Mutagenetix > Incidental Mutations

Incidental Mutation 'R5166:Zfp850'

397299

Institutional Source

Beutler Lab

Gene Symbol

Zfp850

Ensembl Gene

ENSMUSG00000096916

Gene Name

zinc finger protein 850

Synonyms

C130069I09Rik, Gm4636

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R5166 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

27984854-28014115 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 27990356 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 142 (C142*)

Ref Sequence

ENSEMBL: ENSMUSP00000141063 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099111] [ENSMUST00000180024] [ENSMUST00000180502]

Predicted Effect

probably benign
Transcript: ENSMUST00000099111

Predicted Effect

probably null
Transcript: ENSMUST00000180024
AA Change: C142*

SMART Domains

Protein: ENSMUSP00000137192
Gene: ENSMUSG00000096916
AA Change: C142*

Domain	Start	End	E-Value	Type
KRAB	14	75	1.56e-34	SMART
ZnF_C2H2	172	194	7.18e1	SMART
ZnF_C2H2	200	222	3.63e-3	SMART
ZnF_C2H2	228	250	8.94e-3	SMART
ZnF_C2H2	256	278	7.49e-5	SMART
ZnF_C2H2	313	335	1.01e-1	SMART
ZnF_C2H2	341	363	4.4e-2	SMART
ZnF_C2H2	369	391	7.37e-4	SMART
ZnF_C2H2	397	419	8.47e-4	SMART
ZnF_C2H2	425	447	1.92e-2	SMART
ZnF_C2H2	453	475	2.99e-4	SMART
ZnF_C2H2	481	503	7.78e-3	SMART
ZnF_C2H2	509	531	1.95e-3	SMART
ZnF_C2H2	537	559	1.92e-2	SMART
ZnF_C2H2	565	587	2.99e-4	SMART
ZnF_C2H2	593	615	1.79e-2	SMART
ZnF_C2H2	621	643	7.37e-4	SMART
ZnF_C2H2	649	671	4.4e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000180502
AA Change: C142*

SMART Domains

Protein: ENSMUSP00000141063
Gene: ENSMUSG00000096916
AA Change: C142*

Domain	Start	End	E-Value	Type
KRAB	14	75	6.5e-37	SMART
ZnF_C2H2	172	194	3e-1	SMART
ZnF_C2H2	200	222	1.5e-5	SMART
ZnF_C2H2	228	250	3.8e-5	SMART
ZnF_C2H2	256	274	2.5e-1	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad12	T	C	5: 121,600,020	T435A	probably benign	Het
Adcy3	T	A	12: 4,134,438	I38N	probably damaging	Het
Aff1	A	G	5: 103,754,657		probably benign	Het
Asxl1	A	G	2: 153,401,121	E1197G	probably damaging	Het
Brinp3	T	A	1: 146,901,367	N517K	probably damaging	Het
Carmil1	C	T	13: 24,154,983		probably null	Het
Ccdc185	G	A	1: 182,748,999	Q42*	probably null	Het
Cdc23	C	A	18: 34,651,689	V7L	unknown	Het
Cdh10	A	G	15: 19,013,360	E682G	probably damaging	Het
Col6a3	C	A	1: 90,810,608	R456L	probably damaging	Het
Egf	C	T	3: 129,735,840	R307H	probably benign	Het
Flt4	T	C	11: 49,633,257		probably null	Het
Fstl5	A	C	3: 76,628,960	K26Q	possibly damaging	Het
Gk5	G	T	9: 96,174,768	A413S	probably damaging	Het
Glcci1	T	A	6: 8,537,854	S157R	probably benign	Het
Gzmc	G	A	14: 56,233,976	A36V	probably damaging	Het
Hexb	T	C	13: 97,182,004	N283S	probably benign	Het
Hydin	A	G	8: 110,523,142	E2239G	possibly damaging	Het
Igkv10-95	A	T	6: 68,680,560	Y20F	probably benign	Het
Il17re	A	G	6: 113,462,962	T181A	probably benign	Het
Kcnh6	G	A	11: 106,020,319	A514T	possibly damaging	Het
Kcnk10	G	A	12: 98,434,995	R460W	probably damaging	Het
Krtap5-2	A	T	7: 142,174,984	S320T	unknown	Het
Larp1b	G	T	3: 40,964,052	E24*	probably null	Het
Mettl22	A	G	16: 8,478,251	T135A	probably benign	Het
Mlh1	A	G	9: 111,241,513	V378A	probably benign	Het
Mmrn1	A	T	6: 60,976,490	H585L	probably benign	Het
Myh14	A	G	7: 44,628,855	F1024L	probably damaging	Het
Nbea	A	T	3: 56,019,453	H776Q	probably damaging	Het
Nod2	A	G	8: 88,664,247	D372G	possibly damaging	Het
Nptn	C	T	9: 58,618,980	R137*	probably null	Het
Olfr825	T	C	10: 130,162,561	Y255C	possibly damaging	Het
Pm20d2	C	T	4: 33,181,803	V267I	probably benign	Het
Rassf10	A	G	7: 112,954,420	D76G	probably benign	Het
Rbbp5	A	G	1: 132,490,565	T41A	possibly damaging	Het
Rttn	T	C	18: 89,013,094	V643A	possibly damaging	Het
Sbno2	C	A	10: 80,066,928	E421*	probably null	Het
Slc17a3	T	C	13: 23,842,542		probably null	Het
Slc1a6	T	A	10: 78,796,269		probably null	Het
Slco2b1	A	G	7: 99,689,013	S106P	possibly damaging	Het
Spef1	T	C	2: 131,174,591	N28S	probably damaging	Het
Srebf2	C	A	15: 82,185,402	T675N	probably damaging	Het
Srp68	C	A	11: 116,265,474	E147D	probably damaging	Het
Tbcd	T	A	11: 121,609,390	L1114H	possibly damaging	Het
Tex15	T	C	8: 33,576,392	V1950A	probably benign	Het
Tnfsf9	T	A	17: 57,106,263	F148Y	possibly damaging	Het
Traf6	A	G	2: 101,690,057	D150G	probably benign	Het
Ttn	A	G	2: 76,863,373	Y262H	possibly damaging	Het
Unc93b1	A	G	19: 3,944,027	Y386C	probably damaging	Het
Vmn1r226	A	T	17: 20,687,863	E119V	probably benign	Het
Wrn	A	T	8: 33,352,072		probably null	Het
Zfp398	A	G	6: 47,865,904	I165V	probably benign	Het

