Mutagenetix > Incidental Mutation

Incidental Mutation 'R5121:Golgb1'

393070

Institutional Source

Beutler Lab

Gene Symbol

Golgb1

Ensembl Gene

ENSMUSG00000034243

Gene Name

golgi autoantigen, golgin subfamily b, macrogolgin 1

Synonyms

Giantin, C130074L01Rik, F730017E11Rik, Gm6840, 6330407A06Rik

MMRRC Submission

042709-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.911) question?

Stock #

R5121 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36875140-36933085 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 36919258 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 2653 (V2653A)

Ref Sequence

ENSEMBL: ENSMUSP00000110460 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039855] [ENSMUST00000114812]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000039855
AA Change: V2694A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000045239
Gene: ENSMUSG00000034243
AA Change: V2694A

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	7.47e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
coiled coil region	130	219	N/A	INTRINSIC
low complexity region	491	512	N/A	INTRINSIC
internal_repeat_3	519	558	7.47e-6	PROSPERO
coiled coil region	563	594	N/A	INTRINSIC
internal_repeat_4	627	661	3.38e-5	PROSPERO
coiled coil region	679	1121	N/A	INTRINSIC
coiled coil region	1153	1240	N/A	INTRINSIC
internal_repeat_4	1253	1288	3.38e-5	PROSPERO
low complexity region	1300	1314	N/A	INTRINSIC
internal_repeat_1	1321	1352	3.51e-6	PROSPERO
low complexity region	1357	1369	N/A	INTRINSIC
coiled coil region	1402	1755	N/A	INTRINSIC
internal_repeat_2	1760	1798	7.47e-6	PROSPERO
internal_repeat_3	1761	1804	7.47e-6	PROSPERO
coiled coil region	1818	2034	N/A	INTRINSIC
low complexity region	2291	2306	N/A	INTRINSIC
internal_repeat_1	2351	2382	3.51e-6	PROSPERO
low complexity region	2400	2418	N/A	INTRINSIC
low complexity region	2538	2549	N/A	INTRINSIC
coiled coil region	2775	2827	N/A	INTRINSIC
coiled coil region	2854	2943	N/A	INTRINSIC
low complexity region	2964	2976	N/A	INTRINSIC
coiled coil region	3007	3057	N/A	INTRINSIC
coiled coil region	3117	3163	N/A	INTRINSIC
transmembrane domain	3215	3237	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114812
AA Change: V2653A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000110460
Gene: ENSMUSG00000034243
AA Change: V2653A

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	6.71e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
low complexity region	120	134	N/A	INTRINSIC
low complexity region	143	154	N/A	INTRINSIC
low complexity region	200	219	N/A	INTRINSIC
low complexity region	450	471	N/A	INTRINSIC
internal_repeat_3	478	517	6.71e-6	PROSPERO
coiled coil region	522	553	N/A	INTRINSIC
internal_repeat_4	586	620	3.05e-5	PROSPERO
coiled coil region	638	1080	N/A	INTRINSIC
coiled coil region	1112	1199	N/A	INTRINSIC
internal_repeat_4	1212	1247	3.05e-5	PROSPERO
low complexity region	1259	1273	N/A	INTRINSIC
internal_repeat_1	1280	1311	3.14e-6	PROSPERO
low complexity region	1316	1328	N/A	INTRINSIC
coiled coil region	1361	1714	N/A	INTRINSIC
internal_repeat_2	1719	1757	6.71e-6	PROSPERO
internal_repeat_3	1720	1763	6.71e-6	PROSPERO
coiled coil region	1777	1993	N/A	INTRINSIC
low complexity region	2250	2265	N/A	INTRINSIC
internal_repeat_1	2310	2341	3.14e-6	PROSPERO
low complexity region	2359	2377	N/A	INTRINSIC
low complexity region	2497	2508	N/A	INTRINSIC
coiled coil region	2734	2786	N/A	INTRINSIC
coiled coil region	2813	2902	N/A	INTRINSIC
low complexity region	2923	2935	N/A	INTRINSIC
coiled coil region	2966	3016	N/A	INTRINSIC
coiled coil region	3076	3122	N/A	INTRINSIC
transmembrane domain	3174	3196	N/A	INTRINSIC

