Mutagenetix > Incidental Mutation

Incidental Mutation 'R5254:Vmn2r25'

399339

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r25

Ensembl Gene

ENSMUSG00000094672

Gene Name

vomeronasal 2, receptor 25

Synonyms

EG545874

MMRRC Submission

042825-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.125) question?

Stock #

R5254 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

123822814-123853190 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to G at 123825318 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 542 (C542S)

Ref Sequence

ENSEMBL: ENSMUSP00000124342 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000162046]

AlphaFold

W4VSP2

Predicted Effect

probably damaging
Transcript: ENSMUST00000162046
AA Change: C542S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000124342
Gene: ENSMUSG00000094672
AA Change: C542S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	473	6e-31	PFAM
Pfam:NCD3G	519	572	5.8e-25	PFAM
Pfam:7tm_3	603	840	4.8e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.6%

Validation Efficiency

98% (82/84)

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd8	G	T	8: 71,458,398	C296*	probably null	Het
Adam11	T	A	11: 102,774,272	Y413*	probably null	Het
Ankhd1	A	G	18: 36,656,715	I907V	probably benign	Het
Arrdc5	A	G	17: 56,297,897	I130T	probably benign	Het
Asic5	T	A	3: 82,020,987	I419K	probably damaging	Het
Atp4a	A	T	7: 30,715,530	E248V	probably damaging	Het
Avil	C	A	10: 127,011,761	V154L	probably benign	Het
Bclaf1	T	A	10: 20,323,536	H226Q	possibly damaging	Het
Cald1	A	G	6: 34,746,416		probably benign	Het
Cd200r4	A	C	16: 44,832,090	D27A	possibly damaging	Het
Cdsn	T	A	17: 35,552,202	M1K	probably null	Het
Cfap46	T	A	7: 139,678,514	H281L	possibly damaging	Het
Chaf1a	T	C	17: 56,062,606	F533L	probably benign	Het
Chil4	T	A	3: 106,219,452	I5F	probably benign	Het
Ctu2	A	T	8: 122,476,588	R48W	probably damaging	Het
Daam1	A	T	12: 71,946,576	H373L	unknown	Het
Dcaf10	T	A	4: 45,370,415	Y328N	possibly damaging	Het
Dst	A	T	1: 34,177,931	K1151*	probably null	Het
Ect2	T	C	3: 27,130,070	D503G	probably damaging	Het
Epm2a	C	A	10: 11,457,345	D307E	probably benign	Het
Exph5	A	T	9: 53,337,930	D73V	probably damaging	Het
Fam20b	C	T	1: 156,705,740	G102D	probably damaging	Het
Fat2	T	C	11: 55,281,175	N2904S	probably damaging	Het
Flt3	T	A	5: 147,375,690	Q147L	possibly damaging	Het
Fndc11	A	G	2: 181,222,163	T254A	possibly damaging	Het
Galnt15	C	T	14: 32,058,287	R514*	probably null	Het
Gbgt1	A	G	2: 28,505,208	D286G	probably damaging	Het
Ggt1	T	A	10: 75,579,198		probably null	Het
