Mutagenetix > Incidental Mutation

Incidental Mutation 'R5153:Olfr804'

395537

Institutional Source

Beutler Lab

Gene Symbol

Olfr804

Ensembl Gene

ENSMUSG00000095401

Gene Name

olfactory receptor 804

Synonyms

MOR110-7, GA_x6K02T2PULF-11383575-11384519

MMRRC Submission

042735-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R5153 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

129703210-129710018 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 129705157 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 93 (N93S)

Ref Sequence

ENSEMBL: ENSMUSP00000150132 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077312] [ENSMUST00000213331]

AlphaFold

Q7TRH7

Predicted Effect

probably benign
Transcript: ENSMUST00000077312
AA Change: N93S

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000076540
Gene: ENSMUSG00000095401
AA Change: N93S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	6e-48	PFAM
Pfam:7tm_1	39	288	3.3e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213331
AA Change: N93S

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216747

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2300003K06Rik	A	T	11: 99,837,247	C257S	unknown	Het
4930596D02Rik	C	A	14: 35,810,255	R121L	probably benign	Het
5330417C22Rik	A	T	3: 108,472,747	Y349N	possibly damaging	Het
8030411F24Rik	A	G	2: 148,783,440	Q98R	probably benign	Het
9430007A20Rik	T	A	4: 144,522,267	M68K	probably benign	Het
Acin1	A	G	14: 54,645,613	V194A	probably benign	Het
Acsm1	T	C	7: 119,640,727	I298T	possibly damaging	Het
Aoc1	A	G	6: 48,908,747	N729D	probably benign	Het
Apobr	C	T	7: 126,587,732	T20I	possibly damaging	Het
Arsb	A	G	13: 93,940,598	E491G	probably benign	Het
AW209491	C	G	13: 14,637,179	Q206E	probably benign	Het
Camsap1	A	T	2: 25,933,618	N1473K	probably damaging	Het
Casp8	A	G	1: 58,844,845	Q457R	probably benign	Het
Ccdc154	G	A	17: 25,168,341	A350T	probably damaging	Het
Cecr2	A	G	6: 120,734,560	E183G	probably benign	Het
Cep295	A	T	9: 15,357,629	S39T	probably benign	Het
Cep350	A	T	1: 155,935,946	I416K	probably damaging	Het
Cidea	C	T	18: 67,367,420	T184M	probably damaging	Het
Cnot10	G	A	9: 114,613,735	A468V	probably damaging	Het
Defb22	A	T	2: 152,485,802	N154K	unknown	Het
Dnah17	A	T	11: 118,082,974	C1915*	probably null	Het
Dnah2	T	C	11: 69,520,933	T288A	possibly damaging	Het
Dopey2	T	A	16: 93,774,003	M1512K	probably damaging	Het
Dsp	T	G	13: 38,182,306	I572S	probably damaging	Het
Eepd1	A	G	9: 25,586,753	H378R	probably benign	Het
Emilin2	A	G	17: 71,273,502	M743T	possibly damaging	Het
Ext1	C	A	15: 53,075,817	W612L	probably damaging	Het
F2rl2	A	G	13: 95,697,112	T17A	probably benign	Het
Fbxl8	T	G	8: 105,267,107	C32G	probably damaging	Het
Fbxw17	A	G	13: 50,419,861	T38A	probably damaging	Het
Flii	A	T	11: 60,716,686	L882Q	possibly damaging	Het
Focad	T	G	4: 88,359,884	S1197A	unknown	Het
Gm6970	A	G	19: 47,170,917	V73A	probably damaging	Het
Heatr5b	G	A	17: 78,795,107	R1281*	probably null	Het
Khdc3	A	G	9: 73,103,438	Q190R	probably benign	Het
Krt9	T	A	11: 100,191,242	D244V	probably damaging	Het
Ldhd	T	C	8: 111,627,092	E463G	probably benign	Het
Lig4	A	T	8: 9,973,003	V259E	possibly damaging	Het
Luc7l3	A	T	11: 94,295,980		probably benign	Het
Man1a2	T	C	3: 100,656,263	E22G	probably damaging	Het
Mmp23	T	G	4: 155,651,340	D258A	probably damaging	Het
Msantd2	C	T	9: 37,523,213	R250*	probably null	Het
Mynn	T	C	3: 30,611,589	S457P	probably benign	Het
Nectin1	C	T	9: 43,803,498	H50Y	probably damaging	Het
Olfr1256	G	A	2: 89,835,234	T237I	possibly damaging	Het
Olfr516	T	C	7: 108,845,699	I104V	probably benign	Het
Olfr709-ps1	C	T	7: 106,926,569	V297M	possibly damaging	Het
Pfkfb2	G	A	1: 130,701,790	T303M	probably damaging	Het
Pkhd1l1	A	G	15: 44,505,309	D841G	probably benign	Het
Plxna4	A	T	6: 32,224,159		probably null	Het
Podnl1	C	T	8: 84,130,643	H294Y	probably benign	Het
Ppig	T	A	2: 69,749,650	D509E	unknown	Het
Ppp1r16a	T	A	15: 76,694,396	Y433*	probably null	Het
Prdm1	A	C	10: 44,450,225	V134G	possibly damaging	Het
Prx	T	C	7: 27,518,476	S940P	probably damaging	Het
Ptprd	C	T	4: 76,012,102	V731I	probably damaging	Het
Qk	A	G	17: 10,238,891		probably null	Het
Rassf6	T	C	5: 90,606,840	K206R	possibly damaging	Het
Rdh16f2	G	A	10: 127,876,255	E194K	possibly damaging	Het
Rims1	C	A	1: 22,483,247	G457*	probably null	Het
Ripk1	T	G	13: 34,013,296	I123R	probably damaging	Het
Rnf111	A	T	9: 70,476,140	S170R	probably benign	Het
Shc3	A	T	13: 51,461,377	F181L	probably damaging	Het
Siglec1	A	C	2: 131,085,577	V103G	probably damaging	Het
Slc26a11	G	T	11: 119,377,259	A488S	possibly damaging	Het
Smoc2	A	T	17: 14,336,579	T97S	probably damaging	Het
Son	T	C	16: 91,655,022	I219T	possibly damaging	Het
Sptbn1	A	T	11: 30,121,510	I1474N	possibly damaging	Het
Syt16	A	T	12: 74,222,768	D127V	possibly damaging	Het
Tet1	G	T	10: 62,878,578	N479K	possibly damaging	Het
Thsd7a	G	A	6: 12,338,655	A1192V	probably benign	Het
Tmed3	G	A	9: 89,699,772	R213*	probably null	Het
Traj57	A	G	14: 54,158,559		probably benign	Het
Trpv1	G	T	11: 73,238,516	R86S	probably benign	Het
Usp48	T	A	4: 137,616,362	F434L	possibly damaging	Het
Vmn2r59	A	C	7: 42,042,410		probably null	Het
Vmn2r71	T	A	7: 85,619,222	I211N	possibly damaging	Het
Vps13b	T	A	15: 35,422,453	D186E	probably damaging	Het
Vps33a	A	G	5: 123,558,628	S321P	probably damaging	Het
Wdr12	A	T	1: 60,094,511	D19E	probably benign	Het
Zc3h12c	A	G	9: 52,126,647	F278L	probably damaging	Het
Zpld1	T	C	16: 55,246,644	T183A	probably damaging	Het
Zswim2	G	T	2: 83,939,666	T68K	possibly damaging	Het

