Mutagenetix > Incidental Mutation

Incidental Mutation 'R5172:Atrnl1'

398861

Institutional Source

Beutler Lab

Gene Symbol

Atrnl1

Ensembl Gene

ENSMUSG00000054843

Gene Name

attractin like 1

Synonyms

Alp

MMRRC Submission

042752-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.182) question?

Stock #

R5172 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

57599466-58121775 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 57673945 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 593 (Y593*)

Ref Sequence

ENSEMBL: ENSMUSP00000076514 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077282]

AlphaFold

Q6A051

Predicted Effect

probably null
Transcript: ENSMUST00000077282
AA Change: Y593*

SMART Domains

Protein: ENSMUSP00000076514
Gene: ENSMUSG00000054843
AA Change: Y593*

Domain	Start	End	E-Value	Type
low complexity region	25	32	N/A	INTRINSIC
EGF	61	90	5.71e-1	SMART
CUB	92	208	1.43e-11	SMART
EGF	209	244	1.95e1	SMART
Pfam:EGF_2	248	279	5.8e-7	PFAM
Pfam:Kelch_5	350	391	2.1e-9	PFAM
Pfam:Kelch_6	354	401	5.8e-8	PFAM
Pfam:Kelch_4	465	517	4.3e-7	PFAM
Pfam:Kelch_1	519	573	2.7e-6	PFAM
PSI	613	656	3.38e-1	SMART
PSI	665	708	2e-3	SMART
PSI	714	759	1.72e-2	SMART
CLECT	747	872	2.86e-20	SMART
PSI	888	938	6.26e-5	SMART
PSI	941	1011	1.73e-7	SMART
EGF_Lam	1013	1056	1.07e-5	SMART
low complexity region	1157	1173	N/A	INTRINSIC
transmembrane domain	1229	1251	N/A	INTRINSIC
low complexity region	1261	1272	N/A	INTRINSIC
low complexity region	1326	1339	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit normal coat coloring and normal brain morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc3	T	C	11: 94,266,434 (GRCm39)	Y52C	probably damaging	Het
Acmsd	C	T	1: 127,681,585 (GRCm39)	R183*	probably null	Het
Anxa2	T	A	9: 69,392,533 (GRCm39)	D127E	probably damaging	Het
Atxn2	C	T	5: 121,933,098 (GRCm39)		probably null	Het
Ccl6	A	T	11: 83,480,169 (GRCm39)	Y66N	probably damaging	Het
Ccng1	A	G	11: 40,642,113 (GRCm39)	V223A	probably benign	Het
Cfap44	T	C	16: 44,269,556 (GRCm39)	Y1187H	probably benign	Het
Cfc1	A	T	1: 34,575,011 (GRCm39)	I10F	probably benign	Het
Chrne	T	A	11: 70,506,352 (GRCm39)	T365S	probably benign	Het
Clec4b1	G	T	6: 123,048,414 (GRCm39)	R183L	probably benign	Het
Csmd2	A	C	4: 128,371,190 (GRCm39)	Q1926P	probably benign	Het
Dmpk	C	G	7: 18,821,944 (GRCm39)	L301V	probably benign	Het
Dzip1	T	A	14: 119,124,563 (GRCm39)	Q570L	probably damaging	Het
Fam149a	T	A	8: 45,797,690 (GRCm39)	Q507L	probably damaging	Het
Frem3	T	C	8: 81,339,195 (GRCm39)	V496A	probably benign	Het
Fryl	A	T	5: 73,259,016 (GRCm39)	D589E	possibly damaging	Het
Hemk1	T	C	9: 107,206,631 (GRCm39)	E4G	possibly damaging	Het
Igkv4-80	A	C	6: 68,993,649 (GRCm39)	S81A	probably benign	Het
Kcnh7	T	C	2: 62,569,508 (GRCm39)	D796G	possibly damaging	Het
Lemd2	G	T	17: 27,414,356 (GRCm39)	S326*	probably null	Het
Mdc1	T	C	17: 36,163,982 (GRCm39)	S1177P	probably benign	Het
Mfsd4b4	A	G	10: 39,770,083 (GRCm39)	F78S	probably damaging	Het
Mmgt2	T	A	11: 62,555,954 (GRCm39)	F101I	possibly damaging	Het
Myo18a	T	C	11: 77,714,924 (GRCm39)	L785P	probably damaging	Het
Nup155	C	A	15: 8,139,026 (GRCm39)	Q33K	probably benign	Het
Or52r1c	T	C	7: 102,734,884 (GRCm39)	L48P	probably damaging	Het
Or5w19	A	G	2: 87,699,171 (GRCm39)	T279A	probably benign	Het
Otx1	C	A	11: 21,947,037 (GRCm39)	A91S	probably damaging	Het
Pank1	A	T	19: 34,818,202 (GRCm39)	C112*	probably null	Het
Pcmtd1	T	A	1: 7,233,485 (GRCm39)	M23K	probably benign	Het
Rere	A	T	4: 150,654,726 (GRCm39)	R419S	unknown	Het
Rpf1	T	C	3: 146,218,050 (GRCm39)	R155G	possibly damaging	Het
Sema7a	A	G	9: 57,864,961 (GRCm39)	T421A	probably benign	Het
Sharpin	A	G	15: 76,231,741 (GRCm39)	S323P	probably benign	Het
Slamf6	A	G	1: 171,764,147 (GRCm39)	E180G	probably benign	Het
Snd1	T	G	6: 28,886,615 (GRCm39)	V874G	possibly damaging	Het
Sult6b2	A	T	6: 142,743,657 (GRCm39)	V123D	probably damaging	Het
Tpk1	A	T	6: 43,536,951 (GRCm39)		probably null	Het
Vmn1r160	A	T	7: 22,570,761 (GRCm39)	N38I	probably damaging	Het
Wdr93	T	A	7: 79,402,241 (GRCm39)	I180N	probably damaging	Het
Ythdf3	C	T	3: 16,258,198 (GRCm39)	T119I	probably damaging	Het
Zc3h18	T	G	8: 123,134,159 (GRCm39)		probably benign	Het

