Mutagenetix > Incidental Mutation

Incidental Mutation 'R5134:Oprl1'

396015

Institutional Source

Beutler Lab

Gene Symbol

Oprl1

Ensembl Gene

ENSMUSG00000027584

Gene Name

opioid receptor-like 1

Synonyms

MOR-C, morc, nociceptin/ orphaninFQ receptor, NOP, LC132, N/OFQ receptor, ORL1, XOR1

MMRRC Submission

042722-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.383) question?

Stock #

R5134 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

181356809-181362778 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 181360403 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 153 (I153N)

Ref Sequence

ENSEMBL: ENSMUSP00000104399 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071585] [ENSMUST00000108763] [ENSMUST00000108766] [ENSMUST00000108767] [ENSMUST00000108768] [ENSMUST00000148334] [ENSMUST00000184795] [ENSMUST00000183693] [ENSMUST00000184127]

AlphaFold

P35377

Predicted Effect

probably damaging
Transcript: ENSMUST00000071585
AA Change: I153N

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000071513
Gene: ENSMUSG00000027584
AA Change: I153N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	54	334	5.8e-9	PFAM
Pfam:7TM_GPCR_Srx	56	264	1.4e-6	PFAM
Pfam:7TM_GPCR_Srsx	59	331	1.2e-14	PFAM
Pfam:7tm_1	65	316	1.4e-62	PFAM
Pfam:7TM_GPCR_Srv	112	332	7.8e-12	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000108763
AA Change: I139N

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000104394
Gene: ENSMUSG00000027584
AA Change: I139N

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	59	317	6.9e-11	PFAM
Pfam:7tm_1	60	302	1.6e-62	PFAM
Pfam:7TM_GPCR_Srv	91	318	4.3e-12	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000108766
AA Change: I153N

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000104397
Gene: ENSMUSG00000027584
AA Change: I153N

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	56	189	3.6e-8	PFAM
Pfam:7TM_GPCR_Srsx	59	201	1.8e-9	PFAM
Pfam:7tm_1	65	210	1.6e-41	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000108767
AA Change: I153N

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000104398
Gene: ENSMUSG00000027584
AA Change: I153N

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	59	331	1.2e-14	PFAM
Pfam:7tm_1	65	316	2.1e-66	PFAM
Pfam:7TM_GPCR_Srv	105	332	7.6e-12	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000108768
AA Change: I153N

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000104399
Gene: ENSMUSG00000027584
AA Change: I153N

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	59	331	1.2e-14	PFAM
Pfam:7tm_1	65	316	2.1e-66	PFAM
Pfam:7TM_GPCR_Srv	105	332	7.6e-12	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126835

Predicted Effect

probably benign
Transcript: ENSMUST00000148334

SMART Domains

Protein: ENSMUSP00000118664
Gene: ENSMUSG00000027584

Domain	Start	End	E-Value	Type
Pfam:7tm_1	65	140	5.9e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151241

Predicted Effect

probably benign
Transcript: ENSMUST00000184795

SMART Domains

Protein: ENSMUSP00000138979
Gene: ENSMUSG00000027584

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	21	71	2e-3	SMART
PDB:4EA3\|B	41	71	5e-13	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000183693

SMART Domains

Protein: ENSMUSP00000138810
Gene: ENSMUSG00000027584

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	21	77	1e-3	SMART
PDB:4EA3\|B	41	76	9e-17	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000184127

SMART Domains

Protein: ENSMUSP00000139119
Gene: ENSMUSG00000027584

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	21	76	3e-4	SMART
PDB:4EA3\|B	41	76	1e-16	PDB

Meta Mutation Damage Score

0.4108

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.5%

Validation Efficiency

96% (100/104)

