Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL02796:Zfp146'

392025

Institutional Source

Beutler Lab

Gene Symbol

Zfp146

Ensembl Gene

ENSMUSG00000037029

Gene Name

zinc finger protein 146

Synonyms

OZF

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.190) question?

Stock #

IGL02796 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

29860694-29869175 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 29861983 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 20 (V20I)

Ref Sequence

ENSEMBL: ENSMUSP00000058588 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062181]

AlphaFold

Q8BQN6

Predicted Effect

probably benign
Transcript: ENSMUST00000062181
AA Change: V20I

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000058588
Gene: ENSMUSG00000037029
AA Change: V20I

Domain	Start	End	E-Value	Type
ZnF_C2H2	16	38	1.01e-1	SMART
ZnF_C2H2	44	66	5.5e-3	SMART
ZnF_C2H2	72	94	3.44e-4	SMART
ZnF_C2H2	100	122	2.27e-4	SMART
ZnF_C2H2	128	150	1.98e-4	SMART
ZnF_C2H2	156	178	3.89e-3	SMART
ZnF_C2H2	184	206	6.99e-5	SMART
ZnF_C2H2	212	234	1.6e-4	SMART
ZnF_C2H2	240	262	5.5e-3	SMART
ZnF_C2H2	268	290	1.82e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136508

