146	T	C	5: 21,399,614 (GRCm38)		probably null	Het
Cd207	G	A	6: 83,674,319 (GRCm38)	T218I	probably damaging	Het
Cd2ap	A	C	17: 42,805,345 (GRCm38)		probably null	Het
Clip3	G	A	7: 30,292,219 (GRCm38)		probably benign	Het
Clstn1	G	A	4: 149,635,294 (GRCm38)	R432H	possibly damaging	Het
Csmd2	A	G	4: 128,321,348 (GRCm38)	Y521C	possibly damaging	Het
Dnah8	A	T	17: 30,736,096 (GRCm38)	E2033V	probably damaging	Het
Eng	T	G	2: 32,673,392 (GRCm38)	V319G	probably benign	Het
Erp44	C	T	4: 48,241,296 (GRCm38)	W57*	probably null	Het
Etv1	T	A	12: 38,854,234 (GRCm38)		probably null	Het
Eva1c	T	C	16: 90,876,193 (GRCm38)		probably null	Het
Fam196a	A	G	7: 134,918,478 (GRCm38)	S108P	probably damaging	Het
Fam221b	T	A	4: 43,659,674 (GRCm38)	N482I	probably damaging	Het
Fam83h	T	C	15: 76,005,142 (GRCm38)	H202R	probably damaging	Het
Fbxw13	T	C	9: 109,179,335 (GRCm38)	T449A	probably benign	Het
Fbxw25	A	T	9: 109,663,374 (GRCm38)		probably null	Het
Frmd3	T	A	4: 74,098,144 (GRCm38)	S99T	probably benign	Het
Gm5155	A	G	7: 17,910,682 (GRCm38)	I575V	probably benign	Het
Gm5174	G	T	10: 86,656,587 (GRCm38)		noncoding transcript	Het
Gm6904	T	C	14: 59,258,483 (GRCm38)		probably null	Het
Gm815	C	T	19: 26,887,775 (GRCm38)	Q49*	probably null	Het
H2-DMa	A	T	17: 34,138,487 (GRCm38)	I245F	possibly damaging	Het
Herc1	A	T	9: 66,470,326 (GRCm38)	K3458M	possibly damaging	Het
Hirip3	A	G	7: 126,864,489 (GRCm38)		probably null	Het
Hjurp	A	T	1: 88,275,050 (GRCm38)	Y71N	possibly damaging	Het
Hmcn1	T	A	1: 150,680,688 (GRCm38)	E2449V	possibly damaging	Het
Igll1	G	T	16: 16,863,793 (GRCm38)	H33N	probably benign	Het
Il6	T	C	5: 30,019,514 (GRCm38)	L184P	probably damaging	Het
Impg2	T	A	16: 56,260,100 (GRCm38)	S756T	probably damaging	Het
Kank4	T	G	4: 98,785,661 (GRCm38)	D5A	probably damaging	Het
Kcna7	G	A	7: 45,406,591 (GRCm38)	R77H	probably damaging	Het
Kcns2	A	T	15: 34,839,537 (GRCm38)	T349S	probably benign	Het
Keap1	A	G	9: 21,237,226 (GRCm38)	Y162H	probably damaging	Het
Kif9	T	C	9: 110,483,093 (GRCm38)	F10L	possibly damaging	Het
Klhdc8b	ACACGCACGCACGCACGCACGCACGCACGCACGCACGCAC	ACACGCACGCACGCACGCACGCACGCACGCACGCACGCACGCAC	9: 108,448,985 (GRCm38)		probably benign	Het
Klk14	G	A	7: 43,692,077 (GRCm38)	C51Y	probably damaging	Het
Lpar6	A	G	14: 73,239,369 (GRCm38)	T257A	probably damaging	Het
Lpin1	A	T	12: 16,554,006 (GRCm38)	L608Q	probably benign	Het
M1ap	A	G	6: 83,028,358 (GRCm38)		probably benign	Het
Mbd6	C	T	10: 127,286,441 (GRCm38)	V173I	probably benign	Het
Myo5b	A	T	18: 74,716,034 (GRCm38)	T1115S	possibly damaging	Het
Mysm1	C	A	4: 94,951,016 (GRCm38)	V683F	possibly damaging	Het
Nlrp4g	T	A	9: 124,350,155 (GRCm38)		noncoding transcript	Het
Olfr1040	G	T	2: 86,146,533 (GRCm38)	A67E	probably damaging	Het
Olfr1062	C	T	2: 86,423,461 (GRCm38)	G72S	possibly damaging	Het
Olfr292	A	T	7: 86,694,881 (GRCm38)	M142L	probably benign	Het
