Incidental Mutation 'R5129:Zfp874a'
ID394920
Institutional Source Beutler Lab
Gene Symbol Zfp874a
Ensembl Gene ENSMUSG00000069206
Gene Namezinc finger protein 874a
SynonymsZfp874, Rslcan15, C330011K17Rik
MMRRC Submission 042717-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.129) question?
Stock #R5129 (G1)
Quality Score225
Status Validated
Chromosome13
Chromosomal Location67424549-67451624 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 67442981 bp
ZygosityHeterozygous
Amino Acid Change Cysteine to Serine at position 195 (C195S)
Ref Sequence ENSEMBL: ENSMUSP00000074735 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000057241] [ENSMUST00000075255] [ENSMUST00000180580] [ENSMUST00000223682] [ENSMUST00000225479]
Predicted Effect probably benign
Transcript: ENSMUST00000057241
SMART Domains Protein: ENSMUSP00000077307
Gene: ENSMUSG00000069206

DomainStartEndE-ValueType
KRAB 5 65 1.73e-27 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000075255
AA Change: C195S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000074735
Gene: ENSMUSG00000069206
AA Change: C195S

DomainStartEndE-ValueType
KRAB 5 65 1.73e-27 SMART
ZnF_C2H2 137 159 9.58e-3 SMART
ZnF_C2H2 165 187 5.99e-4 SMART
ZnF_C2H2 193 215 7.26e-3 SMART
ZnF_C2H2 221 243 2.4e-3 SMART
ZnF_C2H2 249 269 1.88e2 SMART
ZnF_C2H2 277 299 5.21e-4 SMART
ZnF_C2H2 305 327 2.12e-4 SMART
ZnF_C2H2 333 355 2.12e-4 SMART
ZnF_C2H2 361 383 8.47e-4 SMART
ZnF_C2H2 389 411 1.79e-2 SMART
ZnF_C2H2 417 438 2.86e-1 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000180580
SMART Domains Protein: ENSMUSP00000137953
Gene: ENSMUSG00000069206

