Mutagenetix > Incidental Mutation

Incidental Mutation 'R5182:Fras1'

397602

Institutional Source

Beutler Lab

Gene Symbol

Fras1

Ensembl Gene

ENSMUSG00000034687

Gene Name

Fraser extracellular matrix complex subunit 1

Synonyms

bl, E130113P14Rik

MMRRC Submission

042853-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5182 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

96373955-96784728 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 96636173 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 845 (C845*)

Ref Sequence

ENSEMBL: ENSMUSP00000043250 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036019]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000036019
AA Change: C845*

SMART Domains

Protein: ENSMUSP00000043250
Gene: ENSMUSG00000034687
AA Change: C845*

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWC	27	86	9.23e-9	SMART
VWC	94	151	9.37e-10	SMART
VWC	158	215	8.61e-9	SMART
VWC	220	277	7.1e-10	SMART
VWC	284	341	7.8e-7	SMART
VWC	365	415	1.93e-1	SMART
FU	408	459	3.33e-1	SMART
FU	461	504	9.12e-8	SMART
EGF_like	466	495	6.67e1	SMART
FU	506	552	6.01e-8	SMART
FU	554	598	2.21e-6	SMART
FU	601	646	1.28e-11	SMART
FU	648	704	4.19e-7	SMART
FU	707	752	9.12e-8	SMART
FU	754	799	1.11e-6	SMART
EGF_like	759	790	7.23e1	SMART
FU	802	851	5.44e-6	SMART
EGF_like	807	842	4.55e1	SMART
FU	853	899	7.4e-8	SMART
FU	902	947	4.78e-2	SMART
FU	951	996	4.52e-12	SMART
EGF_like	956	987	2.75e1	SMART
FU	998	1041	1.38e-7	SMART
FU	1045	1088	9.7e-3	SMART
EGF_like	1057	1096	3.16e1	SMART
Pfam:Cadherin_3	1098	1198	5.2e-12	PFAM
Pfam:Cadherin_3	1167	1309	6.5e-27	PFAM
Pfam:Cadherin_3	1278	1442	7e-24	PFAM
Pfam:Cadherin_3	1411	1560	1.3e-23	PFAM
Pfam:Cadherin_3	1561	1693	6.4e-15	PFAM
Pfam:Cadherin_3	1695	1814	1.1e-10	PFAM
Pfam:Cadherin_3	1780	1940	1.6e-18	PFAM
Pfam:Cadherin_3	1906	2061	2.8e-22	PFAM
Pfam:Cadherin_3	2063	2181	3.3e-18	PFAM
Pfam:Cadherin_3	2172	2295	1.4e-26	PFAM
Pfam:Cadherin_3	2296	2408	2.3e-31	PFAM
Pfam:Cadherin_3	2413	2540	7.9e-23	PFAM
Calx_beta	2544	2648	1.23e-10	SMART
Calx_beta	2661	2772	3.3e-11	SMART
Calx_beta	2787	2892	1.21e-9	SMART
Calx_beta	2907	3009	4.45e-3	SMART
Calx_beta	3027	3131	1.5e-14	SMART
Blast:Calx_beta	3162	3187	1e-5	BLAST
transmembrane domain	3902	3924	N/A	INTRINSIC
low complexity region	3927	3935	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197126

