Mutagenetix > Incidental Mutation

Incidental Mutation 'R5249:Pum1'

398760

Institutional Source

Beutler Lab

Gene Symbol

Pum1

Ensembl Gene

ENSMUSG00000028580

Gene Name

pumilio RNA-binding family member 1

Synonyms

Pumm

MMRRC Submission

042820-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.890) question?

Stock #

R5249 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130390632-130508875 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 130490125 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 699 (Y699F)

Ref Sequence

ENSEMBL: ENSMUSP00000101613 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030315] [ENSMUST00000097862] [ENSMUST00000097864] [ENSMUST00000105991] [ENSMUST00000105992]

AlphaFold

Q80U78

Predicted Effect

probably benign
Transcript: ENSMUST00000030315
AA Change: Y796F

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000030315
Gene: ENSMUSG00000028580
AA Change: Y796F

Domain	Start	End	E-Value	Type
low complexity region	45	58	N/A	INTRINSIC
low complexity region	94	102	N/A	INTRINSIC
low complexity region	393	414	N/A	INTRINSIC
low complexity region	443	458	N/A	INTRINSIC
low complexity region	476	503	N/A	INTRINSIC
low complexity region	528	539	N/A	INTRINSIC
low complexity region	584	615	N/A	INTRINSIC
low complexity region	627	637	N/A	INTRINSIC
low complexity region	643	666	N/A	INTRINSIC
low complexity region	672	696	N/A	INTRINSIC
low complexity region	731	741	N/A	INTRINSIC
low complexity region	763	783	N/A	INTRINSIC
low complexity region	798	816	N/A	INTRINSIC
Pumilio	849	884	1.75e-6	SMART
Pumilio	885	920	4.03e-6	SMART
Pumilio	921	955	5.24e-5	SMART
Pumilio	959	994	3.37e-8	SMART
Pumilio	995	1030	6.29e-8	SMART
Pumilio	1031	1066	1.04e-8	SMART
Pumilio	1067	1102	6.2e-7	SMART
Pumilio	1110	1145	8.77e-7	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000082550

Predicted Effect

probably benign
Transcript: ENSMUST00000097862
AA Change: Y795F

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000095474
Gene: ENSMUSG00000028580
AA Change: Y795F

Domain	Start	End	E-Value	Type
low complexity region	45	58	N/A	INTRINSIC
low complexity region	94	102	N/A	INTRINSIC
low complexity region	393	414	N/A	INTRINSIC
low complexity region	442	457	N/A	INTRINSIC
low complexity region	475	502	N/A	INTRINSIC
low complexity region	527	538	N/A	INTRINSIC
low complexity region	583	614	N/A	INTRINSIC
low complexity region	626	636	N/A	INTRINSIC
low complexity region	642	665	N/A	INTRINSIC
low complexity region	671	695	N/A	INTRINSIC
low complexity region	730	740	N/A	INTRINSIC
low complexity region	762	782	N/A	INTRINSIC
low complexity region	797	815	N/A	INTRINSIC
Pumilio	848	883	1.75e-6	SMART
Pumilio	884	919	4.03e-6	SMART
Pumilio	920	954	5.24e-5	SMART
Pumilio	958	993	3.37e-8	SMART
Pumilio	994	1029	6.29e-8	SMART
Pumilio	1030	1065	1.04e-8	SMART
Pumilio	1066	1101	6.2e-7	SMART
Pumilio	1109	1144	8.77e-7	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000097864
AA Change: Y795F

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000095476
Gene: ENSMUSG00000028580
AA Change: Y795F

