Incidental Mutation 'R5190:Zfp296'
ID 398046
Institutional Source Beutler Lab
Gene Symbol Zfp296
Ensembl Gene ENSMUSG00000011267
Gene Name zinc finger protein 296
Synonyms 2210018A16Rik
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.370) question?
Stock # R5190 (G1)
Quality Score 175
Status Not validated
Chromosome 7
Chromosomal Location 19311212-19314581 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 19311332 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Alanine at position 9 (V9A)
Ref Sequence ENSEMBL: ENSMUSP00000104093 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000051364] [ENSMUST00000086041] [ENSMUST00000108453] [ENSMUST00000117222] [ENSMUST00000119912] [ENSMUST00000122055] [ENSMUST00000122127] [ENSMUST00000207907] [ENSMUST00000207663] [ENSMUST00000208068] [ENSMUST00000208826] [ENSMUST00000151646]
AlphaFold E9Q6W4
Predicted Effect probably benign
Transcript: ENSMUST00000051364
SMART Domains Protein: ENSMUSP00000055844
Gene: ENSMUSG00000044709

DomainStartEndE-ValueType
low complexity region 4 20 N/A INTRINSIC
Pfam:Gemin7 50 129 1.9e-45 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000086041
SMART Domains Protein: ENSMUSP00000083205
Gene: ENSMUSG00000061028

DomainStartEndE-ValueType
low complexity region 19 35 N/A INTRINSIC
DRY_EERY 39 171 1.28e-64 SMART
low complexity region 172 212 N/A INTRINSIC
low complexity region 241 260 N/A INTRINSIC
low complexity region 263 283 N/A INTRINSIC
low complexity region 302 324 N/A INTRINSIC
low complexity region 339 368 N/A INTRINSIC
low complexity region 372 446 N/A INTRINSIC
low complexity region 453 476 N/A INTRINSIC
low complexity region 480 532 N/A INTRINSIC
coiled coil region 574 630 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000108453
AA Change: V9A

PolyPhen 2 Score 0.326 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000104093
Gene: ENSMUSG00000011267
AA Change: V9A

DomainStartEndE-ValueType
ZnF_C2H2 138 161 5.07e0 SMART
ZnF_C2H2 212 234 2.05e-2 SMART
ZnF_C2H2 240 262 1.1e-2 SMART
low complexity region 281 295 N/A INTRINSIC
low complexity region 317 329 N/A INTRINSIC
ZnF_C2H2 360 382 1.95e-3 SMART
ZnF_C2H2 388 410 3.39e-3 SMART
ZnF_C2H2 418 441 1.14e0 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000117222
SMART Domains Protein: ENSMUSP00000113266
Gene: ENSMUSG00000044709

DomainStartEndE-ValueType
low complexity region 4 20 N/A INTRINSIC
Pfam:Gemin7 50 129 1.9e-45 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000119912
SMART Domains Protein: ENSMUSP00000112742
Gene: ENSMUSG00000044709

DomainStartEndE-ValueType
low complexity region 4 20 N/A INTRINSIC
Pfam:Gemin7 50 129 1.9e-45 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000122055
SMART Domains Protein: ENSMUSP00000113583
Gene: ENSMUSG00000044709

DomainStartEndE-ValueType
low complexity region 4 20 N/A INTRINSIC
Pfam:Gemin7 50 129 1.9e-45 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000122127
SMART Domains Protein: ENSMUSP00000113709
Gene: ENSMUSG00000044709

DomainStartEndE-ValueType
low complexity region 4 20 N/A INTRINSIC
Pfam:Gemin7 50 129 1.9e-45 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000208464
Predicted Effect probably benign
Transcript: ENSMUST00000207907
Predicted Effect probably benign
Transcript: ENSMUST00000207663
Predicted Effect noncoding transcript
Transcript: ENSMUST00000208314
Predicted Effect noncoding transcript
Transcript: ENSMUST00000207253
Predicted Effect probably benign
Transcript: ENSMUST00000208068
Predicted Effect probably benign
Transcript: ENSMUST00000208826
Predicted Effect noncoding transcript
Transcript: ENSMUST00000208427
Predicted Effect noncoding transcript
Transcript: ENSMUST00000208716
Predicted Effect probably benign
Transcript: ENSMUST00000151646
SMART Domains Protein: ENSMUSP00000116207
Gene: ENSMUSG00000044709

