Mutagenetix > Incidental Mutation

Incidental Mutation 'R5253:Otof'

399186

Institutional Source

Beutler Lab

Gene Symbol

Otof

Ensembl Gene

ENSMUSG00000062372

Gene Name

otoferlin

Synonyms

MMRRC Submission

042824-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.121) question?

Stock #

R5253 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

30367062-30461932 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 30370139 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 1985 (S1985P)

Ref Sequence

ENSEMBL: ENSMUSP00000073803 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074171] [ENSMUST00000101448] [ENSMUST00000114747]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000074171
AA Change: S1985P

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000073803
Gene: ENSMUSG00000062372
AA Change: S1985P

Domain	Start	End	E-Value	Type
C2	2	97	6.83e-1	SMART
C2	254	352	3.76e-11	SMART
FerI	338	409	7.91e-38	SMART
C2	417	528	1.75e-11	SMART
low complexity region	607	618	N/A	INTRINSIC
FerB	841	917	5.13e-46	SMART
C2	960	1067	1.77e-7	SMART
low complexity region	1191	1202	N/A	INTRINSIC
low complexity region	1265	1276	N/A	INTRINSIC
low complexity region	1293	1323	N/A	INTRINSIC
low complexity region	1370	1385	N/A	INTRINSIC
low complexity region	1436	1447	N/A	INTRINSIC
C2	1493	1592	6.54e-11	SMART
C2	1733	1863	4.02e0	SMART
Pfam:Ferlin_C	1895	1994	7.2e-33	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000101448

SMART Domains

Protein: ENSMUSP00000098992
Gene: ENSMUSG00000073102

Domain	Start	End	E-Value	Type
low complexity region	33	55	N/A	INTRINSIC
low complexity region	62	79	N/A	INTRINSIC
Pfam:NYD-SP28	100	200	1.7e-33	PFAM
coiled coil region	280	318	N/A	INTRINSIC
low complexity region	455	473	N/A	INTRINSIC
low complexity region	559	569	N/A	INTRINSIC
low complexity region	599	612	N/A	INTRINSIC
Pfam:NYD-SP28_assoc	673	732	2.2e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114747

SMART Domains

Protein: ENSMUSP00000110395
Gene: ENSMUSG00000062372

Domain	Start	End	E-Value	Type
C2	2	97	6.83e-1	SMART
C2	269	367	3.76e-11	SMART
FerI	353	424	7.91e-38	SMART
C2	432	543	1.75e-11	SMART
low complexity region	622	633	N/A	INTRINSIC
FerB	856	932	5.13e-46	SMART
C2	975	1082	1.77e-7	SMART
Pfam:C2	1153	1236	1.8e-1	PFAM
low complexity region	1260	1271	N/A	INTRINSIC
low complexity region	1288	1318	N/A	INTRINSIC
low complexity region	1365	1380	N/A	INTRINSIC
low complexity region	1431	1442	N/A	INTRINSIC
C2	1488	1587	6.54e-11	SMART
C2	1728	1858	4.02e0	SMART
low complexity region	1903	1915	N/A	INTRINSIC
transmembrane domain	1959	1981	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144125

SMART Domains

Protein: ENSMUSP00000120679
Gene: ENSMUSG00000062372

Domain	Start	End	E-Value	Type
C2	2	97	6.83e-1	SMART
C2	254	352	3.76e-11	SMART
FerI	338	409	7.91e-38	SMART
C2	417	528	1.75e-11	SMART
low complexity region	607	618	N/A	INTRINSIC
FerB	841	917	5.13e-46	SMART
C2	960	1067	1.77e-7	SMART
low complexity region	1191	1202	N/A	INTRINSIC
low complexity region	1265	1276	N/A	INTRINSIC
low complexity region	1293	1323	N/A	INTRINSIC
low complexity region	1370	1385	N/A	INTRINSIC
low complexity region	1436	1447	N/A	INTRINSIC
C2	1493	1592	6.54e-11	SMART
C2	1733	1863	4.02e0	SMART
Pfam:Ferlin_C	1895	1994	7.2e-33	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000150734