Olfr318	T	A	11: 58,720,950 (GRCm38)	I33F	probably benign	Het
Otud6b	T	A	4: 14,826,293 (GRCm38)	Q34L	probably damaging	Het
Parp11	C	T	6: 127,471,636 (GRCm38)	T72I	probably damaging	Het
Pbx1	T	A	1: 168,183,589 (GRCm38)	D343V	possibly damaging	Het
Ppp1r12b	G	T	1: 134,955,733 (GRCm38)	A17E	probably benign	Het
Pramef20	C	A	4: 144,373,308 (GRCm38)	E296*	probably null	Het
Ranbp9	A	T	13: 43,434,855 (GRCm38)	I67N	probably damaging	Het
Rasgrp4	A	G	7: 29,148,407 (GRCm38)	E414G	probably damaging	Het
Rbbp5	A	G	1: 132,490,488 (GRCm38)	H15R	possibly damaging	Het
Scd2	A	G	19: 44,301,271 (GRCm38)	Y235C	probably benign	Het
Sdr16c5	C	T	4: 4,010,365 (GRCm38)	G170S	probably damaging	Het
Sh3bp4	G	T	1: 89,145,595 (GRCm38)	G722C	probably damaging	Het
Shmt1	A	T	11: 60,797,479 (GRCm38)		probably benign	Het
Slc12a1	A	G	2: 125,166,137 (GRCm38)	I206V	probably benign	Het
Slc26a3	A	G	12: 31,453,908 (GRCm38)	D304G	probably benign	Het
Slc26a7	T	A	4: 14,532,572 (GRCm38)	D434V	possibly damaging	Het
Slc6a16	G	T	7: 45,259,966 (GRCm38)	M185I	probably benign	Het
Stat4	A	G	1: 52,082,570 (GRCm38)	I363V	possibly damaging	Het
Tgfb1i1	G	T	7: 128,248,217 (GRCm38)	M1I	probably null	Het
Tmem225	T	C	9: 40,149,343 (GRCm38)	V66A	probably benign	Het
Tmtc4	T	C	14: 122,941,302 (GRCm38)		probably null	Het
Trpc4	T	A	3: 54,194,796 (GRCm38)	N38K	probably benign	Het
Ttll12	A	T	15: 83,587,113 (GRCm38)	Y218N	probably damaging	Het
Ttn	A	T	2: 76,948,425 (GRCm38)		probably null	Het
Tulp1	A	C	17: 28,351,995 (GRCm38)	Y178*	probably null	Het
Vmn2r58	A	G	7: 41,864,322 (GRCm38)	V299A	probably damaging	Het
Washc4	C	A	10: 83,583,336 (GRCm38)	Q911K	possibly damaging	Het
Wdr3	T	C	3: 100,154,936 (GRCm38)	D221G	probably benign	Het
Zan	A	T	5: 137,461,893 (GRCm38)	C1245*	probably null	Het
Zfyve9	A	C	4: 108,691,669 (GRCm38)	S773A	probably benign	Het

Other mutations in Lyst

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Lyst	APN	13	13,648,878 (GRCm38)	missense	probably benign
IGL00474:Lyst	APN	13	13,643,536 (GRCm38)	missense	possibly damaging	0.48
IGL00484:Lyst	APN	13	13,709,603 (GRCm38)	missense	probably benign	0.02
IGL00492:Lyst	APN	13	13,678,175 (GRCm38)	missense	possibly damaging	0.54
IGL00807:Lyst	APN	13	13,650,423 (GRCm38)	missense	possibly damaging	0.91
IGL00949:Lyst	APN	13	13,635,485 (GRCm38)	missense	possibly damaging	0.87
IGL00952:Lyst	APN	13	13,678,107 (GRCm38)	missense	probably benign	0.05
IGL01305:Lyst	APN	13	13,678,056 (GRCm38)	missense	probably benign	0.01
IGL01317:Lyst	APN	13	13,670,870 (GRCm38)	missense	probably benign
IGL01419:Lyst	APN	13	13,635,838 (GRCm38)	missense	probably benign	0.00
IGL01445:Lyst	APN	13	13,651,714 (GRCm38)	missense	probably benign	0.00
IGL01690:Lyst	APN	13	13,743,246 (GRCm38)	missense	probably damaging	1.00
IGL01791:Lyst	APN	13	13,635,302 (GRCm38)	missense	probably damaging	1.00
