Mutagenetix > Incidental Mutation

Incidental Mutation 'R5182:Vmn2r114'

397639

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r114

Ensembl Gene

ENSMUSG00000091945

Gene Name

vomeronasal 2, receptor 114

Synonyms

EG666002

MMRRC Submission

042853-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.142) question?

Stock #

R5182 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23509908-23531287 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 23510632 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 616 (V616A)

Ref Sequence

ENSEMBL: ENSMUSP00000127505 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168033]

AlphaFold

E9Q281

Predicted Effect

probably damaging
Transcript: ENSMUST00000168033
AA Change: V616A

PolyPhen 2 Score 0.963 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000127505
Gene: ENSMUSG00000091945
AA Change: V616A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	470	1.5e-24	PFAM
Pfam:NCD3G	511	564	1.5e-18	PFAM
Pfam:7tm_3	597	832	1.4e-55	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

100% (66/66)

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059E24Rik	A	G	19: 21,608,129 (GRCm39)	M1T	probably null	Het
Abcg8	T	C	17: 85,000,172 (GRCm39)	L243P	probably damaging	Het
Ankrd27	A	G	7: 35,327,912 (GRCm39)	T811A	probably damaging	Het
Auts2	T	C	5: 131,503,919 (GRCm39)	N188S	probably null	Het
Bak1	G	A	17: 27,241,722 (GRCm39)	P65L	possibly damaging	Het
Cep350	A	C	1: 155,733,854 (GRCm39)	L3013R	probably damaging	Het
Clca3b	A	G	3: 144,533,776 (GRCm39)	I533T	probably damaging	Het
Col6a5	C	A	9: 105,734,531 (GRCm39)	E2637*	probably null	Het
Coq5	G	A	5: 115,417,815 (GRCm39)	R15H	probably benign	Het
Dnah5	T	G	15: 28,311,424 (GRCm39)	I1801S	probably damaging	Het
Dnhd1	G	A	7: 105,352,416 (GRCm39)	R2523Q	probably damaging	Het
Dpf3	T	C	12: 83,417,370 (GRCm39)	E34G	probably damaging	Het
Dst	C	A	1: 34,218,167 (GRCm39)	Q1536K	probably benign	Het
Eeig2	T	C	3: 108,892,667 (GRCm39)	K173E	possibly damaging	Het
Eng	T	C	2: 32,562,971 (GRCm39)		probably null	Het
Fignl1	A	T	11: 11,751,717 (GRCm39)	I446N	probably damaging	Het
Fras1	C	A	5: 96,784,032 (GRCm39)	C845*	probably null	Het
Gfm2	T	C	13: 97,299,401 (GRCm39)	V347A	probably damaging	Het
Gpld1	A	T	13: 25,168,053 (GRCm39)		probably null	Het
Grn	C	A	11: 102,321,380 (GRCm39)		probably benign	Het
Gsdmc4	C	A	15: 63,765,653 (GRCm39)	V299F	probably damaging	Het
Hrnr	A	T	3: 93,239,450 (GRCm39)	R3229S	unknown	Het
Icam5	A	G	9: 20,946,106 (GRCm39)	T313A	probably benign	Het
Ift172	T	A	5: 31,424,958 (GRCm39)	D668V	possibly damaging	Het
Kdm5a	T	A	6: 120,365,066 (GRCm39)	C322*	probably null	Het
Klk6	A	G	7: 43,478,084 (GRCm39)	K152R	probably benign	Het
Man1a2	A	C	3: 100,554,333 (GRCm39)	I250S	probably damaging	Het
Nkpd1	A	G	7: 19,257,181 (GRCm39)	Y170C	probably damaging	Het
Nmd3	T	A	3: 69,629,801 (GRCm39)		probably null	Het
Or2y3	A	G	17: 38,393,005 (GRCm39)	M288T	probably benign	Het
Or51f1	A	T	7: 102,506,176 (GRCm39)	D104E	probably benign	Het
Pax4	C	T	6: 28,444,368 (GRCm39)	E229K	probably benign	Het
Pdc	T	C	1: 150,209,105 (GRCm39)	I196T	possibly damaging	Het
Pld1	T	C	3: 28,099,230 (GRCm39)	I299T	probably damaging	Het
Pnma1	T	C	12: 84,193,819 (GRCm39)	I295V	probably benign	Het
Prkg2	T	A	5: 99,172,568 (GRCm39)	Y49F	probably benign	Het
Psmd12	T	C	11: 107,370,485 (GRCm39)	L28P	probably damaging	Het
Ptprg	A	G	14: 12,154,174 (GRCm38)	T632A	probably benign	Het
Rab43	A	G	6: 87,771,637 (GRCm39)	*118R	probably null	Het
Rabep1	C	T	11: 70,795,454 (GRCm39)	R227*	probably null	Het
Rad51c	T	G	11: 87,288,545 (GRCm39)	I213L	possibly damaging	Het
Ranbp17	A	T	11: 33,169,287 (GRCm39)		probably benign	Het
Ryr3	T	C	2: 112,585,495 (GRCm39)	E2735G	probably damaging	Het
Sdc3	A	G	4: 130,548,995 (GRCm39)		probably benign	Het
Sirpa	G	A	2: 129,457,652 (GRCm39)	R242H	possibly damaging	Het
Slc12a4	G	A	8: 106,671,238 (GRCm39)	T983M	probably damaging	Het
Snx7	A	G	3: 117,626,506 (GRCm39)	Y252H	probably damaging	Het
St18	C	T	1: 6,887,877 (GRCm39)	T482I	probably benign	Het
St7	A	G	6: 17,846,236 (GRCm39)	Y163C	probably damaging	Het
Tas2r140	A	T	6: 40,468,866 (GRCm39)	Q232L	probably benign	Het
Tgm6	A	T	2: 129,983,222 (GRCm39)	K270N	probably damaging	Het
Tiam2	G	A	17: 3,488,996 (GRCm39)	G768D	probably damaging	Het
Ttn	C	T	2: 76,700,773 (GRCm39)		probably benign	Het
Ube4b	A	C	4: 149,465,699 (GRCm39)	S250R	probably null	Het
Ubxn2a	G	A	12: 4,930,634 (GRCm39)	A242V	probably damaging	Het
Usp10	G	A	8: 120,683,420 (GRCm39)	V764I	possibly damaging	Het
Vmn1r222	A	G	13: 23,416,667 (GRCm39)	L182P	probably damaging	Het
Vps13a	A	G	19: 16,672,863 (GRCm39)	V1303A	possibly damaging	Het
Wnk2	G	T	13: 49,214,637 (GRCm39)	T1315K	possibly damaging	Het
Zfp936	G	A	7: 42,839,331 (GRCm39)	C266Y	probably damaging	Het
Zscan20	G	T	4: 128,480,504 (GRCm39)	N662K	possibly damaging	Het