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000198016

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199204

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

100% (66/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Survival is variable on genetic backgrounds. Kidney development is severely affected and syndactyly is common. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059E24Rik	A	G	19: 21,630,765 (GRCm38)	M1T	probably null	Het
Abcg8	T	C	17: 84,692,744 (GRCm38)	L243P	probably damaging	Het
Ankrd27	A	G	7: 35,628,487 (GRCm38)	T811A	probably damaging	Het
Auts2	T	C	5: 131,475,081 (GRCm38)	N188S	probably null	Het
Bak1	G	A	17: 27,022,748 (GRCm38)	P65L	possibly damaging	Het
Cep350	A	C	1: 155,858,108 (GRCm38)	L3013R	probably damaging	Het
Clca3b	A	G	3: 144,828,015 (GRCm38)	I533T	probably damaging	Het
Col6a5	C	A	9: 105,857,332 (GRCm38)	E2637*	probably null	Het
Coq5	G	A	5: 115,279,756 (GRCm38)	R15H	probably benign	Het
Dnah5	T	G	15: 28,311,278 (GRCm38)	I1801S	probably damaging	Het
Dnhd1	G	A	7: 105,703,209 (GRCm38)	R2523Q	probably damaging	Het
Dpf3	T	C	12: 83,370,596 (GRCm38)	E34G	probably damaging	Het
Dst	C	A	1: 34,179,086 (GRCm38)	Q1536K	probably benign	Het
Eng	T	C	2: 32,672,959 (GRCm38)		probably null	Het
Fam102b	T	C	3: 108,985,351 (GRCm38)	K173E	possibly damaging	Het
Fignl1	A	T	11: 11,801,717 (GRCm38)	I446N	probably damaging	Het
Gfm2	T	C	13: 97,162,893 (GRCm38)	V347A	probably damaging	Het
Gpld1	A	T	13: 24,984,070 (GRCm38)		probably null	Het
Grn	C	A	11: 102,430,554 (GRCm38)		probably benign	Het
Gsdmc4	C	A	15: 63,893,804 (GRCm38)	V299F	probably damaging	Het
Hrnr	A	T	3: 93,332,143 (GRCm38)	R3229S	unknown	Het
Icam5	A	G	9: 21,034,810 (GRCm38)	T313A	probably benign	Het
Ift172	T	A	5: 31,267,614 (GRCm38)	D668V	possibly damaging	Het
Kdm5a	T	A	6: 120,388,105 (GRCm38)	C322*	probably null	Het
Klk6	A	G	7: 43,828,660 (GRCm38)	K152R	probably benign	Het
Man1a2	A	C	3: 100,647,017 (GRCm38)	I250S	probably damaging	Het
Nkpd1	A	G	7: 19,523,256 (GRCm38)	Y170C	probably damaging	Het
Nmd3	T	A	3: 69,722,468 (GRCm38)		probably null	Het
Olfr131	A	G	17: 38,082,114 (GRCm38)	M288T	probably benign	Het
Olfr566	A	T	7: 102,856,969 (GRCm38)	D104E	probably benign	Het
Pax4	C	T	6: 28,444,369 (GRCm38)	E229K	probably benign	Het
Pdc	T	C	1: 150,333,354 (GRCm38)	I196T	possibly damaging	Het
Pld1	T	C	3: 28,045,081 (GRCm38)	I299T	probably damaging	Het
Pnma1	T	C	12: 84,147,045 (GRCm38)	I295V	probably benign	Het
Prkg2	T	A	5: 99,024,709 (GRCm38)	Y49F	probably benign	Het
Psmd12	T	C	11: 107,479,659 (GRCm38)	L28P	probably damaging	Het
Ptprg	A	G	14: 12,154,174 (GRCm38)	T632A	probably benign	Het
Rab43	A	G	6: 87,794,655 (GRCm38)	*118R	probably null	Het
Rabep1	C	T	11: 70,904,628 (GRCm38)	R227*	probably null	Het
Rad51c	T	G	11: 87,397,719 (GRCm38)	I213L	possibly damaging	Het
Ranbp17	A	T	11: 33,219,287 (GRCm38)		probably benign	Het
Ryr3	T	C	2: 112,755,150 (GRCm38)	E2735G	probably damaging	Het
Sdc3	A	G	4: 130,821,684 (GRCm38)		probably benign	Het
Sirpa	G	A	2: 129,615,732 (GRCm38)	R242H	possibly damaging	Het
Slc12a4	G	A	8: 105,944,606 (GRCm38)	T983M	probably damaging	Het
Snx7	A	G	3: 117,832,857 (GRCm38)	Y252H	probably damaging	Het
St18	C	T	1: 6,817,653 (GRCm38)	T482I	probably benign	Het
St7	A	G	6: 17,846,237 (GRCm38)	Y163C	probably damaging	Het
Tas2r137	A	T	6: 40,491,932 (GRCm38)	Q232L	probably benign	Het
Tgm6	A	T	2: 130,141,302 (GRCm38)	K270N	probably damaging	Het
Tiam2	G	A	17: 3,438,721 (GRCm38)	G768D	probably damaging	Het
Ttn	C	T	2: 76,870,429 (GRCm38)		probably benign	Het
Ube4b	A	C	4: 149,381,242 (GRCm38)	S250R	probably null	Het
Ubxn2a	G	A	12: 4,880,634 (GRCm38)	A242V	probably damaging	Het
Usp10	G	A	8: 119,956,681 (GRCm38)	V764I	possibly damaging	Het
Vmn1r222	A	G	13: 23,232,497 (GRCm38)	L182P	probably damaging	Het
Vmn2r114	A	G	17: 23,291,658 (GRCm38)	V616A	probably damaging	Het
Vps13a	A	G	19: 16,695,499 (GRCm38)	V1303A	possibly damaging	Het
Wnk2	G	T	13: 49,061,161 (GRCm38)	T1315K	possibly damaging	Het
Zfp936	G	A	7: 43,189,907 (GRCm38)	C266Y	probably damaging	Het
Zscan20	G	T	4: 128,586,711 (GRCm38)	N662K	possibly damaging	Het