Gm26526	A	T	7: 39,589,234		noncoding transcript	Het
H2-K1	T	C	17: 33,997,462	T237A	probably damaging	Het
Igf1r	T	A	7: 68,207,319	S1010T	probably damaging	Het
Il21	T	C	3: 37,227,735	T87A	possibly damaging	Het
Kmt2b	G	A	7: 30,569,175	R2010C	probably damaging	Het
Kmt2c	G	A	5: 25,314,594	P2173S	probably benign	Het
Krt1	A	T	15: 101,846,368	S512T	unknown	Het
Krtap16-1	G	A	11: 99,985,598	R327*	probably null	Het
Lama1	T	A	17: 67,756,716	I745N	probably benign	Het
Lrrk1	T	A	7: 66,307,107	N372I	probably benign	Het
Lyst	A	T	13: 13,683,070	E2481D	probably benign	Het
Map2k1	A	T	9: 64,187,745		probably benign	Het
Mbip	A	G	12: 56,337,443	V215A	probably damaging	Het
Mdc1	T	A	17: 35,847,922	V398D	probably benign	Het
Mog	T	G	17: 37,012,372	I225L	probably benign	Het
Mrgprx3-ps	T	A	7: 47,309,436		noncoding transcript	Het
Muc5b	C	T	7: 141,864,540	S3741L	probably benign	Het
Myo5a	A	T	9: 75,130,120	I202F	probably damaging	Het
Myo5b	A	T	18: 74,700,606	I818F	possibly damaging	Het
Nfia	T	A	4: 98,014,297	M262K	probably damaging	Het
Nisch	C	T	14: 31,206,567		probably null	Het
Nkd1	A	G	8: 88,589,194	D64G	probably damaging	Het
Nt5c2	T	A	19: 46,893,560	K284*	probably null	Het
Olfr1052	A	T	2: 86,297,921	Y35F	probably damaging	Het
Olfr1058	A	T	2: 86,386,140	S93T	possibly damaging	Het
Olfr262	A	G	19: 12,241,248	S138P	probably damaging	Het
Olfr305	A	T	7: 86,364,190	V49E	possibly damaging	Het
Olfr619	T	G	7: 103,603,789	I45R	probably benign	Het
Olfr723	A	T	14: 49,928,779	I255N	probably damaging	Het
Olfr725	C	A	14: 50,034,678	A242S	possibly damaging	Het
Olfr972	A	T	9: 39,873,445	T57S	possibly damaging	Het
Pcdhb17	A	T	18: 37,486,825	D556V	probably damaging	Het
Pcdhga8	A	T	18: 37,726,620	D243V	probably benign	Het
Polq	A	T	16: 37,089,319	Q2355L	probably damaging	Het
Slc22a30	G	A	19: 8,344,393	Q436*	probably null	Het
Slc5a4a	C	A	10: 76,182,738	Y506*	probably null	Het
Sp110	G	C	1: 85,577,202		probably benign	Het
Tarbp1	G	A	8: 126,467,156	H336Y	probably damaging	Het
Tas2r134	T	G	2: 51,627,547	F13V	probably benign	Het
Tgfb2	A	G	1: 186,704,483	Y98H	probably damaging	Het
Tmprss11a	C	A	5: 86,411,806	V376L	probably damaging	Het
Tmprss11f	T	A	5: 86,538,033	K158N	probably benign	Het
Tnip2	G	T	5: 34,503,578	Q177K	probably damaging	Het
Trp53inp1	T	A	4: 11,165,075		probably null	Het
Ttc28	C	T	5: 111,271,238	P1398S	probably benign	Het
Umodl1	T	G	17: 30,980,359	I308S	possibly damaging	Het
Vcan	A	T	13: 89,691,600	S1942T	probably damaging	Het
Vmn2r124	T	A	17: 18,063,077	N344K	probably benign	Het
Wiz	T	A	17: 32,378,496		probably benign	Het
Wrap73	A	G	4: 154,155,346	Y343C	probably benign	Het

