Mutagenetix > Incidental Mutation

Incidental Mutation 'R5148:Vmn2r6'

395190

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r6

Ensembl Gene

ENSMUSG00000090581

Gene Name

vomeronasal 2, receptor 6

Synonyms

EG620718, EG667069

MMRRC Submission

043262-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.184) question?

Stock #

R5148 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64537561-64565298 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 64556594 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 273 (V273G)

Ref Sequence

ENSEMBL: ENSMUSP00000135148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165012] [ENSMUST00000176481]

AlphaFold

H3BK29

Predicted Effect

probably damaging
Transcript: ENSMUST00000165012
AA Change: V184G

PolyPhen 2 Score 0.961 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000131831
Gene: ENSMUSG00000090581
AA Change: V184G

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	1.4e-72	PFAM
Pfam:Peripla_BP_6	58	244	1.2e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	3.9e-76	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000176481
AA Change: V273G

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000135148
Gene: ENSMUSG00000090581
AA Change: V273G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	9.8e-77	PFAM
Pfam:Peripla_BP_6	142	331	3.4e-10	PFAM
Pfam:NCD3G	547	600	5.4e-17	PFAM
Pfam:7tm_3	633	867	3.9e-47	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.2%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ank2	A	T	3: 127,025,636 (GRCm38)		probably null	Het
Atf7	A	G	15: 102,547,173 (GRCm38)	M252T	probably benign	Het
Cacna1h	C	A	17: 25,387,545 (GRCm38)	D1027Y	probably damaging	Het
Cngb1	A	G	8: 95,265,983 (GRCm38)	V667A	probably benign	Het
Cntf	T	C	19: 12,764,004 (GRCm38)	E164G	probably damaging	Het
Dbt	T	C	3: 116,528,244 (GRCm38)		probably benign	Het
Egfem1	G	A	3: 29,457,823 (GRCm38)		probably benign	Het
Gm12790	C	T	4: 101,968,071 (GRCm38)	V49I	possibly damaging	Het
Gm5444	C	T	13: 4,834,315 (GRCm38)		noncoding transcript	Het
Gpcpd1	A	T	2: 132,534,190 (GRCm38)	Y574*	probably null	Het
Gss	G	T	2: 155,573,109 (GRCm38)	N225K	possibly damaging	Het
Il17rc	G	T	6: 113,482,997 (GRCm38)	A635S	probably benign	Het
Lhfpl5	T	A	17: 28,579,968 (GRCm38)	D150E	probably damaging	Het
Lin9	T	A	1: 180,669,198 (GRCm38)	L351I	probably benign	Het
Lrrc40	T	G	3: 158,054,569 (GRCm38)		probably null	Het
Ly6f	A	G	15: 75,271,797 (GRCm38)	T118A	probably benign	Het
Malrd1	A	G	2: 16,142,226 (GRCm38)	N1960D	unknown	Het
Map3k14	T	C	11: 103,239,332 (GRCm38)	H253R	probably benign	Het
Morc2a	T	C	11: 3,689,084 (GRCm38)	L1025P	probably damaging	Het
Nlrc5	G	T	8: 94,476,693 (GRCm38)	G474W	probably damaging	Het
Olfr1162	G	A	2: 88,050,393 (GRCm38)	T77I	probably benign	Het
Olfr1487	C	A	19: 13,619,510 (GRCm38)	S116*	probably null	Het
Olfr894	T	C	9: 38,219,021 (GRCm38)	I66T	probably benign	Het
Olr1	G	T	6: 129,493,609 (GRCm38)	D198E	probably benign	Het
Pafah1b1	A	T	11: 74,684,452 (GRCm38)	S209T	probably damaging	Het
Phf14	A	T	6: 11,961,642 (GRCm38)	Y426F	possibly damaging	Het
Phldb1	A	G	9: 44,704,158 (GRCm38)	V855A	probably benign	Het
Pira2	T	G	7: 3,844,593 (GRCm38)	R32S	possibly damaging	Het
Pnldc1	T	C	17: 12,892,789 (GRCm38)	I344V	probably benign	Het
Prss29	T	C	17: 25,320,907 (GRCm38)	V93A	probably benign	Het
Ptpn13	C	T	5: 103,492,232 (GRCm38)	L186F	probably damaging	Het
Ralgps1	A	C	2: 33,158,987 (GRCm38)	C303W	probably damaging	Het
Sdr42e1	T	A	8: 117,663,603 (GRCm38)	N100Y	probably damaging	Het
Serpina11	A	T	12: 103,986,244 (GRCm38)	L96Q	probably damaging	Het
Slc12a8	G	A	16: 33,624,918 (GRCm38)	R448H	probably benign	Het
Snrpd1	T	A	18: 10,626,892 (GRCm38)	V53E	probably benign	Het
Ssbp1	T	C	6: 40,477,949 (GRCm38)	V114A	possibly damaging	Het
T	A	G	17: 8,436,205 (GRCm38)	E47G	probably damaging	Het
Tmem156	T	C	5: 65,073,768 (GRCm38)	K189R	probably benign	Het
Trim47	G	T	11: 116,107,852 (GRCm38)	Q314K	possibly damaging	Het
Vmn2r3	G	T	3: 64,278,826 (GRCm38)	P146Q	probably damaging	Het
Wrnip1	G	T	13: 32,806,856 (GRCm38)	R366L	probably damaging	Het
Zfp981	A	T	4: 146,536,900 (GRCm38)	H94L	possibly damaging	Het