Other mutations in Vmn2r114

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Vmn2r114	APN	17	23,510,639 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,510,212 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,509,957 (GRCm39)	missense	probably benign	0.23
IGL00990:Vmn2r114	APN	17	23,509,939 (GRCm39)	missense	probably benign
IGL01838:Vmn2r114	APN	17	23,515,956 (GRCm39)	missense	probably benign	0.44
IGL01990:Vmn2r114	APN	17	23,529,355 (GRCm39)	missense	probably benign	0.22
IGL01994:Vmn2r114	APN	17	23,529,451 (GRCm39)	missense	probably damaging	1.00
IGL02153:Vmn2r114	APN	17	23,510,782 (GRCm39)	missense	probably benign	0.01
IGL02453:Vmn2r114	APN	17	23,530,108 (GRCm39)	missense	probably benign	0.00
IGL02621:Vmn2r114	APN	17	23,529,494 (GRCm39)	missense	probably damaging	0.98
IGL02938:Vmn2r114	APN	17	23,510,263 (GRCm39)	missense	probably benign	0.10
IGL03130:Vmn2r114	APN	17	23,515,970 (GRCm39)	splice site	probably benign
IGL03325:Vmn2r114	APN	17	23,510,652 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r114	UTSW	17	23,510,619 (GRCm39)	missense	probably damaging	1.00
R0109:Vmn2r114	UTSW	17	23,529,549 (GRCm39)	nonsense	probably null
R0164:Vmn2r114	UTSW	17	23,528,800 (GRCm39)	critical splice donor site	probably null
R0310:Vmn2r114	UTSW	17	23,509,917 (GRCm39)	missense	probably benign	0.23
R0583:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R0677:Vmn2r114	UTSW	17	23,529,568 (GRCm39)	missense	probably damaging	1.00
R1127:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1157:Vmn2r114	UTSW	17	23,529,314 (GRCm39)	missense	possibly damaging	0.60
R1323:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1347:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1435:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1437:Vmn2r114	UTSW	17	23,510,185 (GRCm39)	missense	probably damaging	1.00
R1585:Vmn2r114	UTSW	17	23,510,675 (GRCm39)	missense	probably damaging	0.98
R1641:Vmn2r114	UTSW	17	23,515,962 (GRCm39)	missense	probably benign	0.00
R1748:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably benign	0.17
R1954:Vmn2r114	UTSW	17	23,530,086 (GRCm39)	missense	probably benign	0.32
R2081:Vmn2r114	UTSW	17	23,510,083 (GRCm39)	missense	possibly damaging	0.91
R2103:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2113:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2134:Vmn2r114	UTSW	17	23,510,737 (GRCm39)	missense	probably damaging	1.00
R2149:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2424:Vmn2r114	UTSW	17	23,515,842 (GRCm39)	missense	possibly damaging	0.90
R2847:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2848:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2893:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3017:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3018:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3019:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3020:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3021:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4628:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4668:Vmn2r114	UTSW	17	23,529,447 (GRCm39)	missense	possibly damaging	0.83