IGL01809:Lyst	APN	13	13,637,803 (GRCm38)	missense	probably damaging	1.00
IGL01896:Lyst	APN	13	13,635,577 (GRCm38)	missense	probably benign	0.04
IGL01938:Lyst	APN	13	13,637,424 (GRCm38)	missense	possibly damaging	0.93
IGL01986:Lyst	APN	13	13,775,627 (GRCm38)	critical splice donor site	probably null
IGL02022:Lyst	APN	13	13,664,044 (GRCm38)	nonsense	probably null
IGL02044:Lyst	APN	13	13,712,846 (GRCm38)	missense	probably damaging	1.00
IGL02157:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02185:Lyst	APN	13	13,661,093 (GRCm38)	nonsense	probably null
IGL02215:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02245:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02246:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02247:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02297:Lyst	APN	13	13,638,092 (GRCm38)	nonsense	probably null
IGL02411:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02415:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02419:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02420:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02429:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02501:Lyst	APN	13	13,711,645 (GRCm38)	missense	probably benign	0.02
IGL02522:Lyst	APN	13	13,634,705 (GRCm38)	missense	possibly damaging	0.81
IGL02535:Lyst	APN	13	13,650,342 (GRCm38)	missense	probably benign	0.00
IGL02596:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02601:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02603:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02608:Lyst	APN	13	13,712,754 (GRCm38)	missense	probably damaging	0.98
IGL02622:Lyst	APN	13	13,681,390 (GRCm38)	missense	probably damaging	1.00
IGL02690:Lyst	APN	13	13,641,125 (GRCm38)	missense	possibly damaging	0.58
IGL02715:Lyst	APN	13	13,674,320 (GRCm38)	splice site	probably null
IGL02725:Lyst	APN	13	13,760,827 (GRCm38)	missense	probably damaging	1.00
IGL02729:Lyst	APN	13	13,746,609 (GRCm38)	missense	possibly damaging	0.95
IGL02729:Lyst	APN	13	13,674,339 (GRCm38)	missense	possibly damaging	0.81
IGL02820:Lyst	APN	13	13,638,058 (GRCm38)	missense	probably benign	0.03
IGL02945:Lyst	APN	13	13,761,198 (GRCm38)	missense	possibly damaging	0.48
IGL02981:Lyst	APN	13	13,634,911 (GRCm38)	missense	probably damaging	0.99
IGL03087:Lyst	APN	13	13,635,056 (GRCm38)	missense	probably damaging	1.00
IGL03149:Lyst	APN	13	13,681,444 (GRCm38)	missense	probably benign	0.14
IGL03158:Lyst	APN	13	13,651,752 (GRCm38)	critical splice donor site	probably null
IGL03226:Lyst	APN	13	13,709,559 (GRCm38)	missense	probably benign	0.01
IGL03242:Lyst	APN	13	13,656,881 (GRCm38)	nonsense	probably null
IGL03385:Lyst	APN	13	13,656,980 (GRCm38)	nonsense	probably null
50-cal	UTSW	13	13,708,212 (GRCm38)	critical splice donor site	probably null
