Mutagenetix > Incidental Mutation

Incidental Mutation 'R5181:Odad4'

397560

Institutional Source

Beutler Lab

Gene Symbol

Odad4

Ensembl Gene

ENSMUSG00000006784

Gene Name

outer dynein arm complex subunit 4

Synonyms

4933404O19Rik, Ttc25

MMRRC Submission

042761-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R5181 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

100436434-100463392 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 100440719 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 67 (D67N)

Ref Sequence

ENSEMBL: ENSMUSP00000090355 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006976] [ENSMUST00000092684]

AlphaFold

Q9D4B2

Predicted Effect

probably damaging
Transcript: ENSMUST00000006976
AA Change: D67N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000006976
Gene: ENSMUSG00000006784
AA Change: D67N

Domain	Start	End	E-Value	Type
TPR	11	44	3.69e1	SMART
TPR	45	78	3.07e1	SMART
TPR	79	112	4.96e0	SMART
low complexity region	147	158	N/A	INTRINSIC
low complexity region	191	208	N/A	INTRINSIC
TPR	318	351	4.96e0	SMART
TPR	358	391	1.11e1	SMART
Blast:TPR	395	428	7e-14	BLAST
TPR	435	468	2.99e1	SMART
low complexity region	493	505	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000092684
AA Change: D67N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000090355
Gene: ENSMUSG00000006784
AA Change: D67N

Domain	Start	End	E-Value	Type
TPR	11	44	3.69e1	SMART
TPR	45	78	3.07e1	SMART
TPR	79	112	4.96e0	SMART
low complexity region	147	158	N/A	INTRINSIC
low complexity region	191	208	N/A	INTRINSIC
TPR	318	351	4.96e0	SMART
TPR	358	391	1.11e1	SMART
Blast:TPR	395	428	5e-14	BLAST
TPR	435	468	2.99e1	SMART
coiled coil region	528	548	N/A	INTRINSIC
low complexity region	556	567	N/A	INTRINSIC
coiled coil region	599	624	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127389

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132143

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150331

Meta Mutation Damage Score

0.6821

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

100% (54/54)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele display partial preweaning lethality, impaired ciliary motility, and a variety of left-right body symmetry defects. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1a	T	A	5: 8,764,937 (GRCm39)	D642E	probably benign	Het
Anxa2	T	C	9: 69,383,347 (GRCm39)	V54A	probably benign	Het
Ccnb1	C	G	13: 100,918,283 (GRCm39)	Q121H	possibly damaging	Het
Cdca2	A	G	14: 67,917,614 (GRCm39)	S595P	probably damaging	Het
Cenpe	G	A	3: 134,948,064 (GRCm39)	E1208K	probably damaging	Het
Cfh	T	C	1: 140,075,384 (GRCm39)		probably benign	Het
Colq	T	A	14: 31,279,799 (GRCm39)	H9L	probably benign	Het
Coq8b	T	C	7: 26,951,747 (GRCm39)	I403T	possibly damaging	Het
Cyrib	T	A	15: 63,810,526 (GRCm39)	M234L	probably damaging	Het
Dcdc2a	C	A	13: 25,386,347 (GRCm39)	T407K	possibly damaging	Het
Dennd2b	T	C	7: 109,155,997 (GRCm39)	Y251C	probably benign	Het
Gask1b	A	T	3: 79,793,618 (GRCm39)	S29C	probably benign	Het
Grhl3	T	A	4: 135,286,415 (GRCm39)	K89*	probably null	Het
Inpp5f	A	G	7: 128,281,555 (GRCm39)	T519A	probably damaging	Het
Isl2	G	T	9: 55,449,561 (GRCm39)	R79L	probably benign	Het
Kif9	T	A	9: 110,350,336 (GRCm39)	D742E	probably damaging	Het
Lgi2	T	A	5: 52,711,792 (GRCm39)	K176M	probably damaging	Het
Lin9	T	A	1: 180,496,763 (GRCm39)	L351I	probably benign	Het
Lrch4	A	G	5: 137,627,665 (GRCm39)	D66G	probably damaging	Het
Milr1	G	A	11: 106,645,806 (GRCm39)	G116D	probably damaging	Het
Myof	T	C	19: 37,921,071 (GRCm39)	D1397G	possibly damaging	Het
Neurod2	T	C	11: 98,218,204 (GRCm39)	H320R	probably benign	Het
Nox3	A	T	17: 3,685,561 (GRCm39)	Y562*	probably null	Het
Nrap	G	A	19: 56,333,960 (GRCm39)	H884Y	possibly damaging	Het
Pde3a	T	C	6: 141,426,981 (GRCm39)		probably null	Het
Pgm2l1	G	A	7: 99,910,965 (GRCm39)	C303Y	probably benign	Het
Phip	A	G	9: 82,753,243 (GRCm39)		probably benign	Het
Plxna4	A	G	6: 32,493,932 (GRCm39)	I228T	probably damaging	Het
Prdm2	A	G	4: 142,861,536 (GRCm39)	S585P	probably benign	Het
Prpf6	T	C	2: 181,291,339 (GRCm39)	I718T	probably damaging	Het
Rpp40	T	C	13: 36,080,695 (GRCm39)		probably null	Het
Skic2	T	C	17: 35,063,802 (GRCm39)	D547G	probably benign	Het
Slc22a22	T	A	15: 57,118,519 (GRCm39)	Y264F	probably benign	Het
Slc5a4b	A	T	10: 75,896,221 (GRCm39)	L578*	probably null	Het
Sptan1	A	T	2: 29,883,736 (GRCm39)		probably benign	Het
Sult2a4	T	G	7: 13,722,316 (GRCm39)	I50L	probably benign	Het
Taar6	T	C	10: 23,860,683 (GRCm39)	T288A	possibly damaging	Het
Tmem71	C	T	15: 66,427,063 (GRCm39)	S44N	probably benign	Het
Tmem98	T	C	11: 80,710,758 (GRCm39)	V139A	probably damaging	Het
Triobp	T	C	15: 78,851,954 (GRCm39)	Y703H	probably benign	Het
Ttc34	A	G	4: 154,946,703 (GRCm39)	T868A	probably benign	Het
Ttn	C	T	2: 76,665,225 (GRCm39)		probably benign	Het
Vipas39	A	T	12: 87,286,601 (GRCm39)	W470R	probably damaging	Het
Vmn2r102	T	C	17: 19,897,003 (GRCm39)	Y117H	probably benign	Het
Vmn2r111	T	C	17: 22,790,001 (GRCm39)	N335S	possibly damaging	Het
Vmn2r70	A	G	7: 85,208,387 (GRCm39)	Y697H	probably damaging	Het
Wnk4	C	A	11: 101,156,203 (GRCm39)	R461S	probably damaging	Het
Xylb	T	A	9: 119,193,567 (GRCm39)	L87Q	probably damaging	Het
Zcchc9	T	A	13: 91,945,281 (GRCm39)	K101*	probably null	Het
Zfp503	C	A	14: 22,035,705 (GRCm39)	A404S	probably benign	Het
Zhx1	C	G	15: 57,917,470 (GRCm39)	G259R	probably damaging	Het