Other mutations in Fras1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Fras1	APN	5	96,739,358 (GRCm38)	missense	possibly damaging	0.55
IGL00507:Fras1	APN	5	96,778,189 (GRCm38)	missense	probably damaging	1.00
IGL00672:Fras1	APN	5	96,759,450 (GRCm38)	splice site	probably benign
IGL00772:Fras1	APN	5	96,636,112 (GRCm38)	missense	probably benign	0.42
IGL00844:Fras1	APN	5	96,534,853 (GRCm38)	splice site	probably benign
IGL00913:Fras1	APN	5	96,695,076 (GRCm38)	missense	probably damaging	0.99
IGL00959:Fras1	APN	5	96,781,281 (GRCm38)	missense	probably damaging	0.96
IGL00966:Fras1	APN	5	96,555,221 (GRCm38)	missense	probably benign	0.00
IGL01296:Fras1	APN	5	96,673,698 (GRCm38)	missense	probably null	0.58
IGL01307:Fras1	APN	5	96,781,692 (GRCm38)	missense	probably benign
IGL01481:Fras1	APN	5	96,657,241 (GRCm38)	missense	probably damaging	1.00
IGL01525:Fras1	APN	5	96,739,336 (GRCm38)	missense	probably damaging	0.99
IGL01599:Fras1	APN	5	96,709,891 (GRCm38)	missense	possibly damaging	0.94
IGL01646:Fras1	APN	5	96,758,148 (GRCm38)	missense	probably benign	0.29
IGL01795:Fras1	APN	5	96,778,045 (GRCm38)	missense	probably damaging	1.00
IGL01867:Fras1	APN	5	96,588,131 (GRCm38)	missense	probably benign
IGL01869:Fras1	APN	5	96,708,783 (GRCm38)	splice site	probably benign
IGL01923:Fras1	APN	5	96,735,280 (GRCm38)	missense	probably damaging	1.00
IGL01982:Fras1	APN	5	96,739,248 (GRCm38)	missense	possibly damaging	0.46
IGL02109:Fras1	APN	5	96,700,523 (GRCm38)	missense	probably benign
IGL02132:Fras1	APN	5	96,781,637 (GRCm38)	nonsense	probably null
IGL02171:Fras1	APN	5	96,735,181 (GRCm38)	missense	probably benign	0.15
IGL02213:Fras1	APN	5	96,645,871 (GRCm38)	nonsense	probably null
IGL02277:Fras1	APN	5	96,588,118 (GRCm38)	missense	probably benign	0.00
IGL02507:Fras1	APN	5	96,657,408 (GRCm38)	missense	possibly damaging	0.95
IGL02589:Fras1	APN	5	96,769,513 (GRCm38)	missense	probably damaging	1.00
IGL02671:Fras1	APN	5	96,728,616 (GRCm38)	missense	possibly damaging	0.91
IGL02677:Fras1	APN	5	96,545,024 (GRCm38)	missense	probably damaging	1.00
IGL02691:Fras1	APN	5	96,744,705 (GRCm38)	missense	possibly damaging	0.68
IGL02741:Fras1	APN	5	96,691,371 (GRCm38)	missense	probably benign	0.35
IGL02836:Fras1	APN	5	96,534,866 (GRCm38)	missense	possibly damaging	0.67
IGL02850:Fras1	APN	5	96,778,175 (GRCm38)	missense	probably damaging	1.00
IGL02998:Fras1	APN	5	96,702,181 (GRCm38)	missense	possibly damaging	0.82
IGL03040:Fras1	APN	5	96,710,101 (GRCm38)	missense	probably benign
IGL03078:Fras1	APN	5	96,636,135 (GRCm38)	missense	probably damaging	1.00
IGL03096:Fras1	APN	5	96,764,901 (GRCm38)	missense	probably damaging	1.00
IGL03102:Fras1	APN	5	96,726,535 (GRCm38)	missense	probably benign	0.11
IGL03183:Fras1	APN	5	96,733,781 (GRCm38)	splice site	probably benign
IGL03189:Fras1	APN	5	96,743,071 (GRCm38)	missense	probably benign	0.00
IGL03193:Fras1	APN	5	96,778,106 (GRCm38)	missense	probably damaging	0.99
IGL03292:Fras1	APN	5	96,707,491 (GRCm38)	missense	probably damaging	1.00
IGL03328:Fras1	APN	5	96,781,760 (GRCm38)	missense	probably damaging	0.96
IGL03335:Fras1	APN	5	96,733,944 (GRCm38)	splice site	probably benign
IGL03394:Fras1	APN	5	96,667,477 (GRCm38)	missense	probably damaging	0.98