charcoal	UTSW	13	13,696,761 (GRCm38)	nonsense	probably null
charlotte_gray	UTSW	13	13,602,026 (GRCm38)	intron	probably benign
charzard	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
grey_wolf	UTSW	13	0 ()	unclassified
lightspeed	UTSW	13	13,740,536 (GRCm38)	missense	possibly damaging	0.91
pardon	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
robin	UTSW	13	13,648,802 (GRCm38)	nonsense	probably null
sooty	UTSW	13	0 ()	unclassified
souris	UTSW	13	13,683,223 (GRCm38)	unclassified	probably benign
Swallow	UTSW	13	13,757,422 (GRCm38)	missense	probably benign	0.00
vulpix	UTSW	13	13,696,794 (GRCm38)	splice site	probably null
ANU22:Lyst	UTSW	13	13,678,056 (GRCm38)	missense	probably benign	0.01
IGL02835:Lyst	UTSW	13	13,661,100 (GRCm38)	missense	possibly damaging	0.82
P0031:Lyst	UTSW	13	13,664,031 (GRCm38)	missense	probably damaging	1.00
R0012:Lyst	UTSW	13	13,687,694 (GRCm38)	missense	probably benign	0.10
R0012:Lyst	UTSW	13	13,687,694 (GRCm38)	missense	probably benign	0.10
R0031:Lyst	UTSW	13	13,708,156 (GRCm38)	missense	probably benign	0.14
R0115:Lyst	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
R0212:Lyst	UTSW	13	13,635,985 (GRCm38)	missense	possibly damaging	0.93
R0386:Lyst	UTSW	13	13,708,214 (GRCm38)	splice site	probably benign
R0393:Lyst	UTSW	13	13,647,079 (GRCm38)	missense	probably benign	0.01
R0415:Lyst	UTSW	13	13,711,610 (GRCm38)	splice site	probably benign
R0446:Lyst	UTSW	13	13,638,048 (GRCm38)	missense	probably benign	0.00
R0481:Lyst	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
R0499:Lyst	UTSW	13	13,616,713 (GRCm38)	missense	probably damaging	1.00
R0506:Lyst	UTSW	13	13,638,015 (GRCm38)	missense	probably benign
R0530:Lyst	UTSW	13	13,757,306 (GRCm38)	splice site	probably benign
R0541:Lyst	UTSW	13	13,681,293 (GRCm38)	missense	probably benign	0.00
R0570:Lyst	UTSW	13	13,709,386 (GRCm38)	missense	probably benign	0.26
R0680:Lyst	UTSW	13	13,650,341 (GRCm38)	missense	probably benign	0.01
R0842:Lyst	UTSW	13	13,678,241 (GRCm38)	nonsense	probably null
R0848:Lyst	UTSW	13	13,634,930 (GRCm38)	missense	probably benign	0.00
R1014:Lyst	UTSW	13	13,634,060 (GRCm38)	missense	possibly damaging	0.49
R1205:Lyst	UTSW	13	13,680,202 (GRCm38)	missense	probably benign
R1251:Lyst	UTSW	13	13,634,483 (GRCm38)	missense	probably benign	0.00
R1304:Lyst	UTSW	13	13,751,984 (GRCm38)	nonsense	probably null
R1398:Lyst	UTSW	13	13,740,536 (GRCm38)	missense	possibly damaging	0.91
R1445:Lyst	UTSW	13	13,640,054 (GRCm38)	missense	possibly damaging	0.94
R1475:Lyst	UTSW	13	13,708,212 (GRCm38)	critical splice donor site	probably null
R1479:Lyst	UTSW	13	13,634,482 (GRCm38)	missense	probably benign	0.00
R1484:Lyst	UTSW	13	13,678,190 (GRCm38)	missense	probably benign	0.01
R1498:Lyst	UTSW	13	13,650,375 (GRCm38)	missense	possibly damaging	0.49
R1540:Lyst	UTSW	13	13,635,101 (GRCm38)	missense	possibly damaging	0.81
