|Institutional Source||Beutler Lab|
|Gene Name||coagulation factor II|
|Synonyms||FII, Cf2, Cf-2, thrombin, prothrombin|
|Essential gene?||Essential (E-score: 1.000)|
|Stock #||R5095 (G1)|
|Chromosomal Location||91625320-91636414 bp(-) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||CAGAAAG to CAG at 91634957 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000106967 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028681] [ENSMUST00000111335]|
|Coding Region Coverage||
|Validation Efficiency||98% (59/60)|
FUNCTION: This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day. [provided by RefSeq, Apr 2015]
PHENOTYPE: Homozygotes for targeted null mutations exhibit defects in yolk sac vasculature, internal bleeding, tissue necrosis, and die in mid- to late-gestation, or rarely, a few days after birth. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in F2||
(F):5'- CAACAGTCTAAGTGCCAGCC -3'
(R):5'- CGCTCCACTAGAGAATGTCCTC -3'
(F):5'- GGCCACATGCAAAGACATCCTTG -3'
(R):5'- AGAGAATGTCCTCTCCCTCAG -3'