Domain	Start	End	E-Value	Type
low complexity region	45	58	N/A	INTRINSIC
low complexity region	94	102	N/A	INTRINSIC
low complexity region	393	414	N/A	INTRINSIC
low complexity region	442	457	N/A	INTRINSIC
low complexity region	475	502	N/A	INTRINSIC
low complexity region	527	538	N/A	INTRINSIC
low complexity region	583	614	N/A	INTRINSIC
low complexity region	626	636	N/A	INTRINSIC
low complexity region	642	665	N/A	INTRINSIC
low complexity region	671	695	N/A	INTRINSIC
low complexity region	730	740	N/A	INTRINSIC
low complexity region	762	782	N/A	INTRINSIC
low complexity region	797	815	N/A	INTRINSIC
Pumilio	848	883	1.75e-6	SMART
Pumilio	884	919	4.03e-6	SMART
Pumilio	920	955	5.48e-8	SMART
Pumilio	956	991	3.37e-8	SMART
Pumilio	992	1027	6.29e-8	SMART
Pumilio	1028	1063	1.04e-8	SMART
Pumilio	1064	1099	6.2e-7	SMART
Pumilio	1107	1142	8.77e-7	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000105991
AA Change: Y553F

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000101612
Gene: ENSMUSG00000028580
AA Change: Y553F

Domain	Start	End	E-Value	Type
low complexity region	45	58	N/A	INTRINSIC
low complexity region	94	102	N/A	INTRINSIC
low complexity region	151	172	N/A	INTRINSIC
low complexity region	200	215	N/A	INTRINSIC
low complexity region	233	260	N/A	INTRINSIC
low complexity region	285	296	N/A	INTRINSIC
low complexity region	341	372	N/A	INTRINSIC
low complexity region	384	394	N/A	INTRINSIC
low complexity region	400	423	N/A	INTRINSIC
low complexity region	429	453	N/A	INTRINSIC
low complexity region	488	498	N/A	INTRINSIC
low complexity region	520	540	N/A	INTRINSIC
low complexity region	555	573	N/A	INTRINSIC
Pumilio	606	641	1.75e-6	SMART
Pumilio	642	677	4.03e-6	SMART
Pumilio	678	713	5.48e-8	SMART
Pumilio	714	749	3.37e-8	SMART
Pumilio	750	785	6.29e-8	SMART
Pumilio	786	821	1.04e-8	SMART
Pumilio	822	857	6.2e-7	SMART
Pumilio	865	900	8.77e-7	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000105992
AA Change: Y699F

PolyPhen 2 Score 0.070 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000101613
Gene: ENSMUSG00000028580
AA Change: Y699F

Domain	Start	End	E-Value	Type
low complexity region	45	58	N/A	INTRINSIC
low complexity region	94	102	N/A	INTRINSIC
low complexity region	297	318	N/A	INTRINSIC
low complexity region	346	361	N/A	INTRINSIC
low complexity region	379	406	N/A	INTRINSIC
low complexity region	431	442	N/A	INTRINSIC
low complexity region	487	518	N/A	INTRINSIC
low complexity region	530	540	N/A	INTRINSIC
low complexity region	546	569	N/A	INTRINSIC
low complexity region	575	599	N/A	INTRINSIC
low complexity region	634	644	N/A	INTRINSIC
low complexity region	666	686	N/A	INTRINSIC
low complexity region	701	719	N/A	INTRINSIC
Pumilio	752	787	1.75e-6	SMART
Pumilio	788	823	4.03e-6	SMART
Pumilio	824	858	5.24e-5	SMART
Pumilio	862	897	3.37e-8	SMART
Pumilio	898	933	6.29e-8	SMART
Pumilio	934	969	1.04e-8	SMART
Pumilio	970	1005	6.2e-7	SMART
Pumilio	1013	1048	8.77e-7	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154720