R1611:Lyst	UTSW	13	13,634,897 (GRCm38)	missense	probably damaging	0.97
R1653:Lyst	UTSW	13	13,635,226 (GRCm38)	missense	probably damaging	1.00
R1669:Lyst	UTSW	13	13,644,087 (GRCm38)	missense	possibly damaging	0.90
R1686:Lyst	UTSW	13	13,634,705 (GRCm38)	missense	possibly damaging	0.81
R1694:Lyst	UTSW	13	13,661,161 (GRCm38)	missense	probably damaging	0.98
R1747:Lyst	UTSW	13	13,757,422 (GRCm38)	missense	probably benign	0.00
R1793:Lyst	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
R1871:Lyst	UTSW	13	13,651,712 (GRCm38)	missense	probably benign	0.00
R1905:Lyst	UTSW	13	13,634,134 (GRCm38)	missense	probably benign
R1958:Lyst	UTSW	13	13,616,618 (GRCm38)	missense	probably damaging	1.00
R1969:Lyst	UTSW	13	13,730,344 (GRCm38)	missense	probably damaging	0.99
R2040:Lyst	UTSW	13	13,641,222 (GRCm38)	missense	probably benign	0.00
R2109:Lyst	UTSW	13	13,712,820 (GRCm38)	missense	possibly damaging	0.46
R2116:Lyst	UTSW	13	13,635,701 (GRCm38)	missense	probably damaging	0.99
R2121:Lyst	UTSW	13	13,660,971 (GRCm38)	missense	probably damaging	1.00
R2127:Lyst	UTSW	13	13,635,262 (GRCm38)	missense	probably damaging	1.00
R2187:Lyst	UTSW	13	13,709,341 (GRCm38)	missense	possibly damaging	0.61
R2238:Lyst	UTSW	13	13,743,263 (GRCm38)	missense	probably benign	0.41
R2258:Lyst	UTSW	13	13,637,658 (GRCm38)	missense	probably benign	0.00
R2292:Lyst	UTSW	13	13,740,495 (GRCm38)	missense	probably damaging	1.00
R2368:Lyst	UTSW	13	13,696,663 (GRCm38)	missense	probably damaging	0.96
R2908:Lyst	UTSW	13	13,669,873 (GRCm38)	missense	probably benign	0.03
R3001:Lyst	UTSW	13	13,696,705 (GRCm38)	missense	probably benign
R3002:Lyst	UTSW	13	13,696,705 (GRCm38)	missense	probably benign
R3024:Lyst	UTSW	13	13,658,687 (GRCm38)	missense	probably benign
R3113:Lyst	UTSW	13	13,669,927 (GRCm38)	missense	probably benign	0.12
R3406:Lyst	UTSW	13	13,635,230 (GRCm38)	missense	possibly damaging	0.56
R3972:Lyst	UTSW	13	13,706,625 (GRCm38)	missense	possibly damaging	0.67
R3978:Lyst	UTSW	13	13,634,168 (GRCm38)	missense	possibly damaging	0.82
R4032:Lyst	UTSW	13	13,616,665 (GRCm38)	missense	probably damaging	1.00
R4192:Lyst	UTSW	13	13,740,513 (GRCm38)	missense	probably damaging	1.00
R4206:Lyst	UTSW	13	13,635,989 (GRCm38)	missense	probably benign	0.03
R4298:Lyst	UTSW	13	13,634,887 (GRCm38)	missense	probably damaging	1.00
R4344:Lyst	UTSW	13	13,698,466 (GRCm38)	missense	probably benign	0.06
R4441:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4445:Lyst	UTSW	13	13,709,564 (GRCm38)	missense	probably benign	0.42
R4477:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4493:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4494:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4495:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4622:Lyst	UTSW	13	13,674,398 (GRCm38)	missense	probably benign	0.01