Other mutations in Atrnl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Atrnl1	APN	19	57,680,249 (GRCm39)	missense	probably benign	0.02
IGL00707:Atrnl1	APN	19	57,661,697 (GRCm39)	missense	probably damaging	0.96
IGL00921:Atrnl1	APN	19	57,690,585 (GRCm39)	missense	probably damaging	1.00
IGL01410:Atrnl1	APN	19	58,119,536 (GRCm39)	missense	probably damaging	1.00
IGL01468:Atrnl1	APN	19	57,688,144 (GRCm39)	missense	probably benign	0.02
IGL01756:Atrnl1	APN	19	57,641,380 (GRCm39)	missense	probably benign
IGL01971:Atrnl1	APN	19	57,741,715 (GRCm39)	missense	probably damaging	1.00
IGL02019:Atrnl1	APN	19	57,680,195 (GRCm39)	splice site	probably benign
IGL02580:Atrnl1	APN	19	57,703,008 (GRCm39)	splice site	probably benign
IGL02649:Atrnl1	APN	19	57,638,873 (GRCm39)	splice site	probably benign
IGL02676:Atrnl1	APN	19	57,680,316 (GRCm39)	missense	probably damaging	1.00
IGL03276:Atrnl1	APN	19	57,641,359 (GRCm39)	missense	probably damaging	0.99
IGL03379:Atrnl1	APN	19	57,630,973 (GRCm39)	missense	probably benign	0.02
Magnetogorsk	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
polar	UTSW	19	57,641,382 (GRCm39)	missense	probably benign	0.00
PIT4812001:Atrnl1	UTSW	19	57,720,055 (GRCm39)	missense	probably benign	0.08
R0109:Atrnl1	UTSW	19	57,743,949 (GRCm39)	missense	possibly damaging	0.78
R0308:Atrnl1	UTSW	19	57,741,720 (GRCm39)	missense	probably benign	0.04
R0394:Atrnl1	UTSW	19	57,661,608 (GRCm39)	missense	probably benign	0.10
R0734:Atrnl1	UTSW	19	57,643,293 (GRCm39)	missense	probably damaging	1.00
R0811:Atrnl1	UTSW	19	57,661,573 (GRCm39)	missense	probably benign	0.07
R0812:Atrnl1	UTSW	19	57,661,573 (GRCm39)	missense	probably benign	0.07
R1183:Atrnl1	UTSW	19	57,638,725 (GRCm39)	missense	probably damaging	0.97
R1213:Atrnl1	UTSW	19	57,626,894 (GRCm39)	missense	probably benign	0.25
R1344:Atrnl1	UTSW	19	57,924,137 (GRCm39)	critical splice donor site	probably null
R1418:Atrnl1	UTSW	19	57,924,137 (GRCm39)	critical splice donor site	probably null
R1707:Atrnl1	UTSW	19	57,675,169 (GRCm39)	missense	probably benign	0.00
R1748:Atrnl1	UTSW	19	57,703,134 (GRCm39)	missense	probably damaging	0.99
R2051:Atrnl1	UTSW	19	57,680,281 (GRCm39)	missense	probably benign	0.01
R2113:Atrnl1	UTSW	19	57,744,048 (GRCm39)	nonsense	probably null
R2130:Atrnl1	UTSW	19	57,643,426 (GRCm39)	missense	probably damaging	1.00
R3710:Atrnl1	UTSW	19	57,645,546 (GRCm39)	missense	probably damaging	1.00
R3916:Atrnl1	UTSW	19	57,924,084 (GRCm39)	missense	possibly damaging	0.82
R4524:Atrnl1	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
R4707:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4712:Atrnl1	UTSW	19	57,641,382 (GRCm39)	missense	probably benign	0.00
R4784:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4785:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4798:Atrnl1	UTSW	19	58,030,793 (GRCm39)	missense	probably benign
R5226:Atrnl1	UTSW	19	57,638,767 (GRCm39)	missense	probably benign
R5289:Atrnl1	UTSW	19	57,645,514 (GRCm39)	missense	probably damaging	1.00
R5372:Atrnl1	UTSW	19	57,743,968 (GRCm39)	missense	probably benign
