Mutagenetix > Incidental Mutation

Incidental Mutation 'R5120:Gm8674'

392957

Institutional Source

Beutler Lab

Gene Symbol

Gm8674

Ensembl Gene

ENSMUSG00000093976

Gene Name

predicted gene 8674

Synonyms

MMRRC Submission

042708-MU

Accession Numbers

Genbank: XM_990959; MGI: 3645762

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R5120 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

49899119-49904597 bp(-) (GRCm38)

Type of Mutation

exon

DNA Base Change (assembly)

C to A at 49901948 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000179367

SMART Domains

Protein: ENSMUSP00000137219
Gene: ENSMUSG00000093976

Domain	Start	End	E-Value	Type
low complexity region	70	86	N/A	INTRINSIC
Pfam:FAM75	92	444	3.6e-27	PFAM

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 95.8%
20x: 90.6%

Validation Efficiency

100% (97/97)

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310033P09Rik	A	G	11: 59,210,235	H225R	probably benign	Het
Abcc9	T	A	6: 142,656,618	I690F	probably benign	Het
Acp4	A	T	7: 44,256,971	D52E	probably damaging	Het
Adam17	T	C	12: 21,343,019		probably benign	Het
Adamts15	T	C	9: 30,921,576	E221G	probably damaging	Het
Aoc2	A	G	11: 101,325,714	T208A	probably benign	Het
Apoa4	T	G	9: 46,242,737	I212S	probably damaging	Het
Bptf	A	T	11: 107,073,385	M1598K	probably damaging	Het
Camsap3	G	A	8: 3,600,680	R209Q	probably damaging	Het
Ccdc171	C	A	4: 83,558,526		probably benign	Het
Celsr2	G	C	3: 108,393,120	P2875A	probably benign	Het
Cenpb	A	G	2: 131,179,818	V20A	probably benign	Het
Cfap61	A	T	2: 146,143,160	K975*	probably null	Het
Col9a2	G	T	4: 121,039,772	A20S	unknown	Het
Crnn	A	T	3: 93,148,896	I330F	probably benign	Het
Csf3r	C	A	4: 126,035,827	P381Q	probably benign	Het
Csmd3	G	A	15: 48,673,495	Q104*	probably null	Het
Ctnna1	G	A	18: 35,182,554		probably null	Het
Cwf19l2	C	T	9: 3,418,761	Q183*	probably null	Het
Ddx60	T	C	8: 61,945,906	Y220H	probably benign	Het
Dhh	T	C	15: 98,898,157	Q39R	probably benign	Het
Dynlrb2	T	A	8: 116,515,698	I89N	possibly damaging	Het
Dytn	T	C	1: 63,623,043	E645G	probably benign	Het
Efcab7	A	G	4: 99,897,491	T287A	probably damaging	Het
Eogt	A	G	6: 97,134,315	V195A	probably benign	Het
Ep400	G	T	5: 110,756,358	P125Q	probably damaging	Het
Fasn	A	G	11: 120,811,391	V1815A	probably benign	Het
Gm4788	T	C	1: 139,753,103	S226G	probably benign	Het
Gm5866	T	C	5: 52,582,882		noncoding transcript	Het
Gpat4	G	T	8: 23,180,202	H270Q	possibly damaging	Het
Grik5	A	G	7: 25,010,640	L890P	probably damaging	Het
Gsap	A	G	5: 21,269,936	N500S	probably damaging	Het
H2-M10.1	T	C	17: 36,325,156	D173G	probably benign	Het
Igfn1	T	A	1: 135,973,502	I413F	possibly damaging	Het
Ighv1-52	G	T	12: 115,145,786	H17N	probably benign	Het
Kcnu1	T	A	8: 25,934,488	D270E	possibly damaging	Het
Kif16b	A	C	2: 142,848,339	N274K	probably damaging	Het
Klk1b11	C	T	7: 43,999,022	T151I	probably benign	Het
Krtap13	A	G	16: 88,751,570	F10S	probably damaging	Het
Large1	A	T	8: 72,859,341	I379N	probably damaging	Het
Lhx8	T	C	3: 154,311,695	H301R	probably damaging	Het
Lrriq3	T	C	3: 155,129,384	V252A	probably benign	Het
Lysmd3	T	A	13: 81,669,192	I96N	probably damaging	Het
Mpeg1	C	T	19: 12,461,429	Q84*	probably null	Het
Myoz1	C	T	14: 20,650,654	G165D	probably benign	Het
Myt1	G	A	2: 181,797,620	V312I	probably benign	Het
Ndufb7	T	C	8: 83,566,977		probably benign	Het
Numa1	C	T	7: 101,977,437	T10M	probably damaging	Het
Olfr1285	T	C	2: 111,409,240		noncoding transcript	Het
Olfr130	A	T	17: 38,067,266	I32F	probably damaging	Het
Olfr1316	A	T	2: 112,130,558	D84E	probably damaging	Het
Olfr149	T	A	9: 39,702,070	H233L	probably benign	Het
Olfr390	A	C	11: 73,786,964	I9L	probably benign	Het
Osmr	A	T	15: 6,827,275	S464T	probably benign	Het
Pak7	G	A	2: 136,083,229	H718Y	probably damaging	Het
Pan2	T	A	10: 128,314,995		probably null	Het
Pik3r4	T	C	9: 105,669,009	S853P	probably benign	Het
Pnkp	C	T	7: 44,862,403	S113L	probably damaging	Het
Polr3gl	T	A	3: 96,578,479		probably benign	Het
Psd2	G	A	18: 35,979,810	R186Q	possibly damaging	Het
Psd4	T	C	2: 24,405,438	V868A	probably benign	Het
Ralgapa2	G	T	2: 146,412,084	T852K	probably benign	Het
Rbm43	T	C	2: 51,932,423		probably benign	Het
Rps6kl1	C	A	12: 85,139,348	G329C	probably damaging	Het
Scaf11	T	C	15: 96,419,542	T714A	probably benign	Het
Selenoo	C	A	15: 89,094,305	N310K	possibly damaging	Het
Sepsecs	T	C	5: 52,660,661	Q258R	probably damaging	Het
Sgcz	T	C	8: 37,526,266	S226G	probably benign	Het
Slfn5	A	G	11: 82,960,928	K627E	probably damaging	Het
Sos1	A	T	17: 80,408,248	F1027I	probably damaging	Het
Ssh1	C	G	5: 113,957,398	V228L	possibly damaging	Het
Sugp1	C	A	8: 70,048,667	P65T	probably benign	Het
Tas2r106	T	A	6: 131,678,816	N24I	probably damaging	Het
Tex10	T	C	4: 48,459,272	E534G	possibly damaging	Het
Tmem182	C	T	1: 40,854,901	T192I	possibly damaging	Het
Traf7	T	A	17: 24,518,744	K51*	probably null	Het
Trap1	C	A	16: 4,044,088	R604L	probably damaging	Het
Trim6	T	A	7: 104,228,240	L179Q	probably damaging	Het
Ubfd1	T	C	7: 122,071,750	S264P	probably damaging	Het
Upk3bl	T	C	5: 136,064,191		probably benign	Het
Wdfy3	T	A	5: 101,868,106	Q2601L	possibly damaging	Het
Wdr89	A	G	12: 75,632,638	Y281H	probably damaging	Het
Zfp286	A	T	11: 62,780,725	M174K	probably benign	Het
Zmym1	T	C	4: 127,051,437		probably null	Het