DomainStartEndE-ValueType
KRAB 5 65 1.73e-27 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000223682
Predicted Effect noncoding transcript
Transcript: ENSMUST00000224239
Predicted Effect probably benign
Transcript: ENSMUST00000225479
Predicted Effect probably benign
Transcript: ENSMUST00000225778
Meta Mutation Damage Score 0.3288 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.8%
  • 20x: 93.8%
Validation Efficiency 98% (51/52)
Allele List at MGI
Other mutations in this stock
Total: 48 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930432E11Rik A G 7: 29,561,361 noncoding transcript Het
Abcf1 C T 17: 35,960,795 probably benign Het
Adamts14 T A 10: 61,249,618 D209V probably benign Het
Aldh3b1 A G 19: 3,915,336 F392L probably benign Het
Atp1a2 A T 1: 172,275,955 D999E probably benign Het
Capn7 T C 14: 31,344,511 V94A probably damaging Het
Cdh13 A G 8: 119,095,215 D271G probably damaging Het
Cntnap5b G A 1: 100,379,090 G844D probably damaging Het
Col6a4 T C 9: 106,013,377 E1906G probably damaging Het
Daw1 T C 1: 83,205,903 Y225H probably damaging Het
Dvl3 T A 16: 20,517,340 M49K possibly damaging Het
Eef1b2 T G 1: 63,179,580 S175A probably damaging Het
Epb41l1 A T 2: 156,509,281 Y425F possibly damaging Het
Fam43b A T 4: 138,395,472 L179* probably null Het
Fbxw28 A G 9: 109,326,603 L314P probably damaging Het
Fstl4 C A 11: 53,186,439 D674E probably damaging Het
G3bp1 T C 11: 55,489,116 V92A possibly damaging Het
Gm3604 A T 13: 62,369,774 Y257N probably benign Het
Gm5415 C A 1: 32,545,479 R450M probably damaging Het
Gm5415 T A 1: 32,545,480 R450W probably damaging Het
Gm5900 A G 7: 104,950,016 noncoding transcript Het
Hectd4 G A 5: 121,343,510 V3041M possibly damaging Het
Hivep2 A G 10: 14,130,864 K1069E probably damaging Het
Hlf T C 11: 90,390,252 D38G probably benign Het
Hsd3b7 C A 7: 127,801,134 C18* probably null Het
Kdm5a T C 6: 120,405,022 C676R probably damaging Het
Klhdc7b A G 15: 89,388,548 Y1211C probably damaging Het
Krt78 C A 15: 101,947,580 V599L possibly damaging Het
Mroh9 C G 1: 163,060,760 G249R probably damaging Het
Nbas T C 12: 13,390,960 L1097P probably damaging Het
Nf2 A C 11: 4,816,145 D87E probably benign Het
Olfr113 T C 17: 37,575,180 Y81C probably damaging Het
Olfr961 T A 9: 39,647,494 I256N probably benign Het
Ppil3 A G 1: 58,440,833 probably benign Het
Prrc2a T C 17: 35,160,178 E276G unknown Het
Spata16 A G 3: 26,667,564 E78G probably damaging Het
Tlr3 A T 8: 45,402,981 I54K probably damaging Het
Tmem72 A G 6: 116,702,013 L34P probably damaging Het
Triobp A G 15: 78,961,096 R213G probably benign Het
Tyrp1 T A 4: 80,846,607 V7D probably damaging Het
Uhrf2 A G 19: 30,075,221 I372V probably benign Het
Uty T C Y: 1,158,592 T484A probably benign Het
Vcan T C 13: 89,690,240 D2395G probably damaging Het
Vmn1r225 T A 17: 20,503,116 I273N probably damaging Het
Zfp451 C T 1: 33,802,933 probably benign Het
Zfp457 T C 13: 67,293,356 E385G probably benign Het
Zfp74 C T 7: 29,932,455 M121I probably benign Het
Zfp866 A T 8: 69,767,709 probably null Het
Other mutations in Zfp874a
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02490:Zfp874a APN 13 67442700 nonsense probably null
R1052:Zfp874a UTSW 13 67442420 missense possibly damaging 0.89
R1991:Zfp874a UTSW 13 67442504 missense probably benign 0.03
R2085:Zfp874a UTSW 13 67442848 missense probably damaging 1.00
R2103:Zfp874a UTSW 13 67442504 missense probably benign 0.03
R5336:Zfp874a UTSW 13 67442680 missense probably damaging 1.00
R5423:Zfp874a UTSW 13 67442354 missense possibly damaging 0.75
R5713:Zfp874a UTSW 13 67449357 missense probably benign 0.16
R5855:Zfp874a UTSW 13 67442693 missense probably benign 0.03
R6496:Zfp874a UTSW 13 67442575 missense possibly damaging 0.62
R7046:Zfp874a UTSW 13 67442299 missense probably damaging 1.00
R7065:Zfp874a UTSW 13 67442282 missense probably damaging 0.97
R7233:Zfp874a UTSW 13 67442657 missense possibly damaging 0.83
R7271:Zfp874a UTSW 13 67443296 missense probably benign 0.00
R7465:Zfp874a UTSW 13 67442257 missense probably damaging 0.99
R7468:Zfp874a UTSW 13 67425604 utr 3 prime probably null
X0063:Zfp874a UTSW 13 67442987 missense probably damaging 1.00
Z1177:Zfp874a UTSW 13 67442662 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCCCTAAGATATGAACGATAGCAAAAG -3'
(R):5'- GATCACAGCTCTCTAAGTATGCAATG -3'

Sequencing Primer
(F):5'- AGGATTTGCCACATTCTTGACAC -3'
(R):5'- GCTCTCTAAGTATGCAATGCCAGAG -3'
Posted On2016-06-21