Mutagenetix > Incidental Mutations

Incidental Mutation 'R5130:Cry2'

394938

Institutional Source

Beutler Lab

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome 2 (photolyase-like)

Synonyms

MMRRC Submission

042718-MU

Accession Numbers

Is this an essential gene?

Probably essential (E-score: 0.790) question?

Stock #

R5130 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

92403646-92434043 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 92424599 bp

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 137 (E137G)

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

Predicted Effect

probably benign
Transcript: ENSMUST00000090559
AA Change: E137G

PolyPhen 2 Score 0.279 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: E137G

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000111278
AA Change: E137G

PolyPhen 2 Score 0.279 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: E137G

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125488

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144842

Meta Mutation Damage Score

0.384

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.4%

Validation Efficiency

96% (51/53)

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930558K02Rik	T	C	1: 161,952,615	N110S	possibly damaging	Het
4932431P20Rik	T	C	7: 29,529,274		noncoding transcript	Het
Acss3	T	C	10: 107,004,725	I392V	possibly damaging	Het
Agbl5	G	A	5: 30,903,059	R141Q	probably damaging	Het
Arhgef11	A	G	3: 87,726,014	H696R	possibly damaging	Het
Baiap3	G	T	17: 25,245,342	D847E	probably benign	Het
Cdc27	T	C	11: 104,534,774	K72R	probably benign	Het
Dhodh	A	T	8: 109,595,756	L237Q	possibly damaging	Het
Dscam	A	T	16: 96,819,779	N576K	probably benign	Het
Eng	A	G	2: 32,681,506	N636S	probably damaging	Het
Ephx2	A	T	14: 66,108,062	I151K	probably damaging	Het
Fahd1	A	G	17: 24,849,759	C115R	probably damaging	Het
Fer1l4	T	A	2: 156,049,466	I143F	possibly damaging	Het
Iqgap3	C	A	3: 88,108,854	N981K	probably damaging	Het
Kcna5	T	C	6: 126,534,533	I211V	probably benign	Het
Mcm9	A	T	10: 53,630,399	V14E	possibly damaging	Het
Mlh1	A	G	9: 111,229,838		probably null	Het
Mylk	A	G	16: 34,988,997	K1775E	probably damaging	Het
Myo18b	T	C	5: 112,873,903	D541G	probably benign	Het
Ncapd2	A	T	6: 125,169,924	M1233K	possibly damaging	Het
Nf2	A	T	11: 4,829,862		probably benign	Het
Nova2	C	T	7: 18,926,144	T22I	unknown	Het
Olfr411	A	T	11: 74,347,167	M19K	probably damaging	Het
Olfr846	T	A	9: 19,361,073	Y94F	possibly damaging	Het
Olfr874	T	A	9: 37,746,509	I125N	probably damaging	Het
Pcdha2	A	T	18: 36,940,669	N451I	probably damaging	Het
Pcdhb12	T	A	18: 37,435,824	F8I	probably benign	Het
Pdzph1	A	C	17: 58,922,609	L1018W	probably damaging	Het
Prdm9	T	C	17: 15,544,467	R684G	probably benign	Het
Rabep1	T	A	11: 70,904,731	V261E	probably damaging	Het
Ros1	T	A	10: 52,163,941	Y318F	possibly damaging	Het
Samd9l	A	T	6: 3,374,548	D904E	possibly damaging	Het
Siah3	A	T	14: 75,525,941	K211*	probably null	Het
Slc5a8	T	A	10: 88,926,215	N572K	probably benign	Het
Slfn8	A	G	11: 83,003,821	F664S	probably benign	Het
Sowahb	T	A	5: 93,042,771	K696N	possibly damaging	Het
St7l	A	T	3: 104,895,764	H330L	probably damaging	Het
Tnrc6c	T	A	11: 117,738,350		probably null	Het
Ttc28	G	T	5: 110,892,856	V37F	probably benign	Het
Uaca	C	A	9: 60,880,228	Q1409K	probably damaging	Het
V1rd19	T	A	7: 24,003,112	M1K	probably null	Het
Vmn1r225	A	G	17: 20,502,785	R163G	possibly damaging	Het
Zc3h3	C	A	15: 75,779,290	V694L	probably damaging	Het
Zfp442	G	T	2: 150,409,610	T123K	possibly damaging	Het
Zmym1	A	T	4: 127,048,658	S646T	probably damaging	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01995:Cry2	APN	2	92424632	missense	probably benign	0.15
IGL02167:Cry2	APN	2	92433821	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92413039	missense	probably damaging	0.99
IGL02343:Cry2	APN	2	92426921	missense	possibly damaging	0.90
IGL02432:Cry2	APN	2	92413667	missense	probably damaging	0.99
IGL02725:Cry2	APN	2	92413260	splice site	probably benign
IGL02932:Cry2	APN	2	92413117	nonsense	probably null
IGL03122:Cry2	APN	2	92413295	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92413715	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R1325:Cry2	UTSW	2	92413770	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92424566	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92413640	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92411692	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92413827	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92424554	missense	probably benign	0.38
R5145:Cry2	UTSW	2	92413060	missense	probably benign
R5970:Cry2	UTSW	2	92412967	missense	probably benign	0.08
R6179:Cry2	UTSW	2	92413842	missense	probably damaging	0.98
R7102:Cry2	UTSW	2	92413093	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92413659	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92412981	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92413664	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92413638	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92413005	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TCCAAATCGGGTTAACCTCTTGG -3'
(R):5'- TAGCACAGCTCAAGGTCAGC -3'

Sequencing Primer
(F):5'- GGTGACTTCTTTAAGCCCCCAAC -3'
(R):5'- AAGGTCAGCCCTGCTCACTC -3'

Posted On

2016-06-21