R4638:Lyst	UTSW	13	13,696,794 (GRCm38)	splice site	probably null
R4658:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4675:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4719:Lyst	UTSW	13	13,650,350 (GRCm38)	missense	probably benign
R4729:Lyst	UTSW	13	13,637,901 (GRCm38)	missense	probably damaging	1.00
R4774:Lyst	UTSW	13	13,740,597 (GRCm38)	missense	probably damaging	1.00
R4811:Lyst	UTSW	13	13,777,100 (GRCm38)	missense	probably benign	0.33
R4877:Lyst	UTSW	13	13,683,149 (GRCm38)	missense	probably damaging	1.00
R4920:Lyst	UTSW	13	13,647,060 (GRCm38)	missense	possibly damaging	0.79
R4933:Lyst	UTSW	13	13,759,378 (GRCm38)	missense	probably benign	0.12
R4933:Lyst	UTSW	13	13,637,764 (GRCm38)	missense	probably damaging	0.98
R4958:Lyst	UTSW	13	13,635,463 (GRCm38)	missense	probably benign	0.00
R4982:Lyst	UTSW	13	13,725,954 (GRCm38)	missense	probably damaging	1.00
R4992:Lyst	UTSW	13	13,661,163 (GRCm38)	missense	probably damaging	1.00
R5049:Lyst	UTSW	13	13,636,064 (GRCm38)	missense	probably damaging	1.00
R5079:Lyst	UTSW	13	13,757,353 (GRCm38)	missense	probably benign	0.08
R5254:Lyst	UTSW	13	13,683,070 (GRCm38)	missense	probably benign	0.00
R5266:Lyst	UTSW	13	13,660,970 (GRCm38)	missense	probably damaging	1.00
R5279:Lyst	UTSW	13	13,648,802 (GRCm38)	nonsense	probably null
R5285:Lyst	UTSW	13	13,634,426 (GRCm38)	missense	probably benign	0.01
R5364:Lyst	UTSW	13	13,656,854 (GRCm38)	missense	probably benign	0.35
R5435:Lyst	UTSW	13	13,777,064 (GRCm38)	missense	possibly damaging	0.64
R5516:Lyst	UTSW	13	13,644,122 (GRCm38)	missense	probably benign	0.10
R5524:Lyst	UTSW	13	13,746,779 (GRCm38)	missense	probably benign	0.03
R5591:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5592:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5593:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5594:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5594:Lyst	UTSW	13	13,759,397 (GRCm38)	missense	probably benign	0.00
R5644:Lyst	UTSW	13	13,637,496 (GRCm38)	missense	possibly damaging	0.58
R5659:Lyst	UTSW	13	13,634,627 (GRCm38)	missense	possibly damaging	0.58
R5741:Lyst	UTSW	13	13,634,030 (GRCm38)	missense	probably benign	0.44
R5908:Lyst	UTSW	13	13,696,761 (GRCm38)	nonsense	probably null
R5969:Lyst	UTSW	13	13,687,813 (GRCm38)	splice site	probably null
R6128:Lyst	UTSW	13	13,759,379 (GRCm38)	missense	possibly damaging	0.67
R6271:Lyst	UTSW	13	13,658,754 (GRCm38)	missense	probably benign	0.30
R6315:Lyst	UTSW	13	13,643,504 (GRCm38)	missense	probably benign
R6318:Lyst	UTSW	13	13,743,311 (GRCm38)	missense	possibly damaging	0.88
R6555:Lyst	UTSW	13	13,648,925 (GRCm38)	missense	probably benign	0.01
R6663:Lyst	UTSW	13	13,664,116 (GRCm38)	splice site	probably null
R6701:Lyst	UTSW	13	13,681,485 (GRCm38)	missense	probably benign	0.06