Other mutations in Odad4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Odad4	APN	11	100,460,728 (GRCm39)	missense	probably damaging	1.00
IGL02379:Odad4	APN	11	100,457,809 (GRCm39)	missense	possibly damaging	0.87
IGL03091:Odad4	APN	11	100,441,076 (GRCm39)	nonsense	probably null
IGL02991:Odad4	UTSW	11	100,444,698 (GRCm39)	missense	probably benign	0.01
R0044:Odad4	UTSW	11	100,457,827 (GRCm39)	missense	probably damaging	1.00
R0137:Odad4	UTSW	11	100,454,394 (GRCm39)	missense	probably damaging	1.00
R0280:Odad4	UTSW	11	100,441,091 (GRCm39)	missense	probably damaging	1.00
R1373:Odad4	UTSW	11	100,436,658 (GRCm39)	missense	probably damaging	1.00
R1793:Odad4	UTSW	11	100,460,679 (GRCm39)	splice site	probably null
R2097:Odad4	UTSW	11	100,454,408 (GRCm39)	missense	possibly damaging	0.89
R2121:Odad4	UTSW	11	100,457,837 (GRCm39)	critical splice donor site	probably null
R2509:Odad4	UTSW	11	100,444,361 (GRCm39)	missense	probably damaging	0.99
R2655:Odad4	UTSW	11	100,444,405 (GRCm39)	missense	probably damaging	1.00
R4635:Odad4	UTSW	11	100,442,333 (GRCm39)	nonsense	probably null
R4773:Odad4	UTSW	11	100,440,742 (GRCm39)	missense	probably benign	0.05
R4858:Odad4	UTSW	11	100,441,147 (GRCm39)	missense	probably damaging	0.96
R5164:Odad4	UTSW	11	100,462,346 (GRCm39)	nonsense	probably null
R5707:Odad4	UTSW	11	100,444,887 (GRCm39)	missense	probably damaging	0.99
R5742:Odad4	UTSW	11	100,436,699 (GRCm39)	missense	possibly damaging	0.90
R7634:Odad4	UTSW	11	100,452,731 (GRCm39)	critical splice donor site	probably null
R7851:Odad4	UTSW	11	100,436,655 (GRCm39)	missense	probably damaging	1.00
R8186:Odad4	UTSW	11	100,454,505 (GRCm39)	missense	probably benign	0.27
R8194:Odad4	UTSW	11	100,454,502 (GRCm39)	missense	probably benign	0.06
R8444:Odad4	UTSW	11	100,452,731 (GRCm39)	critical splice donor site	probably null
R8879:Odad4	UTSW	11	100,457,752 (GRCm39)	nonsense	probably null
X0018:Odad4	UTSW	11	100,444,424 (GRCm39)	missense	probably damaging	0.99
X0028:Odad4	UTSW	11	100,436,724 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- AGGAGTCCATGTACTGACCTG -3'
(R):5'- CAGGTTGAAGGTCAAGGCTG -3'

Sequencing Primer
(F):5'- TCCATGTACTGACCTGGAGGG -3'
(R):5'- TCAAGGTTGGCCTGCGAGAG -3'

Posted On

2016-07-06