Meta Mutation Damage Score

0.1124

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

98% (93/95)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit decreased testes weight and size, decreased body weight, oligozoospermia, reduced male fertility, increased male germ cell apoptosis and small seminiferous tubules. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4gnt	A	G	9: 99,502,284 (GRCm39)	H148R	probably damaging	Het
Acrbp	A	G	6: 125,037,885 (GRCm39)	D394G	probably damaging	Het
Adam18	T	C	8: 25,115,868 (GRCm39)	T609A	probably benign	Het
Adam25	T	A	8: 41,208,991 (GRCm39)	N752K	probably benign	Het
Ano6	A	C	15: 95,811,469 (GRCm39)	S176R	probably benign	Het
Ano7	T	C	1: 93,302,918 (GRCm39)	S19P	probably benign	Het
Ap1b1	A	G	11: 4,976,364 (GRCm39)	E437G	probably damaging	Het
Arfgap2	T	A	2: 91,095,982 (GRCm39)	C46*	probably null	Het
Arhgef26	T	A	3: 62,247,981 (GRCm39)	L355Q	probably damaging	Het
B4galnt4	C	T	7: 140,644,983 (GRCm39)	T219I	probably damaging	Het
Bap1	C	T	14: 30,979,243 (GRCm39)		probably benign	Het
Catip	T	G	1: 74,401,954 (GRCm39)	L43R	probably damaging	Het
Ccdc177	G	A	12: 80,805,282 (GRCm39)	R331C	unknown	Het
Cep85l	T	C	10: 53,195,690 (GRCm39)		probably null	Het
Cfap206	T	A	4: 34,714,502 (GRCm39)	Q398L	probably benign	Het
Clec18a	A	G	8: 111,800,368 (GRCm39)	C352R	probably damaging	Het
Clic6	A	T	16: 92,336,339 (GRCm39)	Y549F	probably damaging	Het
Cpne9	C	T	6: 113,270,034 (GRCm39)		probably benign	Het
Cyb5r3	A	C	15: 83,042,836 (GRCm39)		probably benign	Het
Dnah6	C	T	6: 73,090,471 (GRCm39)	R2293K	probably damaging	Het
Dnmbp	C	A	19: 43,890,879 (GRCm39)	R296L	probably damaging	Het
Dtwd1	C	A	2: 125,996,694 (GRCm39)	Q60K	probably benign	Het
Esyt1	C	A	10: 128,352,443 (GRCm39)	V723L	probably benign	Het
Fbxo42	T	C	4: 140,926,335 (GRCm39)	L339P	probably damaging	Het
Furin	A	T	7: 80,043,169 (GRCm39)	N347K	probably damaging	Het
Fzd7	T	C	1: 59,522,522 (GRCm39)	M135T	probably damaging	Het
Garin1b	A	G	6: 29,323,896 (GRCm39)	D207G	probably damaging	Het
Hlf	A	G	11: 90,278,632 (GRCm39)	M144T	probably benign	Het
Hyal5	C	T	6: 24,876,648 (GRCm39)	Q174*	probably null	Het
Kcnb1	T	A	2: 166,947,103 (GRCm39)	M582L	possibly damaging	Het
Kdm5d	T	C	Y: 916,692 (GRCm39)	Y391H	probably damaging	Het
Khdc4	T	A	3: 88,604,032 (GRCm39)	I283N	probably damaging	Het
Kif21b	C	T	1: 136,096,966 (GRCm39)	T1297M	probably damaging	Het
Krt8	T	G	15: 101,906,875 (GRCm39)	N317T	possibly damaging	Het
Lctl	G	T	9: 64,045,196 (GRCm39)	V372L	probably benign	Het
Lig1	C	T	7: 13,042,432 (GRCm39)	H822Y	possibly damaging	Het
Lrrc4b	A	G	7: 44,111,988 (GRCm39)	D620G	possibly damaging	Het
Mboat4	C	T	8: 34,582,275 (GRCm39)	H10Y	probably benign	Het
Med1	A	T	11: 98,048,066 (GRCm39)	M910K	probably benign	Het
Mia2	A	T	12: 59,154,911 (GRCm39)	D209V	probably damaging	Het
Mpp4	C	T	1: 59,184,017 (GRCm39)		probably benign	Het