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.1%
20x: 91.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous knockout affects glycosylation of glycoproteins in the extra-cellular matrix of the palatal shelves, resulting in their failure to elevate and fuse, leading to cleft palate. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted, other(2) Gene trapped(5)

Other mutations in this stock

Total: 101 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acvr1c	A	G	2: 58,281,650 (GRCm38)	I234T	probably damaging	Het
Adam24	A	G	8: 40,679,511 (GRCm38)	E6G	probably damaging	Het
Amz1	A	G	5: 140,744,164 (GRCm38)	D151G	probably benign	Het
Arhgap24	T	C	5: 102,841,335 (GRCm38)	L10P	probably damaging	Het
Arhgap25	T	G	6: 87,532,864 (GRCm38)	N8T	probably benign	Het
Arsi	T	G	18: 60,917,439 (GRCm38)	F465V	probably damaging	Het
Atp2c1	A	G	9: 105,448,825 (GRCm38)	V293A	probably damaging	Het
Bzw2	A	G	12: 36,104,351 (GRCm38)	L340P	probably damaging	Het
Camkmt	C	T	17: 85,096,581 (GRCm38)	T77I	probably benign	Het
Camsap1	G	T	2: 25,935,550 (GRCm38)	Q1375K	probably benign	Het
Catsper2	G	T	2: 121,397,123 (GRCm38)		probably null	Het
Cd163	T	C	6: 124,317,989 (GRCm38)	C671R	probably damaging	Het
Cdk7	T	C	13: 100,717,684 (GRCm38)		probably null	Het
Cfi	C	T	3: 129,873,077 (GRCm38)	P483L	probably damaging	Het
Chn2	C	T	6: 54,218,561 (GRCm38)	L72F	possibly damaging	Het
Cntn2	C	A	1: 132,517,060 (GRCm38)	E363*	probably null	Het
Crebbp	T	C	16: 4,093,511 (GRCm38)	E999G	probably damaging	Het
Cspp1	T	A	1: 10,126,463 (GRCm38)	N900K	probably damaging	Het
Cts3	A	C	13: 61,567,595 (GRCm38)	I141M	probably benign	Het
Cwc27	A	T	13: 104,804,353 (GRCm38)	V166D	probably damaging	Het
Defb26	T	C	2: 152,508,165 (GRCm38)	E65G	possibly damaging	Het
Dnah8	A	G	17: 30,810,353 (GRCm38)	T4099A	probably benign	Het
Eml6	A	G	11: 29,744,606 (GRCm38)	F1953L	probably benign	Het
Etl4	T	A	2: 20,340,111 (GRCm38)		probably null	Het
Fbln1	A	G	15: 85,237,671 (GRCm38)	E331G	probably damaging	Het
Gabra4	G	A	5: 71,572,203 (GRCm38)	H76Y	probably benign	Het
Gcat	T	C	15: 79,035,282 (GRCm38)	V149A	probably damaging	Het
Glp2r	A	T	11: 67,722,100 (GRCm38)		probably null	Het