R4840:Vmn2r114	UTSW	17	23,510,353 (GRCm39)	missense	probably damaging	0.97
R4841:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4842:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4856:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4886:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4992:Vmn2r114	UTSW	17	23,510,765 (GRCm39)	missense	probably benign	0.03
R5223:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5405:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5449:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5615:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5834:Vmn2r114	UTSW	17	23,529,599 (GRCm39)	missense	possibly damaging	0.90
R6150:Vmn2r114	UTSW	17	23,510,269 (GRCm39)	missense	probably benign	0.03
R6277:Vmn2r114	UTSW	17	23,509,954 (GRCm39)	missense	possibly damaging	0.93
R6403:Vmn2r114	UTSW	17	23,528,939 (GRCm39)	missense	probably damaging	0.99
R6589:Vmn2r114	UTSW	17	23,510,642 (GRCm39)	missense	probably damaging	1.00
R6613:Vmn2r114	UTSW	17	23,529,220 (GRCm39)	missense	possibly damaging	0.82
R6747:Vmn2r114	UTSW	17	23,528,850 (GRCm39)	missense	probably benign	0.00
R6837:Vmn2r114	UTSW	17	23,529,176 (GRCm39)	missense	probably benign	0.10
R6911:Vmn2r114	UTSW	17	23,510,104 (GRCm39)	missense	probably damaging	0.98
R6950:Vmn2r114	UTSW	17	23,529,137 (GRCm39)	missense	probably benign	0.03
R7276:Vmn2r114	UTSW	17	23,509,934 (GRCm39)	missense	probably damaging	0.97
R7482:Vmn2r114	UTSW	17	23,510,468 (GRCm39)	missense	probably damaging	1.00
R7514:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably null	0.96
R7523:Vmn2r114	UTSW	17	23,529,611 (GRCm39)	missense	probably benign	0.01
R7563:Vmn2r114	UTSW	17	23,510,000 (GRCm39)	missense	probably benign	0.01
R7585:Vmn2r114	UTSW	17	23,510,239 (GRCm39)	missense	probably damaging	1.00
R7593:Vmn2r114	UTSW	17	23,510,817 (GRCm39)	nonsense	probably null
R7611:Vmn2r114	UTSW	17	23,515,944 (GRCm39)	missense	probably damaging	0.97
R7641:Vmn2r114	UTSW	17	23,527,177 (GRCm39)	missense	possibly damaging	0.53
R7651:Vmn2r114	UTSW	17	23,509,986 (GRCm39)	nonsense	probably null
R7970:Vmn2r114	UTSW	17	23,530,186 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r114	UTSW	17	23,529,142 (GRCm39)	missense	probably benign	0.36
R8802:Vmn2r114	UTSW	17	23,528,836 (GRCm39)	missense	possibly damaging	0.65
R8847:Vmn2r114	UTSW	17	23,528,986 (GRCm39)	missense	probably damaging	1.00
R8991:Vmn2r114	UTSW	17	23,529,286 (GRCm39)	missense	probably damaging	1.00
R9138:Vmn2r114	UTSW	17	23,510,578 (GRCm39)	missense	probably damaging	1.00
R9173:Vmn2r114	UTSW	17	23,510,527 (GRCm39)	missense	probably damaging	0.99
R9175:Vmn2r114	UTSW	17	23,527,212 (GRCm39)	missense	probably damaging	1.00
R9657:Vmn2r114	UTSW	17	23,510,690 (GRCm39)	missense	probably damaging	1.00
R9670:Vmn2r114	UTSW	17	23,531,098 (GRCm39)	missense
X0065:Vmn2r114	UTSW	17	23,529,931 (GRCm39)	missense	probably benign	0.34
Z1088:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- TACTTCATCATTCTTTGGGAGGCTG -3'
(R):5'- CAGTGTCTGGAGGACCAATATGC -3'

Sequencing Primer
(F):5'- AGGCTGTGATTTTGAATGCCAGAAC -3'
(R):5'- TGTCTGGAGGACCAATATGCTAACAC -3'

Posted On

2016-07-06