Other mutations in Zfp850

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02278:Zfp850	APN	7	28008397	missense	probably damaging	0.96
R0610:Zfp850	UTSW	7	27989394	missense	probably damaging	0.99
R0690:Zfp850	UTSW	7	27985217	missense	possibly damaging	0.67
R0711:Zfp850	UTSW	7	27990273	missense	probably benign	0.00
R1310:Zfp850	UTSW	7	27989459	missense	probably benign	0.40
R1594:Zfp850	UTSW	7	27989391	missense	probably benign	0.00
R1771:Zfp850	UTSW	7	27985275	nonsense	probably null
R2189:Zfp850	UTSW	7	27989055	missense	probably benign	0.02
R2192:Zfp850	UTSW	7	27985195	missense	probably damaging	1.00
R2417:Zfp850	UTSW	7	27989183	missense	possibly damaging	0.54
R4321:Zfp850	UTSW	7	27989400	missense	probably damaging	0.99
R4770:Zfp850	UTSW	7	27984986	splice site	probably null
R4970:Zfp850	UTSW	7	27990233	nonsense	probably null
R5112:Zfp850	UTSW	7	27990233	nonsense	probably null
R5303:Zfp850	UTSW	7	28008413	missense	probably damaging	1.00
R5315:Zfp850	UTSW	7	27990318	missense	probably benign	0.02
R5496:Zfp850	UTSW	7	28007346	missense	probably damaging	0.98
R5547:Zfp850	UTSW	7	27989419	missense	probably damaging	1.00
R5677:Zfp850	UTSW	7	27989088	missense	probably damaging	1.00
R5927:Zfp850	UTSW	7	27990195	missense	probably benign	0.17
R6654:Zfp850	UTSW	7	27985215	nonsense	probably null
R6950:Zfp850	UTSW	7	27990514	missense	possibly damaging	0.86
R6987:Zfp850	UTSW	7	27990001	missense	probably damaging	1.00
R6990:Zfp850	UTSW	7	27990376	missense	probably benign	0.09
R7640:Zfp850	UTSW	7	27989209	missense	probably benign	0.05
R7856:Zfp850	UTSW	7	27990474	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- TGCTAGAACAGTGAAGGCAC -3'
(R):5'- TCTCGGAAAATGCTTTAGAGTCAG -3'

Sequencing Primer
(F):5'- CTGCCACATTCTTTACACTGAAAGGG -3'
(R):5'- AATGCTTTAGAGTCAGGCTATTTTG -3'

Posted On

2016-07-06