IGL03404:Fras1	APN	5	96,728,581 (GRCm38)	missense	probably damaging	0.99
baby_ruth	UTSW	5	96,708,758 (GRCm38)	missense	probably benign	0.01
BB002:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
BB012:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
G1patch:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
I0000:Fras1	UTSW	5	96,740,829 (GRCm38)	missense	probably damaging	0.99
PIT4581001:Fras1	UTSW	5	96,555,301 (GRCm38)	missense	probably benign	0.01
R0028:Fras1	UTSW	5	96,677,316 (GRCm38)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,776,622 (GRCm38)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,776,622 (GRCm38)	missense	probably benign	0.07
R0099:Fras1	UTSW	5	96,614,917 (GRCm38)	critical splice donor site	probably null
R0109:Fras1	UTSW	5	96,710,077 (GRCm38)	missense	probably benign	0.01
R0158:Fras1	UTSW	5	96,776,634 (GRCm38)	missense	possibly damaging	0.83
R0268:Fras1	UTSW	5	96,737,009 (GRCm38)	missense	probably damaging	0.99
R0305:Fras1	UTSW	5	96,596,888 (GRCm38)	missense	probably benign
R0352:Fras1	UTSW	5	96,726,540 (GRCm38)	missense	probably damaging	0.97
R0359:Fras1	UTSW	5	96,762,590 (GRCm38)	missense	probably damaging	0.98
R0371:Fras1	UTSW	5	96,555,331 (GRCm38)	missense	possibly damaging	0.90
R0379:Fras1	UTSW	5	96,755,509 (GRCm38)	nonsense	probably null
R0395:Fras1	UTSW	5	96,769,653 (GRCm38)	missense	possibly damaging	0.50
R0417:Fras1	UTSW	5	96,691,372 (GRCm38)	missense	probably benign	0.18
R0454:Fras1	UTSW	5	96,762,665 (GRCm38)	missense	probably damaging	0.96
R0456:Fras1	UTSW	5	96,714,343 (GRCm38)	splice site	probably null
R0456:Fras1	UTSW	5	96,554,788 (GRCm38)	missense	probably damaging	1.00
R0464:Fras1	UTSW	5	96,636,803 (GRCm38)	missense	probably damaging	0.98
R0613:Fras1	UTSW	5	96,700,488 (GRCm38)	splice site	probably benign
R0652:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
R0675:Fras1	UTSW	5	96,667,387 (GRCm38)	splice site	probably benign
R0765:Fras1	UTSW	5	96,552,796 (GRCm38)	missense	probably benign	0.00
R0783:Fras1	UTSW	5	96,768,430 (GRCm38)	missense	probably damaging	1.00
R0811:Fras1	UTSW	5	96,752,998 (GRCm38)	missense	probably benign	0.35
R0812:Fras1	UTSW	5	96,752,998 (GRCm38)	missense	probably benign	0.35
R0943:Fras1	UTSW	5	96,726,543 (GRCm38)	missense	probably benign	0.00
R1037:Fras1	UTSW	5	96,714,463 (GRCm38)	missense	probably damaging	0.97
R1104:Fras1	UTSW	5	96,708,671 (GRCm38)	missense	probably benign	0.00
R1108:Fras1	UTSW	5	96,642,629 (GRCm38)	missense	probably damaging	0.99
R1332:Fras1	UTSW	5	96,707,308 (GRCm38)	missense	probably benign	0.00
R1336:Fras1	UTSW	5	96,707,308 (GRCm38)	missense	probably benign	0.00
R1458:Fras1	UTSW	5	96,600,733 (GRCm38)	missense	probably benign	0.00
R1495:Fras1	UTSW	5	96,528,586 (GRCm38)	missense	possibly damaging	0.49
R1499:Fras1	UTSW	5	96,743,187 (GRCm38)	missense	probably benign	0.31
R1528:Fras1	UTSW	5	96,636,819 (GRCm38)	missense	probably damaging	0.99
R1532:Fras1	UTSW	5	96,713,996 (GRCm38)	missense	probably damaging	1.00
R1556:Fras1	UTSW	5	96,743,062 (GRCm38)	missense	possibly damaging	0.88
R1625:Fras1	UTSW	5	96,709,978 (GRCm38)	missense	possibly damaging	0.94
