Mutagenetix > Incidental Mutations

Incidental Mutation 'IGL03052:Mrgprb3'

392180

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb3

Ensembl Gene

ENSMUSG00000070546

Gene Name

MAS-related GPR, member B3

Synonyms

MrgB3

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.095) question?

Stock #

IGL03052 (G1)

Quality Score

152

Status

Validated

Chromosome

Chromosomal Location

48642803-48643811 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 48643593 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 70 (V70E)

Ref Sequence

ENSEMBL: ENSMUSP00000091945 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094383]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094383
AA Change: V70E

PolyPhen 2 Score 0.926 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000091945
Gene: ENSMUSG00000070546
AA Change: V70E

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	25	279	4e-13	SMART
low complexity region	301	312	N/A	INTRINSIC

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930451G09Rik	A	T	16: 4,977,494		noncoding transcript	Het
5830411N06Rik	G	A	7: 140,248,914	C162Y	probably damaging	Het
Acap3	T	C	4: 155,903,358	F517S	probably damaging	Het
Afap1l1	A	G	18: 61,748,823	V267A	probably benign	Het
Asap1	A	G	15: 64,153,834		probably benign	Het
Bcl6	T	C	16: 23,975,038		probably benign	Het
Ccdc73	C	A	2: 104,951,936	H212Q	possibly damaging	Het
Cct5	A	T	15: 31,597,487	H85Q	probably damaging	Het
Cfap69	A	C	5: 5,589,206	L238R	probably damaging	Het
Chl1	A	T	6: 103,691,667	T470S	probably benign	Het
Cnga4	T	C	7: 105,404,725	S12P	probably benign	Het
Cyp2c29	C	T	19: 39,287,218	T34M	possibly damaging	Het
Cyp2c67	T	C	19: 39,648,885	D49G	possibly damaging	Het
Dab2ip	A	G	2: 35,643,897	Q45R	probably benign	Het
Ddhd1	A	G	14: 45,620,783	V164A	probably damaging	Het
Dnah7c	A	G	1: 46,632,149	Y1566C	probably damaging	Het
Dnase1l2	T	C	17: 24,440,994		probably benign	Het
Dock2	G	T	11: 34,232,853	N1593K	probably benign	Het
Dpp6	G	T	5: 27,709,508	M530I	probably benign	Het
Epm2a	T	C	10: 11,457,230	V269A	possibly damaging	Het
Fcna	G	C	2: 25,630,681		probably benign	Het
Fgf2	A	G	3: 37,349,012	S55G	probably benign	Het
Frem3	C	T	8: 80,614,530	P1151S	probably damaging	Het
Gm12666	A	G	4: 92,191,050	L178P	probably benign	Het
Gm15737	T	C	6: 92,869,500		probably benign	Het
Gm1840	T	A	8: 5,639,816		noncoding transcript	Het
Gm4759	T	C	7: 106,423,695		noncoding transcript	Het
Hoxa3	G	A	6: 52,170,287		probably benign	Het
Macf1	T	C	4: 123,387,395	I3770V	probably damaging	Het
Mapk15	G	T	15: 75,993,882	R8L	probably benign	Het
Mecom	C	T	3: 29,960,963		probably benign	Het
Mknk2	C	T	10: 80,669,662	R154H	probably benign	Het
Mtdh	A	G	15: 34,140,730	K570E	possibly damaging	Het
Myo5c	G	T	9: 75,252,516		probably benign	Het
Myom2	T	C	8: 15,123,442		probably benign	Het
Olfr1489	T	C	19: 13,633,726	I205T	probably benign	Het
Olfr553	C	T	7: 102,614,449	R180Q	probably benign	Het
Olfr843	A	T	9: 19,248,642	Y252*	probably null	Het
Pcca	A	T	14: 122,887,101	M695L	probably benign	Het
Pcdha2	A	T	18: 36,941,617	D767V	probably damaging	Het
Plekhs1	G	A	19: 56,470,757	D16N	probably benign	Het
Prr11	T	C	11: 87,103,652	N56S	possibly damaging	Het
Rxfp2	A	T	5: 150,043,180		probably benign	Het
Sacs	G	T	14: 61,207,858	G2451V	probably damaging	Het
Scarb1	G	A	5: 125,294,099	A4V	probably damaging	Het
Sik3	C	A	9: 46,198,149	T475K	probably damaging	Het
Slco1b2	A	T	6: 141,648,585	I59L	probably benign	Het
Sspo	G	A	6: 48,460,453	G1382R	probably damaging	Het
Stx16	C	A	2: 174,092,438	P145T	probably benign	Het
Tnrc18	G	A	5: 142,775,219	A674V	unknown	Het
Uqcrq	A	G	11: 53,430,649	V14A	possibly damaging	Het
Vmn2r79	A	T	7: 87,003,591	E497V	probably benign	Het
Vps8	T	A	16: 21,448,365	I166K	probably damaging	Het
Vwa8	A	G	14: 79,064,921	D1010G	probably benign	Het
Wnk1	T	A	6: 119,944,799		probably benign	Het
Zfand3	T	A	17: 30,060,824	M29K	probably benign	Het