Other mutations in Olfr804

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01613:Olfr804	APN	10	129705623	missense	probably benign	0.01
IGL02041:Olfr804	APN	10	129705235	missense	probably damaging	1.00
IGL02245:Olfr804	APN	10	129705739	missense	probably damaging	1.00
IGL02341:Olfr804	APN	10	129705489	missense	probably damaging	0.99
IGL02433:Olfr804	APN	10	129705576	missense	probably benign	0.01
authen	UTSW	10	129705799	missense	probably benign	0.00
R0111:Olfr804	UTSW	10	129705277	missense	probably damaging	1.00
R0309:Olfr804	UTSW	10	129705139	missense	probably benign	0.38
R0326:Olfr804	UTSW	10	129705769	missense	possibly damaging	0.69
R0374:Olfr804	UTSW	10	129705647	missense	probably benign	0.00
R1573:Olfr804	UTSW	10	129705618	missense	probably damaging	1.00
R1663:Olfr804	UTSW	10	129705291	missense	probably benign	0.44
R1778:Olfr804	UTSW	10	129705705	missense	probably benign	0.01
R1789:Olfr804	UTSW	10	129705607	missense	possibly damaging	0.82
R1906:Olfr804	UTSW	10	129705496	missense	probably benign	0.00
R2108:Olfr804	UTSW	10	129705621	missense	probably benign
R2211:Olfr804	UTSW	10	129705451	missense	probably benign
R2432:Olfr804	UTSW	10	129704925	missense	possibly damaging	0.91
R2902:Olfr804	UTSW	10	129705451	missense	probably benign
R4114:Olfr804	UTSW	10	129705799	missense	probably benign	0.00
R5149:Olfr804	UTSW	10	129705508	missense	probably benign	0.00
R5846:Olfr804	UTSW	10	129704887	missense	probably damaging	0.99
R6553:Olfr804	UTSW	10	129705063	missense	probably benign	0.07
R7676:Olfr804	UTSW	10	129705286	missense	possibly damaging	0.63
R8161:Olfr804	UTSW	10	129704884	missense	possibly damaging	0.94
R9266:Olfr804	UTSW	10	129705678	missense	probably benign	0.17
R9318:Olfr804	UTSW	10	129705414	missense	probably benign	0.00
R9334:Olfr804	UTSW	10	129705814	missense	probably benign	0.00
R9746:Olfr804	UTSW	10	129705339	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- CAATTACATGATGAGCTTGGTGGG -3'
(R):5'- ATCCAGCTTCAGACCCATGG -3'

Sequencing Primer
(F):5'- GGGAACTTAATCATTGTGCTTCTCAC -3'
(R):5'- GCTTCAGACCCATGGCCAATG -3'

Posted On

2016-06-21