|Institutional Source||Beutler Lab|
|Gene Name||cryptochrome 1 (photolyase-like)|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R5162 (G1)|
|Chromosomal Location||85131700-85185064 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 85133286 bp|
|Amino Acid Change||Histidine to Leucine at position 558 (H558L)|
|Ref Sequence||ENSEMBL: ENSMUSP00000020227 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000020227]|
Crystal Structure of Mouse Cryptochrome1 in Complex with Period2 [X-RAY DIFFRACTION]
Crystal structure of mouse Cryptochrome 1 [X-RAY DIFFRACTION]
|Predicted Effect||probably benign
AA Change: H558L
PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
AA Change: H558L
|Meta Mutation Damage Score||0.0622|
|Coding Region Coverage||
|Validation Efficiency||98% (61/62)|
FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of this gene results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour shorter circadian period under constant darkness and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cry1||
(F):5'- TAGGACGCTTCCCACTGCTG -3'
(R):5'- TACATGTAAACACTGCACTCAAAGT -3'
(F):5'- TTCCCACTGCTGAGGCC -3'
(R):5'- AGACAACCAGTGCTTTATTTTTGCC -3'