Other mutations in Vmn2r6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01547:Vmn2r6	APN	3	64,538,104 (GRCm38)	missense	probably damaging	1.00
IGL01968:Vmn2r6	APN	3	64,556,345 (GRCm38)	missense	possibly damaging	0.94
IGL02009:Vmn2r6	APN	3	64,537,902 (GRCm38)	missense	possibly damaging	0.61
IGL02039:Vmn2r6	APN	3	64,556,189 (GRCm38)	missense	probably damaging	1.00
IGL02652:Vmn2r6	APN	3	64,556,328 (GRCm38)	missense	probably benign	0.24
IGL02737:Vmn2r6	APN	3	64,556,490 (GRCm38)	missense	possibly damaging	0.55
IGL02808:Vmn2r6	APN	3	64,556,496 (GRCm38)	missense	probably damaging	1.00
IGL03066:Vmn2r6	APN	3	64,565,153 (GRCm38)	missense	probably damaging	0.99
IGL03331:Vmn2r6	APN	3	64,538,007 (GRCm38)	missense	probably damaging	1.00
BB010:Vmn2r6	UTSW	3	64,559,803 (GRCm38)	missense	probably benign	0.02
BB020:Vmn2r6	UTSW	3	64,559,803 (GRCm38)	missense	probably benign	0.02
R0010:Vmn2r6	UTSW	3	64,559,545 (GRCm38)	nonsense	probably null
R0206:Vmn2r6	UTSW	3	64,539,912 (GRCm38)	missense	probably benign
R0206:Vmn2r6	UTSW	3	64,539,912 (GRCm38)	missense	probably benign
R0208:Vmn2r6	UTSW	3	64,539,912 (GRCm38)	missense	probably benign
R0427:Vmn2r6	UTSW	3	64,559,587 (GRCm38)	missense	probably damaging	1.00
R0466:Vmn2r6	UTSW	3	64,556,302 (GRCm38)	missense	probably damaging	1.00
R1018:Vmn2r6	UTSW	3	64,556,840 (GRCm38)	missense	probably benign	0.00
R1104:Vmn2r6	UTSW	3	64,538,066 (GRCm38)	missense	possibly damaging	0.93
R1186:Vmn2r6	UTSW	3	64,565,067 (GRCm38)	missense	probably benign	0.01
R1245:Vmn2r6	UTSW	3	64,556,790 (GRCm38)	missense	possibly damaging	0.53
R1295:Vmn2r6	UTSW	3	64,538,273 (GRCm38)	missense	probably damaging	1.00
R1473:Vmn2r6	UTSW	3	64,538,158 (GRCm38)	nonsense	probably null
R1498:Vmn2r6	UTSW	3	64,556,469 (GRCm38)	missense	probably damaging	1.00
R1925:Vmn2r6	UTSW	3	64,556,277 (GRCm38)	missense	possibly damaging	0.87
R2044:Vmn2r6	UTSW	3	64,537,841 (GRCm38)	missense	probably damaging	0.96
R2069:Vmn2r6	UTSW	3	64,556,098 (GRCm38)	missense	possibly damaging	0.89
R2253:Vmn2r6	UTSW	3	64,559,718 (GRCm38)	missense	probably damaging	1.00
R2261:Vmn2r6	UTSW	3	64,556,669 (GRCm38)	missense	probably benign	0.24
R2262:Vmn2r6	UTSW	3	64,556,669 (GRCm38)	missense	probably benign	0.24
R2350:Vmn2r6	UTSW	3	64,556,352 (GRCm38)	missense	probably benign	0.01
R2680:Vmn2r6	UTSW	3	64,538,286 (GRCm38)	missense	possibly damaging	0.91
R2846:Vmn2r6	UTSW	3	64,556,790 (GRCm38)	missense	possibly damaging	0.53
R2860:Vmn2r6	UTSW	3	64,547,339 (GRCm38)	missense	probably benign	0.00
R2861:Vmn2r6	UTSW	3	64,547,339 (GRCm38)	missense	probably benign	0.00
R3766:Vmn2r6	UTSW	3	64,556,508 (GRCm38)	missense	probably benign	0.19
R3870:Vmn2r6	UTSW	3	64,556,621 (GRCm38)	missense	probably damaging	0.96
R4018:Vmn2r6	UTSW	3	64,556,472 (GRCm38)	missense	probably benign	0.05
R4024:Vmn2r6	UTSW	3	64,538,250 (GRCm38)	missense	possibly damaging	0.73
R4026:Vmn2r6	UTSW	3	64,538,250 (GRCm38)	missense	possibly damaging	0.73
R4227:Vmn2r6	UTSW	3	64,537,948 (GRCm38)	missense	probably damaging	0.99
R4526:Vmn2r6	UTSW	3	64,537,724 (GRCm38)	missense	probably benign	0.32
R4570:Vmn2r6	UTSW	3	64,559,647 (GRCm38)	missense	probably benign	0.31