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200001

Meta Mutation Damage Score

0.1499

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.8%

Validation Efficiency

99% (72/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutants have no detectable auditory brainstem response at any frequency tested. Otoacoustic transmission distortion products are detected. Direct electrical stimulation of cochlear ganglia elicits brainstem responses. On depolarization, inner hair cells release almost no neurotransmitter. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001J11Rik	T	C	9: 40,051,450 (GRCm38)		noncoding transcript	Het
Actrt2	A	C	4: 154,667,569 (GRCm38)	S37A	possibly damaging	Het
Adcy7	T	C	8: 88,314,114 (GRCm38)	I327T	probably damaging	Het
Ankrd13b	A	G	11: 77,473,235 (GRCm38)		probably benign	Het
Arap1	A	C	7: 101,388,644 (GRCm38)	I237L	probably benign	Het
Arhgap17	A	T	7: 123,303,748 (GRCm38)	Y359N	probably benign	Het
Atad1	A	G	19: 32,674,302 (GRCm38)	M343T	probably benign	Het
Cacna1b	A	T	2: 24,719,952 (GRCm38)	I392N	probably damaging	Het
Cacna1c	A	G	6: 118,597,969 (GRCm38)	S1914P	probably benign	Het
Cd300a	G	T	11: 114,894,751 (GRCm38)	R174L	probably benign	Het
Dip2a	G	A	10: 76,299,997 (GRCm38)	P356L	probably damaging	Het
Dsg1c	T	C	18: 20,272,379 (GRCm38)	L283P	probably damaging	Het
Dusp1	T	C	17: 26,508,217 (GRCm38)	N36S	probably benign	Het
Ercc3	C	A	18: 32,269,864 (GRCm38)	P776Q	probably damaging	Het
Etv1	A	G	12: 38,852,249 (GRCm38)	R260G	possibly damaging	Het
Fa2h	C	G	8: 111,349,237 (GRCm38)	M251I	probably benign	Het
Flg2	A	G	3: 93,200,812 (GRCm38)	D49G	probably damaging	Het
Fras1	A	G	5: 96,741,025 (GRCm38)	E2810G	probably damaging	Het
Fuk	T	C	8: 110,883,867 (GRCm38)	E968G	possibly damaging	Het
Gabbr1	T	C	17: 37,055,913 (GRCm38)	F343S	possibly damaging	Het
Gdf2	A	G	14: 33,945,307 (GRCm38)	T329A	probably benign	Het
Hcn4	T	A	9: 58,824,275 (GRCm38)	I255N	unknown	Het
Hk3	T	G	13: 55,011,011 (GRCm38)	D485A	probably damaging	Het
Hook3	T	C	8: 26,072,291 (GRCm38)	T249A	probably benign	Het
Kcp	C	T	6: 29,498,520 (GRCm38)		probably benign	Het
Kifc2	G	T	15: 76,666,281 (GRCm38)	R515L	possibly damaging	Het
Kiss1r	A	G	10: 79,920,750 (GRCm38)	Y142C	probably damaging	Het
Klk14	G	A	7: 43,692,077 (GRCm38)	C51Y	probably damaging	Het