R1625:Fras1	UTSW	5	96,713,990 (GRCm38)	missense	probably damaging	1.00
R1645:Fras1	UTSW	5	96,700,586 (GRCm38)	missense	possibly damaging	0.90
R1647:Fras1	UTSW	5	96,726,613 (GRCm38)	critical splice donor site	probably null
R1648:Fras1	UTSW	5	96,726,613 (GRCm38)	critical splice donor site	probably null
R1661:Fras1	UTSW	5	96,598,909 (GRCm38)	missense	probably damaging	1.00
R1665:Fras1	UTSW	5	96,598,909 (GRCm38)	missense	probably damaging	1.00
R1682:Fras1	UTSW	5	96,645,873 (GRCm38)	missense	probably benign	0.00
R1701:Fras1	UTSW	5	96,600,784 (GRCm38)	missense	probably benign	0.00
R1716:Fras1	UTSW	5	96,552,725 (GRCm38)	missense	probably benign	0.10
R1718:Fras1	UTSW	5	96,554,889 (GRCm38)	splice site	probably null
R1800:Fras1	UTSW	5	96,709,882 (GRCm38)	missense	probably benign
R1806:Fras1	UTSW	5	96,764,976 (GRCm38)	missense	possibly damaging	0.88
R1806:Fras1	UTSW	5	96,713,970 (GRCm38)	splice site	probably benign
R1822:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1823:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1824:Fras1	UTSW	5	96,770,688 (GRCm38)	missense	probably damaging	1.00
R1847:Fras1	UTSW	5	96,749,423 (GRCm38)	splice site	probably null
R1929:Fras1	UTSW	5	96,667,437 (GRCm38)	missense	probably benign	0.24
R1951:Fras1	UTSW	5	96,712,383 (GRCm38)	missense	probably benign	0.38
R2093:Fras1	UTSW	5	96,781,203 (GRCm38)	missense	probably damaging	1.00
R2283:Fras1	UTSW	5	96,654,305 (GRCm38)	missense	probably benign	0.10
R2884:Fras1	UTSW	5	96,700,268 (GRCm38)	missense	probably benign	0.07
R2913:Fras1	UTSW	5	96,733,915 (GRCm38)	missense	probably benign
R2914:Fras1	UTSW	5	96,733,915 (GRCm38)	missense	probably benign
R3054:Fras1	UTSW	5	96,764,943 (GRCm38)	missense	probably damaging	0.99
R3117:Fras1	UTSW	5	96,771,712 (GRCm38)	missense	probably damaging	1.00
R3118:Fras1	UTSW	5	96,771,712 (GRCm38)	missense	probably damaging	1.00
R3691:Fras1	UTSW	5	96,781,512 (GRCm38)	missense	probably benign	0.02
R3714:Fras1	UTSW	5	96,645,970 (GRCm38)	critical splice donor site	probably null
R3715:Fras1	UTSW	5	96,645,970 (GRCm38)	critical splice donor site	probably null
R3801:Fras1	UTSW	5	96,733,932 (GRCm38)	missense	probably benign	0.26
R3961:Fras1	UTSW	5	96,677,385 (GRCm38)	critical splice donor site	probably null
R4065:Fras1	UTSW	5	96,770,683 (GRCm38)	missense	possibly damaging	0.64
R4066:Fras1	UTSW	5	96,770,683 (GRCm38)	missense	possibly damaging	0.64
R4076:Fras1	UTSW	5	96,743,158 (GRCm38)	missense	probably damaging	1.00
R4124:Fras1	UTSW	5	96,770,653 (GRCm38)	missense	probably benign	0.05
R4127:Fras1	UTSW	5	96,770,653 (GRCm38)	missense	probably benign	0.05
R4153:Fras1	UTSW	5	96,776,735 (GRCm38)	missense	probably benign	0.17
R4233:Fras1	UTSW	5	96,714,376 (GRCm38)	missense	possibly damaging	0.91
R4273:Fras1	UTSW	5	96,614,904 (GRCm38)	missense	probably benign	0.00
R4355:Fras1	UTSW	5	96,700,242 (GRCm38)	missense	probably benign
R4401:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4402:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4403:Fras1	UTSW	5	96,642,620 (GRCm38)	missense	probably damaging	0.97
R4505:Fras1	UTSW	5	96,781,348 (GRCm38)	missense	probably damaging	1.00
R4548:Fras1	UTSW	5	96,709,895 (GRCm38)	missense	probably benign	0.00