MGI Phenotype

FUNCTION: The protein encoded by this gene is a member of the 7 transmembrane-spanning G protein-coupled receptor family, and functions as a receptor for the endogenous, opioid-related neuropeptide, nociceptin/orphanin FQ. This receptor-ligand system modulates a variety of biological functions and neurobehavior, including stress responses and anxiety behavior, learning and memory, locomotor activity, and inflammatory and immune responses. Alternatively spliced transcript variants have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mice homozygous for a null allele exhibit altered touch/nociception, facilitation of long-term potentiation and memory, increased dopamine release upon administration of opioid peptide agonist, impaired behavioral response to morphine, and increased susceptibility to noise-induced hearing loss. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adarb1	T	A	10: 77,161,679 (GRCm39)		probably benign	Het
Adrb3	A	C	8: 27,717,798 (GRCm39)	M217R	probably damaging	Het
Ank2	A	T	3: 126,757,094 (GRCm39)	N1054K	possibly damaging	Het
Aox4	A	T	1: 58,275,835 (GRCm39)	D389V	probably benign	Het
Aph1a	G	T	3: 95,802,843 (GRCm39)	G148W	probably damaging	Het
Arsj	A	G	3: 126,231,803 (GRCm39)	D183G	probably benign	Het
Atg2b	A	G	12: 105,641,209 (GRCm39)	S76P	probably damaging	Het
Atxn7l1	C	A	12: 33,422,875 (GRCm39)	N815K	probably damaging	Het
Cd3d	A	T	9: 44,896,296 (GRCm39)	E28D	probably damaging	Het
Cir1	G	A	2: 73,114,847 (GRCm39)	R404*	probably null	Het
Cops8	A	G	1: 90,538,724 (GRCm39)	D51G	probably damaging	Het
Csgalnact1	T	C	8: 68,913,623 (GRCm39)	E194G	probably benign	Het
Cstf2t	T	A	19: 31,061,494 (GRCm39)	D343E	probably damaging	Het
Ddx23	A	T	15: 98,548,651 (GRCm39)	D352E	possibly damaging	Het
Dhx34	G	T	7: 15,952,175 (GRCm39)	A150D	possibly damaging	Het
Dnal4	A	T	15: 79,647,766 (GRCm39)	V32E	possibly damaging	Het
Dvl3	G	T	16: 20,343,357 (GRCm39)		probably benign	Het
Ehmt1	G	T	2: 24,767,509 (GRCm39)	P135T	probably damaging	Het
Enpp2	C	T	15: 54,762,726 (GRCm39)	R174H	probably damaging	Het
Eps15	G	A	4: 109,223,727 (GRCm39)		probably benign	Het
F10	T	C	8: 13,105,698 (GRCm39)	V421A	probably damaging	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fer1l6	A	T	15: 58,512,003 (GRCm39)	D1490V	probably damaging	Het
Fsd1l	A	G	4: 53,647,766 (GRCm39)	K70E	probably benign	Het
Galnt12	G	A	4: 47,113,818 (GRCm39)	A79T	probably damaging	Het
Galnt17	A	T	5: 130,992,873 (GRCm39)	M347K	probably damaging	Het
Glyatl3	A	G	17: 41,215,921 (GRCm39)	V195A	probably benign	Het
Gm10770	A	T	2: 150,021,480 (GRCm39)		probably null	Het
Gm5578	A	T	6: 112,583,046 (GRCm39)		noncoding transcript	Het
Grn	C	A	11: 102,321,380 (GRCm39)		probably benign	Het
Gsk3b	G	A	16: 38,060,882 (GRCm39)	R418H	probably damaging	Het
Hspa12b	A	G	2: 130,981,428 (GRCm39)	E221G	possibly damaging	Het
Ighv1-50	T	G	12: 115,083,653 (GRCm39)	Q22H	probably benign	Het
Ipcef1	A	G	10: 6,869,950 (GRCm39)	I150T	probably benign	Het
Ipp	T	A	4: 116,372,654 (GRCm39)	F228I	possibly damaging	Het
Itgb7	A	G	15: 102,125,842 (GRCm39)	C596R	probably damaging	Het
Kif27	T	C	13: 58,438,904 (GRCm39)	D1219G	possibly damaging	Het
