|Institutional Source||Beutler Lab|
|Gene Name||guanine nucleotide binding protein (G protein), alpha inhibiting 1|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R5190 (G1)|
|Chromosomal Location||18265135-18360355 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 18291598 bp|
|Amino Acid Change||Valine to Glutamic Acid at position 109 (V109E)|
|Ref Sequence||ENSEMBL: ENSMUSP00000074259 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000074694]|
|Predicted Effect||probably benign
AA Change: V109E
PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
AA Change: V109E
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
PHENOTYPE: Mice homozygous for disruptions in this gene exhibit long term memory defects. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Gnai1||
(F):5'- TGGTCTTCATGATAGCTGACTTC -3'
(R):5'- ATCAAAGCCGTGCTGTTCTC -3'
(F):5'- ATAGCTGACTTCTAAAGCTGTGGC -3'
(R):5'- TGGTTAGCAAACACTTCCTCAG -3'