DomainStartEndE-ValueType
low complexity region 4 20 N/A INTRINSIC
Pfam:Gemin7 50 81 7e-16 PFAM
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2700049A03Rik G T 12: 71,211,320 (GRCm39) E685* probably null Het
2700049A03Rik A T 12: 71,211,321 (GRCm39) E685V possibly damaging Het
Abca7 T C 10: 79,835,427 (GRCm39) probably null Het
Abca8a C T 11: 109,980,735 (GRCm39) probably null Het
Ablim3 A G 18: 61,952,982 (GRCm39) Y361H probably benign Het
Acan C T 7: 78,748,289 (GRCm39) T1020I probably benign Het
Baat A G 4: 49,499,652 (GRCm39) L218P probably damaging Het
Bcl11b C A 12: 107,955,975 (GRCm39) C58F probably damaging Het
Cand2 G T 6: 115,766,474 (GRCm39) A360S probably damaging Het
Cdh2 A G 18: 16,783,372 (GRCm39) V62A possibly damaging Het
Cep44 C T 8: 56,985,831 (GRCm39) V354I probably benign Het
Col3a1 G A 1: 45,368,244 (GRCm39) R309Q unknown Het
Col3a1 G A 1: 45,383,967 (GRCm39) probably benign Het
Coq5 A G 5: 115,433,839 (GRCm39) probably null Het
Crtac1 A G 19: 42,322,347 (GRCm39) I131T possibly damaging Het
Decr1 C T 4: 15,924,270 (GRCm39) V217M probably damaging Het
Dnajc13 A G 9: 104,051,724 (GRCm39) V1706A probably benign Het
Dop1a A G 9: 86,369,357 (GRCm39) I63M probably damaging Het
F830045P16Rik T C 2: 129,314,635 (GRCm39) D214G probably benign Het
Fam216a T A 5: 122,505,584 (GRCm39) probably null Het
Fdxacb1 A C 9: 50,683,387 (GRCm39) H248P possibly damaging Het
Gnai1 A T 5: 18,496,596 (GRCm39) V109E probably benign Het
Helz2 A T 2: 180,872,550 (GRCm39) probably null Het
Itgam T C 7: 127,715,489 (GRCm39) probably null Het
Kcnh1 A G 1: 192,187,836 (GRCm39) S766G probably benign Het
Klra1 C A 6: 130,352,241 (GRCm39) C167F probably damaging Het
Krtap9-3 T A 11: 99,488,808 (GRCm39) T25S probably benign Het
Mapk6 T C 9: 75,295,626 (GRCm39) Y624C probably damaging Het
Or10ag2 A G 2: 87,249,187 (GRCm39) Y263C probably damaging Het
Or1ad8 T G 11: 50,898,381 (GRCm39) I194S probably damaging Het
Or2y1g T C 11: 49,171,209 (GRCm39) V78A probably damaging Het
Or52e4 C T 7: 104,705,660 (GRCm39) S69L probably damaging Het
Or9m2 T A 2: 87,821,107 (GRCm39) Y217* probably null Het
P3h2 A G 16: 25,803,699 (GRCm39) S356P possibly damaging Het
Pde12 C T 14: 26,387,532 (GRCm39) probably null Het
Rln3 T G 8: 84,769,866 (GRCm39) K94N probably damaging Het
Skint5 T C 4: 113,620,711 (GRCm39) I668V unknown Het
Slitrk5 C A 14: 111,916,852 (GRCm39) Q159K probably damaging Het
Trim3 G T 7: 105,268,716 (GRCm39) N79K probably damaging Het
Tyw1 T A 5: 130,296,756 (GRCm39) C101* probably null Het
Ulk2 T C 11: 61,672,537 (GRCm39) T934A probably benign Het
Unc5b T C 10: 60,608,072 (GRCm39) Y687C probably benign Het
Vmn1r195 C T 13: 22,462,556 (GRCm39) R9* probably null Het
Zfc3h1 T G 10: 115,254,597 (GRCm39) L1397R probably damaging Het
Zfp423 T A 8: 88,509,091 (GRCm39) S397C probably damaging Het
Other mutations in Zfp296
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00590:Zfp296 APN 7 19,311,760 (GRCm39) missense possibly damaging 0.85
IGL01098:Zfp296 APN 7 19,311,845 (GRCm39) missense possibly damaging 0.72
R0359:Zfp296 UTSW 7 19,313,864 (GRCm39) missense possibly damaging 0.68
R0510:Zfp296 UTSW 7 19,311,831 (GRCm39) missense probably benign 0.01
R0707:Zfp296 UTSW 7 19,313,661 (GRCm39) missense probably benign 0.00
R4931:Zfp296 UTSW 7 19,313,637 (GRCm39) missense possibly damaging 0.73
R4932:Zfp296 UTSW 7 19,313,637 (GRCm39) missense possibly damaging 0.73
R4933:Zfp296 UTSW 7 19,313,637 (GRCm39) missense possibly damaging 0.73
R5974:Zfp296 UTSW 7 19,311,862 (GRCm39) missense probably benign 0.00
R9694:Zfp296 UTSW 7 19,314,227 (GRCm39) missense possibly damaging 0.71
Predicted Primers PCR Primer
(F):5'- TCGAGTTTGACCTCCGAGAC -3'
(R):5'- TTAGAACTCAGAGGCATCCGC -3'

Sequencing Primer
(F):5'- GAGACTGGCCCCGTAAAGAC -3'
(R):5'- CATCCGATATGGGCATGT -3'
Posted On 2016-07-06