Gm572	T	C	4: 148,666,845 (GRCm38)		probably null	Het
Grap2	G	A	15: 80,646,144 (GRCm38)	R155Q	possibly damaging	Het
Gspt1	T	A	16: 11,223,301 (GRCm38)	I533L	probably damaging	Het
Gypa	A	T	8: 80,496,348 (GRCm38)	Y27F	unknown	Het
Hipk4	G	A	7: 27,529,492 (GRCm38)	V456I	probably benign	Het
Homer2	A	T	7: 81,649,563 (GRCm38)	D51E	probably benign	Het
Hspa1b	A	T	17: 34,958,004 (GRCm38)	V335E	possibly damaging	Het
Ift122	A	G	6: 115,912,534 (GRCm38)	T827A	probably benign	Het
Igkv1-35	T	C	6: 70,011,135 (GRCm38)	N58S	probably benign	Het
Kat6b	A	T	14: 21,619,258 (GRCm38)	H297L	probably damaging	Het
Kirrel3	G	T	9: 35,013,305 (GRCm38)	G296W	probably damaging	Het
Klhl17	A	G	4: 156,230,625 (GRCm38)	V525A	probably benign	Het
Lrrn1	G	T	6: 107,569,207 (GRCm38)	R655S	possibly damaging	Het
Ltk	T	C	2: 119,753,227 (GRCm38)	N256D	probably damaging	Het
Mill2	G	A	7: 18,856,666 (GRCm38)	G209S	probably benign	Het
Mmp27	A	T	9: 7,581,368 (GRCm38)	H544L	probably benign	Het
Mphosph10	A	G	7: 64,389,596 (GRCm38)	S209P	probably damaging	Het
Mphosph8	A	T	14: 56,676,546 (GRCm38)	K415*	probably null	Het
Myb	T	A	10: 21,126,238 (GRCm38)	M616L	probably benign	Het
Myo15b	C	T	11: 115,886,054 (GRCm38)	R867W	probably damaging	Het
Myo18b	A	T	5: 112,874,480 (GRCm38)		probably benign	Het
Nip7	A	G	8: 107,056,957 (GRCm38)	E8G	possibly damaging	Het
Olfr1318	A	T	2: 112,156,286 (GRCm38)	M112L	possibly damaging	Het
Olfr368	T	G	2: 37,332,589 (GRCm38)	F281V	probably damaging	Het
Olfr38	T	A	6: 42,762,997 (GRCm38)	L315*	probably null	Het
Olfr677	A	G	7: 105,056,482 (GRCm38)	T79A	possibly damaging	Het
Olfr711	T	C	7: 106,972,231 (GRCm38)	I38V	probably benign	Het
Optn	T	A	2: 5,046,106 (GRCm38)	I155F	probably benign	Het
Papolb	A	T	5: 142,528,837 (GRCm38)	H350Q	probably benign	Het
Pappa2	T	A	1: 158,838,627 (GRCm38)	M1128L	probably benign	Het
Pcdhb5	T	A	18: 37,321,117 (GRCm38)	N183K	probably benign	Het
Pde12	G	T	14: 26,669,422 (GRCm38)	S44*	probably null	Het
Peg3	T	C	7: 6,710,289 (GRCm38)	K645E	probably benign	Het
Pkd1	C	T	17: 24,573,463 (GRCm38)	R1375C	probably benign	Het
Plekha5	G	A	6: 140,579,474 (GRCm38)	E21K	probably damaging	Het
Plk2	C	T	13: 110,399,424 (GRCm38)	P554L	probably benign	Het
Pnkp	C	T	7: 44,862,403 (GRCm38)	S113L	probably damaging	Het