Mrgprb1	A	T	7: 48,097,225 (GRCm39)	V229E	possibly damaging	Het
Mtor	T	A	4: 148,548,189 (GRCm39)	C485S	probably damaging	Het
Mx1	T	C	16: 97,258,628 (GRCm39)	D23G	probably damaging	Het
Myrfl	T	C	10: 116,619,138 (GRCm39)	D740G	probably benign	Het
Myt1l	G	A	12: 29,882,331 (GRCm39)	G509R	unknown	Het
Nav2	G	A	7: 49,185,661 (GRCm39)	V874I	probably damaging	Het
Ntrk3	A	T	7: 78,110,914 (GRCm39)	N262K	possibly damaging	Het
Opa1	A	T	16: 29,437,077 (GRCm39)	D699V	probably damaging	Het
Or10u4	C	T	10: 129,802,078 (GRCm39)	A164T	probably benign	Het
Or8b51	A	G	9: 38,569,374 (GRCm39)	C105R	possibly damaging	Het
Otud7a	A	G	7: 63,407,181 (GRCm39)	N495D	possibly damaging	Het
Pcsk9	T	C	4: 106,320,950 (GRCm39)	D53G	probably benign	Het
Pdcd5	A	G	7: 35,346,421 (GRCm39)		probably benign	Het
Phf21a	C	T	2: 92,058,822 (GRCm39)	P28L	probably damaging	Het
Pitpnm1	T	A	19: 4,158,130 (GRCm39)	D573E	probably damaging	Het
Ppp1r35	C	T	5: 137,777,406 (GRCm39)		probably benign	Het
Ptprq	T	C	10: 107,535,496 (GRCm39)	Q423R	probably damaging	Het
Rabgef1	A	G	5: 130,241,841 (GRCm39)	D415G	probably benign	Het
Rapgef6	A	G	11: 54,413,943 (GRCm39)	E23G	probably benign	Het
Rpia	G	T	6: 70,760,563 (GRCm39)	C121*	probably null	Het
Sanbr	A	G	11: 23,525,483 (GRCm39)	*719Q	probably null	Het
Serpinb13	T	A	1: 106,926,427 (GRCm39)	W201R	probably damaging	Het
Sh3bp4	T	C	1: 89,065,456 (GRCm39)	C17R	probably damaging	Het
Sh3d21	A	G	4: 126,055,858 (GRCm39)		probably benign	Het
Slamf6	A	C	1: 171,764,249 (GRCm39)	N214T	probably damaging	Het
Slc7a2	T	C	8: 41,361,130 (GRCm39)	Y365H	possibly damaging	Het
Sox18	T	C	2: 181,312,971 (GRCm39)		probably null	Het
Spink7	A	T	18: 62,725,507 (GRCm39)	F79I	possibly damaging	Het
Srpk3	C	T	X: 72,818,555 (GRCm39)	R82*	probably null	Het
Sspo	A	T	6: 48,470,244 (GRCm39)	H4561L	probably damaging	Het
Tcaf1	T	C	6: 42,653,793 (GRCm39)	K700R	probably benign	Het
Tcf25	G	T	8: 124,115,372 (GRCm39)	R203L	probably damaging	Het
Tcp11	T	C	17: 28,290,757 (GRCm39)	I201V	possibly damaging	Het
Tex2	T	C	11: 106,437,615 (GRCm39)	D685G	unknown	Het
Themis	G	T	10: 28,637,195 (GRCm39)	E100*	probably null	Het
Tmem52b	G	A	6: 129,491,221 (GRCm39)		probably null	Het
Tnr	C	T	1: 159,512,226 (GRCm39)		probably benign	Het
Tox3	T	C	8: 90,975,444 (GRCm39)	I396V	probably benign	Het
Tpm2	T	A	4: 43,514,828 (GRCm39)	E269V	probably benign	Het
Tulp1	C	T	17: 28,581,651 (GRCm39)		probably benign	Het
Tymp	GC	GCC	15: 89,258,567 (GRCm39)		probably null	Het
Ube2j2	A	C	4: 156,033,515 (GRCm39)	K30Q	possibly damaging	Het
Usp19	T	A	9: 108,369,807 (GRCm39)	M1K	probably null	Het
Vmn2r52	C	A	7: 9,910,197 (GRCm39)	R6L	probably benign	Het
Zbed4	G	A	15: 88,665,290 (GRCm39)	V453M	probably benign	Het
Zfp319	T	C	8: 96,055,099 (GRCm39)	E368G	probably benign	Het
Zfp366	A	C	13: 99,366,117 (GRCm39)	E426A	probably damaging	Het
Zfp687	A	T	3: 94,916,777 (GRCm39)	I783N	probably damaging	Het