Klrb1a	T	A	6: 128,619,163 (GRCm38)	I72L	probably benign	Het
Lep	T	A	6: 29,070,863 (GRCm38)	F62Y	probably damaging	Het
Lrtm1	A	G	14: 29,021,844 (GRCm38)	T90A	probably benign	Het
Mug1	A	T	6: 121,888,913 (GRCm38)	D1472V	probably benign	Het
Ncor2	G	T	5: 125,026,930 (GRCm38)	P1988Q	probably benign	Het
Nlrp4a	C	T	7: 26,450,492 (GRCm38)	S508L	probably benign	Het
Obp2b	T	A	2: 25,737,143 (GRCm38)	D29E	probably benign	Het
Olfr1123	G	A	2: 87,418,668 (GRCm38)	V207M	possibly damaging	Het
Olfr1214	A	G	2: 88,988,100 (GRCm38)	L34P	possibly damaging	Het
Olfr1224-ps1	A	T	2: 89,156,457 (GRCm38)	C239*	probably null	Het
Olfr725	T	C	14: 50,035,288 (GRCm38)	I38M	possibly damaging	Het
Olfr803	A	G	10: 129,691,732 (GRCm38)	I103T	probably damaging	Het
Oxct2a	A	T	4: 123,323,093 (GRCm38)	V165E	probably damaging	Het
Papd5	C	A	8: 88,200,023 (GRCm38)	H20Q	possibly damaging	Het
Pcdhgb7	T	C	18: 37,753,097 (GRCm38)	V440A	possibly damaging	Het
Pelp1	C	A	11: 70,401,661 (GRCm38)	G211C	probably damaging	Het
Phox2a	A	G	7: 101,822,105 (GRCm38)	H268R	probably benign	Het
Pik3c2g	T	C	6: 139,896,257 (GRCm38)		probably null	Het
Pramel1	T	A	4: 143,398,586 (GRCm38)	M360K	probably benign	Het
Rbm6	C	T	9: 107,852,657 (GRCm38)	R132K	probably damaging	Het
Slc22a30	G	A	19: 8,344,393 (GRCm38)	Q436*	probably null	Het
Slc45a1	T	C	4: 150,638,270 (GRCm38)	T386A	probably damaging	Het
Smad2	A	G	18: 76,288,053 (GRCm38)	Y151C	probably damaging	Het
Sptbn2	G	A	19: 4,750,082 (GRCm38)	G2188D	probably benign	Het
Sugt1	G	A	14: 79,602,901 (GRCm38)		probably null	Het
Tctn3	T	C	19: 40,607,241 (GRCm38)	S367G	probably benign	Het
Tead1	G	T	7: 112,861,545 (GRCm38)	D219Y	probably damaging	Het
Tenm2	G	T	11: 36,047,201 (GRCm38)	Y1548*	probably null	Het
Tenm3	T	A	8: 48,229,198 (GRCm38)	I2466F	possibly damaging	Het
Tgm2	G	A	2: 158,129,438 (GRCm38)	P294S	probably damaging	Het
Tns2	T	C	15: 102,111,453 (GRCm38)	S585P	probably damaging	Het
Ttc41	A	G	10: 86,730,942 (GRCm38)	K491E	probably benign	Het
Ttn	G	A	2: 76,791,551 (GRCm38)	T15549I	probably damaging	Het
Vmn1r157	T	C	7: 22,761,758 (GRCm38)	L21P	probably damaging	Het
Vmn2r81	A	G	10: 79,247,986 (GRCm38)	M65V	probably benign	Het
Wdr34	T	A	2: 30,032,363 (GRCm38)		probably benign	Het
Zcchc14	T	C	8: 121,618,694 (GRCm38)		probably benign	Het