R4559:Fras1	UTSW	5	96,781,289 (GRCm38)	missense	probably damaging	1.00
R4629:Fras1	UTSW	5	96,776,734 (GRCm38)	missense	probably benign	0.00
R4637:Fras1	UTSW	5	96,778,088 (GRCm38)	missense	probably damaging	1.00
R4678:Fras1	UTSW	5	96,700,568 (GRCm38)	missense	probably benign	0.13
R4707:Fras1	UTSW	5	96,735,238 (GRCm38)	missense	probably damaging	0.96
R4735:Fras1	UTSW	5	96,588,163 (GRCm38)	missense	probably benign	0.00
R4756:Fras1	UTSW	5	96,781,659 (GRCm38)	missense	probably benign	0.00
R4762:Fras1	UTSW	5	96,731,618 (GRCm38)	missense	probably benign
R4820:Fras1	UTSW	5	96,728,653 (GRCm38)	missense	probably benign	0.00
R4847:Fras1	UTSW	5	96,544,992 (GRCm38)	missense	possibly damaging	0.94
R4857:Fras1	UTSW	5	96,778,159 (GRCm38)	missense	probably benign	0.00
R4909:Fras1	UTSW	5	96,708,758 (GRCm38)	missense	probably benign	0.01
R4931:Fras1	UTSW	5	96,636,840 (GRCm38)	missense	probably benign	0.02
R4938:Fras1	UTSW	5	96,776,724 (GRCm38)	missense	probably damaging	0.99
R4952:Fras1	UTSW	5	96,647,498 (GRCm38)	missense	probably benign	0.01
R4965:Fras1	UTSW	5	96,726,580 (GRCm38)	missense	possibly damaging	0.95
R4989:Fras1	UTSW	5	96,650,682 (GRCm38)	missense	possibly damaging	0.75
R5151:Fras1	UTSW	5	96,645,110 (GRCm38)	missense	probably damaging	1.00
R5168:Fras1	UTSW	5	96,708,757 (GRCm38)	missense	probably benign	0.00
R5214:Fras1	UTSW	5	96,769,593 (GRCm38)	missense	probably damaging	1.00
R5220:Fras1	UTSW	5	96,768,363 (GRCm38)	missense	probably damaging	1.00
R5235:Fras1	UTSW	5	96,600,750 (GRCm38)	missense	probably benign	0.02
R5242:Fras1	UTSW	5	96,657,250 (GRCm38)	missense	probably benign	0.11
R5253:Fras1	UTSW	5	96,741,025 (GRCm38)	missense	probably damaging	0.99
R5260:Fras1	UTSW	5	96,735,187 (GRCm38)	missense	possibly damaging	0.79
R5301:Fras1	UTSW	5	96,657,266 (GRCm38)	missense	possibly damaging	0.88
R5411:Fras1	UTSW	5	96,645,160 (GRCm38)	missense	probably benign	0.00
R5467:Fras1	UTSW	5	96,780,053 (GRCm38)	missense	probably benign	0.04
R5543:Fras1	UTSW	5	96,528,535 (GRCm38)	missense	probably benign	0.01
R5555:Fras1	UTSW	5	96,677,377 (GRCm38)	missense	probably benign	0.34
R5602:Fras1	UTSW	5	96,737,021 (GRCm38)	missense	probably damaging	1.00
R5664:Fras1	UTSW	5	96,728,535 (GRCm38)	missense	possibly damaging	0.91
R5695:Fras1	UTSW	5	96,781,344 (GRCm38)	missense	probably damaging	1.00
R5717:Fras1	UTSW	5	96,781,737 (GRCm38)	missense	possibly damaging	0.93
R5742:Fras1	UTSW	5	96,768,381 (GRCm38)	missense	possibly damaging	0.50
R5759:Fras1	UTSW	5	96,709,916 (GRCm38)	missense	probably benign	0.02
R5766:Fras1	UTSW	5	96,731,689 (GRCm38)	missense	possibly damaging	0.91
R5890:Fras1	UTSW	5	96,645,948 (GRCm38)	missense	probably benign
R6052:Fras1	UTSW	5	96,764,866 (GRCm38)	missense	probably damaging	1.00
R6058:Fras1	UTSW	5	96,709,985 (GRCm38)	missense	probably benign
R6256:Fras1	UTSW	5	96,733,843 (GRCm38)	missense	possibly damaging	0.84
R6306:Fras1	UTSW	5	96,764,946 (GRCm38)	missense	probably damaging	1.00
R6494:Fras1	UTSW	5	96,759,564 (GRCm38)	missense	possibly damaging	0.59
R6638:Fras1	UTSW	5	96,758,094 (GRCm38)	missense	possibly damaging	0.94
R6647:Fras1	UTSW	5	96,735,202 (GRCm38)	missense	probably damaging	1.00
