Mutagenetix > Incidental Mutation

Incidental Mutation 'R5053:Or8k25'

394723

Institutional Source

Beutler Lab

Gene Symbol

Or8k25

Ensembl Gene

ENSMUSG00000075185

Gene Name

olfactory receptor family 8 subfamily K member 25

Synonyms

MOR188-1, MOR188-9, Olfr1515, Olfr1061, MOR188-1, MOR188-7, GA_x6K02T2Q125-47883395-47882454

MMRRC Submission

042643-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R5053 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86243453-86244394 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 86243682 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 238 (F238S)

Ref Sequence

ENSEMBL: ENSMUSP00000097474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099889]

AlphaFold

Q7TR72

Predicted Effect

probably damaging
Transcript: ENSMUST00000099889
AA Change: F238S

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000097474
Gene: ENSMUSG00000075185
AA Change: F238S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.5e-51	PFAM
Pfam:7tm_1	41	291	2.2e-17	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.3%
10x: 95.9%
20x: 90.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acvrl1	A	T	15: 101,035,250 (GRCm39)	I259F	probably damaging	Het
Adgrf2	A	G	17: 43,021,334 (GRCm39)	S497P	probably damaging	Het
Adsl	T	C	15: 80,844,651 (GRCm39)	C180R	probably damaging	Het
Ahctf1	A	G	1: 179,614,349 (GRCm39)	I448T	possibly damaging	Het
Alg9	G	T	9: 50,699,472 (GRCm39)	G200W	probably damaging	Het
Amhr2	G	A	15: 102,355,693 (GRCm39)	V213I	probably damaging	Het
Ankrd44	A	T	1: 54,774,248 (GRCm39)	L200*	probably null	Het
As3mt	A	G	19: 46,697,493 (GRCm39)	Y87C	probably damaging	Het
Bcl11a	C	A	11: 24,114,068 (GRCm39)	F470L	probably benign	Het
Cacna2d2	A	T	9: 107,392,063 (GRCm39)	Y468F	probably damaging	Het
Cep250	A	G	2: 155,804,848 (GRCm39)	D71G	possibly damaging	Het
Clca4b	T	C	3: 144,616,882 (GRCm39)	T923A	probably benign	Het
Clec16a	T	C	16: 10,394,461 (GRCm39)	L273P	probably damaging	Het
Comtd1	T	C	14: 21,897,764 (GRCm39)	D123G	probably damaging	Het
Cpa5	A	T	6: 30,623,271 (GRCm39)	E144V	probably damaging	Het
Cyp2c37	G	A	19: 39,990,331 (GRCm39)	S343N	probably benign	Het
Dennd6a	C	A	14: 26,329,738 (GRCm39)	T103K	probably damaging	Het
Ect2l	A	G	10: 18,016,093 (GRCm39)	L733P	probably damaging	Het
Erg	T	A	16: 95,325,393 (GRCm39)	H11L	probably benign	Het
Ero1b	T	C	13: 12,614,795 (GRCm39)	L253P	probably damaging	Het
Etfbkmt	T	A	6: 149,048,766 (GRCm39)	L135*	probably null	Het
Frmd4a	A	T	2: 4,608,732 (GRCm39)	T867S	probably damaging	Het
Gm815	C	T	19: 26,863,813 (GRCm39)	A18V	unknown	Het
Gna12	C	A	5: 140,746,482 (GRCm39)	R321L	probably benign	Het
Herc1	A	T	9: 66,377,608 (GRCm39)	K3458M	possibly damaging	Het
Irs1	A	G	1: 82,264,643 (GRCm39)	L1191P	probably benign	Het
Kcp	G	T	6: 29,496,957 (GRCm39)	Q551K	probably benign	Het
Klhl11	C	T	11: 100,363,026 (GRCm39)	V177M	probably damaging	Het
Klk14	G	A	7: 43,341,501 (GRCm39)	C51Y	probably damaging	Het
Llcfc1	A	G	6: 41,662,269 (GRCm39)	H91R	possibly damaging	Het
Lmnb2	A	T	10: 80,740,489 (GRCm39)	M223K	probably damaging	Het
Mbd6	C	T	10: 127,122,310 (GRCm39)	V173I	probably benign	Het
Mme	T	A	3: 63,272,270 (GRCm39)	V623E	probably damaging	Het
Ndc1	T	C	4: 107,231,415 (GRCm39)	S119P	probably damaging	Het
Nfs1	A	C	2: 155,968,318 (GRCm39)	C383G	probably damaging	Het
Nphp4	A	G	4: 152,628,919 (GRCm39)		probably null	Het
Oca2	C	A	7: 55,973,328 (GRCm39)	Q411K	probably benign	Het
Or10ab4	A	G	7: 107,654,741 (GRCm39)	H184R	probably benign	Het
Or11h23	T	A	14: 50,947,968 (GRCm39)	Y60*	probably null	Het
Or1ad1	G	A	11: 50,876,137 (GRCm39)	G203D	probably damaging	Het
Or4c15	C	A	2: 88,760,597 (GRCm39)	V21F	probably benign	Het
Pcdh7	A	T	5: 57,878,943 (GRCm39)	T493S	probably damaging	Het
Phf20	T	C	2: 156,115,782 (GRCm39)	S368P	probably benign	Het
Pla2g2e	T	G	4: 138,607,686 (GRCm39)	V22G	possibly damaging	Het
Plxna1	A	T	6: 89,299,442 (GRCm39)	H1736Q	probably damaging	Het
Prlr	C	A	15: 10,325,471 (GRCm39)	Q215K	probably benign	Het
Prpf6	T	C	2: 181,291,246 (GRCm39)	V687A	probably benign	Het
Ptprq	T	A	10: 107,399,063 (GRCm39)	T1951S	probably damaging	Het
Sec31a	G	T	5: 100,541,073 (GRCm39)	Q345K	possibly damaging	Het
Serpina3k	T	C	12: 104,309,473 (GRCm39)		probably null	Het
Sertad3	T	A	7: 27,175,947 (GRCm39)	V127E	probably benign	Het
Sf3b1	A	T	1: 55,036,336 (GRCm39)	N912K	probably benign	Het
Shq1	A	G	6: 100,632,220 (GRCm39)	L202P	probably damaging	Het
Sp8	C	T	12: 118,813,339 (GRCm39)	T398M	probably damaging	Het
Tmem150c	A	T	5: 100,231,599 (GRCm39)	D125E	probably benign	Het
Trim3	G	T	7: 105,266,968 (GRCm39)	D470E	probably damaging	Het
Ubap1	T	A	4: 41,387,315 (GRCm39)	C88*	probably null	Het
Unc79	T	A	12: 103,071,007 (GRCm39)	D1124E	probably damaging	Het
Usf3	T	C	16: 44,037,550 (GRCm39)	S677P	probably benign	Het
Vmn1r76	T	C	7: 11,664,241 (GRCm39)		probably null	Het
Zfp819	C	T	7: 43,266,570 (GRCm39)	T275I	probably damaging	Het
Zranb2	T	A	3: 157,246,796 (GRCm39)	D161E	probably damaging	Het