Polg	A	T	7: 79,464,605 (GRCm38)	W203R	probably damaging	Het
Ppcdc	A	G	9: 57,421,163 (GRCm38)	V65A	possibly damaging	Het
Ppm1m	T	A	9: 106,195,805 (GRCm38)	Q353L	probably benign	Het
Ppp1r13l	G	C	7: 19,370,095 (GRCm38)	R167P	probably damaging	Het
Ppp1r9b	T	A	11: 94,996,653 (GRCm38)	V497E	probably damaging	Het
Prpf6	T	A	2: 181,636,043 (GRCm38)	H399Q	probably benign	Het
Psg25	T	A	7: 18,526,536 (GRCm38)	I146F	possibly damaging	Het
Rasgef1c	A	T	11: 49,960,429 (GRCm38)	Q116L	probably damaging	Het
Rbbp9	A	G	2: 144,550,756 (GRCm38)	V8A	possibly damaging	Het
Rfk	T	C	19: 17,399,566 (GRCm38)	F144S	probably damaging	Het
Rfwd3	A	C	8: 111,282,753 (GRCm38)		probably null	Het
Rhpn1	T	C	15: 75,709,260 (GRCm38)	I117T	probably damaging	Het
Sars2	A	T	7: 28,747,908 (GRCm38)	N244Y	probably damaging	Het
Scnn1b	A	T	7: 121,902,887 (GRCm38)	H256L	probably benign	Het
Sgcz	G	A	8: 37,539,667 (GRCm38)	T195I	probably damaging	Het
Sh3gl2	T	A	4: 85,379,257 (GRCm38)	H157Q	probably benign	Het
Spen	T	A	4: 141,476,099 (GRCm38)	Q1739L	probably benign	Het
Susd1	G	T	4: 59,379,657 (GRCm38)	S323R	possibly damaging	Het
Szt2	T	C	4: 118,385,444 (GRCm38)	E1482G	possibly damaging	Het
Tex15	A	T	8: 33,571,766 (GRCm38)	K408I	probably damaging	Het
Tex33	C	T	15: 78,386,173 (GRCm38)	E132K	probably benign	Het
Trem1	T	A	17: 48,232,836 (GRCm38)	F14L	probably null	Het
Trim26	G	T	17: 36,851,066 (GRCm38)	E126*	probably null	Het
Trpv3	T	C	11: 73,277,834 (GRCm38)		probably null	Het
Ttn	T	A	2: 76,916,491 (GRCm38)		probably null	Het
Ubap1	C	T	4: 41,379,688 (GRCm38)	L301F	probably benign	Het
Uchl1	T	C	5: 66,676,437 (GRCm38)	M12T	probably benign	Het
Vill	C	T	9: 119,070,025 (GRCm38)	T253I	possibly damaging	Het
Vmn2r107	C	T	17: 20,355,753 (GRCm38)	T115I	probably benign	Het
Vmn2r98	T	C	17: 19,053,553 (GRCm38)	S21P	probably benign	Het
Wapl	A	G	14: 34,677,162 (GRCm38)	K63E	probably benign	Het
Wnt2	T	C	6: 18,023,126 (GRCm38)	K175E	possibly damaging	Het
Zfp592	T	C	7: 81,023,561 (GRCm38)	L91P	probably damaging	Het
Zfp74	T	C	7: 29,932,507 (GRCm38)		probably null	Het
Zglp1	A	T	9: 21,062,661 (GRCm38)	I243N	probably benign	Het
Zranb1	A	G	7: 132,950,187 (GRCm38)	E215G	probably benign	Het