Other mutations in Otof

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00159:Otof	APN	5	30,375,904 (GRCm38)	missense	probably damaging	1.00
IGL00391:Otof	APN	5	30,375,623 (GRCm38)	missense	probably damaging	1.00
IGL00579:Otof	APN	5	30,399,322 (GRCm38)	missense	possibly damaging	0.88
IGL00671:Otof	APN	5	30,385,753 (GRCm38)	critical splice donor site	probably null
IGL01019:Otof	APN	5	30,405,216 (GRCm38)	missense	probably benign	0.01
IGL01025:Otof	APN	5	30,384,253 (GRCm38)	missense	possibly damaging	0.82
IGL01086:Otof	APN	5	30,376,273 (GRCm38)	critical splice donor site	probably null
IGL01110:Otof	APN	5	30,461,725 (GRCm38)	missense	probably damaging	1.00
IGL01160:Otof	APN	5	30,381,535 (GRCm38)	missense	probably benign	0.00
IGL01285:Otof	APN	5	30,405,183 (GRCm38)	missense	probably damaging	1.00
IGL01329:Otof	APN	5	30,441,379 (GRCm38)	missense	probably benign	0.00
IGL01337:Otof	APN	5	30,419,512 (GRCm38)	missense	probably benign	0.17
IGL01337:Otof	APN	5	30,405,777 (GRCm38)	missense	possibly damaging	0.93
IGL01834:Otof	APN	5	30,399,220 (GRCm38)	missense	probably damaging	1.00
IGL01872:Otof	APN	5	30,379,254 (GRCm38)	splice site	probably benign
IGL01969:Otof	APN	5	30,382,483 (GRCm38)	splice site	probably benign
IGL02075:Otof	APN	5	30,370,726 (GRCm38)	missense	probably benign	0.23
IGL02077:Otof	APN	5	30,399,235 (GRCm38)	missense	probably damaging	1.00
IGL02136:Otof	APN	5	30,373,992 (GRCm38)	missense	possibly damaging	0.90
IGL02227:Otof	APN	5	30,370,784 (GRCm38)	missense	probably damaging	1.00
IGL02475:Otof	APN	5	30,376,682 (GRCm38)	missense	probably damaging	1.00
IGL02812:Otof	APN	5	30,374,082 (GRCm38)	missense	probably benign	0.08
IGL02864:Otof	APN	5	30,386,341 (GRCm38)	missense	probably damaging	0.99
IGL03176:Otof	APN	5	30,405,176 (GRCm38)	splice site	probably null
R0285:Otof	UTSW	5	30,379,533 (GRCm38)	critical splice donor site	probably null
R0421:Otof	UTSW	5	30,371,568 (GRCm38)	missense	possibly damaging	0.94
R0570:Otof	UTSW	5	30,371,881 (GRCm38)	splice site	probably benign
R0599:Otof	UTSW	5	30,370,705 (GRCm38)	missense	probably damaging	1.00
R0675:Otof	UTSW	5	30,382,361 (GRCm38)	missense	probably benign	0.01
R0715:Otof	UTSW	5	30,394,697 (GRCm38)	missense	probably damaging	0.99
R1019:Otof	UTSW	5	30,370,743 (GRCm38)	missense	probably damaging	0.96
R1183:Otof	UTSW	5	30,371,912 (GRCm38)	missense	probably damaging	1.00
R1435:Otof	UTSW	5	30,378,695 (GRCm38)	missense	probably benign	0.00
R1469:Otof	UTSW	5	30,380,227 (GRCm38)	missense	probably benign	0.00
R1469:Otof	UTSW	5	30,380,227 (GRCm38)	missense	probably benign	0.00
R1474:Otof	UTSW	5	30,379,532 (GRCm38)	critical splice donor site	probably null
R1524:Otof	UTSW	5	30,379,556 (GRCm38)	missense	probably benign	0.03
R1563:Otof	UTSW	5	30,371,005 (GRCm38)	missense	probably benign	0.00
R1732:Otof	UTSW	5	30,386,471 (GRCm38)	missense	probably damaging	1.00
R1822:Otof	UTSW	5	30,378,710 (GRCm38)	missense	probably benign	0.00
R1845:Otof	UTSW	5	30,371,723 (GRCm38)	nonsense	probably null
R1925:Otof	UTSW	5	30,394,188 (GRCm38)	missense	probably benign	0.37