R6725:Fras1	UTSW	5	96,781,340 (GRCm38)	missense	possibly damaging	0.91
R6769:Fras1	UTSW	5	96,598,941 (GRCm38)	missense	possibly damaging	0.60
R6771:Fras1	UTSW	5	96,598,941 (GRCm38)	missense	possibly damaging	0.60
R6837:Fras1	UTSW	5	96,726,973 (GRCm38)	missense	probably damaging	0.99
R6841:Fras1	UTSW	5	96,728,551 (GRCm38)	missense	probably damaging	0.99
R6863:Fras1	UTSW	5	96,543,306 (GRCm38)	missense	probably benign	0.19
R6868:Fras1	UTSW	5	96,682,378 (GRCm38)	missense	probably benign	0.38
R6936:Fras1	UTSW	5	96,768,352 (GRCm38)	missense	possibly damaging	0.92
R6997:Fras1	UTSW	5	96,614,873 (GRCm38)	nonsense	probably null
R7023:Fras1	UTSW	5	96,710,084 (GRCm38)	missense	probably benign	0.00
R7091:Fras1	UTSW	5	96,708,676 (GRCm38)	missense	probably benign
R7102:Fras1	UTSW	5	96,571,041 (GRCm38)	missense	probably benign
R7120:Fras1	UTSW	5	96,752,960 (GRCm38)	nonsense	probably null
R7124:Fras1	UTSW	5	96,714,401 (GRCm38)	missense	probably damaging	1.00
R7129:Fras1	UTSW	5	96,781,284 (GRCm38)	missense	probably benign	0.00
R7173:Fras1	UTSW	5	96,778,078 (GRCm38)	missense	probably damaging	1.00
R7174:Fras1	UTSW	5	96,755,577 (GRCm38)	critical splice donor site	probably null
R7185:Fras1	UTSW	5	96,636,776 (GRCm38)	missense	probably damaging	1.00
R7191:Fras1	UTSW	5	96,614,912 (GRCm38)	missense	probably benign	0.05
R7216:Fras1	UTSW	5	96,739,314 (GRCm38)	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96,636,809 (GRCm38)	missense	probably benign	0.00
R7222:Fras1	UTSW	5	96,636,186 (GRCm38)	missense	probably damaging	1.00
R7320:Fras1	UTSW	5	96,709,886 (GRCm38)	missense	probably benign	0.03
R7335:Fras1	UTSW	5	96,736,970 (GRCm38)	missense	possibly damaging	0.82
R7378:Fras1	UTSW	5	96,596,785 (GRCm38)	missense	probably damaging	0.98
R7394:Fras1	UTSW	5	96,712,450 (GRCm38)	nonsense	probably null
R7412:Fras1	UTSW	5	96,614,889 (GRCm38)	missense	probably benign	0.06
R7422:Fras1	UTSW	5	96,673,599 (GRCm38)	missense	probably benign	0.21
R7552:Fras1	UTSW	5	96,768,438 (GRCm38)	missense	probably damaging	1.00
R7559:Fras1	UTSW	5	96,740,854 (GRCm38)	missense	possibly damaging	0.82
R7575:Fras1	UTSW	5	96,543,314 (GRCm38)	missense	probably benign	0.02
R7578:Fras1	UTSW	5	96,684,437 (GRCm38)	missense	probably damaging	1.00
R7600:Fras1	UTSW	5	96,684,436 (GRCm38)	missense	probably damaging	1.00
R7669:Fras1	UTSW	5	96,692,624 (GRCm38)	missense	probably benign	0.01
R7710:Fras1	UTSW	5	96,645,103 (GRCm38)	nonsense	probably null
R7722:Fras1	UTSW	5	96,769,554 (GRCm38)	missense	probably damaging	1.00
R7726:Fras1	UTSW	5	96,712,451 (GRCm38)	missense	probably benign	0.41
R7745:Fras1	UTSW	5	96,726,895 (GRCm38)	missense	probably benign	0.11
R7777:Fras1	UTSW	5	96,752,904 (GRCm38)	missense	probably damaging	1.00
R7923:Fras1	UTSW	5	96,739,318 (GRCm38)	missense	probably damaging	1.00
R7925:Fras1	UTSW	5	96,781,584 (GRCm38)	missense	probably damaging	0.96
R8000:Fras1	UTSW	5	96,762,677 (GRCm38)	missense	probably damaging	0.96
R8056:Fras1	UTSW	5	96,744,774 (GRCm38)	missense	probably damaging	1.00
R8058:Fras1	UTSW	5	96,694,919 (GRCm38)	missense	probably benign
R8117:Fras1	UTSW	5	96,707,386 (GRCm38)	missense	probably damaging	1.00
R8157:Fras1	UTSW	5	96,554,855 (GRCm38)	missense	probably benign	0.00
