Mutagenetix > Incidental Mutation

Incidental Mutation 'R5031:Tram1l1'

391680

Institutional Source

Beutler Lab

Gene Symbol

Tram1l1

Ensembl Gene

ENSMUSG00000044528

Gene Name

translocation associated membrane protein 1-like 1

Synonyms

A830091N21Rik

MMRRC Submission

042622-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R5031 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

124114686-124116909 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 124115293 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 151 (L151*)

Ref Sequence

ENSEMBL: ENSMUSP00000062635 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058994]

AlphaFold

Q8QZR0

Predicted Effect

probably null
Transcript: ENSMUST00000058994
AA Change: L151*

SMART Domains

Protein: ENSMUSP00000062635
Gene: ENSMUSG00000044528
AA Change: L151*

Domain	Start	End	E-Value	Type
Pfam:TRAM1	47	114	4.2e-21	PFAM
TLC	116	324	1.73e-47	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196150

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 95.7%
20x: 89.9%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,247,678 (GRCm39)	N2475I	probably damaging	Het
Acnat2	T	C	4: 49,380,631 (GRCm39)	K231R	probably damaging	Het
Ank1	C	T	8: 23,589,696 (GRCm39)	P599L	probably damaging	Het
Arhgef19	A	G	4: 140,978,121 (GRCm39)	E580G	probably damaging	Het
Atr	A	G	9: 95,747,755 (GRCm39)	K346E	probably damaging	Het
AU021092	T	C	16: 5,030,468 (GRCm39)	K309E	probably damaging	Het
Baz2b	T	C	2: 59,743,151 (GRCm39)	R1607G	probably benign	Het
Cct8	C	T	16: 87,284,426 (GRCm39)	V254M	probably damaging	Het
Cdca2	T	A	14: 67,950,602 (GRCm39)	I110F	probably damaging	Het
Csmd3	A	T	15: 47,522,588 (GRCm39)	C2694S	probably damaging	Het
Dmkn	A	G	7: 30,463,661 (GRCm39)	I105V	probably benign	Het
Dock1	A	G	7: 134,753,975 (GRCm39)	D1584G	probably benign	Het
Epg5	G	A	18: 78,072,163 (GRCm39)	V2392I	probably benign	Het
Gm4787	G	C	12: 81,424,604 (GRCm39)	T518S	probably benign	Het
Gsap	G	A	5: 21,447,824 (GRCm39)	S294N	possibly damaging	Het
Hectd2	A	G	19: 36,577,004 (GRCm39)	N142D	probably damaging	Het
Hmcn1	A	G	1: 150,464,008 (GRCm39)	C5091R	probably damaging	Het
Ifitm5	G	A	7: 140,530,017 (GRCm39)	R36*	probably null	Het
Ints2	G	A	11: 86,147,026 (GRCm39)	P40L	probably damaging	Het
Irs1	A	T	1: 82,264,688 (GRCm39)	L1176*	probably null	Het
Klhl29	C	T	12: 5,141,334 (GRCm39)	R550Q	probably benign	Het
Kyat1	A	G	2: 30,078,102 (GRCm39)	M134T	probably damaging	Het
Lrrk2	A	T	15: 91,584,822 (GRCm39)	N384Y	possibly damaging	Het
Magel2	T	C	7: 62,029,852 (GRCm39)	S919P	unknown	Het
Mettl16	A	T	11: 74,693,825 (GRCm39)	I279F	probably benign	Het
Mmut	T	C	17: 41,249,718 (GRCm39)	F231S	possibly damaging	Het
Mrgpra1	A	T	7: 46,984,985 (GRCm39)	Y231*	probably null	Het
Mroh2a	GCCC	GC	1: 88,159,979 (GRCm39)		probably null	Het
Mvp	A	C	7: 126,592,788 (GRCm39)	Y374*	probably null	Het
Nabp2	G	A	10: 128,245,497 (GRCm39)		probably benign	Het
Nos1	C	T	5: 118,017,378 (GRCm39)	P247L	probably benign	Het
Or10ag53	C	A	2: 87,082,426 (GRCm39)	F48L	probably benign	Het
Or9g4b	T	A	2: 85,616,062 (GRCm39)	L69*	probably null	Het
Pik3cb	C	T	9: 98,953,461 (GRCm39)	D441N	probably damaging	Het
Qrich1	C	T	9: 108,418,935 (GRCm39)	P464S	possibly damaging	Het
Rab17	A	T	1: 90,887,860 (GRCm39)		probably null	Het
Relch	A	G	1: 105,592,239 (GRCm39)	N136S	probably damaging	Het
Rspo3	A	T	10: 29,382,443 (GRCm39)	L77H	probably damaging	Het
Spn	G	T	7: 126,736,402 (GRCm39)	T35K	probably benign	Het
Sult1d1	T	A	5: 87,707,703 (GRCm39)	Y139F	possibly damaging	Het
Tbc1d32	C	A	10: 55,999,627 (GRCm39)	Q848H	probably damaging	Het
Tcaf3	A	G	6: 42,573,867 (GRCm39)	V115A	probably benign	Het
Trappc12	T	A	12: 28,742,512 (GRCm39)	I682L	possibly damaging	Het
Trav6d-4	A	C	14: 52,991,056 (GRCm39)	T31P	probably damaging	Het
Trpm8	A	G	1: 88,275,910 (GRCm39)	T503A	probably benign	Het
Virma	T	A	4: 11,542,116 (GRCm39)	Y1567*	probably null	Het
Vmn1r228	T	A	17: 20,996,943 (GRCm39)	K192*	probably null	Het
Zfp521	T	A	18: 13,977,330 (GRCm39)	T1028S	possibly damaging	Het
Zfp583	T	A	7: 6,320,397 (GRCm39)	Q205L	probably benign	Het