R1938:Otof	UTSW	5	30,376,369 (GRCm38)	missense	probably benign	0.00
R1968:Otof	UTSW	5	30,388,654 (GRCm38)	missense	probably damaging	1.00
R1996:Otof	UTSW	5	30,421,037 (GRCm38)	missense	probably benign	0.01
R1999:Otof	UTSW	5	30,388,772 (GRCm38)	missense	probably benign	0.19
R2027:Otof	UTSW	5	30,421,014 (GRCm38)	missense	probably benign	0.08
R2138:Otof	UTSW	5	30,461,770 (GRCm38)	missense	probably benign	0.01
R2173:Otof	UTSW	5	30,386,374 (GRCm38)	missense	probably damaging	1.00
R2245:Otof	UTSW	5	30,370,207 (GRCm38)	missense	probably damaging	1.00
R3011:Otof	UTSW	5	30,382,840 (GRCm38)	missense	probably damaging	1.00
R3105:Otof	UTSW	5	30,381,801 (GRCm38)	missense	probably benign	0.03
R3442:Otof	UTSW	5	30,371,689 (GRCm38)	missense	probably damaging	1.00
R3710:Otof	UTSW	5	30,385,266 (GRCm38)	missense	probably benign
R3715:Otof	UTSW	5	30,376,871 (GRCm38)	nonsense	probably null
R3806:Otof	UTSW	5	30,386,499 (GRCm38)	critical splice acceptor site	probably null
R3975:Otof	UTSW	5	30,370,712 (GRCm38)	missense	probably damaging	1.00
R4067:Otof	UTSW	5	30,399,291 (GRCm38)	missense	probably damaging	1.00
R4077:Otof	UTSW	5	30,419,506 (GRCm38)	missense	possibly damaging	0.89
R4166:Otof	UTSW	5	30,382,418 (GRCm38)	missense	probably damaging	1.00
R4451:Otof	UTSW	5	30,385,164 (GRCm38)	missense	possibly damaging	0.77
R4485:Otof	UTSW	5	30,375,000 (GRCm38)	missense	possibly damaging	0.77
R4600:Otof	UTSW	5	30,371,900 (GRCm38)	missense	probably damaging	1.00
R4646:Otof	UTSW	5	30,383,570 (GRCm38)	missense	possibly damaging	0.82
R4648:Otof	UTSW	5	30,383,570 (GRCm38)	missense	possibly damaging	0.82
R4669:Otof	UTSW	5	30,420,974 (GRCm38)	critical splice donor site	probably null
R4773:Otof	UTSW	5	30,394,682 (GRCm38)	missense	probably benign	0.05
R4839:Otof	UTSW	5	30,419,404 (GRCm38)	missense	probably damaging	0.99
R4907:Otof	UTSW	5	30,378,661 (GRCm38)	critical splice donor site	probably null
R4961:Otof	UTSW	5	30,383,493 (GRCm38)	intron	probably benign
R4991:Otof	UTSW	5	30,394,181 (GRCm38)	missense	probably damaging	1.00
R5015:Otof	UTSW	5	30,382,894 (GRCm38)	missense	probably damaging	1.00
R5036:Otof	UTSW	5	30,384,439 (GRCm38)	missense	possibly damaging	0.54
R5038:Otof	UTSW	5	30,384,439 (GRCm38)	missense	possibly damaging	0.54
R5336:Otof	UTSW	5	30,376,720 (GRCm38)	missense	probably benign	0.01
R5365:Otof	UTSW	5	30,381,800 (GRCm38)	missense	probably damaging	0.99
R5901:Otof	UTSW	5	30,374,979 (GRCm38)	missense	probably damaging	1.00
R6211:Otof	UTSW	5	30,371,900 (GRCm38)	missense	probably damaging	0.99
R6318:Otof	UTSW	5	30,414,544 (GRCm38)	missense	probably damaging	1.00
R6331:Otof	UTSW	5	30,371,935 (GRCm38)	missense	possibly damaging	0.94
R6671:Otof	UTSW	5	30,419,533 (GRCm38)	missense	probably benign
R6701:Otof	UTSW	5	30,370,797 (GRCm38)	nonsense	probably null
R6792:Otof	UTSW	5	30,375,634 (GRCm38)	missense	probably damaging	1.00
R6853:Otof	UTSW	5	30,388,239 (GRCm38)	missense	probably damaging	1.00
R6940:Otof	UTSW	5	30,371,643 (GRCm38)	missense	probably damaging	0.96
R7037:Otof	UTSW	5	30,381,538 (GRCm38)	missense	probably benign	0.32