Other mutations in Golgb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01394:Golgb1	APN	16	36,931,564 (GRCm38)	missense	probably damaging	1.00
IGL01717:Golgb1	APN	16	36,915,502 (GRCm38)	nonsense	probably null
IGL01965:Golgb1	APN	16	36,917,920 (GRCm38)	missense	probably damaging	1.00
IGL02128:Golgb1	APN	16	36,916,304 (GRCm38)	missense	probably damaging	1.00
IGL02268:Golgb1	APN	16	36,913,128 (GRCm38)	missense	probably benign	0.25
IGL02383:Golgb1	APN	16	36,886,200 (GRCm38)	missense	probably benign	0.01
IGL02444:Golgb1	APN	16	36,907,816 (GRCm38)	splice site	probably benign
IGL02635:Golgb1	APN	16	36,915,013 (GRCm38)	missense	probably benign	0.00
IGL02655:Golgb1	APN	16	36,918,080 (GRCm38)	missense	probably damaging	0.98
IGL02887:Golgb1	APN	16	36,925,849 (GRCm38)	missense	probably damaging	0.99
IGL02937:Golgb1	APN	16	36,916,210 (GRCm38)	missense	probably damaging	1.00
IGL02973:Golgb1	APN	16	36,912,080 (GRCm38)	missense	possibly damaging	0.92
IGL02982:Golgb1	APN	16	36,925,810 (GRCm38)	missense	probably damaging	0.98
IGL03065:Golgb1	APN	16	36,912,866 (GRCm38)	missense	probably benign	0.11
IGL03109:Golgb1	APN	16	36,915,611 (GRCm38)	missense	possibly damaging	0.93
IGL03323:Golgb1	APN	16	36,913,453 (GRCm38)	nonsense	probably null
I2288:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
I2289:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0080:Golgb1	UTSW	16	36,898,611 (GRCm38)	missense	probably damaging	1.00
R0102:Golgb1	UTSW	16	36,875,468 (GRCm38)	intron	probably benign
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0276:Golgb1	UTSW	16	36,913,876 (GRCm38)	missense	probably damaging	1.00
R0394:Golgb1	UTSW	16	36,875,579 (GRCm38)	intron	probably benign
R0469:Golgb1	UTSW	16	36,931,635 (GRCm38)	missense	probably benign	0.41
R0522:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R0575:Golgb1	UTSW	16	36,918,809 (GRCm38)	missense	probably benign
R0600:Golgb1	UTSW	16	36,916,271 (GRCm38)	missense	probably damaging	1.00
R0608:Golgb1	UTSW	16	36,916,330 (GRCm38)	nonsense	probably null
R0711:Golgb1	UTSW	16	36,918,790 (GRCm38)	missense	probably damaging	1.00
R0785:Golgb1	UTSW	16	36,898,790 (GRCm38)	missense	possibly damaging	0.95
R0893:Golgb1	UTSW	16	36,912,277 (GRCm38)	missense	possibly damaging	0.64
R1163:Golgb1	UTSW	16	36,916,126 (GRCm38)	missense	possibly damaging	0.50
R1208:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R1315:Golgb1	UTSW	16	36,914,900 (GRCm38)	missense	probably benign	0.40
R1429:Golgb1	UTSW	16	36,900,563 (GRCm38)	missense	possibly damaging	0.93
R1505:Golgb1	UTSW	16	36,919,643 (GRCm38)	missense	possibly damaging	0.79
R1537:Golgb1	UTSW	16	36,898,788 (GRCm38)	missense	possibly damaging	0.89
R1610:Golgb1	UTSW	16	36,926,101 (GRCm38)	missense	probably benign	0.25
R1659:Golgb1	UTSW	16	36,887,617 (GRCm38)	missense	probably benign	0.01
R1769:Golgb1	UTSW	16	36,916,001 (GRCm38)	missense	probably damaging	1.00
R2105:Golgb1	UTSW	16	36,914,664 (GRCm38)	missense	probably benign
R2212:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R2261:Golgb1	UTSW	16	36,893,360 (GRCm38)	missense	probably damaging	1.00
R2352:Golgb1	UTSW	16	36,898,559 (GRCm38)	missense	probably damaging	0.99