R4894:Vmn2r6	UTSW	3	64,547,408 (GRCm38)	missense	probably benign
R4934:Vmn2r6	UTSW	3	64,556,345 (GRCm38)	missense	probably damaging	0.99
R5057:Vmn2r6	UTSW	3	64,537,786 (GRCm38)	missense	probably damaging	1.00
R5059:Vmn2r6	UTSW	3	64,537,623 (GRCm38)	missense	possibly damaging	0.89
R5155:Vmn2r6	UTSW	3	64,538,514 (GRCm38)	missense	probably benign	0.44
R5179:Vmn2r6	UTSW	3	64,537,990 (GRCm38)	missense	probably benign	0.00
R5256:Vmn2r6	UTSW	3	64,556,842 (GRCm38)	missense	probably benign	0.33
R5861:Vmn2r6	UTSW	3	64,556,033 (GRCm38)	missense	probably benign	0.00
R5950:Vmn2r6	UTSW	3	64,565,231 (GRCm38)	missense	probably benign	0.05
R6081:Vmn2r6	UTSW	3	64,556,532 (GRCm38)	missense	probably benign	0.25
R6173:Vmn2r6	UTSW	3	64,559,755 (GRCm38)	missense	probably damaging	1.00
R6190:Vmn2r6	UTSW	3	64,538,003 (GRCm38)	missense	probably benign	0.04
R6240:Vmn2r6	UTSW	3	64,556,805 (GRCm38)	missense	probably damaging	1.00
R6433:Vmn2r6	UTSW	3	64,547,380 (GRCm38)	nonsense	probably null
R6645:Vmn2r6	UTSW	3	64,556,876 (GRCm38)	missense	probably damaging	1.00
R6791:Vmn2r6	UTSW	3	64,538,159 (GRCm38)	missense	probably damaging	1.00
R7265:Vmn2r6	UTSW	3	64,556,774 (GRCm38)	missense	probably benign	0.00
R7503:Vmn2r6	UTSW	3	64,539,951 (GRCm38)	nonsense	probably null
R7562:Vmn2r6	UTSW	3	64,556,520 (GRCm38)	missense	probably benign	0.00
R7584:Vmn2r6	UTSW	3	64,565,262 (GRCm38)	missense	probably benign	0.07
R7611:Vmn2r6	UTSW	3	64,565,142 (GRCm38)	missense	probably damaging	0.98
R7759:Vmn2r6	UTSW	3	64,556,570 (GRCm38)	missense	probably damaging	1.00
R7834:Vmn2r6	UTSW	3	64,538,022 (GRCm38)	missense	probably damaging	1.00
R7933:Vmn2r6	UTSW	3	64,559,803 (GRCm38)	missense	probably benign	0.02
R7982:Vmn2r6	UTSW	3	64,559,820 (GRCm38)	missense	probably damaging	1.00
R8024:Vmn2r6	UTSW	3	64,559,824 (GRCm38)	missense	probably benign	0.40
R8074:Vmn2r6	UTSW	3	64,547,643 (GRCm38)	intron	probably benign
R8169:Vmn2r6	UTSW	3	64,539,889 (GRCm38)	missense	probably benign	0.01
R8337:Vmn2r6	UTSW	3	64,556,105 (GRCm38)	nonsense	probably null
R8736:Vmn2r6	UTSW	3	64,559,800 (GRCm38)	missense	probably damaging	1.00
R8962:Vmn2r6	UTSW	3	64,556,155 (GRCm38)	missense	probably damaging	1.00
R9139:Vmn2r6	UTSW	3	64,556,856 (GRCm38)	missense	probably benign	0.12
R9206:Vmn2r6	UTSW	3	64,559,611 (GRCm38)	missense	probably damaging	0.97
R9295:Vmn2r6	UTSW	3	64,556,063 (GRCm38)	missense	probably benign	0.00
R9332:Vmn2r6	UTSW	3	64,547,250 (GRCm38)	missense	probably benign	0.01
R9616:Vmn2r6	UTSW	3	64,538,303 (GRCm38)	missense	probably damaging	1.00
R9663:Vmn2r6	UTSW	3	64,556,128 (GRCm38)	missense	possibly damaging	0.90
R9685:Vmn2r6	UTSW	3	64,556,660 (GRCm38)	missense	probably benign	0.19
X0020:Vmn2r6	UTSW	3	64,538,450 (GRCm38)	missense	probably benign
X0066:Vmn2r6	UTSW	3	64,547,378 (GRCm38)	missense	probably damaging	1.00
Z1176:Vmn2r6	UTSW	3	64,556,325 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- ACACTTCTTGGTACTGCAAATCC -3'
(R):5'- AATTCCAGTCTGAGGCCATCG -3'

Sequencing Primer
(F):5'- GGTACTGCAAATCCAATAGTTCC -3'
(R):5'- GGCCATCGTGAATTTAATTAGACAC -3'

Posted On

2016-06-21