R7060:Otof	UTSW	5	30,388,356 (GRCm38)	missense	possibly damaging	0.84
R7089:Otof	UTSW	5	30,371,568 (GRCm38)	missense	possibly damaging	0.94
R7165:Otof	UTSW	5	30,375,620 (GRCm38)	missense	probably damaging	0.99
R7178:Otof	UTSW	5	30,383,534 (GRCm38)	missense	possibly damaging	0.50
R7298:Otof	UTSW	5	30,388,270 (GRCm38)	missense	probably damaging	1.00
R7393:Otof	UTSW	5	30,370,270 (GRCm38)	missense	probably benign	0.45
R7397:Otof	UTSW	5	30,375,707 (GRCm38)	missense	probably damaging	1.00
R7400:Otof	UTSW	5	30,385,188 (GRCm38)	missense	probably benign	0.04
R7428:Otof	UTSW	5	30,389,825 (GRCm38)	missense	probably damaging	1.00
R7456:Otof	UTSW	5	30,394,661 (GRCm38)	missense	probably damaging	1.00
R7505:Otof	UTSW	5	30,371,020 (GRCm38)	missense	probably benign	0.00
R7714:Otof	UTSW	5	30,370,253 (GRCm38)	missense	probably damaging	0.99
R8002:Otof	UTSW	5	30,380,610 (GRCm38)	missense	probably benign	0.10
R8032:Otof	UTSW	5	30,461,798 (GRCm38)	start codon destroyed	probably benign	0.07
R8153:Otof	UTSW	5	30,388,735 (GRCm38)	missense	probably damaging	1.00
R8158:Otof	UTSW	5	30,380,194 (GRCm38)	missense	probably benign	0.37
R8159:Otof	UTSW	5	30,380,194 (GRCm38)	missense	probably benign	0.37
R8441:Otof	UTSW	5	30,380,856 (GRCm38)	missense	probably damaging	0.99
R8738:Otof	UTSW	5	30,388,624 (GRCm38)	nonsense	probably null
R8813:Otof	UTSW	5	30,382,898 (GRCm38)	missense	probably benign	0.02
R8835:Otof	UTSW	5	30,370,920 (GRCm38)	missense	probably benign	0.44
R8852:Otof	UTSW	5	30,371,700 (GRCm38)	missense	possibly damaging	0.94
R8869:Otof	UTSW	5	30,420,981 (GRCm38)	missense	probably benign	0.08
R9029:Otof	UTSW	5	30,370,075 (GRCm38)	critical splice donor site	probably null
R9031:Otof	UTSW	5	30,380,188 (GRCm38)	missense	probably benign
R9061:Otof	UTSW	5	30,388,657 (GRCm38)	missense	possibly damaging	0.50
R9100:Otof	UTSW	5	30,382,352 (GRCm38)	missense	possibly damaging	0.80
R9121:Otof	UTSW	5	30,379,118 (GRCm38)	missense	probably benign	0.04
R9188:Otof	UTSW	5	30,376,751 (GRCm38)	missense	probably damaging	1.00
R9218:Otof	UTSW	5	30,385,125 (GRCm38)	missense	probably benign
R9280:Otof	UTSW	5	30,371,550 (GRCm38)	missense	probably damaging	0.98
R9395:Otof	UTSW	5	30,375,632 (GRCm38)	missense	probably damaging	1.00
R9400:Otof	UTSW	5	30,383,519 (GRCm38)	critical splice donor site	probably null
R9407:Otof	UTSW	5	30,380,921 (GRCm38)	missense	probably damaging	1.00
R9616:Otof	UTSW	5	30,382,364 (GRCm38)	missense	possibly damaging	0.95
R9665:Otof	UTSW	5	30,427,551 (GRCm38)	missense	probably benign	0.22
R9748:Otof	UTSW	5	30,383,654 (GRCm38)	missense	probably damaging	1.00
R9783:Otof	UTSW	5	30,379,232 (GRCm38)	missense	probably benign
Z1176:Otof	UTSW	5	30,371,586 (GRCm38)	missense	probably damaging	0.98
Z1177:Otof	UTSW	5	30,383,658 (GRCm38)	missense	probably damaging	1.00
Z1177:Otof	UTSW	5	30,376,297 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAATACATGTTTATGTGGATGCAC -3'
(R):5'- CCAGAAACTCCTGCTTGGTG -3'

Sequencing Primer
(F):5'- ACATGTTTATGTGGATGCACATATG -3'
(R):5'- CTGCTTGGTGGAGGTCTCTCAC -3'

Posted On

2016-07-06