Other mutations in Pum1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00985:Pum1	APN	4	130,471,100 (GRCm39)	missense	probably damaging	1.00
IGL01327:Pum1	APN	4	130,457,854 (GRCm39)	missense	probably damaging	0.97
IGL01360:Pum1	APN	4	130,455,481 (GRCm39)	intron	probably benign
IGL02055:Pum1	APN	4	130,481,365 (GRCm39)	missense	probably benign	0.19
IGL02713:Pum1	APN	4	130,493,323 (GRCm39)	missense	probably damaging	1.00
IGL03401:Pum1	APN	4	130,470,992 (GRCm39)	splice site	probably benign
LCD18:Pum1	UTSW	4	130,730,549 (GRCm38)	intron	probably benign
R0077:Pum1	UTSW	4	130,499,985 (GRCm39)	missense	probably benign	0.06
R0346:Pum1	UTSW	4	130,507,116 (GRCm39)	missense	possibly damaging	0.74
R0632:Pum1	UTSW	4	130,455,415 (GRCm39)	missense	probably benign	0.34
R0870:Pum1	UTSW	4	130,496,155 (GRCm39)	missense	probably damaging	0.99
R1006:Pum1	UTSW	4	130,499,199 (GRCm39)	missense	probably damaging	0.98
R1300:Pum1	UTSW	4	130,493,272 (GRCm39)	missense	probably damaging	1.00
R1499:Pum1	UTSW	4	130,446,567 (GRCm39)	missense	probably damaging	1.00
R1572:Pum1	UTSW	4	130,445,515 (GRCm39)	missense	probably damaging	0.99
R1835:Pum1	UTSW	4	130,428,359 (GRCm39)	missense	possibly damaging	0.93
R1864:Pum1	UTSW	4	130,478,836 (GRCm39)	missense	possibly damaging	0.90
R1991:Pum1	UTSW	4	130,445,529 (GRCm39)	missense	possibly damaging	0.93
R2068:Pum1	UTSW	4	130,501,745 (GRCm39)	missense	probably benign	0.02
R2119:Pum1	UTSW	4	130,396,581 (GRCm39)	missense	possibly damaging	0.92
R2120:Pum1	UTSW	4	130,396,581 (GRCm39)	missense	possibly damaging	0.92
R2122:Pum1	UTSW	4	130,396,581 (GRCm39)	missense	possibly damaging	0.92
R2153:Pum1	UTSW	4	130,478,802 (GRCm39)	missense	probably damaging	1.00
R2164:Pum1	UTSW	4	130,455,395 (GRCm39)	missense	probably damaging	0.99
R2164:Pum1	UTSW	4	130,455,394 (GRCm39)	nonsense	probably null
R2280:Pum1	UTSW	4	130,493,322 (GRCm39)	missense	probably damaging	1.00
R3116:Pum1	UTSW	4	130,499,971 (GRCm39)	missense	probably damaging	1.00
R3890:Pum1	UTSW	4	130,491,393 (GRCm39)	missense	probably damaging	1.00
R3891:Pum1	UTSW	4	130,491,393 (GRCm39)	missense	probably damaging	1.00
R3892:Pum1	UTSW	4	130,491,393 (GRCm39)	missense	probably damaging	1.00
R4134:Pum1	UTSW	4	130,491,380 (GRCm39)	missense	probably damaging	1.00
R4258:Pum1	UTSW	4	130,457,591 (GRCm39)	missense	probably damaging	1.00
R4731:Pum1	UTSW	4	130,445,504 (GRCm39)	missense	probably benign	0.00
R4732:Pum1	UTSW	4	130,445,504 (GRCm39)	missense	probably benign	0.00