Lats1	T	A	10: 7,567,575 (GRCm39)	D115E	probably benign	Het
Lpcat3	A	G	6: 124,679,493 (GRCm39)	N331S	probably benign	Het
Lrpprc	A	T	17: 85,058,684 (GRCm39)	N725K	probably benign	Het
Lss	T	G	10: 76,382,070 (GRCm39)		probably benign	Het
Mfsd6	T	C	1: 52,747,515 (GRCm39)	Y450C	possibly damaging	Het
Mmp21	A	T	7: 133,280,742 (GRCm39)	V76E	probably benign	Het
Mtmr11	A	T	3: 96,077,223 (GRCm39)	Y527F	probably damaging	Het
Nat10	A	T	2: 103,573,638 (GRCm39)	F327I	probably benign	Het
Nfkbiz	A	T	16: 55,638,863 (GRCm39)	M199K	probably damaging	Het
Nuak1	A	G	10: 84,210,214 (GRCm39)	Y625H	probably benign	Het
Odf1	A	G	15: 38,226,393 (GRCm39)	T98A	possibly damaging	Het
Or10g3b	T	C	14: 52,587,248 (GRCm39)	N85S	probably benign	Het
Or1j4	A	T	2: 36,740,488 (GRCm39)	L143F	probably benign	Het
Or4x13	T	A	2: 90,231,107 (GRCm39)	M34K	probably benign	Het
Osbpl8	T	A	10: 111,124,554 (GRCm39)	D705E	probably benign	Het
Otud4	G	A	8: 80,382,318 (GRCm39)	V176I	probably damaging	Het
Paics	T	C	5: 77,104,669 (GRCm39)		probably benign	Het
Pax5	A	G	4: 44,710,407 (GRCm39)	M1T	probably null	Het
Pck1	A	T	2: 172,995,282 (GRCm39)	N34I	probably benign	Het
Pde8b	T	C	13: 95,223,250 (GRCm39)	I335V	probably benign	Het
Piezo2	A	G	18: 63,207,691 (GRCm39)	V1440A	probably damaging	Het
Prr23a4	T	C	9: 98,785,793 (GRCm39)	S153P	probably benign	Het
Ptpre	T	A	7: 135,253,821 (GRCm39)	F83Y	probably damaging	Het
Pwwp3a	T	C	10: 80,068,702 (GRCm39)	L282P	probably benign	Het
Rassf6	T	A	5: 90,752,225 (GRCm39)		probably null	Het
Rilp	A	T	11: 75,403,534 (GRCm39)	L325F	probably damaging	Het
Rpf1	A	G	3: 146,212,293 (GRCm39)	L349S	probably damaging	Het
Rprd2	G	C	3: 95,672,632 (GRCm39)	R924G	probably benign	Het
Rps6kc1	T	A	1: 190,505,845 (GRCm39)	D1039V	probably damaging	Het
Rufy3	G	A	5: 88,793,426 (GRCm39)	V579I	probably benign	Het
Serpinc1	A	G	1: 160,825,140 (GRCm39)		probably null	Het
Siae	T	G	9: 37,557,816 (GRCm39)	I541S	possibly damaging	Het
St14	T	G	9: 31,006,879 (GRCm39)	D649A	probably benign	Het
Stab2	A	G	10: 86,707,674 (GRCm39)		probably null	Het
Sun3	T	C	11: 8,988,287 (GRCm39)	T12A	probably benign	Het
Tdrd6	A	G	17: 43,937,101 (GRCm39)	Y1316H	probably damaging	Het
Thbs3	CAGAAG	CAG	3: 89,130,409 (GRCm39)		probably benign	Het
Tmem161b	C	A	13: 84,442,887 (GRCm39)	T269K	possibly damaging	Het
Trav14-3	T	A	14: 54,000,701 (GRCm39)	S17T	unknown	Het
Trmu	T	A	15: 85,780,556 (GRCm39)		probably null	Het
Ttn	T	A	2: 76,537,586 (GRCm39)	T26454S	possibly damaging	Het
Ubr3	T	C	2: 69,850,790 (GRCm39)		probably benign	Het
Unc5b	A	T	10: 60,610,879 (GRCm39)	V376E	probably benign	Het
Usp3	T	C	9: 66,449,814 (GRCm39)	S166G	possibly damaging	Het
Vldlr	C	A	19: 27,216,212 (GRCm39)	C344*	probably null	Het
Vmn1r17	A	G	6: 57,337,828 (GRCm39)	V130A	probably benign	Het
Wdfy3	A	C	5: 102,091,969 (GRCm39)	Y457D	probably damaging	Het
Zbtb2	T	C	10: 4,319,267 (GRCm39)	Y253C	possibly damaging	Het
Zfp687	A	C	3: 94,917,697 (GRCm39)	F692V	probably damaging	Het
Zfp758	A	G	17: 22,594,386 (GRCm39)	K291E	probably damaging	Het
Zfp799	A	T	17: 33,039,415 (GRCm39)	C284S	probably damaging	Het
Zp1	C	A	19: 10,897,926 (GRCm39)	C5F	probably benign	Het