Other mutations in Tram1l1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01293:Tram1l1	APN	3	124,115,788 (GRCm39)	missense	probably benign	0.00
IGL02385:Tram1l1	APN	3	124,115,707 (GRCm39)	missense	probably benign	0.25
IGL03212:Tram1l1	APN	3	124,115,563 (GRCm39)	missense	possibly damaging	0.89
R1440:Tram1l1	UTSW	3	124,115,580 (GRCm39)	nonsense	probably null
R1929:Tram1l1	UTSW	3	124,115,635 (GRCm39)	missense	probably damaging	1.00
R2007:Tram1l1	UTSW	3	124,115,488 (GRCm39)	missense	possibly damaging	0.89
R4623:Tram1l1	UTSW	3	124,115,509 (GRCm39)	missense	possibly damaging	0.47
R5091:Tram1l1	UTSW	3	124,115,400 (GRCm39)	missense	possibly damaging	0.69
R6142:Tram1l1	UTSW	3	124,115,092 (GRCm39)	missense	probably damaging	1.00
R7469:Tram1l1	UTSW	3	124,114,889 (GRCm39)	missense	probably benign	0.02
R7565:Tram1l1	UTSW	3	124,115,556 (GRCm39)	missense	probably damaging	0.99
R7686:Tram1l1	UTSW	3	124,114,959 (GRCm39)	missense	probably benign	0.07
R7841:Tram1l1	UTSW	3	124,115,354 (GRCm39)	missense	probably damaging	1.00
R7841:Tram1l1	UTSW	3	124,115,353 (GRCm39)	missense	probably damaging	1.00
R8139:Tram1l1	UTSW	3	124,115,436 (GRCm39)	missense	probably benign	0.10
R8174:Tram1l1	UTSW	3	124,115,911 (GRCm39)	missense	probably benign	0.00
R9194:Tram1l1	UTSW	3	124,115,137 (GRCm39)	missense	possibly damaging	0.60
R9360:Tram1l1	UTSW	3	124,115,899 (GRCm39)	missense	probably damaging	1.00
X0062:Tram1l1	UTSW	3	124,115,743 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TACATGCTGGTGGCCATCATC -3'
(R):5'- CCCAAGTGGTTCAAGTACAAGAG -3'

Sequencing Primer
(F):5'- GTGGCCATCATCATCCACG -3'
(R):5'- AGAGATAGGCCCCTCCAATGTG -3'

Posted On

2016-06-06