R2357:Golgb1	UTSW	16	36,912,008 (GRCm38)	missense	probably damaging	1.00
R2400:Golgb1	UTSW	16	36,918,466 (GRCm38)	missense	possibly damaging	0.62
R2513:Golgb1	UTSW	16	36,915,151 (GRCm38)	missense	possibly damaging	0.73
R3103:Golgb1	UTSW	16	36,894,849 (GRCm38)	missense	probably damaging	1.00
R3413:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R3748:Golgb1	UTSW	16	36,918,912 (GRCm38)	missense	probably benign	0.00
R3847:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3850:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3936:Golgb1	UTSW	16	36,914,056 (GRCm38)	nonsense	probably null
R3975:Golgb1	UTSW	16	36,918,571 (GRCm38)	missense	probably damaging	0.99
R4025:Golgb1	UTSW	16	36,915,344 (GRCm38)	missense	probably benign	0.00
R4369:Golgb1	UTSW	16	36,916,907 (GRCm38)	missense	probably damaging	1.00
R4518:Golgb1	UTSW	16	36,929,263 (GRCm38)	missense	probably damaging	0.98
R4600:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4610:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4660:Golgb1	UTSW	16	36,887,618 (GRCm38)	missense	probably damaging	0.99
R4811:Golgb1	UTSW	16	36,891,419 (GRCm38)	missense	probably damaging	1.00
R4815:Golgb1	UTSW	16	36,913,115 (GRCm38)	missense	possibly damaging	0.79
R4835:Golgb1	UTSW	16	36,891,407 (GRCm38)	missense	possibly damaging	0.86
R4904:Golgb1	UTSW	16	36,893,386 (GRCm38)	missense	probably damaging	1.00
R4916:Golgb1	UTSW	16	36,916,118 (GRCm38)	missense	probably benign	0.05
R5133:Golgb1	UTSW	16	36,891,457 (GRCm38)	missense	possibly damaging	0.75
R5143:Golgb1	UTSW	16	36,898,689 (GRCm38)	missense	probably benign	0.09
R5185:Golgb1	UTSW	16	36,875,141 (GRCm38)	unclassified	probably benign
R5188:Golgb1	UTSW	16	36,918,465 (GRCm38)	missense	probably benign	0.13
R5260:Golgb1	UTSW	16	36,913,141 (GRCm38)	missense	probably benign	0.00
R5297:Golgb1	UTSW	16	36,875,616 (GRCm38)	intron	probably benign
R5386:Golgb1	UTSW	16	36,912,315 (GRCm38)	nonsense	probably null
R5438:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5439:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5494:Golgb1	UTSW	16	36,928,683 (GRCm38)	missense	possibly damaging	0.67
R5592:Golgb1	UTSW	16	36,925,763 (GRCm38)	missense	probably benign	0.02
R5740:Golgb1	UTSW	16	36,919,000 (GRCm38)	missense	probably damaging	0.99
R5862:Golgb1	UTSW	16	36,926,091 (GRCm38)	splice site	silent
R5928:Golgb1	UTSW	16	36,911,987 (GRCm38)	missense	probably damaging	1.00
R6009:Golgb1	UTSW	16	36,914,959 (GRCm38)	missense	probably damaging	1.00
R6062:Golgb1	UTSW	16	36,914,671 (GRCm38)	missense	possibly damaging	0.89
R6102:Golgb1	UTSW	16	36,912,865 (GRCm38)	missense	probably damaging	1.00
R6198:Golgb1	UTSW	16	36,893,395 (GRCm38)	missense	probably damaging	1.00
R6253:Golgb1	UTSW	16	36,915,622 (GRCm38)	missense	possibly damaging	0.77
R6254:Golgb1	UTSW	16	36,913,978 (GRCm38)	missense	probably damaging	0.99
R6321:Golgb1	UTSW	16	36,918,197 (GRCm38)	nonsense	probably null
R6700:Golgb1	UTSW	16	36,875,584 (GRCm38)	intron	probably benign
R6870:Golgb1	UTSW	16	36,918,203 (GRCm38)	missense	probably damaging	1.00
R6882:Golgb1	UTSW	16	36,913,990 (GRCm38)	missense	probably benign
R6944:Golgb1	UTSW	16	36,912,113 (GRCm38)	missense	probably benign