R6711:Lyst	UTSW	13	13,635,235 (GRCm38)	missense	possibly damaging	0.80
R6909:Lyst	UTSW	13	13,743,375 (GRCm38)	missense	probably damaging	1.00
R6915:Lyst	UTSW	13	13,726,044 (GRCm38)	missense	probably benign	0.01
R6929:Lyst	UTSW	13	13,743,324 (GRCm38)	missense	probably damaging	1.00
R6960:Lyst	UTSW	13	13,634,078 (GRCm38)	missense	probably benign	0.12
R7018:Lyst	UTSW	13	13,743,459 (GRCm38)	critical splice donor site	probably null
R7037:Lyst	UTSW	13	13,616,666 (GRCm38)	missense	probably damaging	1.00
R7045:Lyst	UTSW	13	13,637,708 (GRCm38)	missense	probably damaging	1.00
R7045:Lyst	UTSW	13	13,634,900 (GRCm38)	missense	probably benign	0.34
R7070:Lyst	UTSW	13	13,757,444 (GRCm38)	missense	probably benign	0.23
R7188:Lyst	UTSW	13	13,752,090 (GRCm38)	missense	possibly damaging	0.66
R7201:Lyst	UTSW	13	13,709,300 (GRCm38)	nonsense	probably null
R7210:Lyst	UTSW	13	13,656,983 (GRCm38)	missense	probably damaging	1.00
R7229:Lyst	UTSW	13	13,643,509 (GRCm38)	missense	probably benign	0.00
R7293:Lyst	UTSW	13	13,680,237 (GRCm38)	missense	probably benign	0.01
R7318:Lyst	UTSW	13	13,757,443 (GRCm38)	missense	probably benign	0.13
R7344:Lyst	UTSW	13	13,706,555 (GRCm38)	missense	probably benign
R7426:Lyst	UTSW	13	13,637,524 (GRCm38)	missense	probably benign
R7522:Lyst	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
R7583:Lyst	UTSW	13	13,635,887 (GRCm38)	missense	probably damaging	1.00
R7606:Lyst	UTSW	13	13,637,475 (GRCm38)	missense	probably damaging	1.00
R7636:Lyst	UTSW	13	13,616,747 (GRCm38)	critical splice donor site	probably null
R7658:Lyst	UTSW	13	13,730,476 (GRCm38)	missense	possibly damaging	0.63
R7685:Lyst	UTSW	13	13,669,865 (GRCm38)	missense	probably benign	0.00
R7689:Lyst	UTSW	13	13,683,223 (GRCm38)	critical splice donor site	probably null
R7765:Lyst	UTSW	13	13,709,532 (GRCm38)	missense	possibly damaging	0.75
R7779:Lyst	UTSW	13	13,634,543 (GRCm38)	missense	probably damaging	1.00
R7871:Lyst	UTSW	13	13,636,052 (GRCm38)	nonsense	probably null
R7872:Lyst	UTSW	13	13,635,865 (GRCm38)	missense	probably benign	0.14
R7884:Lyst	UTSW	13	13,707,683 (GRCm38)	missense	probably benign	0.09
R7890:Lyst	UTSW	13	13,740,569 (GRCm38)	missense	probably damaging	0.99
R7916:Lyst	UTSW	13	13,647,072 (GRCm38)	missense	possibly damaging	0.64
R7948:Lyst	UTSW	13	13,746,589 (GRCm38)	missense	possibly damaging	0.59
R7956:Lyst	UTSW	13	13,641,203 (GRCm38)	missense	possibly damaging	0.80
R8048:Lyst	UTSW	13	13,687,645 (GRCm38)	missense	probably benign	0.12
R8085:Lyst	UTSW	13	13,634,309 (GRCm38)	missense	probably damaging	0.98
R8165:Lyst	UTSW	13	13,698,360 (GRCm38)	missense	probably damaging	0.99
R8235:Lyst	UTSW	13	13,760,738 (GRCm38)	missense	possibly damaging	0.69
R8237:Lyst	UTSW	13	13,651,732 (GRCm38)	missense	probably benign	0.00
R8275:Lyst	UTSW	13	13,776,082 (GRCm38)	missense	probably benign	0.02