Other mutations in Vmn2r25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Vmn2r25	APN	6	123853171	missense	probably benign	0.25
IGL01781:Vmn2r25	APN	6	123839365	missense	possibly damaging	0.48
IGL01843:Vmn2r25	APN	6	123853003	missense	possibly damaging	0.67
IGL02023:Vmn2r25	APN	6	123839429	missense	probably damaging	0.96
IGL02502:Vmn2r25	APN	6	123839433	missense	probably damaging	0.96
IGL02709:Vmn2r25	APN	6	123839764	missense	possibly damaging	0.50
IGL03053:Vmn2r25	APN	6	123823118	missense	probably damaging	1.00
PIT4468001:Vmn2r25	UTSW	6	123839598	missense	probably benign	0.00
PIT4812001:Vmn2r25	UTSW	6	123823488	missense	probably damaging	1.00
R0054:Vmn2r25	UTSW	6	123853025	missense	probably benign	0.00
R0312:Vmn2r25	UTSW	6	123828580	splice site	probably benign
R0366:Vmn2r25	UTSW	6	123823622	nonsense	probably null
R0390:Vmn2r25	UTSW	6	123823181	missense	probably damaging	1.00
R0466:Vmn2r25	UTSW	6	123852049	missense	probably benign	0.16
R0541:Vmn2r25	UTSW	6	123839827	missense	probably damaging	0.97
R0612:Vmn2r25	UTSW	6	123839522	missense	probably damaging	1.00
R0865:Vmn2r25	UTSW	6	123853017	missense	probably benign	0.09
R1219:Vmn2r25	UTSW	6	123839323	missense	probably benign	0.00
R1240:Vmn2r25	UTSW	6	123851905	missense	probably damaging	0.98
R1701:Vmn2r25	UTSW	6	123851795	splice site	probably null
R1780:Vmn2r25	UTSW	6	123828465	missense	probably damaging	1.00
R1809:Vmn2r25	UTSW	6	123825378	missense	probably benign	0.00
R1833:Vmn2r25	UTSW	6	123839684	missense	probably benign	0.01
R1964:Vmn2r25	UTSW	6	123823295	missense	possibly damaging	0.94
R2154:Vmn2r25	UTSW	6	123839846	missense	probably benign	0.01
R2164:Vmn2r25	UTSW	6	123839559	missense	possibly damaging	0.96
R3799:Vmn2r25	UTSW	6	123853184	missense	probably benign	0.12
R3836:Vmn2r25	UTSW	6	123853085	missense	probably damaging	1.00
R3946:Vmn2r25	UTSW	6	123840098	missense	probably damaging	0.97
R4282:Vmn2r25	UTSW	6	123823647	missense	probably damaging	1.00
R4367:Vmn2r25	UTSW	6	123828537	missense	probably damaging	1.00
R4438:Vmn2r25	UTSW	6	123839797	missense	probably benign	0.03
R4488:Vmn2r25	UTSW	6	123822860	missense	probably damaging	1.00
R4580:Vmn2r25	UTSW	6	123823023	missense	possibly damaging	0.46
R4631:Vmn2r25	UTSW	6	123853003	missense	possibly damaging	0.94
R4765:Vmn2r25	UTSW	6	123823223	missense	probably damaging	1.00
R4908:Vmn2r25	UTSW	6	123828447	missense	probably benign
R5207:Vmn2r25	UTSW	6	123840103	missense	probably damaging	1.00
R5444:Vmn2r25	UTSW	6	123828492	missense	probably benign	0.00
R5586:Vmn2r25	UTSW	6	123825296	missense	probably damaging	1.00
R5607:Vmn2r25	UTSW	6	123828359	missense	possibly damaging	0.49
R5985:Vmn2r25	UTSW	6	123823628	missense	probably benign
R6046:Vmn2r25	UTSW	6	123822917	missense	probably damaging	1.00
R6057:Vmn2r25	UTSW	6	123822941	missense	possibly damaging	0.69
R6569:Vmn2r25	UTSW	6	123851982	missense	probably benign	0.01
R6826:Vmn2r25	UTSW	6	123823112	missense	probably damaging	1.00
R7054:Vmn2r25	UTSW	6	123823610	missense	probably damaging	1.00
R7120:Vmn2r25	UTSW	6	123828435	missense	possibly damaging	0.51
R7177:Vmn2r25	UTSW	6	123839923	missense	possibly damaging	0.94
R7287:Vmn2r25	UTSW	6	123852081	missense	possibly damaging	0.49
R7397:Vmn2r25	UTSW	6	123823539	missense	probably damaging	0.96
R7486:Vmn2r25	UTSW	6	123823142	missense	probably damaging	1.00
R7699:Vmn2r25	UTSW	6	123839923	missense	possibly damaging	0.94
R7700:Vmn2r25	UTSW	6	123839923	missense	possibly damaging	0.94
R7759:Vmn2r25	UTSW	6	123823380	missense	probably damaging	0.99
R7802:Vmn2r25	UTSW	6	123851832	missense	possibly damaging	0.88
R7850:Vmn2r25	UTSW	6	123828472	missense	probably damaging	1.00
R8064:Vmn2r25	UTSW	6	123823622	nonsense	probably null
R8170:Vmn2r25	UTSW	6	123853017	missense	probably benign	0.09
R8340:Vmn2r25	UTSW	6	123853013	missense	probably benign	0.01
R8346:Vmn2r25	UTSW	6	123825391	missense	probably benign	0.00
R8395:Vmn2r25	UTSW	6	123823023	missense	possibly damaging	0.81
R8889:Vmn2r25	UTSW	6	123823610	missense	probably damaging	1.00
R9094:Vmn2r25	UTSW	6	123828432	missense	probably benign	0.44
R9204:Vmn2r25	UTSW	6	123853133	missense	probably benign	0.00
R9253:Vmn2r25	UTSW	6	123840001	missense	probably damaging	0.98
R9392:Vmn2r25	UTSW	6	123839978	missense	probably benign
R9520:Vmn2r25	UTSW	6	123853107	nonsense	probably null
R9525:Vmn2r25	UTSW	6	123823205	missense	probably damaging	1.00
R9622:Vmn2r25	UTSW	6	123839620	missense	probably damaging	0.99
X0020:Vmn2r25	UTSW	6	123839400	missense	possibly damaging	0.95
Z1176:Vmn2r25	UTSW	6	123822897	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGGTGCATCATTTCCACATTC -3'
(R):5'- TCTCTAAAAGCATGGTAGTTCTTGG -3'

Sequencing Primer
(F):5'- GGTGCATCATTTCCACATTCAGAATC -3'
(R):5'- GGTTGTTACAGTACACAGATGCCC -3'

Posted On

2016-07-06