Other mutations in Or8k25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01714:Or8k25	APN	2	86,244,144 (GRCm39)	missense	probably benign	0.35
IGL01721:Or8k25	APN	2	86,243,677 (GRCm39)	missense	probably damaging	1.00
IGL02696:Or8k25	APN	2	86,243,959 (GRCm39)	missense	probably benign	0.03
BB007:Or8k25	UTSW	2	86,243,560 (GRCm39)	missense	probably damaging	1.00
BB017:Or8k25	UTSW	2	86,243,560 (GRCm39)	missense	probably damaging	1.00
R0366:Or8k25	UTSW	2	86,244,369 (GRCm39)	missense	possibly damaging	0.71
R0607:Or8k25	UTSW	2	86,243,514 (GRCm39)	missense	probably damaging	1.00
R1013:Or8k25	UTSW	2	86,244,319 (GRCm39)	missense	possibly damaging	0.61
R1017:Or8k25	UTSW	2	86,243,855 (GRCm39)	missense	probably damaging	1.00
R1617:Or8k25	UTSW	2	86,244,035 (GRCm39)	nonsense	probably null
R1690:Or8k25	UTSW	2	86,244,298 (GRCm39)	missense	probably benign	0.03
R4126:Or8k25	UTSW	2	86,243,568 (GRCm39)	missense	probably damaging	0.99
R5443:Or8k25	UTSW	2	86,243,937 (GRCm39)	missense	possibly damaging	0.54
R6195:Or8k25	UTSW	2	86,243,551 (GRCm39)	missense	probably damaging	0.98
R6233:Or8k25	UTSW	2	86,243,551 (GRCm39)	missense	probably damaging	0.98
R6468:Or8k25	UTSW	2	86,244,381 (GRCm39)	missense	probably damaging	0.99
R7188:Or8k25	UTSW	2	86,243,695 (GRCm39)	nonsense	probably null
R7300:Or8k25	UTSW	2	86,244,330 (GRCm39)	missense	probably null	0.27
R7374:Or8k25	UTSW	2	86,244,196 (GRCm39)	missense	probably benign	0.39
R7392:Or8k25	UTSW	2	86,243,496 (GRCm39)	missense	probably benign
R7494:Or8k25	UTSW	2	86,243,592 (GRCm39)	missense	probably benign	0.24
R7930:Or8k25	UTSW	2	86,243,560 (GRCm39)	missense	probably damaging	1.00
R8491:Or8k25	UTSW	2	86,244,099 (GRCm39)	missense	probably benign	0.00
R8680:Or8k25	UTSW	2	86,243,935 (GRCm39)	missense	probably benign	0.16
R8848:Or8k25	UTSW	2	86,243,821 (GRCm39)	missense	probably benign	0.05
R9175:Or8k25	UTSW	2	86,244,099 (GRCm39)	missense	probably benign	0.00
X0023:Or8k25	UTSW	2	86,244,303 (GRCm39)	missense	probably benign	0.29
Z1176:Or8k25	UTSW	2	86,243,872 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TGTTCCTCAAGCTGTATGTTAAGG -3'
(R):5'- TGGCCATCTGTAAACCACTCC -3'

Sequencing Primer
(F):5'- CCTCAAGCTGTATGTTAAGGGATTC -3'
(R):5'- TGTGGCTACAATGTCATCAGTC -3'

Posted On

2016-06-15