R8300:Lyst	UTSW	13	13,664,058 (GRCm38)	missense	possibly damaging	0.79
R8350:Lyst	UTSW	13	13,650,388 (GRCm38)	nonsense	probably null
R8526:Lyst	UTSW	13	13,760,806 (GRCm38)	missense	probably damaging	0.99
R8551:Lyst	UTSW	13	13,634,060 (GRCm38)	missense	possibly damaging	0.77
R8723:Lyst	UTSW	13	13,712,757 (GRCm38)	missense	possibly damaging	0.89
R8772:Lyst	UTSW	13	13,637,492 (GRCm38)	nonsense	probably null
R8778:Lyst	UTSW	13	13,728,567 (GRCm38)	missense	possibly damaging	0.89
R8778:Lyst	UTSW	13	13,635,776 (GRCm38)	missense	possibly damaging	0.89
R8801:Lyst	UTSW	13	13,661,010 (GRCm38)	missense	probably benign	0.10
R8837:Lyst	UTSW	13	13,677,963 (GRCm38)	missense	probably benign
R8874:Lyst	UTSW	13	13,637,562 (GRCm38)	missense	probably benign
R8878:Lyst	UTSW	13	13,641,076 (GRCm38)	missense	probably benign	0.00
R8891:Lyst	UTSW	13	13,712,850 (GRCm38)	missense	possibly damaging	0.67
R9077:Lyst	UTSW	13	13,683,108 (GRCm38)	missense	probably benign	0.02
R9127:Lyst	UTSW	13	13,634,242 (GRCm38)	missense	probably damaging	1.00
R9143:Lyst	UTSW	13	13,661,165 (GRCm38)	missense	probably damaging	0.98
R9216:Lyst	UTSW	13	13,648,603 (GRCm38)	missense	probably benign
R9217:Lyst	UTSW	13	13,696,660 (GRCm38)	missense	probably benign	0.01
R9291:Lyst	UTSW	13	13,709,353 (GRCm38)	missense	probably benign	0.01
R9302:Lyst	UTSW	13	13,730,362 (GRCm38)	missense	possibly damaging	0.46
R9370:Lyst	UTSW	13	13,760,748 (GRCm38)	missense	probably damaging	1.00
R9402:Lyst	UTSW	13	13,637,878 (GRCm38)	missense	probably benign
R9457:Lyst	UTSW	13	13,687,745 (GRCm38)	missense	possibly damaging	0.83
R9481:Lyst	UTSW	13	13,683,068 (GRCm38)	missense	possibly damaging	0.68
R9563:Lyst	UTSW	13	13,637,823 (GRCm38)	missense	probably benign	0.36
R9623:Lyst	UTSW	13	13,678,002 (GRCm38)	missense	probably benign
R9661:Lyst	UTSW	13	13,634,194 (GRCm38)	missense	probably benign	0.01
R9682:Lyst	UTSW	13	13,656,941 (GRCm38)	missense	probably benign	0.21
R9743:Lyst	UTSW	13	13,634,738 (GRCm38)	missense	possibly damaging	0.67
R9801:Lyst	UTSW	13	13,634,705 (GRCm38)	missense	probably damaging	0.97
RF001:Lyst	UTSW	13	13,635,841 (GRCm38)	missense	probably benign
RF002:Lyst	UTSW	13	13,634,363 (GRCm38)	missense	probably benign	0.05
X0024:Lyst	UTSW	13	13,634,448 (GRCm38)	missense	probably benign	0.00
X0026:Lyst	UTSW	13	13,751,970 (GRCm38)	missense	probably damaging	0.99
Z1088:Lyst	UTSW	13	13,743,433 (GRCm38)	missense	probably benign	0.09
Z1176:Lyst	UTSW	13	13,777,079 (GRCm38)	missense	probably benign	0.27
Z1176:Lyst	UTSW	13	13,640,107 (GRCm38)	missense	probably damaging	1.00
Z1177:Lyst	UTSW	13	13,680,134 (GRCm38)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- GGCGACAGCGTAAAAGTACC -3'
(R):5'- CTACACTGAGTGCTAGGGAAG -3'

Sequencing Primer
(F):5'- CCCATCGTTATTCTGTAAGAGATGC -3'
(R):5'- TGAGTGCTAGGGAAGAATTATGATTC -3'

Posted On

2016-06-06