R5737:Atrnl1	UTSW	19	57,766,320 (GRCm39)	missense	possibly damaging	0.84
R5782:Atrnl1	UTSW	19	57,741,718 (GRCm39)	missense	possibly damaging	0.95
R5826:Atrnl1	UTSW	19	57,618,724 (GRCm39)	nonsense	probably null
R6169:Atrnl1	UTSW	19	57,630,895 (GRCm39)	missense	probably benign	0.00
R6242:Atrnl1	UTSW	19	57,630,910 (GRCm39)	missense	probably benign	0.02
R6342:Atrnl1	UTSW	19	57,626,942 (GRCm39)	missense	probably damaging	1.00
R6372:Atrnl1	UTSW	19	57,638,764 (GRCm39)	missense	probably benign	0.01
R6811:Atrnl1	UTSW	19	57,643,393 (GRCm39)	missense	probably damaging	0.98
R6897:Atrnl1	UTSW	19	58,030,800 (GRCm39)	missense	probably benign	0.01
R7024:Atrnl1	UTSW	19	57,626,882 (GRCm39)	critical splice acceptor site	probably null
R7085:Atrnl1	UTSW	19	57,680,289 (GRCm39)	missense	probably damaging	1.00
R7144:Atrnl1	UTSW	19	58,030,784 (GRCm39)	missense	probably damaging	1.00
R7259:Atrnl1	UTSW	19	57,924,038 (GRCm39)	nonsense	probably null
R7289:Atrnl1	UTSW	19	57,638,846 (GRCm39)	missense	probably benign	0.13
R7310:Atrnl1	UTSW	19	57,630,856 (GRCm39)	missense	possibly damaging	0.69
R7372:Atrnl1	UTSW	19	57,924,078 (GRCm39)	missense	possibly damaging	0.47
R7432:Atrnl1	UTSW	19	57,743,956 (GRCm39)	missense	probably damaging	1.00
R7478:Atrnl1	UTSW	19	57,684,744 (GRCm39)	missense	possibly damaging	0.89
R7556:Atrnl1	UTSW	19	57,643,278 (GRCm39)	missense	probably benign
R7567:Atrnl1	UTSW	19	57,687,955 (GRCm39)	missense	probably damaging	0.98
R7608:Atrnl1	UTSW	19	57,703,119 (GRCm39)	missense	probably damaging	1.00
R7632:Atrnl1	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
R7655:Atrnl1	UTSW	19	57,599,811 (GRCm39)	nonsense	probably null
R7656:Atrnl1	UTSW	19	57,599,811 (GRCm39)	nonsense	probably null
R7718:Atrnl1	UTSW	19	57,728,615 (GRCm39)	nonsense	probably null
R7721:Atrnl1	UTSW	19	57,684,763 (GRCm39)	missense	probably benign	0.00
R7726:Atrnl1	UTSW	19	57,690,504 (GRCm39)	missense	probably damaging	1.00
R7733:Atrnl1	UTSW	19	57,690,420 (GRCm39)	missense	probably benign	0.00
R7774:Atrnl1	UTSW	19	57,688,103 (GRCm39)	missense	probably damaging	1.00
R8010:Atrnl1	UTSW	19	57,670,878 (GRCm39)	missense	probably benign	0.14
R8119:Atrnl1	UTSW	19	57,630,895 (GRCm39)	missense	probably benign	0.00
R9242:Atrnl1	UTSW	19	57,645,660 (GRCm39)	missense	probably benign	0.07
R9265:Atrnl1	UTSW	19	57,766,359 (GRCm39)	missense	probably benign	0.11
R9272:Atrnl1	UTSW	19	57,643,420 (GRCm39)	missense	probably benign	0.00
R9480:Atrnl1	UTSW	19	57,690,420 (GRCm39)	missense	possibly damaging	0.61
R9526:Atrnl1	UTSW	19	57,617,551 (GRCm39)	missense	probably damaging	0.99
R9672:Atrnl1	UTSW	19	57,618,695 (GRCm39)	missense	possibly damaging	0.87
R9673:Atrnl1	UTSW	19	57,599,786 (GRCm39)	start codon destroyed	probably null	0.04
RF021:Atrnl1	UTSW	19	57,630,905 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TCTAGACAGCTATTCTAAAGTGTACC -3'
(R):5'- ACCTCACCTGTCTTGGGAGG -3'

Sequencing Primer
(F):5'- GTTAATCTGGGCTTTTTACATCATGC -3'
(R):5'- GCACTTGGCTCTGAGAATATTATTTG -3'

Posted On

2016-07-06