Meta Mutation Damage Score

0.1590

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

98% (50/51)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsl5	A	T	19: 55,266,601 (GRCm39)	K86*	probably null	Het
Ambp	C	A	4: 63,072,169 (GRCm39)		probably benign	Het
Atp6v0d2	A	T	4: 19,887,324 (GRCm39)	M208K	probably damaging	Het
Brinp1	C	G	4: 68,680,427 (GRCm39)	R701P	probably damaging	Het
Btnl9	T	C	11: 49,060,008 (GRCm39)	D580G	probably damaging	Het
Cand1	A	T	10: 119,049,543 (GRCm39)	L401H	probably damaging	Het
Ccdc66	T	C	14: 27,208,523 (GRCm39)	E577G	possibly damaging	Het
Ccdc93	C	A	1: 121,418,895 (GRCm39)	D483E	probably damaging	Het
Cd38	T	C	5: 44,063,555 (GRCm39)	L216P	probably damaging	Het
Cdr1	C	T	X: 60,228,087 (GRCm39)	D360N	possibly damaging	Het
Cibar2	C	T	8: 120,904,138 (GRCm39)		probably benign	Het
Col9a3	G	T	2: 180,255,955 (GRCm39)	D443Y	probably damaging	Het
Cpt1b	C	T	15: 89,309,005 (GRCm39)	V66I	probably benign	Het
Cyp8b1	T	A	9: 121,744,564 (GRCm39)	Y256F	probably benign	Het
Dennd10	A	G	19: 60,806,081 (GRCm39)		probably benign	Het
Dync1i1	A	G	6: 5,757,385 (GRCm39)	N48S	probably benign	Het
Ehd3	T	A	17: 74,123,396 (GRCm39)	L133Q	probably damaging	Het
Eif6	C	A	2: 155,668,068 (GRCm39)	R57L	probably damaging	Het
Fcgbp	T	A	7: 27,800,576 (GRCm39)		probably benign	Het
Fgl1	C	T	8: 41,650,095 (GRCm39)	A229T	probably benign	Het
Flg2	C	T	3: 93,110,920 (GRCm39)	R983W	unknown	Het
Gdf7	A	T	12: 8,351,666 (GRCm39)	S90T	unknown	Het
Ggta1	T	C	2: 35,303,329 (GRCm39)		probably benign	Het
Gm44859	C	T	19: 12,014,112 (GRCm39)		probably benign	Het
Grin2a	T	C	16: 9,402,972 (GRCm39)	E838G	possibly damaging	Het
Grin2c	A	G	11: 115,141,543 (GRCm39)		probably benign	Het
Grm1	T	C	10: 10,565,411 (GRCm39)	T966A	probably benign	Het
Gtpbp1	A	G	15: 79,601,186 (GRCm39)	I468M	possibly damaging	Het
Gucy1b2	G	A	14: 62,645,143 (GRCm39)	P639S	probably benign	Het
Hat1	T	C	2: 71,250,700 (GRCm39)		probably null	Het
Hira	A	T	16: 18,744,404 (GRCm39)	I374F	probably benign	Het
Htt	C	A	5: 35,034,826 (GRCm39)	Q2024K	probably benign	Het
Kcnj6	A	G	16: 94,633,778 (GRCm39)	M111T	probably benign	Het
Loxhd1	T	A	18: 77,456,811 (GRCm39)		probably benign	Het
Lrif1	T	A	3: 106,642,752 (GRCm39)	M619K	probably benign	Het
Lrrc8b	G	A	5: 105,629,211 (GRCm39)	G519D	probably damaging	Het
Map3k7	A	G	4: 31,979,692 (GRCm39)		probably benign	Het
Or2ah1	T	C	2: 85,653,933 (GRCm39)	I206T	probably benign	Het
Or4d6	T	A	19: 12,086,248 (GRCm39)	I221F	possibly damaging	Het
P2ry12	T	C	3: 59,125,302 (GRCm39)	I124M	probably damaging	Het
Paxbp1	A	G	16: 90,822,182 (GRCm39)		probably benign	Het
Polb	A	T	8: 23,121,474 (GRCm39)	I257N	probably damaging	Het
Psg27	A	T	7: 18,295,875 (GRCm39)	V190E	probably benign	Het
Rabgap1	T	A	2: 37,362,318 (GRCm39)	N54K	probably damaging	Het
Sacm1l	T	A	9: 123,377,989 (GRCm39)	W90R	possibly damaging	Het
Snrpb2	A	T	2: 142,906,487 (GRCm39)	M1L	probably benign	Het
Spata31d1d	A	G	13: 59,876,057 (GRCm39)	Y493H	possibly damaging	Het
Speer4c1	A	C	5: 15,919,214 (GRCm39)		probably benign	Het
Spocd1	A	G	4: 129,841,548 (GRCm39)		probably benign	Het
Tbc1d5	G	T	17: 51,273,652 (GRCm39)	H123Q	probably damaging	Het
Ttn	T	C	2: 76,608,445 (GRCm39)	N17826S	probably damaging	Het
Tubgcp6	A	G	15: 89,006,593 (GRCm39)	I143T	probably benign	Het
Usp48	A	G	4: 137,338,029 (GRCm39)	Y268C	probably damaging	Het
Zfhx4	G	A	3: 5,464,599 (GRCm39)	V1586M	probably damaging	Het
Zfp263	G	A	16: 3,564,740 (GRCm39)	E252K	probably benign	Het

Other mutations in Zfp146

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02170:Zfp146	APN	7	29,861,905 (GRCm39)	missense	possibly damaging	0.51
IGL02485:Zfp146	APN	7	29,862,015 (GRCm39)	missense	probably benign
R2043:Zfp146	UTSW	7	29,861,664 (GRCm39)	missense	possibly damaging	0.92
R4166:Zfp146	UTSW	7	29,861,865 (GRCm39)	missense	possibly damaging	0.50
R4385:Zfp146	UTSW	7	29,861,847 (GRCm39)	missense	probably benign
R5270:Zfp146	UTSW	7	29,861,900 (GRCm39)	missense	probably benign	0.03
R5271:Zfp146	UTSW	7	29,861,900 (GRCm39)	missense	probably benign	0.03
R5746:Zfp146	UTSW	7	29,861,818 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CATGCACAATCAGCGACGTG -3'
(R):5'- AGCCCTCGGACTGTAAGAAATG -3'

Sequencing Primer
(F):5'- GCTAAAGGCTTTTCCGCAG -3'
(R):5'- TGTGGAAGCATCCTCAGTCAAG -3'

Posted On

2016-06-08