R8312:Fras1	UTSW	5	96,588,191 (GRCm38)	missense	probably benign
R8315:Fras1	UTSW	5	96,743,182 (GRCm38)	missense	probably damaging	0.97
R8412:Fras1	UTSW	5	96,596,852 (GRCm38)	missense	probably benign	0.00
R8546:Fras1	UTSW	5	96,709,966 (GRCm38)	missense	probably benign	0.00
R8705:Fras1	UTSW	5	96,691,401 (GRCm38)	missense	probably benign	0.01
R8790:Fras1	UTSW	5	96,755,377 (GRCm38)	missense	probably damaging	1.00
R8848:Fras1	UTSW	5	96,781,348 (GRCm38)	missense	probably damaging	1.00
R8855:Fras1	UTSW	5	96,769,606 (GRCm38)	missense
R8871:Fras1	UTSW	5	96,707,398 (GRCm38)	missense	probably benign	0.34
R8894:Fras1	UTSW	5	96,759,543 (GRCm38)	missense	probably damaging	1.00
R8904:Fras1	UTSW	5	96,781,279 (GRCm38)	missense	probably benign	0.16
R8910:Fras1	UTSW	5	96,567,996 (GRCm38)	missense	probably benign	0.01
R8916:Fras1	UTSW	5	96,752,915 (GRCm38)	missense	probably damaging	1.00
R8929:Fras1	UTSW	5	96,769,507 (GRCm38)	missense	probably damaging	0.98
R8995:Fras1	UTSW	5	96,712,556 (GRCm38)	missense	possibly damaging	0.83
R9016:Fras1	UTSW	5	96,636,064 (GRCm38)	missense	probably damaging	0.96
R9021:Fras1	UTSW	5	96,740,750 (GRCm38)	missense	probably damaging	1.00
R9038:Fras1	UTSW	5	96,726,883 (GRCm38)	missense	probably benign	0.25
R9204:Fras1	UTSW	5	96,735,163 (GRCm38)	missense	probably damaging	1.00
R9222:Fras1	UTSW	5	96,657,228 (GRCm38)	missense	probably benign	0.15
R9231:Fras1	UTSW	5	96,545,045 (GRCm38)	missense	probably damaging	1.00
R9238:Fras1	UTSW	5	96,684,361 (GRCm38)	missense	possibly damaging	0.90
R9257:Fras1	UTSW	5	96,762,500 (GRCm38)	missense	probably damaging	1.00
R9302:Fras1	UTSW	5	96,534,892 (GRCm38)	missense	probably damaging	1.00
R9329:Fras1	UTSW	5	96,736,954 (GRCm38)	missense	probably damaging	1.00
R9361:Fras1	UTSW	5	96,776,698 (GRCm38)	missense	probably damaging	1.00
R9430:Fras1	UTSW	5	96,781,392 (GRCm38)	missense	probably benign	0.00
R9431:Fras1	UTSW	5	96,753,014 (GRCm38)	missense	possibly damaging	0.71
R9474:Fras1	UTSW	5	96,739,265 (GRCm38)	missense	probably benign	0.00
R9475:Fras1	UTSW	5	96,780,070 (GRCm38)	missense	probably damaging	0.96
R9497:Fras1	UTSW	5	96,737,036 (GRCm38)	missense	probably damaging	1.00
R9597:Fras1	UTSW	5	96,740,692 (GRCm38)	missense	probably damaging	1.00
R9650:Fras1	UTSW	5	96,762,528 (GRCm38)	missense	probably damaging	1.00
R9694:Fras1	UTSW	5	96,781,686 (GRCm38)	missense	probably benign	0.03
R9779:Fras1	UTSW	5	96,569,494 (GRCm38)	missense	probably damaging	0.99
Z1088:Fras1	UTSW	5	96,743,211 (GRCm38)	missense	probably damaging	0.97
Z1088:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1176:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1177:Fras1	UTSW	5	96,700,251 (GRCm38)	missense	possibly damaging	0.71
Z1177:Fras1	UTSW	5	96,691,457 (GRCm38)	missense	probably damaging	1.00
Z1177:Fras1	UTSW	5	96,758,142 (GRCm38)	missense	probably benign
Z1177:Fras1	UTSW	5	96,740,983 (GRCm38)	missense	possibly damaging	0.95
Z1177:Fras1	UTSW	5	96,740,811 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- TCCTCTCTGGATGGCTGAATGG -3'
(R):5'- CCAAACAGGGTATGGGGCC -3'

Sequencing Primer
(F):5'- AGCTGTGGTAATTGAACCCC -3'
(R):5'- TATGGGGCCAGGCTGCAG -3'

Posted On

2016-07-06