Other mutations in Gm8674

Allele	Source	Chr	Coord	Type	Predicted Effect
R0355:Gm8674	UTSW	13	49901939	exon	noncoding transcript
R0357:Gm8674	UTSW	13	49902113	exon	noncoding transcript
R0627:Gm8674	UTSW	13	49899715	exon	noncoding transcript
R0833:Gm8674	UTSW	13	49904575	exon	noncoding transcript
R1300:Gm8674	UTSW	13	49901722	exon	noncoding transcript
R1452:Gm8674	UTSW	13	49900517	exon	noncoding transcript
R1542:Gm8674	UTSW	13	49900003	exon	noncoding transcript
R1613:Gm8674	UTSW	13	49902438	intron	noncoding transcript
R1643:Gm8674	UTSW	13	49901358	exon	noncoding transcript
R1732:Gm8674	UTSW	13	49901926	exon	noncoding transcript
R1824:Gm8674	UTSW	13	49900808	exon	noncoding transcript
R1840:Gm8674	UTSW	13	49901765	exon	noncoding transcript
R1915:Gm8674	UTSW	13	49900853	exon	noncoding transcript
R1934:Gm8674	UTSW	13	49901435	exon	noncoding transcript
R2040:Gm8674	UTSW	13	49901669	exon	noncoding transcript
R2214:Gm8674	UTSW	13	49901360	exon	noncoding transcript
R2421:Gm8674	UTSW	13	49900663	exon	noncoding transcript
R3423:Gm8674	UTSW	13	49901756	exon	noncoding transcript
R3425:Gm8674	UTSW	13	49901756	exon	noncoding transcript
R3886:Gm8674	UTSW	13	49902163	splice site	noncoding transcript
R4083:Gm8674	UTSW	13	49901011	exon	noncoding transcript
R4343:Gm8674	UTSW	13	49899706	exon	noncoding transcript
R4570:Gm8674	UTSW	13	49902534	intron	noncoding transcript
R4936:Gm8674	UTSW	13	49900755	exon	noncoding transcript
R4967:Gm8674	UTSW	13	49901998	exon	noncoding transcript
R5065:Gm8674	UTSW	13	49902577	intron	noncoding transcript
R5067:Gm8674	UTSW	13	49899834	exon	noncoding transcript
R5208:Gm8674	UTSW	13	49901921	exon	noncoding transcript
R5268:Gm8674	UTSW	13	49901354	exon	noncoding transcript
R5471:Gm8674	UTSW	13	49900813	exon	noncoding transcript
R5773:Gm8674	UTSW	13	49901876	exon	noncoding transcript
R5809:Gm8674	UTSW	13	49901888	exon	noncoding transcript
R8162:Gm8674	UTSW	13	49900371	missense	noncoding transcript
R8239:Gm8674	UTSW	13	49900226	missense	noncoding transcript
Z1088:Gm8674	UTSW	13	49900794	exon	noncoding transcript
Z1088:Gm8674	UTSW	13	49901248	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- TTCCAATAAAGTCCATGTGGCC -3'
(R):5'- TGGAGGGTCTCATACACAAGG -3'

Sequencing Primer
(F):5'- CATGTGGCCATGGCTGG -3'
(R):5'- AGTCAAATTTCCTGGGGCAC -3'

Posted On

2016-06-15