R7108:Golgb1	UTSW	16	36,913,721 (GRCm38)	missense	probably benign	0.01
R7124:Golgb1	UTSW	16	36,913,673 (GRCm38)	missense	probably benign	0.01
R7125:Golgb1	UTSW	16	36,917,963 (GRCm38)	missense	possibly damaging	0.85
R7187:Golgb1	UTSW	16	36,916,150 (GRCm38)	missense	probably benign	0.43
R7205:Golgb1	UTSW	16	36,875,301 (GRCm38)	missense	unknown
R7206:Golgb1	UTSW	16	36,913,749 (GRCm38)	missense	probably benign	0.41
R7233:Golgb1	UTSW	16	36,914,758 (GRCm38)	missense	possibly damaging	0.91
R7320:Golgb1	UTSW	16	36,915,951 (GRCm38)	nonsense	probably null
R7367:Golgb1	UTSW	16	36,898,546 (GRCm38)	missense	probably benign	0.00
R7408:Golgb1	UTSW	16	36,898,547 (GRCm38)	missense	probably damaging	0.98
R7419:Golgb1	UTSW	16	36,912,919 (GRCm38)	missense	possibly damaging	0.95
R7556:Golgb1	UTSW	16	36,915,793 (GRCm38)	missense	probably benign	0.03
R7599:Golgb1	UTSW	16	36,875,396 (GRCm38)	missense	unknown
R7673:Golgb1	UTSW	16	36,913,669 (GRCm38)	missense	probably benign	0.05
R7789:Golgb1	UTSW	16	36,875,399 (GRCm38)	missense	unknown
R7792:Golgb1	UTSW	16	36,918,730 (GRCm38)	missense	probably benign	0.43
R7830:Golgb1	UTSW	16	36,898,721 (GRCm38)	missense	possibly damaging	0.93
R7847:Golgb1	UTSW	16	36,931,920 (GRCm38)	missense	probably damaging	1.00
R7905:Golgb1	UTSW	16	36,913,685 (GRCm38)	missense	probably benign
R7944:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7945:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7950:Golgb1	UTSW	16	36,915,424 (GRCm38)	missense	probably benign	0.13
R8040:Golgb1	UTSW	16	36,913,479 (GRCm38)	missense	possibly damaging	0.85
R8077:Golgb1	UTSW	16	36,918,633 (GRCm38)	missense	probably damaging	0.99
R8181:Golgb1	UTSW	16	36,916,830 (GRCm38)	missense	probably damaging	1.00
R8370:Golgb1	UTSW	16	36,912,317 (GRCm38)	missense	probably benign	0.00
R8684:Golgb1	UTSW	16	36,914,402 (GRCm38)	missense	possibly damaging	0.92
R8725:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8727:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8738:Golgb1	UTSW	16	36,916,313 (GRCm38)	missense	probably damaging	1.00
R8785:Golgb1	UTSW	16	36,919,744 (GRCm38)	missense	probably damaging	0.99
R8824:Golgb1	UTSW	16	36,915,689 (GRCm38)	missense	probably benign
R8825:Golgb1	UTSW	16	36,919,447 (GRCm38)	missense	probably benign	0.00
R8940:Golgb1	UTSW	16	36,916,397 (GRCm38)	missense	probably damaging	1.00
R8962:Golgb1	UTSW	16	36,913,616 (GRCm38)	missense	probably damaging	1.00
R9245:Golgb1	UTSW	16	36,918,819 (GRCm38)	nonsense	probably null
R9365:Golgb1	UTSW	16	36,915,762 (GRCm38)	missense	probably damaging	1.00
R9612:Golgb1	UTSW	16	36,919,605 (GRCm38)	missense	probably benign	0.41
R9620:Golgb1	UTSW	16	36,919,449 (GRCm38)	missense	probably benign
R9691:Golgb1	UTSW	16	36,898,634 (GRCm38)	missense	probably damaging	1.00
R9747:Golgb1	UTSW	16	36,893,407 (GRCm38)	missense	probably damaging	1.00
V1662:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
X0067:Golgb1	UTSW	16	36,914,303 (GRCm38)	nonsense	probably null
Z1088:Golgb1	UTSW	16	36,919,742 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGCTAAATATGGCTCTCTCCTC -3'
(R):5'- GAGTCCTGCCACTCTTTCAG -3'

Sequencing Primer
(F):5'- TCCCAGAAGAGAACTGCAGATCTG -3'
(R):5'- ACTCTTTCAGCTGGGCCAG -3'

Posted On

2016-06-15