R4733:Pum1	UTSW	4	130,445,504 (GRCm39)	missense	probably benign	0.00
R4973:Pum1	UTSW	4	130,396,448 (GRCm39)	missense	probably benign	0.27
R5198:Pum1	UTSW	4	130,507,190 (GRCm39)	nonsense	probably null
R5478:Pum1	UTSW	4	130,478,795 (GRCm39)	missense	possibly damaging	0.93
R5652:Pum1	UTSW	4	130,491,438 (GRCm39)	missense	possibly damaging	0.95
R5932:Pum1	UTSW	4	130,457,677 (GRCm39)	missense	probably benign	0.04
R6008:Pum1	UTSW	4	130,496,158 (GRCm39)	missense	probably damaging	1.00
R6112:Pum1	UTSW	4	130,457,591 (GRCm39)	missense	probably damaging	1.00
R6416:Pum1	UTSW	4	130,455,598 (GRCm39)	splice site	probably null
R6426:Pum1	UTSW	4	130,481,283 (GRCm39)	missense	probably damaging	1.00
R6431:Pum1	UTSW	4	130,501,816 (GRCm39)	missense	probably damaging	1.00
R7226:Pum1	UTSW	4	130,499,292 (GRCm39)	missense	probably damaging	1.00
R7273:Pum1	UTSW	4	130,478,791 (GRCm39)	missense	probably damaging	0.99
R7423:Pum1	UTSW	4	130,501,856 (GRCm39)	missense	probably damaging	1.00
R7491:Pum1	UTSW	4	130,446,485 (GRCm39)	missense	probably benign	0.08
R7526:Pum1	UTSW	4	130,474,337 (GRCm39)	missense	probably damaging	0.99
R7731:Pum1	UTSW	4	130,490,274 (GRCm39)	missense	probably benign	0.29
R7911:Pum1	UTSW	4	130,501,788 (GRCm39)	missense	probably benign	0.40
R8065:Pum1	UTSW	4	130,478,836 (GRCm39)	missense	possibly damaging	0.90
R8067:Pum1	UTSW	4	130,478,836 (GRCm39)	missense	possibly damaging	0.90
R8305:Pum1	UTSW	4	130,499,231 (GRCm39)	missense	probably benign	0.02
R8476:Pum1	UTSW	4	130,480,024 (GRCm39)	missense	possibly damaging	0.91
R8835:Pum1	UTSW	4	130,471,064 (GRCm39)	missense	probably damaging	1.00
R8875:Pum1	UTSW	4	130,507,186 (GRCm39)	missense	possibly damaging	0.60
R9003:Pum1	UTSW	4	130,474,393 (GRCm39)	missense	probably benign	0.00
R9072:Pum1	UTSW	4	130,480,172 (GRCm39)	missense	probably damaging	1.00
R9073:Pum1	UTSW	4	130,480,172 (GRCm39)	missense	probably damaging	1.00
R9332:Pum1	UTSW	4	130,499,209 (GRCm39)	nonsense	probably null
R9496:Pum1	UTSW	4	130,446,664 (GRCm39)	critical splice donor site	probably null
R9801:Pum1	UTSW	4	130,481,328 (GRCm39)	missense	probably benign	0.28
X0024:Pum1	UTSW	4	130,507,101 (GRCm39)	missense	probably benign	0.00
Z1177:Pum1	UTSW	4	130,478,790 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CCGATGATAGCTCAGTGACATAGG -3'
(R):5'- AGAGCCATGCTGGTCTTGAG -3'

Sequencing Primer
(F):5'- TGATAGCTCAGTGACATAGGAGGAG -3'
(R):5'- GCCATGCTGGTCTTGAGAAAATTCC -3'

Posted On

2016-07-06