Other mutations in Oprl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02977:Oprl1	APN	2	181,360,304 (GRCm39)	missense	probably damaging	1.00
R0302:Oprl1	UTSW	2	181,361,021 (GRCm39)	missense	probably benign	0.01
R0453:Oprl1	UTSW	2	181,360,527 (GRCm39)	critical splice donor site	probably null
R1564:Oprl1	UTSW	2	181,360,733 (GRCm39)	missense	possibly damaging	0.79
R1618:Oprl1	UTSW	2	181,360,646 (GRCm39)	missense	probably benign	0.22
R4801:Oprl1	UTSW	2	181,361,046 (GRCm39)	missense	probably benign
R4802:Oprl1	UTSW	2	181,361,046 (GRCm39)	missense	probably benign
R5032:Oprl1	UTSW	2	181,360,795 (GRCm39)	missense	probably damaging	1.00
R5133:Oprl1	UTSW	2	181,360,403 (GRCm39)	missense	probably damaging	0.99
R6284:Oprl1	UTSW	2	181,359,784 (GRCm39)	intron	probably benign
R6374:Oprl1	UTSW	2	181,357,721 (GRCm39)	missense	probably damaging	1.00
R6394:Oprl1	UTSW	2	181,360,795 (GRCm39)	missense	probably damaging	1.00
R6843:Oprl1	UTSW	2	181,357,547 (GRCm39)	missense	probably damaging	1.00
R7009:Oprl1	UTSW	2	181,360,174 (GRCm39)	missense	probably damaging	1.00
R8329:Oprl1	UTSW	2	181,360,717 (GRCm39)	missense	probably damaging	1.00
R9241:Oprl1	UTSW	2	181,360,405 (GRCm39)	missense	probably damaging	1.00
R9452:Oprl1	UTSW	2	181,360,454 (GRCm39)	missense	possibly damaging	0.89
R9712:Oprl1	UTSW	2	181,360,212 (GRCm39)	missense	probably damaging	1.00
X0022:Oprl1	UTSW	2	181,357,600 (GRCm39)	missense	probably benign	0.28
X0024:Oprl1	UTSW	2	181,360,341 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCCAAATGGAGCGTTTCTTC -3'
(R):5'- AACCATGGGGAACTGATTGG -3'

Sequencing Primer
(F):5'- AATCTGGCACTGGCTGATAC -3'
(R):5'- CTGATTGGTCAGGGAGGAGGAC -3'

Posted On

2016-06-21