|Institutional Source||Beutler Lab|
|Gene Name||D-amino acid oxidase|
|Synonyms||Dao-1, DAO, Dao1|
|Is this an essential gene?||Probably non essential (E-score: 0.053)|
|Stock #||R5176 (G1)|
|Chromosomal Location||114003703-114025682 bp(+) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||T to C at 114020009 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000125588 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000086599] [ENSMUST00000112292] [ENSMUST00000161610]|
|AlphaFold||no structure available at present|
|Meta Mutation Damage Score||0.9491|
|Coding Region Coverage||
|Validation Efficiency||99% (69/70)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mice display increased levels of D-serine and a decrease in the severity of behavioral effects induced by NMDA receptor antagonists. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dao||
(F):5'- TCCAGAAGTCCTAGGTCAGAGG -3'
(R):5'- ACTGCCTGTGTATTCAGTCC -3'
(F):5'- AGTCCTAGGTCAGAGGGGTCAC -3'
(R):5'- GGCCTTACCACTTCTGCTACCAG -3'