Mutagenetix > Incidental Mutation

Incidental Mutation 'R5168:Gpr31b'

397412

Institutional Source

Beutler Lab

Gene Symbol

Gpr31b

Ensembl Gene

ENSMUSG00000071311

Gene Name

G protein-coupled receptor 31, D17Leh66b region

Synonyms

MMRRC Submission

042748-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R5168 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

13270208-13271167 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 13270326 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 281 (I281N)

Ref Sequence

ENSEMBL: ENSMUSP00000089237 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091648]

AlphaFold

F8VQN3

Predicted Effect

probably damaging
Transcript: ENSMUST00000091648
AA Change: I281N

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000089237
Gene: ENSMUSG00000071311
AA Change: I281N

Domain	Start	End	E-Value	Type
Pfam:7tm_1	31	282	3.9e-30	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

96% (47/49)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca1	T	C	4: 53,086,070 (GRCm39)	N478D	probably benign	Het
Acsl1	T	A	8: 46,966,303 (GRCm39)		probably benign	Het
Aox1	T	A	1: 58,088,561 (GRCm39)	C116S	probably damaging	Het
Bag6	T	C	17: 35,363,671 (GRCm39)	L785P	probably damaging	Het
Calcr	T	C	6: 3,708,610 (GRCm39)	N192S	probably benign	Het
Cntrl	T	A	2: 35,047,667 (GRCm39)	L1414H	probably damaging	Het
Cntrob	T	A	11: 69,190,816 (GRCm39)	I849F	possibly damaging	Het
Col6a3	C	T	1: 90,701,361 (GRCm39)	W2518*	probably null	Het
Cxcl15	T	A	5: 90,943,142 (GRCm39)	I48K	probably damaging	Het
Dab2	T	C	15: 6,365,924 (GRCm39)		probably benign	Het
Ddx54	A	G	5: 120,755,097 (GRCm39)	E82G	probably benign	Het
Dock1	T	C	7: 134,720,637 (GRCm39)	W1249R	probably damaging	Het
Fras1	A	C	5: 96,856,616 (GRCm39)	M2000L	probably benign	Het
Gvin3	T	C	7: 106,196,054 (GRCm39)		noncoding transcript	Het
Haus5	T	C	7: 30,357,136 (GRCm39)	T432A	possibly damaging	Het
Hecw2	A	G	1: 53,952,459 (GRCm39)	S925P	probably damaging	Het
Katnal1	A	G	5: 148,858,132 (GRCm39)	M26T	possibly damaging	Het
Mccc1	C	T	3: 36,044,929 (GRCm39)	W71*	probably null	Het
Muc6	G	A	7: 141,223,981 (GRCm39)		probably benign	Het
Nrbp1	T	A	5: 31,407,481 (GRCm39)	V397D	probably damaging	Het
Nt5dc1	T	A	10: 34,273,236 (GRCm39)	E187D	probably benign	Het
Or1e19	T	A	11: 73,316,669 (GRCm39)	I47F	probably benign	Het
Or51f23	C	T	7: 102,453,528 (GRCm39)	A281V	probably benign	Het
Polr1c	G	T	17: 46,558,635 (GRCm39)		probably benign	Het
Pramel27	T	C	4: 143,579,768 (GRCm39)	V451A	probably benign	Het
Ralgapa1	A	G	12: 55,804,817 (GRCm39)	V493A	probably benign	Het
Ryr2	T	C	13: 11,767,207 (GRCm39)	T1228A	probably benign	Het
Slc26a3	G	A	12: 31,518,553 (GRCm39)	V674I	possibly damaging	Het
Spata31f1a	C	T	4: 42,851,488 (GRCm39)	V223I	probably damaging	Het
Srp68	C	A	11: 116,156,300 (GRCm39)	E147D	probably damaging	Het
Tacr3	T	C	3: 134,535,320 (GRCm39)	I96T	probably damaging	Het
Tmem236	T	C	2: 14,197,139 (GRCm39)		probably null	Het
Tmem62	T	A	2: 120,824,088 (GRCm39)	N254K	probably benign	Het
Tmem79	A	T	3: 88,240,651 (GRCm39)	L99Q	probably damaging	Het
Trav6-5	A	T	14: 53,728,973 (GRCm39)	N78Y	probably benign	Het
Trim33	C	T	3: 103,248,997 (GRCm39)	Q807*	probably null	Het
Ugt1a10	A	G	1: 87,983,531 (GRCm39)	T110A	probably benign	Het
Vcl	C	T	14: 21,060,170 (GRCm39)	T603I	probably damaging	Het
Vps8	A	T	16: 21,276,195 (GRCm39)	T243S	probably damaging	Het
Vps8	A	C	16: 21,351,849 (GRCm39)	I323L	probably benign	Het
Zfp746	G	C	6: 48,041,329 (GRCm39)	Q465E	possibly damaging	Het

Other mutations in Gpr31b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0312:Gpr31b	UTSW	17	13,270,498 (GRCm39)	missense	probably damaging	0.99
R0645:Gpr31b	UTSW	17	13,271,093 (GRCm39)	nonsense	probably null
R5616:Gpr31b	UTSW	17	13,270,831 (GRCm39)	missense	probably damaging	1.00
R6145:Gpr31b	UTSW	17	13,270,266 (GRCm39)	missense	possibly damaging	0.87
R6927:Gpr31b	UTSW	17	13,270,765 (GRCm39)	missense	probably damaging	1.00
R7211:Gpr31b	UTSW	17	13,271,104 (GRCm39)	missense	probably benign	0.00
R7510:Gpr31b	UTSW	17	13,270,557 (GRCm39)	missense	probably damaging	1.00
R9366:Gpr31b	UTSW	17	13,270,375 (GRCm39)	missense	probably damaging	1.00
R9470:Gpr31b	UTSW	17	13,270,845 (GRCm39)	missense	probably damaging	1.00
R9602:Gpr31b	UTSW	17	13,270,771 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

PCR Primer
(F):5'- ACGTACTGTGTCTGGATTCG -3'
(R):5'- TGGTTGCCATAGTGCTGCTAC -3'

Sequencing Primer
(F):5'- CTCCTTATCCTGAGACTATTGGG -3'
(R):5'- CCATAGTGCTGCTACTGTTTGGAC -3'

Posted On

2016-07-06