Other mutations in Mrgprb3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03069:Mrgprb3	APN	7	48643450	missense	possibly damaging	0.71
Reserve	UTSW	7	48643699	missense	probably benign	0.02
starker	UTSW	7	48643368	missense	probably benign	0.28
R0446:Mrgprb3	UTSW	7	48643236	missense	probably benign	0.42
R0546:Mrgprb3	UTSW	7	48643515	missense	probably damaging	1.00
R0885:Mrgprb3	UTSW	7	48643096	missense	probably damaging	1.00
R1764:Mrgprb3	UTSW	7	48643023	missense	probably benign	0.01
R2044:Mrgprb3	UTSW	7	48643734	missense	possibly damaging	0.92
R2230:Mrgprb3	UTSW	7	48643022	missense	probably benign	0.05
R2232:Mrgprb3	UTSW	7	48643022	missense	probably benign	0.05
R2240:Mrgprb3	UTSW	7	48643641	missense	probably damaging	0.99
R3001:Mrgprb3	UTSW	7	48643484	missense	probably benign
R3002:Mrgprb3	UTSW	7	48643484	missense	probably benign
R4717:Mrgprb3	UTSW	7	48643252	missense	probably benign	0.01
R4805:Mrgprb3	UTSW	7	48643306	missense	probably benign	0.01
R5083:Mrgprb3	UTSW	7	48643014	missense	probably benign	0.01
R5311:Mrgprb3	UTSW	7	48643311	missense	probably damaging	1.00
R5330:Mrgprb3	UTSW	7	48642934	missense	possibly damaging	0.90
R5331:Mrgprb3	UTSW	7	48642934	missense	possibly damaging	0.90
R5615:Mrgprb3	UTSW	7	48643486	missense	probably benign	0.01
R5621:Mrgprb3	UTSW	7	48643368	missense	probably benign	0.28
R5697:Mrgprb3	UTSW	7	48642925	missense	probably damaging	0.96
R5725:Mrgprb3	UTSW	7	48643800	start codon destroyed	probably null	0.02
R5758:Mrgprb3	UTSW	7	48643319	missense	probably benign	0.01
R5807:Mrgprb3	UTSW	7	48643362	missense	probably benign	0.02
R5908:Mrgprb3	UTSW	7	48643618	missense	probably damaging	0.98
R6902:Mrgprb3	UTSW	7	48643699	missense	probably benign	0.02
R7037:Mrgprb3	UTSW	7	48643194	missense	probably damaging	1.00
R7288:Mrgprb3	UTSW	7	48643311	missense	probably damaging	1.00
R7605:Mrgprb3	UTSW	7	48643114	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ACCAGATGGGCCACATGATAG -3'
(R):5'- AGCATGGCTCTAAGAACCTC -3'

Sequencing Primer
(F):5'- TAGACAGGCAGCACTCAATG -3'
(R):5'- TGGCTCTAAGAACCTCACTAATAAC -3'

Posted On

2016-06-09